Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0859
(799 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65430.1 68414.m07423 zinc finger protein-related contains we... 32 0.38
At5g58040.1 68418.m07263 fip1 motif-containing protein contains ... 31 0.67
At4g28890.1 68417.m04129 zinc finger (C3HC4-type RING finger) fa... 30 1.5
At4g21705.1 68417.m03143 pentatricopeptide (PPR) repeat-containi... 30 1.5
At5g44080.1 68418.m05393 bZIP transcription factor family protei... 29 2.7
At1g77840.1 68414.m09070 eukaryotic translation initiation facto... 29 2.7
At5g11100.1 68418.m01296 C2 domain-containing protein similar to... 29 4.7
At5g63550.1 68418.m07976 expressed protein 28 6.2
At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly id... 28 6.2
At2g26660.1 68415.m03198 SPX (SYG1/Pho81/XPR1) domain-containing... 28 6.2
At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family pro... 28 8.3
At2g03630.1 68415.m00323 hypothetical protein 28 8.3
At1g17310.1 68414.m02110 MADS-box protein (AGL100) similar to tr... 28 8.3
>At1g65430.1 68414.m07423 zinc finger protein-related contains weak
similarity to zinc finger proteins and a Pfam:PF01485
IBR domain
Length = 567
Score = 32.3 bits (70), Expect = 0.38
Identities = 19/46 (41%), Positives = 24/46 (52%)
Frame = +2
Query: 569 DRKHKDSRIMEEMQGLEELESKLSAYHVMVEKEVLGKRDARSPSLD 706
D++H E +QG E ES L H EKE+L DA+ PS D
Sbjct: 434 DQEHGKRVFFEYLQG--EAESGLERLHQCAEKELLPYLDAKGPSED 477
>At5g58040.1 68418.m07263 fip1 motif-containing protein contains Pfam
profile PF05182: Fip1 motif
Length = 1192
Score = 31.5 bits (68), Expect = 0.67
Identities = 23/74 (31%), Positives = 34/74 (45%), Gaps = 2/74 (2%)
Frame = +2
Query: 569 DRKHKDSR--IMEEMQGLEELESKLSAYHVMVEKEVLGKRDARSPSLDQVPAPPLDKPKW 742
DRKHKDSR I E+ +G E L + V G DAR+ ++P P +
Sbjct: 976 DRKHKDSRRKIKEQREGTESLSKQGEQNGSSVVTGSKGTNDARN-CRSEIPHQPNTAKRH 1034
Query: 743 KMSMNAVKELGQEK 784
K + ++ E+ K
Sbjct: 1035 KENASSGDEIHDSK 1048
>At4g28890.1 68417.m04129 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097 zinc
finger, C3HC4 type (RING finger)
Length = 386
Score = 30.3 bits (65), Expect = 1.5
Identities = 20/72 (27%), Positives = 36/72 (50%), Gaps = 1/72 (1%)
Frame = +2
Query: 455 KNRPRKTNSSFILKFSTRFPSTSDAGK-SKRDHSSDGEYDRKHKDSRIMEEMQGLEELES 631
KNR +SS ++ ++ ++ + + S DH G+ + + RI EEM+ LE+
Sbjct: 223 KNRWSNVSSSDLMFLNSEMVNSISSERFSSLDHVKRGDEEDQIGILRIKEEMEAKRMLEN 282
Query: 632 KLSAYHVMVEKE 667
KL++ M E
Sbjct: 283 KLTSMTTMFSSE 294
>At4g21705.1 68417.m03143 pentatricopeptide (PPR) repeat-containing
protein low similarity to DNA-binding protein [Triticum
aestivum] GI:6958202; contains Pfam profile PF01535: PPR
