Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0819
(844 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 130 3e-31
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 89 7e-19
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 71 3e-13
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 65 1e-11
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 60 4e-10
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 56 5e-09
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 47 4e-06
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 6e-04
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 38 0.002
SPAC22G7.06c |ura1||carbamoyl-phosphate synthase |Schizosaccharo... 29 0.62
SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr 2|||Ma... 29 0.62
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 28 1.4
SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit Srb9... 27 2.5
SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces... 26 5.8
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 26 7.7
SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain protein|Schizosacchar... 26 7.7
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 130 bits (313), Expect = 3e-31
Identities = 70/178 (39%), Positives = 106/178 (59%), Gaps = 3/178 (1%)
Frame = +2
Query: 113 LSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQ 292
++K +IT + ++V+FYAPWCGHCK+LAPEY AA +L E+ I L +VD T+E
Sbjct: 27 VNKEGLNELITADKVLMVKFYAPWCGHCKALAPEYESAADEL--EKDGISLVEVDCTEEG 84
Query: 293 DLAESYGVRGYPTLKFFRNGSPI-DYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKELI 469
DL Y +RGYPTL F+NG I YSG R+ D ++ +++K+ P V+ S + + +
Sbjct: 85 DLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALVKYMRKQL-LPTVKPISKDTLENFV 143
Query: 470 D-ANTVIVFGFFSDQSSARAKTFLSTAQVV-DDQVFAIVSDEKVIKELEAEDEDVVLF 637
+ A+ + V FF DQ T+ A+V+ DD VFA D+++ K L + +V F
Sbjct: 144 EKADDLAVVAFFKDQK--LNDTYTEVAEVMKDDFVFAASDDKELAKSLGSNFPGIVAF 199
Score = 91.5 bits (217), Expect = 1e-19
Identities = 45/111 (40%), Positives = 75/111 (67%), Gaps = 4/111 (3%)
Frame = +2
Query: 92 TEENVLVLSKANFETVITT-TEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 268
++E+++VL NF+ ++ T+ +LVEFYAPWCGHCK+LAP Y K A + + ++S + +A
Sbjct: 353 SQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYS-DDSNVVVA 411
Query: 269 KVDATQEQDLAESYGVRGYPTLKFFRNG---SPIDYSGGRQADDIISWLKK 412
K+DAT E D+ S + G+PT+ FF+ +P+ Y G R +D+ +++ K
Sbjct: 412 KIDAT-ENDI--SVSISGFPTIMFFKANDKVNPVRYEGDRTLEDLSAFIDK 459
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 89.0 bits (211), Expect = 7e-19
Identities = 50/154 (32%), Positives = 79/154 (51%), Gaps = 2/154 (1%)
Frame = +2
Query: 23 IEMRVLIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKS 202
+ + +L F AL L V +++ L T+ + + L+EFYA WCGHCKS
Sbjct: 1 MRLPLLSFVIFALFALVFASGVVELQSLNELEN----TIRASKKGALIEFYATWCGHCKS 56
Query: 203 LAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF-RNGS-PIDYSGG 376
LAP Y + L E+ + + + K+DA D+A+ Y + G+PTL +F +GS P+ YS
Sbjct: 57 LAPVYEELGA-LFEDHNDVLIGKIDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115
Query: 377 RQADDIISWLKKKTGPPAVEVTSAEQAKELIDAN 478
R D + ++ +KTG ++ EL N
Sbjct: 116 RDVDSLTQFVSEKTGIKKRKIVLPSNVVELDSLN 149
Score = 79.4 bits (187), Expect = 6e-16
Identities = 40/111 (36%), Positives = 63/111 (56%), Gaps = 4/111 (3%)
Frame = +2
Query: 101 NVLVLSKANFETVITTTEY-ILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVD 277
NV+ L NF+ V+ + +LVEFYA WCG+CK LAP Y + K+ + E +++ K++
Sbjct: 141 NVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTY-ETLGKVFKNEPNVEIVKIN 199
Query: 278 ATQEQDLAESYGVRGYPTLKFF---RNGSPIDYSGGRQADDIISWLKKKTG 421
A D+ + V +PT+KFF P Y G R + +I ++ KK+G
Sbjct: 200 ADVFADIGRLHEVASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSG 250
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 70.5 bits (165), Expect = 3e-13
Identities = 43/144 (29%), Positives = 65/144 (45%), Gaps = 6/144 (4%)
Frame = +2
