Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0829
(727 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR... 31 1.0
At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR... 31 1.0
At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR... 31 1.0
At1g63700.1 68414.m07209 protein kinase, putative contains prote... 30 1.4
At1g68460.1 68414.m07821 adenylate isopentenyltransferase 1 / cy... 30 1.8
At1g80600.1 68414.m09457 acetylornithine aminotransferase, mitoc... 29 3.1
At5g19560.1 68418.m02329 hypothetical protein contains Pfam prof... 28 5.5
At4g09670.1 68417.m01588 oxidoreductase family protein similar t... 28 7.2
At5g67350.1 68418.m08493 expressed protein 27 9.6
At3g11325.1 68416.m01377 hypothetical protein 27 9.6
At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearl... 27 9.6
>At1g02840.3 68414.m00246 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 30.7 bits (66), Expect = 1.0
Identities = 31/86 (36%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = +2
Query: 329 NSYSGRRV*IFCYQAREKVREMVRCSRGRRTL*GLLDSHSILCVRRISEGRSRSTNRKRS 508
N++S V + Y +R+ R SRGR S S R +S RSRS +R RS
Sbjct: 183 NAFSNGYVRVREYDSRKDSRSP---SRGR----SYSKSRSRSRGRSVSRSRSRSRSRSRS 235
Query: 509 YIA--GRWSVGRRTRRSPSPNPHYRS 580
A R S + T RSP P RS
Sbjct: 236 PKAKSSRRSPAKSTSRSPGPRSKSRS 261
>At1g02840.2 68414.m00244 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 285
Score = 30.7 bits (66), Expect = 1.0
Identities = 31/86 (36%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = +2
Query: 329 NSYSGRRV*IFCYQAREKVREMVRCSRGRRTL*GLLDSHSILCVRRISEGRSRSTNRKRS 508
N++S V + Y +R+ R SRGR S S R +S RSRS +R RS
Sbjct: 183 NAFSNGYVRVREYDSRKDSRSP---SRGR----SYSKSRSRSRGRSVSRSRSRSRSRSRS 235
Query: 509 YIA--GRWSVGRRTRRSPSPNPHYRS 580
A R S + T RSP P RS
Sbjct: 236 PKAKSSRRSPAKSTSRSPGPRSKSRS 261
>At1g02840.1 68414.m00245 pre-mRNA splicing factor SF2 (SF2) / SR1
protein identical to SP|O22315 Pre-mRNA splicing factor
SF2 (SR1 protein) {Arabidopsis thaliana}
Length = 303
Score = 30.7 bits (66), Expect = 1.0
Identities = 31/86 (36%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = +2
Query: 329 NSYSGRRV*IFCYQAREKVREMVRCSRGRRTL*GLLDSHSILCVRRISEGRSRSTNRKRS 508
N++S V + Y +R+ R SRGR S S R +S RSRS +R RS
Sbjct: 183 NAFSNGYVRVREYDSRKDSRSP---SRGR----SYSKSRSRSRGRSVSRSRSRSRSRSRS 235
Query: 509 YIA--GRWSVGRRTRRSPSPNPHYRS 580
A R S + T RSP P RS
Sbjct: 236 PKAKSSRRSPAKSTSRSPGPRSKSRS 261
>At1g63700.1 68414.m07209 protein kinase, putative contains protein
kinase domain, Pfam:PF00069; similar to MEK kinase
(MAP3Ka) [Arabidopsis thaliana] gi|4204912|gb|AAD10848
Length = 883
Score = 30.3 bits (65), Expect = 1.4
Identities = 20/53 (37%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +2
Query: 470 SEGRSRSTNRKRSYIAG-RWSVGRRTRRSPSPNPHYRS*TKQHFLQKTELHPL 625
S GRSR + R+R I R ++ R RSPSP+ R Q F +++ PL
Sbjct: 35 SSGRSRKSRRRRDEIVSERGAISRLPSRSPSPST--RVSRCQSFAERSPAVPL 85
>At1g68460.1 68414.m07821 adenylate isopentenyltransferase 1 /
cytokinin synthase (IPT1) identical to adenylate
isopentenyltransferase (IPT1) [Arabidopsis thaliana]
GI:14279054
Length = 357
Score = 29.9 bits (64), Expect = 1.8
Identities = 22/68 (32%), Positives = 32/68 (47%), Gaps = 8/68 (11%)
