BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0880 (498 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At4g21705.1 68417.m03143 pentatricopeptide (PPR) repeat-containi... 30 0.75 At3g61670.1 68416.m06911 expressed protein weak similarity to ex... 30 1.00 At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 28 3.0 At5g13020.1 68418.m01492 emsy N terminus domain-containing prote... 28 4.0 At2g18880.1 68415.m02203 fibronectin type III domain-containing ... 27 5.3 At5g11100.1 68418.m01296 C2 domain-containing protein similar to... 27 7.0 At4g37440.1 68417.m05299 expressed protein 27 7.0 At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note:... 27 7.0 At5g60210.1 68418.m07547 cytoplasmic linker protein-related cont... 27 9.3 At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro... 27 9.3 At2g37070.1 68415.m04549 expressed protein 27 9.3 >At4g21705.1 68417.m03143 pentatricopeptide (PPR) repeat-containing protein low similarity to DNA-binding protein [Triticum aestivum] GI:6958202; contains Pfam profile PF01535: PPR repeat Length = 492 Score = 30.3 bits (65), Expect = 0.75 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +1 Query: 238 DKQKQMAKQLAQGIKKTHPELWDEFITFYDPQGKYQTSFK 357 +K + M + LA+ K T PE W+ T Y +G + +FK Sbjct: 354 EKAEAMLEDLARRGKATTPESWELVATAYAEKGTLENAFK 393 >At3g61670.1 68416.m06911 expressed protein weak similarity to extra-large G-protein [Arabidopsis thaliana] GI:3201682 Length = 790 Score = 29.9 bits (64), Expect = 1.00 Identities = 16/38 (42%), Positives = 20/38 (52%), Gaps = 2/38 (5%) Frame = +1 Query: 187 ELKKIKEALETHCAKCTDKQKQMA--KQLAQGIKKTHP 294 +L+KIKE L C TDK K+ A A G+ K P Sbjct: 201 QLEKIKEQLVQSCNVATDKSKEQAPSSSSASGLNKAPP 238 >At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly identical to auxin transport protein; BIG [Arabidopsis thaliana] GI:21779966; contains Pfam profiles PF02207: Putative zinc finger in N-recognin, PF00569: Zinc finger ZZ type Length = 5098 Score = 28.3 bits (60), Expect = 3.0 Identities = 13/33 (39%), Positives = 20/33 (60%) Frame = +1 Query: 85 EPIDDSFDASEVLSNERLLKSYTKCLLNQGPCT 183 EP+D + V+S+ L+ SY +CL +QG T Sbjct: 2496 EPLDTPTMNNVVMSSVELIYSYAECLASQGKDT 2528 >At5g13020.1 68418.m01492 emsy N terminus domain-containing protein / ENT domain-containing protein contains Pfam profile PF03735: ENT domain Length = 397 Score = 27.9 bits (59), Expect = 4.0 Identities = 14/36 (38%), Positives = 18/36 (50%) Frame = +1 Query: 115 EVLSNERLLKSYTKCLLNQGPCTAELKKIKEALETH 222 E+L E LLK K + P AE++K K L H Sbjct: 340 EILHTETLLKEVEKVFGSVNPNPAEVEKAKRVLRDH 375 >At2g18880.1 68415.m02203 fibronectin type III domain-containing protein contains Pfam profile PF00041: Fibronectin type III domain Length = 417 Score = 27.5 bits (58), Expect = 5.3 Identities = 18/59 (30%), Positives = 28/59 (47%), Gaps = 3/59 (5%) Frame = +1 Query: 160 LLNQGPCTAELKKI--KEALETHCAKCTDKQKQMAKQLAQGIKKTHPELWDEFI-TFYD 327 L+ Q C+ ++K K+ L +C K TDK+K + + K L + I TF D Sbjct: 334 LIRQLECSGQVKSDFRKKFLTWYCLKATDKEKHVVEIFVDTFKDDKEALAKQLIDTFSD 