repeat
Length = 492
Score = 30.3 bits (65), Expect = 1.5
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = -2
Query: 264 DKQKQMAKQLAQGIKKTHPELWDEFITFYDPQGKYQTSFK 145
+K + M + LA+ K T PE W+ T Y +G + +FK
Sbjct: 354 EKAEAMLEDLARRGKATTPESWELVATAYAEKGTLENAFK 393
>At5g44080.1 68418.m05393 bZIP transcription factor family protein
contains Pfam profile: PF00170 bZIP transcription factor
Length = 315
Score = 29.5 bits (63), Expect = 2.7
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +2
Query: 614 LEELESKLSAYHVMVEKEVLGKRDARSPSLDQVPAPPLDKPK 739
LE L +KL + ++ KE+ KR R L + P ++KPK
Sbjct: 259 LEALAAKLEEENELLSKEIEDKRKERYQKLMEFVIPVVEKPK 300
>At1g77840.1 68414.m09070 eukaryotic translation initiation factor
5, putative / eIF-5, putative similar to SP|P55876
Eukaryotic translation initiation factor 5 (eIF-5) {Zea
mays}; contains Pfam profiles PF02020:
eIF4-gamma/eIF5/eIF2-epsilon, PF01873: Domain found in
IF2B/IF5
Length = 437
Score = 29.5 bits (63), Expect = 2.7
Identities = 22/97 (22%), Positives = 45/97 (46%), Gaps = 5/97 (5%)
Frame = +2
Query: 491 LKFSTRFPSTSDAGKSKRDHSSDGEYDRKHKDS--RIMEEMQGLEELESKLSAYHVMVEK 664
LK + S K+ ++HSSD + KH ++ + E+ + +E + EK
Sbjct: 180 LKAKKKALSNGKDSKTSKNHSSDEDISPKHDENALEVDEDEDDDDGVEWQTDTSREAAEK 239
Query: 665 EVLGKRDARSPSLDQVPAPPLDK---PKWKMSMNAVK 766
++ + A++ + + A +++ PK K + N VK
Sbjct: 240 RMMEQLSAKTAEMVMLSAMEVEEKKAPKSKSNGNVVK 276
>At5g11100.1 68418.m01296 C2 domain-containing protein similar to
Ca2+-dependent lipid-binding protein (CLB1) GI:2789434
from [Lycopersicon esculentum]
Length = 574
Score = 28.7 bits (61), Expect = 4.7
Identities = 19/50 (38%), Positives = 27/50 (54%)
Frame = -2
Query: 441 QDKYEPIDDSFDASEVLSNERLLKSYTKCLLNQGPCTAELKKIKDKIPEA 292
QD + + F S V+ ++R SY+KCLLN EL+KI + EA
Sbjct: 48 QDSRKLLPGDFYPSWVVFSQRQKLSYSKCLLNW--LNLELEKIWPYVNEA 95
>At5g63550.1 68418.m07976 expressed protein
Length = 530
Score = 28.3 bits (60), Expect = 6.2
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +2
Query: 512 PSTSDAGKSKRDHSSDGEYDRKHKDSRIMEEMQGLEELESKLSAYHVMVEK 664
PS ++ GK + D S+G D +D EE E+ ++ V VEK
Sbjct: 285 PSDTEEGKDEGDADSEGTNDPHEEDDAAPEEESDHEKTDTDDEKDEVEVEK 335
>At3g02260.1 68416.m00207 auxin transport protein (BIG) nearly
identical to auxin transport protein; BIG [Arabidopsis
thaliana] GI:21779966; contains Pfam profiles PF02207:
Putative zinc finger in N-recognin, PF00569: Zinc finger
ZZ type
Length = 5098
Score = 28.3 bits (60), Expect = 6.2
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = -2
Query: 429 EPIDDSFDASEVLSNERLLKSYTKCLLNQGPCT 331
EP+D + V+S+ L+ SY +CL +QG T
Sbjct: 2496 EPLDTPTMNNVVMSSVELIYSYAECLASQGKDT 2528
