Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0596
(693 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b... 96 2e-20
At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to SP... 91 8e-19
At1g26340.1 68414.m03212 cytochrome b5, putative similar to cyto... 89 3e-18
At2g32720.1 68415.m04004 cytochrome b5, putative similar to Cyto... 88 5e-18
At2g46650.1 68415.m05820 cytochrome b5, putative similar to cyto... 88 6e-18
At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to ... 74 8e-14
At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to ... 74 8e-14
At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein... 57 1e-08
At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1) ... 51 8e-07
At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putati... 48 4e-06
At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein... 40 0.002
At1g77670.1 68414.m09043 aminotransferase class I and II family ... 31 0.96
At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibit... 29 2.9
At2g41700.1 68415.m05151 ABC transporter family protein similar ... 29 3.9
At1g17810.2 68414.m02205 major intrinsic family protein / MIP fa... 29 3.9
At1g17810.1 68414.m02204 major intrinsic family protein / MIP fa... 29 3.9
At3g63090.1 68416.m07086 expressed protein 28 5.1
At5g36790.1 68418.m04408 phosphoglycolate phosphatase, putative ... 28 6.8
At5g36700.1 68418.m04392 phosphoglycolate phosphatase, putative ... 28 6.8
>At5g48810.1 68418.m06039 cytochrome b5 identical to cytochrome b5
[Arabidopsis thaliana] GI:4240122; strong similarity to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 140
Score = 95.9 bits (228), Expect = 2e-20
Identities = 47/128 (36%), Positives = 77/128 (60%)
Frame = +3
Query: 156 MEGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFE 335
M G+ K+FT E+ S+ D ++I +VYDVTKFL +HPGG+EV+L G+DA++ FE
Sbjct: 1 MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60
Query: 336 DVSHSSDARSLMKKYKIGELVESDRVQTRAGFSPQWTNEQAQEQGNWSSWLTPLLLGVAA 515
DV HSS A++++ +Y +G+ +++ V +A F P + + Q S ++ LL +
Sbjct: 61 DVGHSSTAKAMLDEYYVGD-IDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVP 119
Query: 516 TLLYRYLF 539
L+ F
Sbjct: 120 LLILGLAF 127
>At5g53560.1 68418.m06655 cytochrome b5 isoform 1 identical to
SP|Q42342 Cytochrome b5 isoform 1 [Arabidopsis thaliana]
Length = 134
Score = 90.6 bits (215), Expect = 8e-19
Identities = 44/95 (46%), Positives = 58/95 (61%)
Frame = +3
Query: 156 MEGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFE 335
M + K+ + EE+ + D LII +VYDVT F+ +HPGG+EVLL G+DA+ FE
Sbjct: 1 MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60
Query: 336 DVSHSSDARSLMKKYKIGELVESDRVQTRAGFSPQ 440
DV HS AR +M KY IGE+ S TR +PQ
Sbjct: 61 DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQ 95
>At1g26340.1 68414.m03212 cytochrome b5, putative similar to
cytochrome b5 GB:BAA74839 GI:4240120 from [Arabidopsis
thaliana]
Length = 135