392 >At5g11100.1 68418.m01296 C2 domain-containing protein similar to Ca2+-dependent lipid-binding protein (CLB1) GI:2789434 from [Lycopersicon esculentum] Length = 574 Score = 27.1 bits (57), Expect = 7.0 Identities = 17/43 (39%), Positives = 24/43 (55%) Frame = +1 Query: 73 QDKYEPIDDSFDASEVLSNERLLKSYTKCLLNQGPCTAELKKI 201 QD + + F S V+ ++R SY+KCLLN EL+KI Sbjct: 48 QDSRKLLPGDFYPSWVVFSQRQKLSYSKCLLNW--LNLELEKI 88 >At4g37440.1 68417.m05299 expressed protein Length = 471 Score = 27.1 bits (57), Expect = 7.0 Identities = 13/36 (36%), Positives = 18/36 (50%) Frame = +2 Query: 194 RKLKKLWRPIVRNVLINRSRWRNNLRKELRRHTRSY 301 RKL WR V+ L+ R +W KEL+ + Y Sbjct: 130 RKLTDHWRRFVQPTLMWRCKWIELKYKELQNQAQKY 165 >At1g08140.1 68414.m00896 cation/hydrogen exchanger (CHX6a) Note: CHX6a and CHX6b were originally 1 gene but were split pased on alignments with other family members; may be a pseudogene and requires futher investigation; monovalent cation:proton antiporter family 2 (CPA2) member, PMID:11500563 Length = 818 Score = 27.1 bits (57), Expect = 7.0 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 1/49 (2%) Frame = +1 Query: 100 SFDASEVLSNERLLKSYTKCLLNQGPCTAELKKI-KEALETHCAKCTDK 243 S D S V+SN+ L+ +L + PC+ + K +E+H AK K Sbjct: 606 SVDRSTVISNDDNLRMLNVNVLRRAPCSVGIFVYRKPIVESHMAKSHSK 654 >At5g60210.1 68418.m07547 cytoplasmic linker protein-related contains weak similarity to cytoplasmic linker protein CLIP-170 (GI:2905649) [Gallus gallus] Length = 588 Score = 26.6 bits (56), Expect = 9.3 Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 6/50 (12%) Frame = +1 Query: 184 AELKKIKEALETHCAKCTDKQKQMA------KQLAQGIKKTHPELWDEFI 315 AELKK++EA+E A DK+ ++ + L I K+ ++ D F+ Sbjct: 433 AELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSETDVQDAFL 482 >At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family protein contains Pfam profile PF00179: Ubiquitin-conjugating enzyme Length = 362 Score = 26.6 bits (56), Expect = 9.3 Identities = 18/62 (29%), Positives = 29/62 (46%), Gaps = 3/62 (4%) Frame = +1 Query: 142 KSYTKCLLNQGPCTAELKKIKE---ALETHCAKCTDKQKQMAKQLAQGIKKTHPELWDEF 312 K Y + +LN AEL+K+KE L + + Q GIKKT W+++ Sbjct: 298 KVYCEQILNSD-LKAELEKLKEKEYTLSDYYYGSSSSNYPTYTQRDDGIKKTSKSFWNKY 356 Query: 313 IT 318 ++ Sbjct: 357 LS 358 >At2g37070.1 68415.m04549 expressed protein Length = 420 Score = 26.6 bits (56), Expect = 9.3 Identities = 16/32 (50%), Positives = 18/32 (56%) Frame = -2 Query: 143 LRSLSLLKTSEASKLSSIGSYLS*ASTAKNRP 48 LRS K S SSI S LS +STA N+P Sbjct: 173 LRSSVASKNELTSSCSSIESCLSVSSTASNKP 204 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 9,485,404 Number of Sequences: 28952 Number of extensions: 174939 Number of successful extensions: 478 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 472 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 476 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 878448512 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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