Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0797
(807 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 256 1e-68
At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protei... 66 3e-11
At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protei... 64 8e-11
At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protei... 54 8e-08
At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protei... 54 8e-08
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 33 0.22
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 33 0.29
At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilas... 31 0.68
At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P460... 31 1.2
At5g67240.1 68418.m08475 exonuclease family protein contains exo... 29 3.6
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 3.6
At5g06970.1 68418.m00789 expressed protein 29 4.8
At5g02250.1 68418.m00148 ribonuclease II family protein contains... 29 4.8
At5g45720.1 68418.m05621 hypothetical protein 28 6.3
At5g40740.1 68418.m04944 expressed protein 28 8.4
At2g39980.1 68415.m04913 transferase family protein contains Pfa... 28 8.4
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 256 bits (628), Expect = 1e-68
Identities = 126/224 (56%), Positives = 157/224 (70%), Gaps = 3/224 (1%)
Frame = +1
Query: 49 SLESIINNNLTGRDLEEFNRIHFGR---RNNLEIKLKESSIXXXXXXXXXXXXXXFPAKD 219
SL +++ NL +E NR+ GR R+ +I L ES+ F A
Sbjct: 22 SLHQLLSANLKPELYQEVNRLLLGRNCGRSLEQIVLPESAKALSSKHDFDLQAASFSADK 81
Query: 220 EQTRPPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNM 399
EQ R PR+V+VG++Q+SIA+PT P ++Q + IF+K+K IID AG GVNI+C QE W M
Sbjct: 82 EQMRNPRVVRVGLIQNSIALPTTAPFSDQTRGIFDKLKPIIDAAGVAGVNILCLQEAWTM 141
Query: 400 PFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVV 579
PFAFCTRE++ WCEFAE DG +T FL+ELA KY MVIVS ILERD H ++LWNTAV+
Sbjct: 142 PFAFCTRERR-WCEFAEPV-DGESTKFLQELAKKYNMVIVSPILERDIDHGEVLWNTAVI 199
Query: 580 ISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATRYGK 711
I + GN+IGKHRKNHIPRVGDFNES YYMEG+TGHPVF T +GK
Sbjct: 200 IGNNGNIIGKHRKNHIPRVGDFNESTYYMEGDTGHPVFETVFGK 243
Score = 64.1 bits (149), Expect = 1e-10
Identities = 26/32 (81%), Positives = 29/32 (90%)
Frame = +2
Query: 710 RIAVNICFGRHHVLNWMMFGQNGAEIVFNPSA 805
+IAVNIC+GRHH LNW+ FG NGAEIVFNPSA
Sbjct: 243 KIAVNICYGRHHPLNWLAFGLNGAEIVFNPSA 274
>At2g27450.1 68415.m03317 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 299
Score = 65.7 bits (153), Expect = 3e-11
Identities = 39/128 (30%), Positives = 71/128 (55%), Gaps = 1/128 (0%)
Frame = +1
Query: 331 KKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAM 510
++++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +
Sbjct: 31 ERLVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGV 89
Query: 511 VI-VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHP 687
VI VS E + H +N+ +I G +G +RK+HIP + E Y+ G+TG
Sbjct: 90 VIPVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFK 145
Query: 688 VFATRYGK 711
VF T++ K
Sbjct: 146 VFQTKFAK 153
>At2g27450.2 68415.m03318 carbon-nitrogen hydrolase family protein
low similarity to beta-alanine synthase [Drosophila
melanogaster] GI:14334063; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 326
Score = 64.5 bits (150), Expect = 8e-11
Identities = 39/126 (30%), Positives = 69/126 (54%), Gaps = 1/126 (0%)
Frame = +1
Query: 337 IIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAIKYAMVI 516
++ A +G NII QEL+ + FC +++ + + A+ ++ PT +++LA + +VI
Sbjct: 60 LVREAHAKGANIILIQELFE-GYYFCQAQREDFFKRAKPYKNHPTIARMQKLAKELGVVI 118