Query: 38 LIFTAIALLGLALGDEVPTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEY 217
L +L+ G N + L+ NF + LV FYAPWCG+CK L P Y
Sbjct: 11 LFLACFSLVSGVFGYSPMFGSNTIELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTY 70
Query: 218 AKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFF---RNGSPI---DYSGGR 379
K A+ L P+ DA Q + + Y V+G+PT+K GS + DY+G R
Sbjct: 71 QKLASNL-HSLLPVTAVDCDADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDR 129
Query: 380 QADDIISWLKKKTGPPAVEVTSAE 451
+ ++ P V++ ++E
Sbjct: 130 SYKSLQKFVSDSI-PSKVKILTSE 152
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 65.3 bits (152), Expect = 1e-11
Identities = 38/121 (31%), Positives = 64/121 (52%), Gaps = 4/121 (3%)
Frame = +2
Query: 110 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 289
+ S ++ + I + Y+ V+ YA WCG CK+++P +++ A+K A + AKV+ ++
Sbjct: 6 IRSYQHWISTIPKSGYLAVDCYADWCGPCKAISPLFSQLASKYASPK--FVFAKVNVDEQ 63
Query: 290 QDLAESYGVRGYPTLKFFRNGSPIDYSGGR--QA--DDIISWLKKKTGPPAVEVTSAEQA 457
+ +A GV+ PT FF NG ID G QA + + K TG A+ +S+
Sbjct: 64 RQIASGLGVKAMPTFVFFENGKQIDMLTGANPQALKEKVALISSKATGTGALASSSSAPV 123
Query: 458 K 460
K
Sbjct: 124 K 124
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 60.1 bits (139), Expect = 4e-10
Identities = 34/101 (33%), Positives = 49/101 (48%)
Frame = +2
Query: 110 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 289
V S ++ T I+ + +V+FYA WCG CK L P KL+E+ V+A +
Sbjct: 22 VESFGDYNTRISADKVTVVDFYADWCGPCKYLKP----FLEKLSEQNQKASFIAVNADKF 77
Query: 290 QDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKK 412
D+A+ GV PT+ FR G +D G + S L K
Sbjct: 78 SDIAQKNGVYALPTMVLFRKGQELDRIVGADVKTLSSLLAK 118
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 56.4 bits (130), Expect = 5e-09
Identities = 24/85 (28%), Positives = 46/85 (54%)
Frame = +2
Query: 110 VLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQE 289
V + F++++ + ++V+F+A WCG CK++AP++ + + S KVD Q
Sbjct: 5 VSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKF----EQFSNTYSDATFIKVDVDQL 60
Query: 290 QDLAESYGVRGYPTLKFFRNGSPID 364
++A GV P+ ++NG I+
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIE 85
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 46.8 bits (106), Expect = 4e-06
Identities = 28/144 (19%), Positives = 63/144 (43%)
Frame = +2
Query: 89 PTEENVLVLSKANFETVITTTEYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLA 268
PT + + A+ + +T E ++FY+ C C ++ + A ++ + +A
Sbjct: 278 PTGTSKALALDADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRM---RGKLNVA 334
Query: 269 KVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVEVTSA 448
++ + + Y ++ +PT FF+ + ++Y G D++S+ ++ EV
Sbjct: 335 HINCAVSKRACKQYSIQYFPTFLFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIREVELL 394
Query: 449 EQAKELIDANTVIVFGFFSDQSSA 520
+ + N + F +F D SA
Sbjct: 395 DTVN--AEKNGDVFFLYFYDDDSA 416
Score = 42.3 bits (95), Expect = 8e-05
Identities = 22/69 (31%), Positives = 35/69 (50%), Gaps = 2/69 (2%)
Frame = +2
Query: 164 VEFYAPWCGHCKSLAPEYAKAATKLAE--EESPIKLAKVDATQEQDLAESYGVRGYPTLK 337
+++Y P CG CK L P + K E E S +VD ++E L+ +R PTL
Sbjct: 47 IKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKE--LSSCANIRAVPTLY 104
Query: 338 FFRNGSPID 364
++NG ++
Sbjct: 105 LYQNGEIVE 113
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 39.5 bits (88), Expect = 6e-04
Identities = 27/123 (21%), Positives = 50/123 (40%)
Frame = +2
Query: 152 EYILVEFYAPWCGHCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPT 331
+ IL+ FYAPW CK + + + A++ K++A + D+AES+ V P
Sbjct: 21 QIILLNFYAPWAAPCKQMNQVF----DQFAKDTKNAVFLKIEAEKFSDIAESFDVNAVPL 76
Query: 332 LKFFRNGSPIDYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGFFSDQ 511
+ G + + + + P +++S + E N+V S+
Sbjct: 77 FVLIHGAKVLARISGANPQKLKAAIDEYIQPLISQISSTNASVE-TQVNSVQTTNTTSNT 135
Query: 512 SSA 520
S A
Sbjct: 136 SKA 138
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 37.5 bits (83), Expect = 0.002
Identities = 15/87 (17%), Positives = 43/87 (49%)
