Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0838
(763 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19750.1 68418.m02348 peroxisomal membrane 22 kDa family prot... 89 2e-18
At2g14860.1 68415.m01689 peroxisomal membrane protein 22 kDa, pu... 80 2e-15
At4g33905.1 68417.m04811 peroxisomal membrane protein 22 kDa, pu... 76 2e-14
At5g43140.1 68418.m05266 peroxisomal membrane 22 kDa family prot... 72 5e-13
At1g52870.2 68414.m05978 peroxisomal membrane protein-related co... 71 1e-12
At4g03410.2 68417.m00465 peroxisomal membrane protein-related co... 63 2e-10
At4g03410.1 68417.m00464 peroxisomal membrane protein-related co... 63 2e-10
At2g42770.1 68415.m05296 peroxisomal membrane 22 kDa family prot... 60 1e-09
At3g24570.1 68416.m03087 peroxisomal membrane 22 kDa family prot... 53 2e-07
At1g52870.1 68414.m05977 peroxisomal membrane protein-related co... 50 1e-06
At4g04470.1 68417.m00649 peroxisomal membrane protein 22 kDa (PM... 49 3e-06
At4g14310.1 68417.m02204 peroxisomal membrane protein-related co... 35 0.068
At2g35130.1 68415.m04309 pentatricopeptide (PPR) repeat-containi... 28 5.9
At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative i... 28 7.8
>At5g19750.1 68418.m02348 peroxisomal membrane 22 kDa family protein
similar to SP|P42925 22 kDa peroxisomal membrane protein
{Mus musculus}; contains Pfam profile PF04117: Mpv17 /
PMP22 family
Length = 288
Score = 89.4 bits (212), Expect = 2e-18
Identities = 53/171 (30%), Positives = 81/171 (47%), Gaps = 2/171 (1%)
Frame = +3
Query: 204 LLAWSRQSLQRRPVLTNTVVYATFYTAAEL-SQQTFNRIYSPEKPDLDVAAAARIVSVGS 380
LL+W + L PVLT V A +L Q T N+ S LD +G
Sbjct: 114 LLSWYQALLSNSPVLTKAVTAALLNLVGDLICQLTINKTSS-----LDKKRTLTFTFLGL 168
Query: 381 TVYAPTLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGILE-RREDVF 557
+ PTL+FWY +L K + + ++ DQF+ PI + F + + LE + +V
Sbjct: 169 GLVGPTLHFWYLYLSKVVTASGLSGAVIRLLLDQFVFAPIFVGVFLSAVVTLEGKPSNVI 228
Query: 558 EELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCF 710
+L+Q++ IAN WIP Q +NF F+P + +V+ + W IL F
Sbjct: 229 PKLQQEWTGAMIANWQLWIPFQFLNFRFVPQNYQVLASNVVALAWNVILSF 279
>At2g14860.1 68415.m01689 peroxisomal membrane protein 22 kDa,
putative similar to 22 kDa peroxisomal membrane protein
{Mus musculus} SWISS-PROT:P42925, NCBI_gi:454833
Length = 252
Score = 79.8 bits (188), Expect = 2e-15
Identities = 48/175 (27%), Positives = 79/175 (45%), Gaps = 2/175 (1%)
Frame = +3
Query: 207 LAWSRQSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKPDLDVAAAARIVSVGSTV 386
+ W ++ PV+T +V + Y AA+LS QT + S D+ AR+ G V
Sbjct: 74 VGWYLGMVKSHPVVTKSVTSSLIYIAADLSSQTIAKTSSES---YDLVRTARMGGYGLFV 130
Query: 387 YAPTLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERREDVFE 560
PTL++W+ F+ + F + K+A Q I PI+ F++L L ER +
Sbjct: 131 LGPTLHYWFNFMSRLFPKQDLITTFKKMAMGQTIYGPIMTVIFFSLNASLQGERGSVILA 190
Query: 561 ELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQK 725
LK+ +W I F F P HL+ + S S+VW + ++ ++
Sbjct: 191 RLKRDLLPALFNGVMYWPLCDFITFRFFPVHLQPLVSNSFSYVWTIYMTYMANRE 245
>At4g33905.1 68417.m04811 peroxisomal membrane protein 22 kDa,