Query: 517 -VSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVF 693
VS E + H +N+ +I G +G +RK+HIP + E Y+ G+TG VF
Sbjct: 119 PVSFFEEANTAH----YNSIAIIDADGTDLGIYRKSHIPDGPGYQEKFYFNPGDTGFKVF 174
Query: 694 ATRYGK 711
T++ K
Sbjct: 175 QTKFAK 180
>At5g12040.2 68418.m01407 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 294
Score = 54.4 bits (125), Expect = 8e-08
Identities = 36/130 (27%), Positives = 61/130 (46%), Gaps = 2/130 (1%)
Frame = +1
Query: 295 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 468
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 469 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFN 648
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+ + D
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHLFDI-DIP 210
Query: 649 ESNYYMEGNT 678
+ME T
Sbjct: 211 GKITFMESKT 220
>At5g12040.1 68418.m01408 carbon-nitrogen hydrolase family protein
similar to Nit protein 2 [Homo sapiens] GI:9367116;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 369
Score = 54.4 bits (125), Expect = 8e-08
Identities = 36/130 (27%), Positives = 61/130 (46%), Gaps = 2/130 (1%)
Frame = +1
Query: 295 VNEQKKAIFNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQP-WCEFAESAED-GP 468
V KK + KK I+ A +G ++ E+WN P+ + + P + E ++ D P
Sbjct: 97 VTSDKKRNISHAKKAIEEAASKGAKLVLLPEIWNSPY---SNDSFPVYAEEIDAGGDASP 153
Query: 469 TTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFN 648
+T L E++ + + I+ + E+ D L+NT V G + KHRK H+ + D
Sbjct: 154 STAMLSEVSKRLKITIIGGSI--PERVGDRLYNTCCVFGSDGELKAKHRKIHLFDI-DIP 210
Query: 649 ESNYYMEGNT 678
+ME T
Sbjct: 211 GKITFMESKT 220
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 33.1 bits (72), Expect = 0.22
Identities = 43/142 (30%), Positives = 59/142 (41%), Gaps = 13/142 (9%)
Frame = +1
Query: 232 PPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQELW------ 393
P V+V IVQ S V D P A +K +K I A +G ++ F E +
Sbjct: 21 PSSTVRVTIVQSS-TVYNDTP------ATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPR 73
Query: 394 --NMPFAFCTREKQPWCEFAE---SA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHS 552
A ++ EF SA GP L ELA K + +V +E+D
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKD---G 130
Query: 553 DILWNTAVVISDTGNVIGKHRK 618
L+ TA+ S G +GKHRK
Sbjct: 131 YTLYCTALFFSPQGQFLGKHRK 152
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 32.7 bits (71), Expect = 0.29
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +1
Query: 463 GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 618
GP L ELA K + +V +E+D L+ TA+ S G +GKHRK
Sbjct: 97 GPEVEKLAELAGKNNVYLVMGAIEKD---GYTLYCTALFFSPQGQFLGKHRK 145
>At4g08790.1 68417.m01448 nitrilase, putative similar to nitrilase 1
[Mus musculus] GI:3228668; contains Pfam profile
PF00795: hydrolase, carbon-nitrogen family
Length = 307
Score = 31.5 bits (68), Expect = 0.68
Identities = 34/125 (27%), Positives = 54/125 (43%), Gaps = 7/125 (5%)
Frame = +1
Query: 319 FNKVKKIIDVAGQEGVNIICFQELWNMPFAFCTREKQPWCEFAESAEDGPTTTFLRELAI 498
F +++ A G +ICF E F+F ++ + AE DGP LA
Sbjct: 54 FATCSRLVQEAALAGAKLICFPE----NFSFVGDKEGESVKIAEPL-DGPVMERYCSLAR 108
Query: 499 KYAMVI-VSSILER-DEKHSDILWNTAVVISDTGNVIGKHRKNH-----IPRVGDFNESN 657
+ + + ER D+ H L NT VVI D G + ++K H +P + ES+
Sbjct: 109 DSNIWLSLGGFQERFDDTH---LCNTHVVIDDAGMIRDTYQKMHLFDVDVPGGSSYKESS 165
Query: 658 YYMEG 672
+ + G
Sbjct: 166 FTVPG 170
>At5g22300.1 68418.m02601 nitrilase 4 (NIT4) identical to SP|P46011
Nitrilase 4 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 355
Score = 30.7 bits (66), Expect = 1.2