Score = 88.6 bits (210), Expect = 3e-18
Identities = 40/78 (51%), Positives = 57/78 (73%)
Frame = +3
Query: 171 KLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFEDVSHS 350
KL++ EE + + + D ++I +VYDV+ ++ EHPGG++VLL AG+DA++ FED HS
Sbjct: 6 KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65
Query: 351 SDARSLMKKYKIGELVES 404
DAR LM+KY IGEL ES
Sbjct: 66 KDARELMEKYFIGELDES 83
>At2g32720.1 68415.m04004 cytochrome b5, putative similar to
Cytochrome B5 SP:P49098 from [Nicotiana tabacum]
Length = 134
Score = 88.2 bits (209), Expect = 5e-18
Identities = 38/94 (40%), Positives = 62/94 (65%)
Frame = +3
Query: 156 MEGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFE 335
M E K+FT E+ + D ++I+ +VY+VTKFL +HPGG++VLL G+DA++ FE
Sbjct: 1 MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60
Query: 336 DVSHSSDARSLMKKYKIGELVESDRVQTRAGFSP 437
DV HS AR +M++Y +GE ++ + + ++P
Sbjct: 61 DVGHSESAREMMEQYYVGE-IDPTTIPKKVKYTP 93
>At2g46650.1 68415.m05820 cytochrome b5, putative similar to
cytochome b5 GI:2695711 from [Olea europaea]
Length = 132
Score = 87.8 bits (208), Expect = 6e-18
Identities = 51/126 (40%), Positives = 71/126 (56%), Gaps = 8/126 (6%)
Frame = +3
Query: 174 LFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFEDVSHSS 353
L + ++ + D ++IH +VYD++ F+ EHPGG+ VLL G+DAS FEDV+HS
Sbjct: 4 LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63
Query: 354 DARSLMKKYKIGELVESDRVQTRAGFSPQWTNEQA------QEQG-NWSSWLTPLL-LGV 509
DA+ LMKKY IG+ V+ V + P W E +E G +L PLL LGV
Sbjct: 64 DAKELMKKYCIGD-VDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGV 122
Query: 510 AATLLY 527
A L +
Sbjct: 123 AFALRF 128
>At1g77760.1 68414.m09053 nitrate reductase 1 (NR1) identical to
SP|P11832 Nitrate reductase 1 (formerly EC 1.6.6.1)
(NR1){Arabidopsis thaliana}
Length = 917
Score = 74.1 bits (174), Expect = 8e-14
Identities = 32/81 (39%), Positives = 52/81 (64%)
Frame = +3
Query: 156 MEGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFE 335
M K+++ E++ +T A +I+H +YD T+FL +HPGG + +L AG D +E FE
Sbjct: 541 MNTASKMYSISEVRKHNTADSAWIIVHGHIYDCTRFLKDHPGGTDSILINAGTDCTEEFE 600
Query: 336 DVSHSSDARSLMKKYKIGELV 398
+ HS A+ L++ Y+IGEL+
Sbjct: 601 AI-HSDKAKKLLEDYRIGELI 620
>At1g37130.1 68414.m04639 nitrate reductase 2 (NR2) identical to
SP|P11035 Nitrate reductase 2 (formerly EC 1.6.6.1)
(NR2) {Arabidopsis thaliana}
Length = 917
Score = 74.1 bits (174), Expect = 8e-14
Identities = 30/81 (37%), Positives = 52/81 (64%)
Frame = +3
Query: 156 MEGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFE 335
M K+++ E+K ++ +I+H +YD T+FL++HPGG + +L AG D +E FE
Sbjct: 538 MNTTAKMYSMSEVKKHNSADSCWIIVHGHIYDCTRFLMDHPGGSDSILINAGTDCTEEFE 597
Query: 336 DVSHSSDARSLMKKYKIGELV 398
+ HS A+ +++ Y+IGEL+
Sbjct: 598 AI-HSDKAKKMLEDYRIGELI 617
>At1g60660.1 68414.m06829 cytochrome b5 domain-containing protein
contains InterPro accession IPR001199: Cytochrome b5
Length = 121
Score = 56.8 bits (131), Expect = 1e-08
Identities = 25/75 (33%), Positives = 48/75 (64%)
