Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0824
(752 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces pombe... 110 2e-25
SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyce... 45 1e-05
SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein Mam... 36 0.006
SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces po... 30 0.41
SPBC1773.09c |mug184||meiotically upregulated gene Mug184|Schizo... 29 0.71
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 28 1.2
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 1.6
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 27 3.8
SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein 5|... 27 3.8
SPAC23G3.05c |||regulator of G-protein signaling |Schizosaccharo... 26 5.0
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 5.0
SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyc... 26 5.0
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 26 5.0
SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 5.0
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p... 26 6.6
SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.6
SPBC215.15 |sec13||COPII-coated vesicle component Sec13|Schizosa... 26 6.6
SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subu... 26 6.6
SPCC63.11 |prp28||U5 snRNP-associated protein Prp28 |Schizosacch... 26 6.6
SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr 3||... 25 8.8
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 25 8.8
SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces ... 25 8.8
SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 25 8.8
>SPAC3C7.11c |cnx1|cal1, cal1|calnexin |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 560
Score = 110 bits (265), Expect = 2e-25
Identities = 66/167 (39%), Positives = 91/167 (54%), Gaps = 10/167 (5%)
Frame = +1
Query: 220 DKGLKTSEDARFYALSRKF-KPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKD 396
+ GL ++A +A+S +F +P + K LVVQ+ V E+ ++CGG YLK+ + +
Sbjct: 89 EAGLVMKDEAAHHAISYEFDEPINEPEKDLVVQYEVNPEEGLNCGGAYLKLL-AEPTHGE 147
Query: 397 MHGETPYEIMFGPDICGPGTKKVHVIFSYKG-------KNHLIKKDIRCKDDVYTHLYTL 555
M Y IMFGPD CG + VH IF +K + HL + T+LYTL
Sbjct: 148 MSNSIDYRIMFGPDKCGVNDR-VHFIFKHKNPLTGEYSEKHLDSRPASLLKPGITNLYTL 206
Query: 556 IVKPDNTYEVLIDNEKVESGDLEADW--DFLPPKKIKDPEAKKPEDW 690
IVKPD T+EV I+ + V G L D+ LPP +I DPE KP DW
Sbjct: 207 IVKPDQTFEVRINGDVVRQGSLFYDFIPPVLPPVEIYDPEDIKPADW 253
Score = 35.1 bits (77), Expect = 0.011
Identities = 13/21 (61%), Positives = 15/21 (71%)
Frame = +1
Query: 628 DWDFLPPKKIKDPEAKKPEDW 690
DWD P+ I DP+A KPEDW
Sbjct: 269 DWDEDAPRMIPDPDAVKPEDW 289
Score = 31.5 bits (68), Expect = 0.13
Identities = 13/21 (61%), Positives = 14/21 (66%)
Frame = +1
Query: 628 DWDFLPPKKIKDPEAKKPEDW 690
DW P I DPEA+KPEDW
Sbjct: 288 DWLEDEPLYIPDPEAQKPEDW 308
>SPBP4H10.19c |||calreticulin/calnexin homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 381
Score = 44.8 bits (101), Expect = 1e-05
Identities = 52/194 (26%), Positives = 82/194 (42%), Gaps = 8/194 (4%)
Frame = +1
Query: 100 FEEKFPDD---SWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL-S 267
F E+F + +W S W K+ G + L S D+ GL T + + + L S