putative similar to 22 kDa peroxisomal membrane protein
PMP22 [Mus musculus] gi|10954089|gb|AAG25724
Length = 261
Score = 76.2 bits (179), Expect = 2e-14
Identities = 45/182 (24%), Positives = 80/182 (43%), Gaps = 2/182 (1%)
Frame = +3
Query: 189 IMASKLLAWSRQSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKPDLDVAAAARIV 368
+ + + W ++ RPVLT +V + Y AA+LS QT I D+ AR+
Sbjct: 77 VSTAGFIGWYLGMVKSRPVLTKSVTSSLIYIAADLSSQT---IPQASVDSYDLVRTARMG 133
Query: 369 SVGSTVYAPTLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ER 542
G + PTL++W+ + F + K+A Q + P + F++L L E
Sbjct: 134 GYGLLILGPTLHYWFNLMSSLFPKRDLITTFKKMAMGQTVYGPAMNVVFFSLNAALQGEN 193
Query: 543 REDVFEELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQ 722
++ LK+ T + +W I F F P +L+ + S S++W + ++ +
Sbjct: 194 GSEIVARLKRDLLPTMLNGVMYWPLCDFITFKFCPVYLQPLVSNSFSYLWTIYITYMASR 253
Query: 723 KT 728
T
Sbjct: 254 AT 255
>At5g43140.1 68418.m05266 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 254
Score = 71.7 bits (168), Expect = 5e-13
Identities = 40/174 (22%), Positives = 74/174 (42%), Gaps = 2/174 (1%)
Frame = +3
Query: 207 LAWSRQSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKPDLDVAAAARIVSVGSTV 386
L W + L+ P +T ++ + Y AA+L+ Q I D+ AR+ S G
Sbjct: 79 LRWYLRKLESHPFMTKSITTSVIYMAADLTSQM---ITMEPTGSFDLIRTARMASFGLIF 135
Query: 387 YAPTLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERREDVFE 560
P+ + W+ +L K + K+ Q + P+ FY+ L E E++
Sbjct: 136 LGPSQHLWFSYLSKILPKRDVLTTFKKIMMGQVLFGPVSNTVFYSYNAALQGENSEEIVA 195
Query: 561 ELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQ 722
LK+ T +W + F ++P HL+ + +S +++W L ++ Q
Sbjct: 196 RLKRDLLPTLKNGLMYWPVCDFVTFKYVPVHLQPLMNSSCAYIWTIYLTYMANQ 249
>At1g52870.2 68414.m05978 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:Q07066 22 kDa
peroxisomal membrane protein [Rattus norvegicus]
Length = 366
Score = 70.5 bits (165), Expect = 1e-12
Identities = 46/173 (26%), Positives = 81/173 (46%), Gaps = 4/173 (2%)
Frame = +3
Query: 222 QSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKP--DLDVAAAARIVSVGSTVYAP 395
++L++ PVL V+ Y+ + Q + KP ++D A R VG T++
Sbjct: 175 EALKQNPVLAKMVISGVVYSVGDWIAQCYEG-----KPLFEIDRARTLRSGLVGFTLHGS 229
Query: 396 TLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERREDVFEELK 569
+F+Y+F ++ F V KVA DQ + + I + ++T+LG L E +F+ELK
Sbjct: 230 LSHFYYQFCEELFPFQDWWVVPVKVAFDQTVWSAIWNSIYFTVLGFLRFESPISIFKELK 289
Query: 570 QKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQKT 728
+ A W A I + +P R+++V +W+ IL +K+
Sbjct: 290 ATFLPMLTAGWKLWPFAHLITYGLVPVEQRLLWVDCVELIWVTILSTYSNEKS 342
>At4g03410.2 68417.m00465 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:P42925 22 kDa
peroxisomal membrane protein [Mus musculus]
Length = 361
Score = 63.3 bits (147), Expect = 2e-10
Identities = 42/172 (24%), Positives = 75/172 (43%), Gaps = 4/172 (2%)
Frame = +3
Query: 222 QSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKP--DLDVAAAARIVSVGSTVYAP 395
Q L+ PVL + Y+ + Q + KP + D R VG T++
Sbjct: 133 QILKTNPVLAKMAISGIVYSLGDWIAQCYEG-----KPLFEFDRTRVLRSGLVGFTLHGS 187