Frame = +3
Query: 171 KLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFEDVSHS 350
K +++ E+ + R D +II ++VYD+T ++ EHPGG + +L+ AG D+++ F H+
Sbjct: 47 KSYSKSEVAVHNKRNDCWIIIKDKVYDITSYVEEHPGG-DAILDHAGDDSTDGFFGPQHA 105
Query: 351 SDARSLMKKYKIGEL 395
+ +++ + IGEL
Sbjct: 106 TRVFDMIEDFYIGEL 120
>At3g61580.1 68416.m06897 delta-8 sphingolipid desaturase (SLD1)
identical to delta-8 sphingolipid desaturase GI:3819710
from [Arabidopsis thaliana]; contains Pfam profile
PF00487: Fatty acid desaturase; contains Pfam profile
PF00173: Heme/Steroid binding domain
Length = 449
Score = 50.8 bits (116), Expect = 8e-07
Identities = 22/58 (37%), Positives = 34/58 (58%)
Frame = +3
Query: 159 EGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPF 332
E E K T E+LK + D + I +VY+V+ ++ HPGG+ V+L GQD ++ F
Sbjct: 4 ETEKKYITNEDLKKHNKSGDLWIAIQGKVYNVSDWIKTHPGGDTVILNLVGQDVTDAF 61
Score = 29.9 bits (64), Expect = 1.7
Identities = 14/59 (23%), Positives = 32/59 (54%)
Frame = +3
Query: 54 FDRFFTYLTIQKYI*SYKQIGGSVVI*FKLINIIMEGEMKLFTREELKSRSTRADAVLI 230
+DR T+ + +++ SY+ V+ F IN+ ++ + LF++ E+ R+ +L+
Sbjct: 234 YDRKLTFDPVARFLVSYQHFTYYPVMCFGRINLFIQTFLLLFSKREVPDRALNFAGILV 292
>At2g46210.1 68415.m05746 delta-8 sphingolipid desaturase, putative
similar to delta-8 sphingolipid desaturase GI:3819708
from [Brassica napus]
Length = 449
Score = 48.4 bits (110), Expect = 4e-06
Identities = 21/51 (41%), Positives = 31/51 (60%)
Frame = +3
Query: 180 TREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPF 332
T E+LK + D + I +VYDV+ ++ HPGGE +L AGQD ++ F
Sbjct: 11 TSEDLKKHNKPGDLWISIQGKVYDVSDWVKSHPGGEAAILNLAGQDVTDAF 61
>At5g09680.1 68418.m01120 cytochrome b5 domain-containing protein
flavohemoprotein b5+b5R, Homo sapiens, EMBL:AF169803
Length = 211
Score = 39.5 bits (88), Expect = 0.002
Identities = 19/79 (24%), Positives = 39/79 (49%)
Frame = +3
Query: 159 EGEMKLFTREELKSRSTRADAVLIIHNEVYDVTKFLLEHPGGEEVLLEKAGQDASEPFED 338
E +L +E+K T ++ VY+++ ++ HPGG ++L++ G+D + F
Sbjct: 123 ESNKRLIPMDEVKKHRTGDSMWTVLKGRVYNISPYMNFHPGGVDMLMKAVGRDGTLLFNK 182
Query: 339 VSHSSDARSLMKKYKIGEL 395
+ L++K +G L
Sbjct: 183 YHAWVNVDILLEKCLVGVL 201
>At1g77670.1 68414.m09043 aminotransferase class I and II family
protein similar to kynurenine aminotransferase
/glutamine transaminase K GI:1030066 [Rattus norvegicus]
Length = 440
Score = 30.7 bits (66), Expect = 0.96
Identities = 15/37 (40%), Positives = 25/37 (67%), Gaps = 2/37 (5%)
Frame = +3
Query: 171 KLFTREELKSRSTRA--DAVLIIHNEVYDVTKFLLEH 275
K+FTREEL++ ++ + VL+ +EVYD F ++H
Sbjct: 227 KMFTREELETIASLCIENDVLVFSDEVYDKLAFEMDH 263
>At1g62760.1 68414.m07083 invertase/pectin methylesterase inhibitor
family protein low similarity to extensin [Volvox
carteri] GI:21992
Length = 312
Score = 29.1 bits (62), Expect = 2.9
Identities = 29/89 (32%), Positives = 39/89 (43%), Gaps = 4/89 (4%)
Frame = -3
Query: 523 SSVAATPSSSGVSQELQLPCSCACSLVHCGLKPARVCTRSLSTNS----PILYFFINDLA 356
