Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0857
(813 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 194 4e-50
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 194 6e-50
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 192 2e-49
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 188 5e-48
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 127 1e-29
At4g02660.1 68417.m00361 WD-40 repeat family protein / beige-rel... 30 1.6
At1g54860.1 68414.m06263 expressed protein 28 6.4
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 194 bits (474), Expect = 4e-50
Identities = 95/206 (46%), Positives = 131/206 (63%)
Frame = +3
Query: 168 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 347
PAF G+ G +IWRI+NFE +PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 348 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 527
G++++QDE+G AA+ TV LD GG AVQHRE GHES FLSYF+ + LEGG SGF
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGGVASGF 127
Query: 528 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKAKNVE 707
V + R++ KGKR +R++QV +S+N DVF+LD + IY F G + E
Sbjct: 128 K-TVEEEVFETRLYTCKGKRAIRLKQVPFARSSLNHDDVFILDTEEKIYQFNGANSNIQE 186
Query: 708 KLKAISFANQVRDQDHHGRGRVDIVD 785
+ KA+ ++D+ H G V IVD
Sbjct: 187 RAKALEVVQYLKDKYHEGTCDVAIVD 212
Score = 65.3 bits (152), Expect = 5e-11
Identities = 42/175 (24%), Positives = 80/175 (45%), Gaps = 10/175 (5%)
Frame = +3
Query: 201 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGA 380
+++W + P+ ++DIGK Y GD Y++L T + + W G++S ++
Sbjct: 392 LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGERKDEYFLSCWFGKKSIPEDQDT 451
Query: 381 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGAQK 560
A L + + G VQ R G E F++ FQ P+ L+GG SG+ + + +
Sbjct: 452 AIRLANTMSNSLKGRPVQGRIYEGKEPPQFVALFQ-PMVVLKGGLSSGYKSSMGESESTD 510
Query: 561 RMF--------QVKGK--RDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKA 695
+ QV G + + QV+ S+N + F+L ++++ G ++
Sbjct: 511 ETYTPESIALVQVSGTGVHNNKAVQVETVATSLNSYECFLLQSGTSMFLWHGNQS 565
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 194 bits (473), Expect = 6e-50
Identities = 91/205 (44%), Positives = 134/205 (65%)
Frame = +3
Query: 171 AFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIG 350
A G+ +G++IWRI+NF+P+ V Q+ GKF+ GDSYI+L+TT+ +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 351 RESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFN 530
++S+QDE+GA A++TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SGFN
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGFN 130
Query: 531 HVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKAKNVEK 710
HV Q R++ KGK VRV++V +++N DVF+LD ++ I+ F G K+ E+
Sbjct: 131 HVKPEE-HQTRLYICKGKHVVRVKEVPFVRSTLNHEDVFILDTESKIFQFSGSKSSIQER 189
Query: 711 LKAISFANQVRDQDHHGRGRVDIVD 785
KA+ ++D H G+ + V+
Sbjct: 190 AKALEVVQYIKDTYHDGKCDIAAVE 214
Score = 52.4 bits (120), Expect = 3e-07
Identities = 46/216 (21%), Positives = 91/216 (42%), Gaps = 10/216 (4%)
Frame = +3
Query: 69 GSVWARTATKPQEISGPITSLSDKNARDKAKVHPAFANVGRTAGVQIWRIQNFEPIPVAQ 248
G V A + + G + S +++D+ K + + T +Q+WRI E I +
Sbjct: 358 GKVAALLQRQGVNVQG-LVKTSSSSSKDEPKPY-----IDGTGNLQVWRINCEEKILLEA 411
Query: 249 KDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGAAAILTVGLDDKFGGAA 428
+ KFY GD Y IL+ + + + W G++S +++ +A L + +
Sbjct: 412 AEQSKFYSGDCY-ILQYSYPGEDREEHLVGTWFGKQSVEEDRASAISLANKMVESMKFVP 470
Query: 429 VQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVV--------TNAGAQKRMFQVKGK 584