Query: 396 TLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERREDVFEELK 569
+++Y+F + F V KVA DQ + + I + ++T+LG+L + D+F E+K
Sbjct: 188 LSHYYYQFCEALFPFQEWWVVPAKVAFDQTVWSAIWNSIYFTVLGLLRFQSPADIFSEIK 247
Query: 570 QKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQK 725
+ A W A + + +P R+++V +W+ IL +K
Sbjct: 248 TTFLPMLTAGWKLWPLAHLVTYGVIPVDQRLLWVDCIELIWVTILSTYSNEK 299
>At4g03410.1 68417.m00464 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:P42925 22 kDa
peroxisomal membrane protein [Mus musculus]
Length = 317
Score = 63.3 bits (147), Expect = 2e-10
Identities = 42/172 (24%), Positives = 75/172 (43%), Gaps = 4/172 (2%)
Frame = +3
Query: 222 QSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKP--DLDVAAAARIVSVGSTVYAP 395
Q L+ PVL + Y+ + Q + KP + D R VG T++
Sbjct: 133 QILKTNPVLAKMAISGIVYSLGDWIAQCYEG-----KPLFEFDRTRVLRSGLVGFTLHGS 187
Query: 396 TLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERREDVFEELK 569
+++Y+F + F V KVA DQ + + I + ++T+LG+L + D+F E+K
Sbjct: 188 LSHYYYQFCEALFPFQEWWVVPAKVAFDQTVWSAIWNSIYFTVLGLLRFQSPADIFSEIK 247
Query: 570 QKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQK 725
+ A W A + + +P R+++V +W+ IL +K
Sbjct: 248 TTFLPMLTAGWKLWPLAHLVTYGVIPVDQRLLWVDCIELIWVTILSTYSNEK 299
>At2g42770.1 68415.m05296 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 232
Score = 60.5 bits (140), Expect = 1e-09
Identities = 31/118 (26%), Positives = 55/118 (46%), Gaps = 1/118 (0%)
Frame = +3
Query: 342 DVAAAARIVSVGSTVYAPTLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYT 521
D A R+ S G +Y P Y WY+FLD + KV +Q I+ P ++A +
Sbjct: 105 DWIRALRMSSYGFLLYGPGSYAWYQFLDHSLPKPTATNLVLKVLLNQVILGPSVIAVIFA 164
Query: 522 LLGI-LERREDVFEELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVW 692
+ L + ++ + ++ T + FW+P +NF+ +P RV +++ S W
Sbjct: 165 WNNLWLGKLSELGNKYQKDALPTLLYGFRFWVPVSILNFWVVPLQARVAFMSMGSVFW 222
>At3g24570.1 68416.m03087 peroxisomal membrane 22 kDa family protein
contains Mpv17 / PMP22 family domain, Pfam:PF04117
Length = 235
Score = 52.8 bits (121), Expect = 2e-07
Identities = 34/119 (28%), Positives = 56/119 (47%), Gaps = 8/119 (6%)
Frame = +3
Query: 393 PTLYFWYKFLDK------KFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERRE 548
P +FWY+ LDK ++ + + VA KVA D I P+ L F+T +G +
Sbjct: 93 PVGHFWYEGLDKFIKLKLRYVPKSTRFVAAKVAMDGLIFGPVDLLVFFTYMGFATGKNTA 152
Query: 549 DVFEELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQK 725
+V E LK+ + W Q NF ++P +++YV V L ++++QK
Sbjct: 153 EVKEGLKRDFLPALALEGGAWPLLQIANFRYVPVQYQLLYVNIFCLVDSAFLSWVEQQK 211
>At1g52870.1 68414.m05977 peroxisomal membrane protein-related
contains weak similarity to Swiss-Prot:Q07066 22 kDa
peroxisomal membrane protein [Rattus norvegicus]
Length = 306
Score = 50.4 bits (115), Expect = 1e-06
Identities = 35/123 (28%), Positives = 61/123 (49%), Gaps = 4/123 (3%)
Frame = +3
Query: 222 QSLQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKP--DLDVAAAARIVSVGSTVYAP 395
++L++ PVL V+ Y+ + Q + KP ++D A R VG T++
Sbjct: 175 EALKQNPVLAKMVISGVVYSVGDWIAQCYEG-----KPLFEIDRARTLRSGLVGFTLHGS 229
Query: 396 TLYFWYKFLDKKFAGNAIKAVATKVASDQFIMTPILLATFYTLLGIL--ERREDVFEELK 569