SS + +PSSS S P S S L P+ SLS +S P ++ L+
Sbjct: 23 SSSSLSPSSSSPSLSPSPPSSSPSSAPPSSLSPSSPPPLSLSPSSPPPPPPSSSPLSSLS 82
Query: 355 SELWLTSSNGSEAS*PAFSRSTSSPPGCS 269
L + + S +S P S S SSPP S
Sbjct: 83 PSLSPSPPSSSPSSAPPSSLSPSSPPPLS 111
>At2g41700.1 68415.m05151 ABC transporter family protein similar to
ATP-binding cassette transporter ABCA1 GI:18031705 from
[Arabidopsis thaliana]
Length = 1822
Score = 28.7 bits (61), Expect = 3.9
Identities = 13/37 (35%), Positives = 24/37 (64%)
Frame = +3
Query: 51 QFDRFFTYLTIQKYI*SYKQIGGSVVI*FKLINIIME 161
QFD F YLT+++++ Y +I G V+ ++ N++ E
Sbjct: 1478 QFDALFEYLTVKEHLELYARIKG--VVDHRIDNVVTE 1512
>At1g17810.2 68414.m02205 major intrinsic family protein / MIP
family protein contains Pfam profile: MIP PF00230
Length = 225
Score = 28.7 bits (61), Expect = 3.9
Identities = 9/30 (30%), Positives = 16/30 (53%)
Frame = +3
Query: 471 NWSSWLTPLLLGVAATLLYRYLFV*ATRSP 560
+W W+ P + G A L+Y Y+ + + P
Sbjct: 182 HWIYWVGPFIGGALAALIYEYMIIPSVNEP 211
>At1g17810.1 68414.m02204 major intrinsic family protein / MIP
family protein contains Pfam profile: MIP PF00230
Length = 267
Score = 28.7 bits (61), Expect = 3.9
Identities = 9/30 (30%), Positives = 16/30 (53%)
Frame = +3
Query: 471 NWSSWLTPLLLGVAATLLYRYLFV*ATRSP 560
+W W+ P + G A L+Y Y+ + + P
Sbjct: 224 HWIYWVGPFIGGALAALIYEYMIIPSVNEP 253
>At3g63090.1 68416.m07086 expressed protein
Length = 404
Score = 28.3 bits (60), Expect = 5.1
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = +3
Query: 312 QDASEPFEDVSHSSDARSLMKKYKIGELVE 401
QD P+EDVSH A M+K +G + E
Sbjct: 258 QDYISPYEDVSHLDQASKEMEKRTVGVVHE 287
>At5g36790.1 68418.m04408 phosphoglycolate phosphatase, putative
similar to phosphoglycolate phosphatase precursor
[Chlamydomonas reinhardtii] GI:15982558; contains
InterPro accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 362
Score = 27.9 bits (59), Expect = 6.8
Identities = 10/39 (25%), Positives = 24/39 (61%)
Frame = -3
Query: 277 GCSRRNFVTS*TSLWIISTASARVDRDFSSSRVNNFISP 161
GC ++ TS+ ++ + ++ DF +S++++F+SP
Sbjct: 319 GCKTLLVLSGVTSISMLESPENKIQPDFYTSKISDFLSP 357
>At5g36700.1 68418.m04392 phosphoglycolate phosphatase, putative
similar to phosphoglycolate phosphatase precursor
[Chlamydomonas reinhardtii] GI:15982558; contains
InterPro accession IPR005834: Haloacid dehalogenase-like
hydrolase
Length = 362
Score = 27.9 bits (59), Expect = 6.8
Identities = 10/39 (25%), Positives = 24/39 (61%)
Frame = -3
Query: 277 GCSRRNFVTS*TSLWIISTASARVDRDFSSSRVNNFISP 161
GC ++ TS+ ++ + ++ DF +S++++F+SP
Sbjct: 319 GCKTLLVLSGVTSISMLESPENKIQPDFYTSKISDFLSP 357
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,737,431
Number of Sequences: 28952
Number of extensions: 225811
Number of successful extensions: 651
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 626
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 648
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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