Q R G E F Q+ + + +GG F + T +F+V+G
Sbjct: 471 AQARINEGKEPIQFFVIMQSFITF-KGGVSDAFKKYIAENDIPDTTYEAEGVALFRVQGS 529
Query: 585 --RDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 686
+++ Q++ A +N ++L D+ ++ + G
Sbjct: 530 GPENMQAIQIEAASAGLNSSHCYILHGDSTVFTWCG 565
Score = 27.9 bits (59), Expect = 8.5
Identities = 10/38 (26%), Positives = 21/38 (55%)
Frame = +3
Query: 639 DVFVLDLDNDIYVFVGEKAKNVEKLKAISFANQVRDQD 752
D+F+LD +++V+VG++ +K +A+ D
Sbjct: 653 DIFILDCHTEVFVWVGQQVDPKKKPQALDIGENFLKHD 690
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 192 bits (468), Expect = 2e-49
Identities = 95/206 (46%), Positives = 130/206 (63%)
Frame = +3
Query: 168 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 347
PAF VG+ G +IWRI+NFEP+PV + + GKFY GD+YI+L+TT + +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 348 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 527
G++++QDE+G AA+ TV LD GG AVQ+RE GHES FLSYF+ + LEGG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGGVASGF 129
Query: 528 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKAKNVE 707
+ R++ KGKR V ++QV +S+N DVF+LD IY F G + E
Sbjct: 130 KK-PEEEEFETRLYTCKGKRAVHLKQVPFARSSLNHDDVFILDTKEKIYQFNGANSNIQE 188
Query: 708 KLKAISFANQVRDQDHHGRGRVDIVD 785
+ KA+ ++D+ H G V IVD
Sbjct: 189 RAKALVVIQYLKDKFHEGTSDVAIVD 214
Score = 68.1 bits (159), Expect = 6e-12
Identities = 46/187 (24%), Positives = 84/187 (44%), Gaps = 10/187 (5%)
Frame = +3
Query: 201 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDESGA 380
+++W I +++ +GK Y GD Y++L T + + W G+ S Q++
Sbjct: 394 LEVWYIDANSKTVLSKDHVGKLYSGDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQET 453
Query: 381 AAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGAQK 560
A L + + G VQ R G E F++ FQ + L+GG SG+ + +T G+
Sbjct: 454 AVRLASTMTNSLKGRPVQARIFEGKEPPQFVALFQ-HMVVLKGGLSSGYKNSMTEKGSSG 512
Query: 561 R--------MFQVKGK--RDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKAKNVEK 710
+ QV G + + QV+ S+N D F+L ++++VG + + ++
Sbjct: 513 ETYTPESIALIQVSGTGVHNNKALQVEAVATSLNSYDCFLLQSGTSMFLWVGNHSTHEQQ 572
Query: 711 LKAISFA 731
A A
Sbjct: 573 ELAAKVA 579
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 188 bits (457), Expect = 5e-48
Identities = 90/206 (43%), Positives = 128/206 (62%)
Frame = +3
Query: 168 PAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWI 347
PAF G+ AG++IWRI+NF P P+ + IGKF+ GDSYI+L+TT+ L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 348 GRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGF 527
G++++QDE+G AA+ TV LD GG AVQ+RE GHE+ FLSYF+ + EGG SGF
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGGVASGF 129
Query: 528 NHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKAKNVE 707
HVV R+F +GK V V++V +S+N D+++LD + I+ F G + E
Sbjct: 130 KHVVAEEHI-TRLFVCRGKHVVHVKEVPFARSSLNHDDIYILDTKSKIFQFNGSNSSIQE 188
Query: 708 KLKAISFANQVRDQDHHGRGRVDIVD 785
+ KA+ ++D H G V V+
Sbjct: 189 RAKALEVVQYIKDTYHDGTCEVATVE 214
Score = 58.0 bits (134), Expect = 7e-09
Identities = 41/175 (23%), Positives = 72/175 (41%), Gaps = 10/175 (5%)
Frame = +3
Query: 192 TAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHYWIGRESTQDE 371
T +Q+WR+ + D KFY GD Y + + + I W G++S ++E
Sbjct: 391 TGNLQVWRVNGQAKTLLQAADHSKFYSGDCY-VFQYSYPGEEKEEVLIGTWFGKQSVEEE 449
Query: 372 SGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNA- 548
G+A + + + Q R G E F Q+ + + +GG SG+ +
Sbjct: 450 RGSAVSMASKMVESMKFVPAQARIYEGKEPIQFFVIMQSFIVF-KGGISSGYKKYIAEKE 508