Sbjct: 33 FSEQFTSEDITTWRSRW--RAPVNKDLGVWDLVEAPG-SHLRDEYGLITLKSNKPHILIS 89
Query: 268 RKFKPFSNEGK--PLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDI 441
P + + P+V+ F VK + CG Y+ + + K++ E P I FG
Sbjct: 90 NLENPTTRQSSSVPIVLSFQVKPTKPWTCGHAYVSLVH-QSNPKNVSKEPPSVIRFGVKK 148
Query: 442 CGPGTKKVHVIFSYKGK--NHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 615
CG I SY GK HL + T +YTL+++ + T + D V +G
Sbjct: 149 CGMFDYISLSIISYDGKVSCHLYDAPPSGLVEGRTSMYTLLLQ-NTTVVIRRDQSVVYTG 207
Query: 616 DLEADWDFLPPKKI 657
D+ ++ P K I
Sbjct: 208 DVGTNFFHSPTKWI 221
>SPAP11E10.02c |mam3|SPAPB1A10.01c|cell agglutination protein
Mam3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1082
Score = 35.9 bits (79), Expect = 0.006
Identities = 26/72 (36%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
Frame = -1
Query: 254 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHE-SSGNFSSKNTSQFIE 78
N SSE F S+S SL P VN + S + ++ L S + S+ NF+S ++S +
Sbjct: 475 NYTSSEAF---STSSSLVVIPPVNSSLVTSSTSFTKFSSLSSSQLSTENFTSASSSLSLT 531
Query: 77 DNASKLTTTSTT 42
+ S L+T STT
Sbjct: 532 NAKSSLSTPSTT 543
>SPBC646.08c |||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 516
Score = 29.9 bits (64), Expect = 0.41
Identities = 13/35 (37%), Positives = 21/35 (60%)
Frame = +2
Query: 626 PIGTSFRPRKSRTLKPRNQKTGMTSPLFQTPKTRS 730
P+G S+RP+ SRT ++ + MT +T K +S
Sbjct: 168 PLGKSYRPKASRTTSSQSVASTMTKSSTKTSKKKS 202
>SPBC1773.09c |mug184||meiotically upregulated gene
Mug184|Schizosaccharomyces pombe|chr 2|||Manual
Length = 551
Score = 29.1 bits (62), Expect = 0.71
Identities = 19/71 (26%), Positives = 34/71 (47%), Gaps = 1/71 (1%)
Frame = +1
Query: 478 SYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLE-ADWDFLPPKK 654
S + K H KK +D Y+H+Y + P + + + ++ ES E + D+LP
Sbjct: 137 STRKKEHFNKKPSFPRDTEYSHIYNMKYDPRSGIGIRVKRQEPESLKKENNNSDYLPKSA 196
Query: 655 IKDPEAKKPED 687
+K + P+D
Sbjct: 197 MKQKKG-GPKD 206
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 28.3 bits (60), Expect = 1.2
Identities = 15/44 (34%), Positives = 19/44 (43%)
Frame = -1
Query: 185 NLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTT 54
N N F + + S +SGNFS T Q DN S +T
Sbjct: 575 NSNSQRPFSTVPSESNVFSRNASGNFSMSQTHQPTTDNTSSFST 618
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 27.9 bits (59), Expect = 1.6
Identities = 17/52 (32%), Positives = 25/52 (48%)
Frame = +2
Query: 566 PTTPMKSSLTMRKLNLAT*RPIGTSFRPRKSRTLKPRNQKTGMTSPLFQTPK 721
P+ P SS +LA+ P ++FRP+ S T+ + TSPL K
Sbjct: 314 PSYPYSSSRPSASASLAS-SPTRSAFRPKTSETVSSEVVSSPPTSPLHSPVK 364
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 26.6 bits (56), Expect = 3.8
Identities = 33/137 (24%), Positives = 59/137 (43%)
Frame = -1
Query: 446 PQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLR 267
P S I S SP S SS S + S S + + + T+ + + + R
Sbjct: 336 PTSSSSTISSSSSSPSSSSFSSTTSSS---KSSSSFSSTVSSSSSTSSSTLTSSSSSSSR 392
Query: 266 ERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQ 87
+ + SS + SSS S + V+ F ++ S ++ SH SS + SS ++
Sbjct: 393 PASSSSHSSSLSSHKSSSSSKSSSAPVSSAFYHN----STSSRSSSHSSSHSLSSLSSKP 448
Query: 86 FIEDNASKLTTTSTTAF 36
+ ++S L T+S+ +
Sbjct: 449 ILTASSSSLLTSSSHTY 465
>SPCC1259.14c |meu27||S. pombe specific UPF0300 family protein
5|Schizosaccharomyces pombe|chr 3|||Manual
Length = 736
Score = 26.6 bits (56), Expect = 3.8
Identities = 18/71 (25%), Positives = 32/71 (45%), Gaps = 6/71 (8%)
Frame = +1
Query: 349 GGGYLKVFDCKLEQKDMHGETPYEIMFGPDICGPGTK----KVHVIFSYKGKNHLIKKDI 516
G +LKV + E M ETP +I++ + G+ +H K + KK++
Sbjct: 614 GKKFLKVDEATREDMLMSAETPDDILWITTLISTGSSMSHFPLHAYLEAKERQEFTKKNL 673
Query: 517 R--CKDDVYTH 543
CK+D + +
Sbjct: 674 LLFCKEDEFLY 684
>SPAC23G3.05c |||regulator of G-protein signaling
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 343
Score = 26.2 bits (55), Expect = 5.0
Identities = 17/69 (24%), Positives = 33/69 (47%)
Frame = +1
Query: 361 LKVFDCKLEQKDMHGETPYEIMFGPDICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYT 540
+K+F + +K + +P+E+M P + P K H + ++R +DV T
Sbjct: 154 VKMFTQIIIEKYFNPASPHEVMLPPQLVQP---------ILDCKEHQRQDELRLFEDVET 204
Query: 541 HLYTLIVKP 567
+L ++KP
Sbjct: 205 YLLNFLLKP 213
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 5.0
Identities = 10/32 (31%), Positives = 18/32 (56%)
Frame = -1
Query: 254 NLASSEVFKPLSSSGSLKNFPAVNLNFPNSFP 159
N +E F+P + SL N P +N ++ ++ P
Sbjct: 685 NSTPNEAFQPFDQTSSLYNVPGLNKSYVSNQP 716
>SPAC31G5.19 |||ATPase with bromodomain protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1190
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +1
Query: 106 EKFPDDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDA 249
+ F +D + +W EH K ++ + + FS+ DD+ + SE +
Sbjct: 49 QAFQEDEGDEDWEEEEHKPKAKRRYNTRSNESFSE-GDDEPFEVSESS 95
>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
Snf21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1199
Score = 26.2 bits (55), Expect = 5.0
Identities = 20/81 (24%), Positives = 39/81 (48%), Gaps = 3/81 (3%)
Frame = -3
Query: 471 YVYLLGSWTTDVRAEHNLIWS---LSVHVLLLQFAVKDLEVSASTVNVLFMFDSELDDQG 301
Y+ L GS D R++ +++ V++ LL L ++ T + + +FDS+ +
Sbjct: 779 YLRLDGSTKADDRSKLLGVFNDPTAEVNLFLLSTRAGGLGLNLQTADTVIIFDSDWNPHQ 838
Query: 300 FTLITERFELTGESIEPRIFR 238
+R G++ E RI+R
Sbjct: 839 DLQAQDRAHRIGQTKEVRIYR 859
>SPAC4H3.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 391
Score = 26.2 bits (55), Expect = 5.0
Identities = 12/28 (42%), Positives = 15/28 (53%)
Frame = +1
Query: 607 ESGDLEADWDFLPPKKIKDPEAKKPEDW 690
E L+ LP + K P AKKPED+
Sbjct: 157 EKNTLDLKHCLLPSPEYKAPPAKKPEDY 184
>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1040
Score = 25.8 bits (54), Expect = 6.6
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 4/58 (6%)
Frame = -1
Query: 278 LNLRERA*NLASSEV----FKPLSSSGSLKNFPAVNLNFPNSFPGCSLYTQLLSHESS 117
++LR RA L+ E+ + LS+ LK +++ FP SFP +Y + ++SS
Sbjct: 121 VDLRMRAIFLSIDEISMICIRILSAEERLKTGRSIDSGFPFSFPVHLIYDMCILNDSS 178
>SPAC11G7.01 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 536
Score = 25.8 bits (54), Expect = 6.6
Identities = 27/123 (21%), Positives = 53/123 (43%), Gaps = 2/123 (1%)
Frame = -1
Query: 386 SSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNLRERA*NLASSEVFKPLSSSG- 210
+SL S P S S + T ++ +PS + + A ++SS PLSSS
Sbjct: 12 TSLSSSASSSIPASSSSAAASTSLSSSSVIPSSSSSMLSSSSATAISSSSSSSPLSSSSF 71
Query: 209 -SLKNFPAVNLNFPNSFPGCSLYTQLLSHESSGNFSSKNTSQFIEDNASKLTTTSTTAFI 33
S + + +S S + L+ SS +S +++ ++S ++S+++ +
Sbjct: 72 TSPASSSFITSLVSSSSQQSSSSSASLTSSSSATLTSSSSASPTSSSSSHALSSSSSSLV 131
Query: 32 FDS 24
S
Sbjct: 132 ASS 134
>SPBC215.15 |sec13||COPII-coated vesicle component
Sec13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 297
Score = 25.8 bits (54), Expect = 6.6
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1
Query: 103 EEKFPDDSWESNWVYS 150
EEKFPD +W +W S
Sbjct: 251 EEKFPDIAWRVSWSLS 266
>SPAC3H5.06c |pol1|swi7, polA|DNA polymerase alpha catalytic subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1405
Score = 25.8 bits (54), Expect = 6.6
Identities = 16/65 (24%), Positives = 29/65 (44%)
Frame = +1
Query: 490 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPE 669
KNH ++ K+ V L + DN Y+ ++ + K + L+ D P +K +
Sbjct: 995 KNHALRIGNEFKEKVNERYSKLEIDIDNVYQRMLLHAKKKYAALQLDSQGKPNLDVKGLD 1054
Query: 670 AKKPE 684
K+ E
Sbjct: 1055 MKRRE 1059
>SPCC63.11 |prp28||U5 snRNP-associated protein Prp28
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 662
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +1
Query: 628 DWDFLPPKKIKDPEAKKPEDW 690
DWD +P ++ K EA KP DW
Sbjct: 209 DWDDVPWRE-KPLEAMKPRDW 228
>SPCC1672.11c |||P-type ATPase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 1315
Score = 25.4 bits (53), Expect = 8.8
Identities = 12/31 (38%), Positives = 14/31 (45%)
Frame = -2
Query: 148 CTPNCFPTNHQETSPQRIHHNLSKIMPAS*Q 56
C P FP N+QE H+ I AS Q
Sbjct: 791 CKPESFPANYQEVLDYYTHNGFRVIACASKQ 821
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 25.4 bits (53), Expect = 8.8
Identities = 18/58 (31%), Positives = 23/58 (39%)
Frame = +1
Query: 232 KTSEDARFYALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHG 405
KT Y +SRK P L + F K DC G L+V D L+ +G
Sbjct: 952 KTIPRDLLYEVSRKINPLHQASTRLAIAFPDKRLLQYDC--GKLQVLDRLLKDLVSNG 1007
>SPCC1020.02 |spc7||kinetochore protein Spc7|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1364
Score = 25.4 bits (53), Expect = 8.8
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +1
Query: 190 AGKFFSDPEDDKGLKTSEDARFYALSRKFKPFSNE 294
A F S E KGL +SE Y+L P SN+
Sbjct: 263 ADSFLSHSESIKGLSSSEQGTVYSLKASHDP-SNQ 296
>SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual
Length = 1236
Score = 25.4 bits (53), Expect = 8.8
Identities = 30/145 (20%), Positives = 58/145 (40%), Gaps = 1/145 (0%)
Frame = -1
Query: 455 VPGPQMSGPNIISYGVSPCMSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGL 276
+P S P+ S SP S SS S S S + ++ ++ +PS + +
Sbjct: 518 IPSTSSSTPSS-SIISSPMTSVLSSSSSIPTSSSSDFSSSITTISSGISSSSIPSTFSSV 576
Query: 275 NLRERA*NLASSEVFKPLSSSGSLKNFPAVNLNFPNSF-PGCSLYTQLLSHESSGNFSSK 99
+ + + S +SSS + F + + + P+S S + +LS + S
Sbjct: 577 SSILSSSTSSPSSTSLSISSSSTSSTFSSASTSSPSSISSSISSSSTILSSPTPSTSSLM 636
Query: 98 NTSQFIEDNASKLTTTSTTAFIFDS 24
+S I +S + ++S + S
Sbjct: 637 ISSSSIISGSSSILSSSISTIPISS 661
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,317,708
Number of Sequences: 5004
Number of extensions: 75955
Number of successful extensions: 273
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 269
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 359287726
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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