BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0837 (615 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha... 41 7e-04 At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha... 40 0.001 At2g44370.1 68415.m05519 DC1 domain-containing protein highly si... 31 0.80 >At5g08300.1 68418.m00977 succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial, putative / succinyl-CoA synthetase, alpha chain, putative / SCS-alpha, putative identical to SP|P53586 Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) {Arabidopsis thaliana}; strong similarity to SP|P13086 Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor {Rattus norvegicus}; contains Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding domain Length = 347 Score = 40.7 bits (91), Expect = 7e-04 Identities = 19/34 (55%), Positives = 24/34 (70%) Frame = -2 Query: 584 SGGKGGAMDKIKALEKANVIVTRSPAKMGVELHK 483 SGGKG A DKIK+L A V V SPAK+G +++ Sbjct: 304 SGGKGTAQDKIKSLNDAGVKVVESPAKIGSAMYE 337 >At5g23250.1 68418.m02720 succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial, putative / succinyl-CoA synthetase, alpha chain, putative / SCS-alpha, putative similar to SP|P36967 Succinyl-CoA ligase [GDP-forming] alpha-chain, mitochondrial precursor (EC 6.2.1.4) (Succinyl-CoA synthetase, alpha chain) (SCS-alpha) {Dictyostelium discoideum}; contains Pfam profiles PF00549: CoA-ligase, PF02629: CoA binding domain Length = 341 Score = 40.3 bits (90), Expect = 0.001 Identities = 19/32 (59%), Positives = 22/32 (68%) Frame = -2 Query: 584 SGGKGGAMDKIKALEKANVIVTRSPAKMGVEL 489 SGGKG A DKIK+L A V V SPAK+G + Sbjct: 299 SGGKGTAQDKIKSLRDAGVKVVESPAKIGAAM 330 >At2g44370.1 68415.m05519 DC1 domain-containing protein highly similar to GP|2435515|AF024504 Length = 250 Score = 30.7 bits (66), Expect = 0.80 Identities = 16/42 (38%), Positives = 25/42 (59%) Frame = -2 Query: 587 HSGGKGGAMDKIKALEKANVIVTRSPAKMGVELHKEMKRLEI 462 + GG+G A ++ A+ +A R KM +ELH EM+R +I Sbjct: 201 NKGGRGSAASELAAMLEAQ----REMEKMQIELHMEMQRAKI 238 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,418,131 Number of Sequences: 28952 Number of extensions: 215950 Number of successful extensions: 370 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 361 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 370 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1236350304 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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