Frame = +3
Query: 321 GLQIAIPVDEFES--SVIKPEKKFVKWYAA------PAEGEPSRDYWTATQYYVSEESLK 476
G++ AI V EF+ PEKK +KW A A + R+ WT + V + +
Sbjct: 246 GIRKAIGVPEFDGYFKEYPPEKKMIKWDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEML 305
Query: 477 AGAGPQIE 500
AG +IE
Sbjct: 306 KDAGWEIE 313
>At1g80600.1 68414.m09457 acetylornithine aminotransferase,
mitochondrial, putative / acetylornithine transaminase,
putative / AOTA, putative / ACOAT, putative similar to
SP|O04866 Acetylornithine aminotransferase,
mitochondrial precursor (EC 2.6.1.11) (ACOAT)
(Acetylornithine transaminase) (AOTA) {Alnus glutinosa};
contains Pfam profile PF00202: aminotransferase, class
III
Length = 457
Score = 29.1 bits (62), Expect = 3.1
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = +2
Query: 239 GTYSRVKDVLSSGKGC 286
GTY+R VLSSGKGC
Sbjct: 71 GTYARAPVVLSSGKGC 86
>At5g19560.1 68418.m02329 hypothetical protein contains Pfam profile
PF03759: Domain of unknown function (DUF315)
Length = 493
Score = 28.3 bits (60), Expect = 5.5
Identities = 12/29 (41%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = +3
Query: 147 DALADIEYFYSIPRTIKDAENDG-WRRTE 230
D D + F+ +PR ++DA+++G WRR E
Sbjct: 163 DNFKDQKDFWYVPRDMEDADHNGDWRRDE 191
>At4g09670.1 68417.m01588 oxidoreductase family protein similar to
AX110P [Daucus carota] GI:285739; contains Pfam profiles
PF01408: Oxidoreductase family NAD-binding Rossmann
fold, PF02894: Oxidoreductase family C-terminal
alpha/beta domain
Length = 362
Score = 27.9 bits (59), Expect = 7.2
Identities = 15/52 (28%), Positives = 24/52 (46%)
Frame = +3
Query: 501 NGATLQDGGVWVAGPDAHLLRIPTTEAELNSTFFKKQNCIP*HGHTLLLQHD 656
NG + DG +WV P LL+ +++E Q+C G L++D
Sbjct: 121 NGVQIMDGTMWVHNPRTALLKEFLSDSERFGQLKTVQSCFSFAGDEDFLKND 172
>At5g67350.1 68418.m08493 expressed protein
Length = 302
Score = 27.5 bits (58), Expect = 9.6
Identities = 13/34 (38%), Positives = 21/34 (61%), Gaps = 2/34 (5%)
Frame = +2
Query: 461 RRISEGRSRSTNRKRSYIAG--RWSVGRRTRRSP 556
++I G SRS NR+RS++ + SV T+ +P
Sbjct: 215 KKIGSGESRSRNRRRSFMFSDCKCSVSTETKMAP 248
>At3g11325.1 68416.m01377 hypothetical protein
Length = 401
Score = 27.5 bits (58), Expect = 9.6
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +3
Query: 207 NDGWRRTEPPPGPILELRMYCPPGRVVCPLYDTSGFVAGLQI 332
NDG P P L + ++ P G V+ P+ SGF+ + I
Sbjct: 145 NDGRLVKRPTPATALLILIWIPFGMVLSPIRILSGFILPMWI 186
>At1g55860.1 68414.m06406 ubiquitin-protein ligase 1 (UPL1) nearly
identical to ubiquitin-protein ligase 1 [Arabidopsis
thaliana] GI:7108521; E3, HECT-domain protein family;
similar to GI:7108521, GB:AAF36454 from [Arabidopsis
thaliana]
Length = 3891
Score = 27.5 bits (58), Expect = 9.6
Identities = 9/26 (34%), Positives = 16/26 (61%)
Frame = -2
Query: 282 PFPEDSTSLTRE*VPAVVQSCANRHF 205
PFP+D+ + V+++C N+HF
Sbjct: 331 PFPKDAVLQVLRVIRVVLENCTNKHF 356
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,569,702
Number of Sequences: 28952
Number of extensions: 416191
Number of successful extensions: 1108
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 1057
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1105
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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