Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0774
         (805 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|c...   105   7e-24
SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3 |Sch...    32   0.083
SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosac...    31   0.14 
SPBC1198.08 |||dipeptidase Dug1 |Schizosaccharomyces pombe|chr 2...    29   0.58 
SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein 4|Schizosacc...    29   1.0  
SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual      27   3.1  
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch...    27   4.1  
SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha sub...    27   4.1  
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi...    26   5.5  
SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|ch...    26   7.2  
SPBC8E4.05c |||fumarate lyase superfamily|Schizosaccharomyces po...    26   7.2  
SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomy...    26   7.2  
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom...    25   9.5  

>SPAC1D4.09c |||DUF602 family protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 240

 Score =  105 bits (252), Expect = 7e-24
 Identities = 60/192 (31%), Positives = 101/192 (52%), Gaps = 3/192 (1%)
 Frame = +3

Query: 150 MGCDGGTIPRRDELVRMKKKPEQKDKDAERSFK---WRNCALSQQILQEPIVACSLGRLY 320
           MG DGG++P R+ELV+   K    D D +RS K   +  CA++ + L  PIV+C LG+LY
Sbjct: 1   MGNDGGSLPTRNELVKEPGKVPPLDIDFKRSVKSSQFSQCAITDEPLYPPIVSCGLGKLY 60

Query: 321 SKSSVLEALLDKETRPESINHXXXXXXXXXXXXXXXPAYVPTDHTDGTFDNGSAPYICPI 500
           +K+S+L+ LLD+ + P+S +H                  V  D      D+G   ++CPI
Sbjct: 61  NKASILQMLLDRSSVPKSPSH-------IKSLKDVVQLQVELD------DSGKVLWLCPI 107

Query: 501 SGLEMTGKFRFVFLWSCGCVLAERALKEVRQNLCHMCQQPFTDNDIVVLNGTEEDIEKLK 680
           +   M+  ++F ++  CG V    ALK+  + +C  C Q + + D++ +N   E ++ L 
Sbjct: 108 TRHVMSDTYQFAYIVPCGHVFEYSALKQFGEKMCFQCNQVYEEKDVIPINPNAEQLKTLS 167

Query: 681 DKMAIRVSNRKT 716
            ++     + KT
Sbjct: 168 KRLLDLALSEKT 179


>SPAC13G6.01c |rad8|SPAC5H10.14c|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1133

 Score = 32.3 bits (70), Expect = 0.083
 Identities = 14/34 (41%), Positives = 20/34 (58%), Gaps = 4/34 (11%)
 Frame = +3

Query: 549 CGCVLAERALKEVRQN----LCHMCQQPFTDNDI 638
           CG  L+E    + R+N    LCH C+QPF + D+
Sbjct: 898 CGDCLSEHIQYQKRRNIIPPLCHTCRQPFNEQDV 931


>SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 250

 Score = 31.5 bits (68), Expect = 0.14
 Identities = 20/75 (26%), Positives = 33/75 (44%), Gaps = 2/75 (2%)
 Frame = +3

Query: 129 KTHIFHKMGCDGGTIPRRDELVRMKKKPEQK-DKDAERSFKWRN-CALSQQILQEPIVAC 302
           K  I+H+   DG   P       +    EQ+ D+    S  + N C L+ Q +  PI++ 
Sbjct: 134 KKTIWHEQNTDGSDFPSMKTFFNVMNTEEQEADEVMVYSATFDNRCPLTLQPIVHPILST 193

Query: 303 SLGRLYSKSSVLEAL 347
           +    Y K ++L  L
Sbjct: 194 ACNHFYEKDAILSLL 208


>SPBC1198.08 |||dipeptidase Dug1 |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 474

 Score = 29.5 bits (63), Expect = 0.58
 Identities = 15/51 (29%), Positives = 25/51 (49%)
 Frame = +3

Query: 624 TDNDIVVLNGTEEDIEKLKDKMAIRVSNRKTLKRTRQRNWKSP*LKLKSIQ 776
           T  +  + +G +  +E LK+ +   +S     KRT Q  W+ P L L  I+
Sbjct: 269 TPTEDSIYDGIDYTMEDLKEAVGADISIYPDPKRTLQHRWRYPTLSLHGIE 319


>SPAC29A4.16 |hal4|sat4, ppk10|halotolerence protein
           4|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 636

