BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0803 (788 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g44200.1 68415.m05500 expressed protein 46 2e-05 At4g19190.1 68417.m02832 zinc knuckle (CCHC-type) family protein... 42 4e-04 At2g44195.1 68415.m05498 hypothetical protein 34 0.094 At2g21900.1 68415.m02602 WRKY family transcription factor contai... 30 2.0 At3g22320.1 68416.m02819 DNA-directed RNA polymerase, putative s... 29 3.5 At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PG... 28 6.1 At5g15280.1 68418.m01790 pentatricopeptide (PPR) repeat-containi... 28 8.1 At4g37730.1 68417.m05342 bZIP transcription factor family protei... 28 8.1 >At2g44200.1 68415.m05500 expressed protein Length = 493 Score = 46.4 bits (105), Expect = 2e-05 Identities = 22/47 (46%), Positives = 32/47 (68%) Frame = +2 Query: 56 YMCKKFFHPASRDNLKRVWMAEQKTDAYKKKQEELRLQYEKEQDLHE 196 ++ KK +H S N++ VW AEQK +A +KK EELRLQ +E++ E Sbjct: 5 FLNKKGWHTGSLRNIETVWKAEQKQEAEQKKLEELRLQILQERERSE 51 >At4g19190.1 68417.m02832 zinc knuckle (CCHC-type) family protein contains Pfam domain, PF00098: Zinc knuckle Length = 595 Score = 42.3 bits (95), Expect = 4e-04 Identities = 30/120 (25%), Positives = 53/120 (44%), Gaps = 5/120 (4%) Frame = +2 Query: 26 KNKMGKGFNN-YMCKKFFHPASRDNLKRVWMAEQKTDAYKKKQEELRLQYEKEQDLHEN- 199 K K G +++ ++ +K +HP S N +R W+AEQ + ++ EE+ ++ +EQ+ + Sbjct: 31 KTKSGTAWSHSFLNQKPWHPLSYPNQRRKWIAEQTHAQHDRRAEEVAREFAQEQEFFKQA 90 Query: 200 ---XXXXXXXXXXXXXXNFMYEPPPGVKKEREREDNEPEYKFEWQRKYNAPRESYCKGDN 370 +FMY PPG E + EYK E + + + DN Sbjct: 91 ALISKKEREKIETMKAVSFMYVRPPGYDPESAK---AAEYKDEKHKGQGSSTQDPVADDN 147 Score = 40.7 bits (91), Expect = 0.001 Identities = 33/123 (26%), Positives = 53/123 (43%), Gaps = 3/123 (2%) Frame = +2 Query: 317 EWQRKYNAPR-ESYCKGDNEIRDQPFGILVRNVRCIKCHKWGHINTDKECTMYTLSM--S 487 E++ NAPR E+ G R +PF + VRNV+C++C +GH + D++C + M Sbjct: 183 EFEVLKNAPRMETGIPG----RVKPFAVEVRNVKCLRCGNFGHQSGDRDCPLKDAVMPNE 238 Query: 488 EARALQASTSTPEEKEEKPDVPTLMQQMRDTGLIMKPGALPLTAAKVEDNDSTNLTEHDL 667 E R + T P P + L KPG P D+ + + D+ Sbjct: 239 ELRLKRDDPLTAIIAHTDPSEPLKWE------LKQKPGLSPPRGGFDPDDPNQQIVAEDI 292 Query: 668 ISQ 676 + Sbjct: 293 FDE 295 >At2g44195.1 68415.m05498 hypothetical protein Length = 107 Score = 34.3 bits (75), Expect = 0.094 Identities = 15/34 (44%), Positives = 24/34 (70%) Frame = +2 Query: 56 YMCKKFFHPASRDNLKRVWMAEQKTDAYKKKQEE 157 ++ KK +H S N+++VW AEQK +A ++K EE Sbjct: 5 FLNKKGWHTGSIRNVEKVWKAEQKHEAEQEKIEE 38 >At2g21900.1 68415.m02602 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 202 Score = 29.9 bits (64), Expect = 2.0 Identities = 14/41 (34%), Positives = 22/41 (53%), Gaps = 3/41 (7%) Frame = +2 Query: 257 PPPGVKKEREREDNEPEY---KFEWQRKYNAPRESYCKGDN 370 P VKK+ ER+ N P+Y +E + + +P YC D+ Sbjct: 137 PDCNVKKKIERDTNNPDYILTTYEGRHNHPSPSVVYCDSDD 177 >At3g22320.1 68416.m02819 DNA-directed RNA polymerase, putative similar to SP|P19388 DNA-directed RNA polymerase II 23 kDa polypeptide (EC 2.7.7.6) {Homo sapiens}; contains Pfam profiles PF03871: RNA polymerase Rpb5 N-terminal domain, PF01191: RNA polymerase Rpb5 C-terminal domain Length = 205 Score = 29.1 bits (62), Expect = 3.5 Identities = 14/49 (28%), Positives = 23/49 (46%) Frame = +2 Query: 554 TLMQQMRDTGLIMKPGALPLTAAKVEDNDSTNLTEHDLISQLSKKQRKS 700 TLMQ +RD G + L +T + N+ DL++ +K+ S Sbjct: 16 TLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKRNDNS 64 >At2g36910.1 68415.m04527 multidrug resistance P-glycoprotein (PGP1) identical to P-glycoprotein GI:3849833 from [Arabidopsis thaliana]; homologous to mammalian mdr gene,contains ATP-binding cassette; related to multi drug resistance proteins Length = 1286 Score = 28.3 bits (60), Expect = 6.1 Identities = 16/35 (45%), Positives = 23/35 (65%), Gaps = 1/35 (2%) Frame = -2 Query: 439 SPLMTLNASYIPDKYTKRLISDFIVS-FTIAFSRS 338 SP+MT N+SY Y++RL SDF S F+++ S Sbjct: 634 SPIMTRNSSYGRSPYSRRL-SDFSTSDFSLSIDAS 667 >At5g15280.1 68418.m01790 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 1227 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/35 (34%), Positives = 23/35 (65%), Gaps = 2/35 (5%) Frame = +2 Query: 320 WQRKYNAPRESYCKGDNEIR-DQPFGILVR-NVRC 418 + + YN + YCKG+N ++ ++ G++VR N+ C Sbjct: 837 YNKIYNVMFQGYCKGNNWMKVEEVLGLMVRKNIIC 871 >At4g37730.1 68417.m05342 bZIP transcription factor family protein contains Pfam profile: PF00170 bZIP transcription factor Length = 305 Score = 27.9 bits (59), Expect = 8.1 Identities = 14/48 (29%), Positives = 19/48 (39%) Frame = +2 Query: 14 QLVVKNKMGKGFNNYMCKKFFHPASRDNLKRVWMAEQKTDAYKKKQEE 157 QL G G NN+ KK P D KR M + A + + + Sbjct: 169 QLTAITNFGSGENNHNRKKMIQPEMTDERKRKRMESNRESAKRSRMRK 216 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,864,814 Number of Sequences: 28952 Number of extensions: 269771 Number of successful extensions: 930 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 897 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 929 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1775300800 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
- SilkBase 1999-2023 -