Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0824
(752 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 256 1e-68
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 252 2e-67
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 232 2e-61
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 120 1e-27
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 113 1e-25
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 109 1e-24
At5g56820.1 68418.m07090 F-box family protein contains F-box dom... 33 0.15
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 31 0.82
At3g31540.1 68416.m04025 hypothetical protein 31 1.1
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 30 1.4
At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705... 30 1.9
At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kina... 29 3.3
At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like fami... 29 4.4
At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5)... 29 4.4
At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421... 28 5.8
At2g36350.1 68415.m04461 protein kinase, putative similar to pro... 28 5.8
At1g26370.1 68414.m03217 RNA helicase, putative similar to SP|Q1... 28 5.8
At3g14670.1 68416.m01856 hypothetical protein 28 7.7
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 256 bits (627), Expect = 1e-68
Identities = 117/201 (58%), Positives = 147/201 (73%), Gaps = 2/201 (0%)
Frame = +1
Query: 94 VFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYALS 267
V FEE+F DD WE+ WV SE + G++K TAG + D +DKG++TSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRFYAIS 81
Query: 268 RKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDICG 447
+F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++QK G+TPY IMFGPDICG
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICG 141
Query: 448 PGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEA 627
TKKVH I +Y NHLIKKD+ C+ D TH+YT I++PD TY +LIDN + ++G L +
Sbjct: 142 YSTKKVHAILTYNEANHLIKKDVPCETDQLTHVYTFILRPDATYSILIDNVEKQTGSLYS 201
Query: 628 DWDFLPPKKIKDPEAKKPEDW 690
DWD LPPKKIKDP AKKPEDW
Sbjct: 202 DWDLLPPKKIKDPSAKKPEDW 222
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 252 bits (617), Expect = 2e-67
Identities = 113/205 (55%), Positives = 148/205 (72%), Gaps = 2/205 (0%)
Frame = +1
Query: 82 INCDVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARF 255
++ +V FEEKF +D WE WV S+ + G++K TAG + D +DKG++TSED RF
Sbjct: 20 VSAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDA-NDKGIQTSEDYRF 77
Query: 256 YALSRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGP 435
YA+S +F FSN+ K LV QF+VKHEQ +DCGGGY+K+ ++Q G+TPY IMFGP
Sbjct: 78 YAISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTPYSIMFGP 137
Query: 436 DICGPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESG 615
DICG TKKVH I +Y G NHLIKK++ C+ D TH+YT +++PD TY +LIDN + ++G
Sbjct: 138 DICGYSTKKVHAILTYNGTNHLIKKEVPCETDQLTHVYTFVLRPDATYSILIDNVEKQTG 197
Query: 616 DLEADWDFLPPKKIKDPEAKKPEDW 690
L +DWD LP KKIKDP AKKPEDW
Sbjct: 198 SLYSDWDLLPAKKIKDPSAKKPEDW 222
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 232 bits (568), Expect = 2e-61
Identities = 106/202 (52%), Positives = 142/202 (70%), Gaps = 2/202 (0%)
Frame = +1
Query: 91 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 264
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 265 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDIC 444
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY +MFGPDIC
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSLMFGPDIC 146
Query: 445 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLE 624
G TKK+HVI SY+G+N+ IKKD++C+ D H YT I++PD +Y VL+DN++ E G +
Sbjct: 147 GTQTKKLHVIVSYQGQNYPIKKDLQCETDKLNHFYTFILRPDASYSVLVDNKEREFGSMY 206
Query: 625 ADWDFLPPKKIKDPEAKKPEDW 690
DWD LPP+KIK AKKPEDW
Sbjct: 207 TDWDILPPRKIKVKNAKKPEDW 228
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 120 bits (288), Expect = 1e-27
Identities = 72/205 (35%), Positives = 110/205 (53%), Gaps = 14/205 (6%)
Frame = +1
Query: 118 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF-KPFSNE 294
