Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0622
(690 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 81 7e-16
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 79 4e-15
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 77 1e-14
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 76 3e-14
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 73 1e-13
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 69 4e-12
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 61 6e-10
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 59 2e-09
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 56 2e-08
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 56 3e-08
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 50 1e-06
At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein... 30 1.3
At5g35860.1 68418.m04307 hypothetical protein contains Pfam prof... 30 1.7
At1g70500.1 68414.m08113 polygalacturonase, putative / pectinase... 28 6.7
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 81.0 bits (191), Expect = 7e-16
Identities = 30/67 (44%), Positives = 50/67 (74%)
Frame = +1
Query: 490 TLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHSRMA 669
++ E A H+ DDCW+VI +VYD+S+++DEHPGG D++L AG+DA+ F +GHS+ A
Sbjct: 9 SMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSKDA 68
Query: 670 VKALDRF 690
+ ++++
Sbjct: 69 RELMEKY 75
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 78.6 bits (185), Expect = 4e-15
Identities = 32/70 (45%), Positives = 46/70 (65%)
Frame = +1
Query: 481 RTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHS 660
+ TL+EV+ H + DCWIVI +VYD++ FLD+HPGG +++L G+DA+ F GHS
Sbjct: 6 KVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHS 65
Query: 661 RMAVKALDRF 690
A LD +
Sbjct: 66 STAKAMLDEY 75
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 77.0 bits (181), Expect = 1e-14
Identities = 31/68 (45%), Positives = 47/68 (69%)
Frame = +1
Query: 487 ITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHSRM 666
I+ +VA H +DCWI+I+ +VYDISTF+DEHPGG +++L G+DAS F HS+
Sbjct: 5 ISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKD 64
Query: 667 AVKALDRF 690
A + + ++
Sbjct: 65 AKELMKKY 72
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 75.8 bits (178), Expect = 3e-14
Identities = 29/70 (41%), Positives = 48/70 (68%)
Frame = +1
Query: 481 RTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHS 660
+ TL+EV+ H+ DCWIVI +VY+++ FL++HPGG D++L G+DA+ F GHS
Sbjct: 6 KIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHS 65
Query: 661 RMAVKALDRF 690
A + ++++
Sbjct: 66 ESAREMMEQY 75
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 73.3 bits (172), Expect = 1e-13
Identities = 26/70 (37%), Positives = 46/70 (65%)
Frame = +1
Query: 481 RTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHS 660
+ ++ EV+ H+ DCW++I +VYD++ F+D+HPGG +++L G+DA+ F GHS
Sbjct: 6 KVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHS 65
Query: 661 RMAVKALDRF 690
A +D++
Sbjct: 66 DTARDMMDKY 75
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 68.5 bits (160), Expect = 4e-12
Identities = 29/89 (32%), Positives = 56/89 (62%)
Frame = +1
Query: 424 NTEQKQWTEVVPGSPEAKDRTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDI 603
+ ++K+ + P + ++ + +EVA H+ +DCWI+I D+VYDI+++++EHP GGD
Sbjct: 28 DNQEKKRSSSEPVEDVVRPKSYSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHP-GGDA 86
Query: 604 MLEYAGRDASTAFRSSGHSRMAVKALDRF 690
+L++AG D++ F H+ ++ F
Sbjct: 87 ILDHAGDDSTDGFFGPQHATRVFDMIEDF 115
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 61.3 bits (142), Expect = 6e-10
Identities = 26/67 (38%), Positives = 42/67 (62%)
Frame = +1
Query: 490 TLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHSRMA 669
+++EV H++ D CWI+++ +YD + FL +HPGG D +L AG D + F + HS A
Sbjct: 546 SMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFEAI-HSDKA 604
Query: 670 VKALDRF 690
K L+ +
Sbjct: 605 KKMLEDY 611
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 59.3 bits (137), Expect = 2e-09
Identities = 26/67 (38%), Positives = 41/67 (61%)
Frame = +1
Query: 490 TLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAFRSSGHSRMA 669
+++EV H+T D WI+++ +YD + FL +HPGG D +L AG D + F + HS A
Sbjct: 549 SISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFEAI-HSDKA 607
Query: 670 VKALDRF 690
K L+ +
Sbjct: 608 KKLLEDY 614
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 56.0 bits (129), Expect = 2e-08
Identities = 24/58 (41%), Positives = 36/58 (62%)
Frame = +1
Query: 469 EAKDRTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAF 642
E+ R I + EV H T D W V+ RVY+IS +++ HPGG D++++ GRD + F
Sbjct: 123 ESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLF 180
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 55.6 bits (128), Expect = 3e-08
Identities = 23/58 (39%), Positives = 35/58 (60%)
Frame = +1
Query: 469 EAKDRTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAF 642
+ K R +T ++ H+ P D WI I +VYD+S ++ HPGG +L AG+D + AF
Sbjct: 4 QTKKRYVTSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 50.4 bits (115), Expect = 1e-06
Identities = 20/58 (34%), Positives = 34/58 (58%)
Frame = +1
Query: 469 EAKDRTITLAEVAHHDTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTAF 642
E + + IT ++ H+ D WI I +VY++S ++ HPGG ++L G+D + AF
Sbjct: 4 ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
>At2g24940.1 68415.m02982 cytochrome b5 domain-containing protein
similar to SP|P70580 Membrane associated progesterone
receptor component 1 {Rattus norvegicus}; contains Pfam
profile PF00173: Heme/Steroid binding domain
Length = 100
Score = 30.3 bits (65), Expect = 1.3
Identities = 15/42 (35%), Positives = 24/42 (57%)
Frame = +1
Query: 514 DTPDDCWIVIYDRVYDISTFLDEHPGGGDIMLEYAGRDASTA 639
D ++ I RV+D++T + GGD + +AG+DAS A
Sbjct: 15 DESKPIYVAIKGRVFDVTTGKSFYGSGGDYSM-FAGKDASRA 55
>At5g35860.1 68418.m04307 hypothetical protein contains Pfam profile
PF03778: Protein of unknown function (DUF321)
Length = 179
Score = 29.9 bits (64), Expect = 1.7
Identities = 11/26 (42%), Positives = 15/26 (57%)
Frame = +3
Query: 594 WRYNVGVRRPRRKHGFQEFWALSHGC 671
WR + +R R HGF FW ++GC
Sbjct: 6 WRKVIDLRFWRENHGFTFFWRENYGC 31
>At1g70500.1 68414.m08113 polygalacturonase, putative / pectinase,
putative similar to polygalacturonase [Cucumis sativus]
GI:6624205; contains Pfam profile PF00295: Glycosyl
hydrolases family 28 (polygalacturonases)
Length = 468
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/56 (28%), Positives = 26/56 (46%)
Frame = -2
Query: 524 SGVSWWATSAKVIVRSLASGEPGTTSVHCFCSVLTDQGLTKRKAAIVSAIRSKSAS 357
SG WWA S K + G P +++ +V +GLT R + + I +S +
Sbjct: 169 SGTKWWAASCKKNKSNPCVGAPTALTIYSSSNVYV-RGLTIRNSQQMHLIIQRSTT 223
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,058,831
Number of Sequences: 28952
Number of extensions: 303195
Number of successful extensions: 823
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 822
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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