Identities = 29/115 (25%), Positives = 51/115 (44%), Gaps = 13/115 (11%)
Frame = +1
Query: 313 AIFNKVKKIIDVAGQEGVNIICFQELW--------NMPFAFCTREKQPWCEFAE---SAE 459
A +K ++++ A + G ++ F E + A +R + +F + SA
Sbjct: 52 ATLDKAERLLSEAAENGSQLVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAI 111
Query: 460 D--GPTTTFLRELAIKYAMVIVSSILERDEKHSDILWNTAVVISDTGNVIGKHRK 618
D GP L +A KY + +V ++ER+ L+ T + G +GKHRK
Sbjct: 112 DVPGPEVERLALMAKKYKVYLVMGVIERE---GYTLYCTVLFFDSQGLFLGKHRK 163
>At5g67240.1 68418.m08475 exonuclease family protein contains
exonuclease domain, Pfam:PF00929
Length = 745
Score = 29.1 bits (62), Expect = 3.6
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 471 HDLPSGTRHQVRNGDRVLDIRKGREAFGH 557
HD+PS H V NGD LD++ + G+
Sbjct: 441 HDVPSQELHGVLNGDFTLDVKPPKRKGGY 469
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.1 bits (62), Expect = 3.6
Identities = 39/142 (27%), Positives = 58/142 (40%), Gaps = 13/142 (9%)
Frame = +1
Query: 232 PPRIVKVGIVQHSIAVPTDRPVNEQKKAIFNKVKKIIDVAGQEGVNIICFQE--LWNMPF 405
P V+V IVQ S V D P A +K +K I A +G ++ F E + P
Sbjct: 21 PSTTVRVTIVQSS-TVYNDTP------ATIDKAEKYIVEAASKGAELVLFPEGFIGGYPR 73
Query: 406 AFC---------TREKQPWCEFAESA--EDGPTTTFLRELAIKYAMVIVSSILERDEKHS 552
F + + ++ SA GP L ++A K + +V +E K
Sbjct: 74 GFRFGLAVGVHNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIE---KEG 130
Query: 553 DILWNTAVVISDTGNVIGKHRK 618
L+ T + S G +GKHRK
Sbjct: 131 YTLYCTVLFFSPQGQFLGKHRK 152
>At5g06970.1 68418.m00789 expressed protein
Length = 1101
Score = 28.7 bits (61), Expect = 4.8
Identities = 13/59 (22%), Positives = 27/59 (45%)
Frame = -2
Query: 614 RCFPITFPVSLITTAVFQSMSECFSSLSNIEDTITIAYLMASSRRKVVVGPSSADSANS 438
R I P + + ++ S LS +ED++ + ++ R K+V+ S + + S
Sbjct: 834 RSINIDVPATAMLCVQLNTLHYAVSQLSKLEDSMWLRWIAKKPREKIVIRKSMVEKSKS 892
>At5g02250.1 68418.m00148 ribonuclease II family protein contains
Pfam profile PF00773: RNB-like protein
Length = 803
Score = 28.7 bits (61), Expect = 4.8
Identities = 12/39 (30%), Positives = 18/39 (46%)
Frame = +2
Query: 683 ILYLRPDTARIAVNICFGRHHVLNWMMFGQNGAEIVFNP 799
+L + D R+ + + R NWM+F QNG P
Sbjct: 124 LLEFKKDADRVLLAVLHRRDGKKNWMVFDQNGVSCSIKP 162
>At5g45720.1 68418.m05621 hypothetical protein
Length = 900
Score = 28.3 bits (60), Expect = 6.3
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +1
Query: 577 VISDTGNVIGKHRKNHIPRVGDFNESNYYMEGNTGHPVFATR 702
V+ D+ IG+H +NHI + N+ N PV R
Sbjct: 9 VLKDSNGDIGEHLRNHIHLTNCIHLKNHMHNNNKQSPVLTDR 50
>At5g40740.1 68418.m04944 expressed protein
Length = 741
Score = 27.9 bits (59), Expect = 8.4
Identities = 14/42 (33%), Positives = 21/42 (50%)
Frame = +1
Query: 532 ERDEKHSDILWNTAVVISDTGNVIGKHRKNHIPRVGDFNESN 657
ER+ + ++ +++ NVIG N PRVG F SN
Sbjct: 8 ERELELESAMYTNCLLLGLDPNVIGLGASNGTPRVGLFRHSN 49
>At2g39980.1 68415.m04913 transferase family protein contains Pfam
profile PF02458 transferase family
Length = 482
Score = 27.9 bits (59), Expect = 8.4
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -3
Query: 589 CH*LQPQCSKVCPNASRPFLISRTRSPL 506
CH +Q C CPN P LIS + L
Sbjct: 39 CHYIQKGCLFTCPNLPLPALISHLKHSL 66
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,148,721
Number of Sequences: 28952
Number of extensions: 401092
Number of successful extensions: 1233
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 1184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1228
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1833827200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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