Query: 549 ---------GAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVG 686
G Q G +++ QVDP AS+N ++L D+ ++ + G
Sbjct: 509 VDDDTYNENGVALFRIQGSGPENMQAIQVDPVAASLNSSYYYILHNDSSVFTWAG 563
Score = 30.7 bits (66), Expect = 1.2
Identities = 11/38 (28%), Positives = 25/38 (65%)
Frame = +3
Query: 639 DVFVLDLDNDIYVFVGEKAKNVEKLKAISFANQVRDQD 752
D+F++D ++I+V+VG++ KL A++ + ++D
Sbjct: 651 DIFIIDCHSEIFVWVGQEVVPKNKLLALTIGEKFIEKD 688
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 127 bits (306), Expect = 1e-29
Identities = 66/208 (31%), Positives = 111/208 (53%)
Frame = +3
Query: 162 VHPAFANVGRTAGVQIWRIQNFEPIPVAQKDIGKFYKGDSYIILRTTSDNRNNLSWDIHY 341
+ AF VG +G++IW + N + I + + GKF+ G++Y++LRT + +DIHY
Sbjct: 8 IDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESPQYDIHY 67
Query: 342 WIGRESTQDESGAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPS 521
W+G ++ + +S A+ + LD G VQ+RE G E+ FLSYF+ + +EG
Sbjct: 68 WLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQETEKFLSYFKPCIIPVEGKYSP 127
Query: 522 GFNHVVTNAGAQKRMFQVKGKRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGEKAKN 701
+ Q + + KG VRV++V +S+N DVF+LD + +++F G +
Sbjct: 128 KTG--IAGETYQVTLLRCKGDHVVRVKEVPFLRSSLNHDDVFILDTASKVFLFAGCNSST 185
Query: 702 VEKLKAISFANQVRDQDHHGRGRVDIVD 785
EK KA+ ++D H GR V ++
Sbjct: 186 QEKAKAMEVVEYIKDNKHDGRCEVATIE 213
Score = 34.7 bits (76), Expect = 0.074
Identities = 36/175 (20%), Positives = 74/175 (42%), Gaps = 12/175 (6%)
Frame = +3
Query: 201 VQIWRIQNFEPIPVAQKDIGKFYKGDSYIILR--TTSDNRNNLSWDIHYWIGRESTQDES 374
+++WR+ + ++ D K + GD Y++ T + +L ++ WIG ES Q +
Sbjct: 391 LKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL---LYVWIGCESIQQDR 447
Query: 375 GAAAILTVGLDDKFGGAAVQHRETMGHESALFLSYFQTPLNYLEGGNPSGFNHVVTNAGA 554
A + G +V G+E + F FQ+ L +GG + ++
Sbjct: 448 ADAITNASAIVGTTKGESVLCHIYQGNEPSRFFPMFQS-LVVFKGGLSRRYKVLLAEKEK 506
Query: 555 --------QKRMFQVKG--KRDVRVRQVDPQIASMNKGDVFVLDLDNDIYVFVGE 689
+ +F+V G R+++ QV+ S+N ++L + ++G+
Sbjct: 507 IGEEYNENKASLFRVVGTSPRNMQAIQVNLVATSLNSSYSYILQYGASAFTWIGK 561
Score = 29.1 bits (62), Expect = 3.7
Identities = 11/38 (28%), Positives = 23/38 (60%)
Frame = +3
Query: 639 DVFVLDLDNDIYVFVGEKAKNVEKLKAISFANQVRDQD 752
DVF+LD +++YV++G + K +A++ + + D
Sbjct: 648 DVFLLDCQSEVYVWIGSNSNIKSKEEALTLGLKFLEMD 685
>At4g02660.1 68417.m00361 WD-40 repeat family protein /
beige-related contains Pfam PF00400: WD domain, G-beta
repeat; similar to BEIGE (GI:3928547) [Rattus
norvegicus]; lysosomal trafficking regulator - Bos
taurus, EMBL: AF114785
Length = 3471
Score = 30.3 bits (65), Expect = 1.6
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = -2
Query: 770 SSASVMILIPYLIGERNRFKLLNVFSFLADKDIDVV 663
S+ V +++P+LI + +R +L +FS L DI V
Sbjct: 714 SANGVTVILPFLIADEHRTSILRIFSCLITGDIKQV 749
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 28.3 bits (60), Expect = 6.4
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 217 ILHICTPAVLPTLAKAGWTLALSRAFLSDSDVIG 116
IL +C P +PTLA +T +L+DS +G
Sbjct: 113 ILPVCIPNRIPTLALTNYTQTGYARYLNDSRYVG 146
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,127,814
Number of Sequences: 28952
Number of extensions: 393545
Number of successful extensions: 1177
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 1120
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1170
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1853336000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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