Frame = +2
Query: 191 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 370
HC+ A ++ + +K+A+V+ +E+++ + ++ +PT + F+ I Y+
Sbjct: 208 HCEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYN 267
Query: 371 GGRQADDIISWLKKKTGPPAVEVTSAE 451
G + ++S+ + A+++ +
Sbjct: 268 GPLKYQQLLSYSNQVASYQAIKIEEGD 294
Score = 28.3 bits (60), Expect = 1.4
Identities = 18/54 (33%), Positives = 25/54 (46%), Gaps = 5/54 (9%)
Frame = +2
Query: 296 LAESYGVRGYPTLKFFRNGSPIDYSG--GRQADD---IISWLKKKTGPPAVEVT 442
LA YG + P++ RNG PI Y R+ D I W+ + P E+T
Sbjct: 343 LANKYGAQSQPSIIAVRNGMPIVYQAITPREFRDYKRITEWINIVSSPFITELT 396
>SPAC22G7.06c |ura1||carbamoyl-phosphate synthase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2244
Score = 29.5 bits (63), Expect = 0.62
Identities = 30/124 (24%), Positives = 54/124 (43%), Gaps = 8/124 (6%)
Frame = +2
Query: 179 PWCG-HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGS 355
PW + ++L + + KL E + K D + L G++ Y ++ F N
Sbjct: 226 PWKNPNTENLTSQVSIKEPKLYEPHPTTAIKKADGKIIRILVIDVGMK-YNQIRCFLNRG 284
Query: 356 ------PIDYSGGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGF-FSDQS 514
P DY ++ D + ++ G P++ ++ K ++++ TV VFG F Q
Sbjct: 285 VELLVVPWDYDFTKETYDGL-FISNGPGDPSLMDLVVDRVKRVLESKTVPVFGICFGHQI 343
Query: 515 SARA 526
ARA
Sbjct: 344 MARA 347
>SPBC16G5.02c |||ribokinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 318
Score = 29.5 bits (63), Expect = 0.62
Identities = 27/86 (31%), Positives = 40/86 (46%)
Frame = +2
Query: 191 HCKSLAPEYAKAATKLAEEESPIKLAKVDATQEQDLAESYGVRGYPTLKFFRNGSPIDYS 370
+C L P +AA L + +SP L VDA + L S+GVR + G+ +
Sbjct: 183 YCAYLVPNEHEAAILLNQADSPATLENVDAYASKLL--SFGVRKAVIITLGSQGAYYKSA 240
Query: 371 GGRQADDIISWLKKKTGPPAVEVTSA 448
G A ++S K K AV+ T+A
Sbjct: 241 NGESA--LVSACKVK----AVDTTAA 260
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1778
Score = 28.3 bits (60), Expect = 1.4
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = -2
Query: 537 RKVLALAELWSEKKPNTITVLASISSLACSAEVTSTA 427
+K +L + W + + LASISS A SA + ST+
Sbjct: 673 KKAASLPQFWLSPRSHNTARLASISSFAKSAVMNSTS 709
>SPAC589.02c |med13|spTrap240, srb9|mediator complex subunit
Srb9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1223
Score = 27.5 bits (58), Expect = 2.5
Identities = 14/41 (34%), Positives = 19/41 (46%)
Frame = +3
Query: 99 KMCSF*VKLTLKL*LQPRSTF*LNSMLHGAATANLWHRNTP 221
K +F L LQP T N +L+ + NLW R+ P
Sbjct: 620 KSANFDFSFLKSLDLQPTITLGKNDLLNAILSQNLWFRSLP 660
>SPBP16F5.03c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3699
Score = 26.2 bits (55), Expect = 5.8
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = -2
Query: 282 VASTFASLIGDSSSASFVAALAYSGARDLQW 190
V++ F SL+G S FVA + R L+W
Sbjct: 1039 VSNIFKSLVGSMSCVEFVAEARETINRSLEW 1069
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 25.8 bits (54), Expect = 7.7
Identities = 17/70 (24%), Positives = 30/70 (42%), Gaps = 1/70 (1%)
Frame = +2
Query: 371 GGRQADDIISWLKKKTGPPAVEVTSAEQAKELIDANTVIVFGFFSD-QSSARAKTFLSTA 547
GGR D + +K+G V E +E+ + + F S+ +S K + T
Sbjct: 186 GGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDFRSEIESWTEHKPQIHTY 245
Query: 548 QVVDDQVFAI 577
Q+ D+Q +
Sbjct: 246 QLPDNQTITL 255
>SPCC1827.07c ||SPCP1E11.01c|SPX/EXS domain
protein|Schizosaccharomyces pombe|chr 3|||Manual
Length = 682
Score = 25.8 bits (54), Expect = 7.7
Identities = 18/57 (31%), Positives = 26/57 (45%)
Frame = +2
Query: 653 KRVKYEDEEITEDLLNAWVFVQSMPTIVEFSHETASKIFGGKIKYHLLIFLSKKMAI 823
K VK D+ + DL WV S T +FS T ++ I L +KK+A+
Sbjct: 200 KIVKKYDKTLHTDLQGFWVDYMSRYTFTDFSITTNWQLHVEDIYARLFTNHNKKLAL 256
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,311,408
Number of Sequences: 5004
Number of extensions: 64620
Number of successful extensions: 254
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 227
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 416455520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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