>At2g26660.1 68415.m03198 SPX (SYG1/Pho81/XPR1) domain-containing
protein low similarity to NUC-2 [Neurospora crassa]
GI:1399532, xenotropic and polytropic murine leukemia
virus receptor [Mus musculus castaneus] GI:6093320;
contains Pfam profile PF03105: SPX domain
Length = 287
Score = 28.3 bits (60), Expect = 6.2
Identities = 25/89 (28%), Positives = 42/89 (47%)
Frame = +2
Query: 512 PSTSDAGKSKRDHSSDGEYDRKHKDSRIMEEMQGLEELESKLSAYHVMVEKEVLGKRDAR 691
P+TS K+ D D E R K+ +E M+ L ++S +SA V+ E G
Sbjct: 204 PTTSGMVKTGTD---DSELLRVPKELSEIEYMESLY-MKSTVSALKVLKEIRS-GSSTVS 258
Query: 692 SPSLDQVPAPPLDKPKWKMSMNAVKELGQ 778
SL +PA L+ WK + ++++ +
Sbjct: 259 VFSLPPLPASGLEDDSWKKKVGVLEQVAK 287
>At3g12775.1 68416.m01595 ubiquitin-conjugating enzyme family
protein contains Pfam profile PF00179:
Ubiquitin-conjugating enzyme
Length = 362
Score = 27.9 bits (59), Expect = 8.3
Identities = 18/63 (28%), Positives = 30/63 (47%)
Frame = -2
Query: 372 KSYTKCLLNQGPCTAELKKIKDKIPEALETHCAKCTDKQKQMAKQLAQGIKKTHPELWDE 193
K Y + +LN AEL+K+K+K L + + Q GIKKT W++
Sbjct: 298 KVYCEQILNSD-LKAELEKLKEK-EYTLSDYYYGSSSSNYPTYTQRDDGIKKTSKSFWNK 355
Query: 192 FIT 184
+++
Sbjct: 356 YLS 358
>At2g03630.1 68415.m00323 hypothetical protein
Length = 252
Score = 27.9 bits (59), Expect = 8.3
Identities = 20/72 (27%), Positives = 32/72 (44%)
Frame = +2
Query: 386 LLKTSEASKLSSIGSYLS*ASTAKNRPRKTNSSFILKFSTRFPSTSDAGKSKRDHSSDGE 565
++ + S+LSS S S +S++ P K +LK S P S D ++G+
Sbjct: 46 IINSESHSRLSSSSSSSS-SSSSYLSPPKDLPEEVLKESLNDPEISSPPVQVMDRDNNGK 104
Query: 566 YDRKHKDSRIME 601
YD S + E
Sbjct: 105 YDPNRIPSSVFE 116
>At1g17310.1 68414.m02110 MADS-box protein (AGL100) similar to
transcription factor GB:BAA25245 GI:2981610 from
[Ceratopteris richardii]; contains Pfam profile PF00319:
SRF-type transcription factor (DNA-binding and
dimerisation domain)
Length = 217
Score = 27.9 bits (59), Expect = 8.3
Identities = 22/76 (28%), Positives = 41/76 (53%), Gaps = 2/76 (2%)
Frame = +2
Query: 545 DHSSDGEYDRKHKDSRIMEEM--QGLEELESKLSAYHVMVEKEVLGKRDARSPSLDQVPA 718
D+++D E R+ R +E + + LEE + LS + K+++ + R+ +D VPA
Sbjct: 127 DNAADEE-SRRPWWERPVESVPEEELEEYMAALSMLRENIGKKIVAMGNDRT--VDMVPA 183
Query: 719 PPLDKPKWKMSMNAVK 766
P++ WK +M+ K
Sbjct: 184 WPINVMGWKPTMDMQK 199
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,958,982
Number of Sequences: 28952
Number of extensions: 310876
Number of successful extensions: 1020
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 994
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1020
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1804564000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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