 Score = 28.7 bits (61), Expect = 1.0
 Identities = 13/29 (44%), Positives = 16/29 (55%)
 Frame = +3

Query: 474 GSAPYICPISGLEMTGKFRFVFLWSCGCV 560
           GSAPYI P    E     R V +W+CG +
Sbjct: 522 GSAPYIAPEEYTESEFDPRAVDVWACGVI 550


>SPAPB15E9.02c |||dubious|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 188

 Score = 27.1 bits (57), Expect = 3.1
 Identities = 15/47 (31%), Positives = 24/47 (51%)
 Frame = -2

Query: 255 YAT*NFFQHLYLSVLASFSFERAHRVEELYRHHNPFCEKYVFFHYFF 115
           +AT + F  L+   L S  F R   ++  Y HH+ F  +  F+ +FF
Sbjct: 37  FATLHAFVFLFSHTLVSSQFRR---LKLPYHHHHTFTIEAFFYWFFF 80


>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 269

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 2/49 (4%)
 Frame = -1

Query: 634 SLSVNGC*HIWHRFCRTSFSALSASTQ--PQLQRKTKRNFPVISKPLIG 494
           +LS   C HI+  FC  S    +A+TQ  P  +RK   N  +  + ++G
Sbjct: 215 NLSCTPCGHIFCNFCILSALGTTAATQKCPVCRRKVHPNKVICLEMMLG 263


>SPCC1620.09c |tfg1||transcription factor TFIIF complex alpha
           subunit Tfg1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 490

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 10/21 (47%), Positives = 15/21 (71%)
 Frame = +3

Query: 627 DNDIVVLNGTEEDIEKLKDKM 689
           D +  ++ G EED +KLKDK+
Sbjct: 274 DEEAPIMEGNEEDNKKLKDKI 294


>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
           Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 690

 Score = 26.2 bits (55), Expect = 5.5
 Identities = 19/65 (29%), Positives = 30/65 (46%), Gaps = 9/65 (13%)
 Frame = +3

Query: 540 LWSCGCVLAE----RALKEVRQNLCHMCQQ-----PFTDNDIVVLNGTEEDIEKLKDKMA 692
           +WS GC+L E    +AL +  ++  H+C       PF  N I   + T +   K   K+ 
Sbjct: 562 VWSIGCILVELFTGQALFQTHEDSEHLCMMEKILGPFDRNMISRSSRTSQRFFKSDGKVR 621

Query: 693 IRVSN 707
             +SN
Sbjct: 622 YPLSN 626


>SPAC30.01c |sec72|sec7b|Sec7 domain|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 1822

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 13/37 (35%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
 Frame = -2

Query: 318 KVFQDCMPLLVLVVFAARAH-NYAT*NFFQHLYLSVL 211
           +VF+ C  +  L+VF+ RAH       FF+ +Y  +L
Sbjct: 478 QVFEVCCDIFYLMVFSLRAHFKQEIEVFFREVYFPML 514


>SPBC8E4.05c |||fumarate lyase superfamily|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 447

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 12/30 (40%), Positives = 16/30 (53%)
 Frame = -2

Query: 231 HLYLSVLASFSFERAHRVEELYRHHNPFCE 142
           +L  +V  +F F+ A  V  L RHH   CE
Sbjct: 155 NLQQAVPITFGFKMARFVATLRRHHQRLCE 184


>SPBP22H7.05c |||ATPase with bromodomain protein|Schizosaccharomyces
            pombe|chr 2|||Manual
          Length = 1201

 Score = 25.8 bits (54), Expect = 7.2
 Identities = 10/35 (28%), Positives = 23/35 (65%)
 Frame = +3

Query: 669  EKLKDKMAIRVSNRKTLKRTRQRNWKSP*LKLKSI 773
            +K+K+K+ +++S+   + R+R + +K P + L  I
Sbjct: 896  KKIKNKIQVKLSSILEMLRSRYKKFKKPIIDLNDI 930


>SPBC3F6.05 |rga1||GTPase activating protein
           Rga1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1150

 Score = 25.4 bits (53), Expect = 9.5
 Identities = 7/13 (53%), Positives = 12/13 (92%)
 Frame = -3

Query: 692 CHLVFQFFNVFLC 654
           CH++++F+NV LC
Sbjct: 267 CHMIYKFWNVKLC 279


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,362,841
Number of Sequences: 5004
Number of extensions: 70852
Number of successful extensions: 244
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 229
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 243
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 390427050
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)