D+ ++ W+ S++ E G +K S+ +D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 295 GKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGTKKV 465
+V+Q+ V+ ++ ++CGG YLK + + E+PY IMFGPD CG GT KV
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESPYSIMFGPDKCG-GTNKV 149
Query: 466 HVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEA 627
H I +K H +K D +H+YT I+KPDN +L+D E+ + +L +
Sbjct: 150 HFILKHKNPKSGEYVEHHLKFPPSVPYDKLSHVYTAILKPDNEVRILVDGEEKKKANLLS 209
Query: 628 DWDF----LPPKKIKDPEAKKPEDW 690
DF +P K I DPE KKPEDW
Sbjct: 210 GEDFEPALIPAKTIPDPEDKKPEDW 234
Score = 32.7 bits (71), Expect = 0.27
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +1
Query: 559 VKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPEAKKPEDW 690
VKP++ E + E + W P+++ DPEA KPEDW
Sbjct: 246 VKPEDWDEDAPMEIEDEEAEKPEGWLDDEPEEVDDPEATKPEDW 289
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 113 bits (272), Expect = 1e-25
Identities = 73/208 (35%), Positives = 110/208 (52%), Gaps = 17/208 (8%)
Frame = +1
Query: 118 DDSWESNWVYSEHPGKEFGKFKLTAGKFFSDPEDDKGLKTSEDARFYALSRKF---KPFS 288
D+ ++ WV SE + G +K S+ DD GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEK----SEGHDDYGLLVSEKAKKYGIVKELDVDEPLN 90
Query: 289 -NEGKPLVVQFTVKHEQDIDCGGGYLKVF---DCKLEQKDMHGETPYEIMFGPDICGPGT 456
NEG +V+Q+ + ++ ++CGG YLK + + ++PY IMFGPD CG T
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLKYLRPQEAGWVPQGFDNDSPYSIMFGPDKCG-AT 148
Query: 457 KKVHVIFSYKGKN------HLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGD 618
KVH I +K H +K D+ +H+YT ++K DN +L+D E+ + G+
Sbjct: 149 NKVHFILKHKNPKSGEFVEHHLKFPPSVPFDMLSHVYTAVLKSDNEVRILVDGEEKKKGN 208
Query: 619 LEADWDFLPP----KKIKDPEAKKPEDW 690
L + DF PP K I DPE KKPEDW
Sbjct: 209 LLSAEDFEPPLIPSKTIPDPEDKKPEDW 236
Score = 34.3 bits (75), Expect = 0.088
Identities = 17/44 (38%), Positives = 23/44 (52%)
Frame = +1
Query: 559 VKPDNTYEVLIDNEKVESGDLEADWDFLPPKKIKDPEAKKPEDW 690
VKPD+ E + E + W P +++DPEA KPEDW
Sbjct: 248 VKPDDWDEDAPMEIEDEEAEKPEGWLDDEPVEVEDPEASKPEDW 291
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 109 bits (263), Expect = 1e-24
Identities = 72/203 (35%), Positives = 110/203 (54%), Gaps = 3/203 (1%)
Frame = +1
Query: 91 DVFFEEKFPDDSWESNWVYSEHPGKE--FGKFKLTAGKFFSDPEDDKGLKTSEDARFYAL 264
++F EE F + W+S WV S+ E G FK TAGK+ DP D+KG++T DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDP-DNKGIQTYNDAKHYAI 86
Query: 265 SRKFKPFSNEGKPLVVQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEIMFGPDIC 444
S K FSN+ + LVVQ++VK EQDI+CGG Y+K+ + QK G+TPY ++
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTPYSVLV----- 141
Query: 445 GPGTKKVHVIFSYKGKNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVES-GDL 621
K+ Y + L + I+ K+ KP++ D E ++ D+
Sbjct: 142 --DNKEREFGSMYTDWDILPPRKIKVKN---------AKKPED----WDDREYIDDPNDV 186
Query: 622 EADWDFLPPKKIKDPEAKKPEDW 690
+ + P++I D +AK+PEDW
Sbjct: 187 KPEGFDSIPREIPDRKAKEPEDW 209
>At5g56820.1 68418.m07090 F-box family protein contains F-box domain
Pfam:PF00646
Length = 435
Score = 33.5 bits (73), Expect = 0.15
Identities = 28/101 (27%), Positives = 52/101 (51%), Gaps = 6/101 (5%)
Frame = -1
Query: 287 LNG---LNLRERA*NLASSEVF-KPLSSSGSLKNFPAVNLNFPNSFPGC-SLYTQLLSHE 123
LNG L+L + + NL + ++ KPL S +K+ P +++ PNS P C + + + L +
Sbjct: 305 LNGDRILSLLKHSPNLQTLKLNEKPLRS---IKDQPNISVRKPNSVPECLTFHLETLEWQ 361
Query: 122 S-SGNFSSKNTSQFIEDNASKLTTTSTTAFIFDSQYHFFNK 3
+G K + +I NA +L T + + + S++ K
Sbjct: 362 GYAGRPEDKEIAVYILGNALRLNTATISRYFSSSRFRHHQK 402
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 31.1 bits (67), Expect = 0.82
Identities = 12/38 (31%), Positives = 19/38 (50%)
Frame = +1
Query: 310 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYEI 423
++F KH D GG +F L + ++ GE P E+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVEL 867
>At3g31540.1 68416.m04025 hypothetical protein
Length = 699
Score = 30.7 bits (66), Expect = 1.1
Identities = 17/53 (32%), Positives = 23/53 (43%)
Frame = +1
Query: 490 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEADWDFLPP 648
KN I + CKDD YT + + D E L+ + G L+ FL P
Sbjct: 252 KNMKIIEGFPCKDDAYTDYFFFVALEDAVLEDLVGKVLTKWGILDRPIRFLEP 304