+F+Y+F ++ F V KVA DQ + + I + ++T+LG L E +F+ELK
Sbjct: 230 LSHFYYQFCEELFPFQDWWVVPVKVAFDQTVWSAIWNSIYFTVLGFLRFESPISIFKELK 289
Query: 570 QKY 578
+
Sbjct: 290 ATF 292
>At4g04470.1 68417.m00649 peroxisomal membrane protein 22 kDa
(PMP22) identical to peroxisomal membrane protein
[Arabidopsis thaliana] gi|3980254|emb|CAA06834
Length = 190
Score = 49.2 bits (112), Expect = 3e-06
Identities = 41/171 (23%), Positives = 74/171 (43%), Gaps = 4/171 (2%)
Frame = +3
Query: 228 LQRRPVLTNTVVYATFYTAAELSQQTFNRIYSPEKPDLDVAAAARIVSVGSTVYAPTLYF 407
LQ+ P+ T + +++ Q + I +K L +++ G + P +F
Sbjct: 18 LQQHPLRTKAITAGVLSGVSDVVSQKLSGI---QKIQLR-RVLLKVIFAGGFL-GPAGHF 72
Query: 408 WYKFLDKKFAGNA-IKAVATKVASDQFIMTPILLATFYTLLGILERRED---VFEELKQK 575
++ +LDK F G + VA KV +Q ++P+ F G++ R V E +K+
Sbjct: 73 FHTYLDKFFKGKKDTQTVAKKVILEQLTLSPLNHLLFMIYYGVVIERTPWTLVRERIKKT 132
Query: 576 YWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQKT 728
Y + F+ IN+ ++P H RV+ + +F W L R T
Sbjct: 133 YPTVQLTAWTFFPVVGWINYKYVPLHFRVILHSLVAFFWGIFLTLRARSMT 183
>At4g14310.1 68417.m02204 peroxisomal membrane protein-related
contains weak similarity to Peroxisomal membrane protein
2 (22 kDa peroxisomal membrane protein)
(Swiss-Prot:P42925) [Mus musculus]
Length = 1087
Score = 34.7 bits (76), Expect = 0.068
Identities = 29/119 (24%), Positives = 50/119 (42%), Gaps = 4/119 (3%)
Frame = +3
Query: 393 PTLYFWYKFLDKKFAGN-AIKAVATKVASDQFIMTP---ILLATFYTLLGILERREDVFE 560
P +F++K +D F G VA KV +Q +P L ++Y L+ + V
Sbjct: 968 PFGHFFHKLMDTIFKGKKGNSTVAKKVLLEQLTSSPWNNFLFMSYYGLVVEGRPWKLVKH 1027
Query: 561 ELKQKYWKTFIANQAFWIPAQTINFYFMPSHLRVVYVASTSFVWINILCFIKRQKT*KN 737
+L + Y + FW +N+ ++P RV++ + + W L R KN
Sbjct: 1028 KLGKDYPTIQLTAWKFWPIVGWVNYQYVPLQFRVLFSSFVASCWSIFLNLKARSPVIKN 1086
>At2g35130.1 68415.m04309 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 591
Score = 28.3 bits (60), Expect = 5.9
Identities = 14/33 (42%), Positives = 20/33 (60%)
Frame = +3
Query: 486 IMTPILLATFYTLLGILERREDVFEELKQKYWK 584
I T +L Y G LER E++F ELK+K ++
Sbjct: 474 ISTYNILINIYGKAGFLERIEELFVELKEKNFR 506
>At1g60850.3 68414.m06850 DNA-directed RNA polymerase, putative
identical to RNA polymerase subunit [Arabidopsis
thaliana] GI:514322; contains Pfam profile PF01000: RNA
polymerase Rpb3/RpoA insert domain
Length = 302
Score = 27.9 bits (59), Expect = 7.8
Identities = 20/78 (25%), Positives = 33/78 (42%)
Frame = +3
Query: 480 QFIMTPILLATFYTLLGILERREDVFEELKQKYWKTFIANQAFWIPAQTINFYFMPSHLR 659
+F PI L+ L +++ EL+ K A W P T +Y M +R
Sbjct: 219 EFANNPITPCDLDILIAKLAPGQEI--ELEAHAVKGIGKTHAKWSPVGTA-WYRMHPEVR 275
Query: 660 VVYVASTSFVWINILCFI 713
+Y+ + +W LCF+
Sbjct: 276 SIYINLSYALWETALCFV 293
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,083,008
Number of Sequences: 28952
Number of extensions: 328604
Number of successful extensions: 793
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 782
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1702303248
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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