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 30.3 bits (65), Expect = 1.4
Identities = 12/37 (32%), Positives = 18/37 (48%)
Frame = +1
Query: 310 VQFTVKHEQDIDCGGGYLKVFDCKLEQKDMHGETPYE 420
++F KH D GG +F L + ++ GE P E
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPVE 798
>At2g15695.1 68415.m01797 expressed protein contains Pfam PF05705:
Eukaryotic protein of unknown function (DUF829)
Length = 420
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/43 (37%), Positives = 22/43 (51%)
Frame = -1
Query: 398 MSFCSSLQSKTLRYPPPQSMSCSCLTVNWTTKGLPSLLNGLNL 270
+ F S L K +P + MS V+W KG+ S L+GL L
Sbjct: 147 LDFTSDLNVKFALHPTIRRMSGPSRLVSWVAKGISSGLDGLYL 189
>At1g60890.1 68414.m06855 phosphatidylinositol-4-phosphate 5-kinase
family protein similar to
phosphatidylinositol-4-phosphate 5-kinase AtPIP5K1
[Arabidopsis thaliana] GI:3702691; contains Pfam
profiles PF01504: Phosphatidylinositol-4-phosphate
5-Kinase, PF02493: MORN repeat
Length = 769
Score = 29.1 bits (62), Expect = 3.3
Identities = 14/30 (46%), Positives = 19/30 (63%)
Frame = -1
Query: 137 LLSHESSGNFSSKNTSQFIEDNASKLTTTS 48
LLSH S+ N TS+ + + S+LTTTS
Sbjct: 218 LLSHNSTINIDDLRTSKAVSRSLSELTTTS 247
>At3g56640.1 68416.m06298 exocyst complex subunit Sec15-like family
protein contains Pfam profile PF04091: Exocyst complex
subunit Sec15-like
Length = 789
Score = 28.7 bits (61), Expect = 4.4
Identities = 18/47 (38%), Positives = 25/47 (53%)
Frame = +1
Query: 490 KNHLIKKDIRCKDDVYTHLYTLIVKPDNTYEVLIDNEKVESGDLEAD 630
KN KK+ +D TH IV D VL+D E+++S DL +D
Sbjct: 53 KNVARKKEAEIEDLCKTHYEEFIVAVDELRGVLVDAEELKS-DLASD 98
>At3g02885.1 68416.m00283 gibberellin-regulated protein 5 (GASA5) /
gibberellin-responsive protein 5 identical to GASA5
[Arabidopsis thaliana] GI:1289320
Length = 97
Score = 28.7 bits (61), Expect = 4.4
Identities = 15/37 (40%), Positives = 16/37 (43%), Gaps = 3/37 (8%)
Frame = -1
Query: 404 PCMSFCSSLQSKTLRYPP---PQSMSCSCLTVNWTTK 303
PCM FC K L PP +C C NW TK
Sbjct: 55 PCMFFCLKCCKKCLCVPPGTFGNKQTCPCYN-NWKTK 90
>At4g16410.1 68417.m02483 expressed protein contains Pfam PF05421:
Protein of unknown function (DUF751)
Length = 185
Score = 28.3 bits (60), Expect = 5.8
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 3/59 (5%)
Frame = +2
Query: 242 KMRGSMLSP---VSSNRSVMRVNPWSSSSLSNMNKTLTVEADTSRSLTANWSRRTCTER 409
KMR S L P +S+RS+ + SSS+ N L R LT SR + ER
Sbjct: 8 KMRLSCLQPHLHFTSSRSIAAALAFPSSSIRNRTSCLVRCGSARRRLTTKSSRNSKLER 66
>At2g36350.1 68415.m04461 protein kinase, putative similar to
protein kinase KIPK (KCBP-interacting protein kinase)
[Arabidopsis thaliana] gi|7716430|gb|AAF68383
Length = 949
Score = 28.3 bits (60), Expect = 5.8
Identities = 18/49 (36%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = +2
Query: 290 MRVNPWSSS----SLSNMNKTLTVEADTSRSLTANWSRRTCTERLHMRL 424
+R NP SS SLS+ N + TS S +N SR +C + HM +
Sbjct: 489 IRANPTSSEKFDFSLSSKNSLGDYSSSTSMSEESNLSRFSCGNKPHMSM 537
>At1g26370.1 68414.m03217 RNA helicase, putative similar to
SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
profiles PF04408: Helicase associated domain (HA2),
PF00271: Helicase conserved C-terminal domain
Length = 717
Score = 28.3 bits (60), Expect = 5.8
Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = -3
Query: 654 FLGRKEVPIGL*V--ARFNFLIVNEDFIGVVGFHNQSVQMCVDIIFAADIFFDEVVLTLV 481
+ GRK P+ L + A + + +E F G H Q Q VDI++ D V TLV
Sbjct: 221 YQGRKLSPLKLIIMSASLDARVFSEYFGGAKAVHVQGRQFPVDILYTVHPESDYVDATLV 280
Query: 480 T 478
T
Sbjct: 281 T 281
>At3g14670.1 68416.m01856 hypothetical protein
Length = 232
Score = 27.9 bits (59), Expect = 7.7
Identities = 13/29 (44%), Positives = 17/29 (58%)
Frame = +1
Query: 601 KVESGDLEADWDFLPPKKIKDPEAKKPED 687
K + GDLE D D K KDP+ K P++
Sbjct: 169 KEKDGDLEKDGDQEKDPKEKDPKEKDPKE 197
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 17,576,685
Number of Sequences: 28952
Number of extensions: 411976
Number of successful extensions: 1221
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 1160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1207
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1672953192
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -