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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0594
         (691 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g18540.1 68415.m02160 cupin family protein contains Pfam prof...    34   0.077
At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)...    32   0.41 
At2g25980.1 68415.m03120 jacalin lectin family protein similar t...    31   0.54 
At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH) domain...    30   1.3  
At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH) domain...    30   1.3  
At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH) domain...    30   1.3  
At3g58480.1 68416.m06518 calmodulin-binding family protein conta...    29   2.9  
At2g28910.1 68415.m03513 CAX-interacting protein 4 (CAXIP4) cont...    29   2.9  
At5g54480.1 68418.m06784 hypothetical protein                          29   3.8  
At4g06479.1 68417.m00885 hypothetical protein                          28   5.1  
At4g38040.1 68417.m05373 exostosin family protein contains Pfam ...    28   6.7  
At4g16680.1 68417.m02519 RNA helicase, putative similar to SP|Q1...    28   6.7  
At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein ...    28   6.7  
At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar...    27   8.9  

>At2g18540.1 68415.m02160 cupin family protein contains Pfam profile
           PF00190: Cupin
          Length = 707

 Score = 34.3 bits (75), Expect = 0.077
 Identities = 16/47 (34%), Positives = 30/47 (63%)
 Frame = +3

Query: 525 KRKDFFGQRKQREFIPDSKKDDGYWDRRRRNNEAAKRSREKRRFNDM 665
           K+++   QRK+RE +   ++++   +R+RR  EA KR  E++R  +M
Sbjct: 531 KKREEERQRKEREEVERKRREE--QERKRREEEARKREEERKREEEM 575



 Score = 32.3 bits (70), Expect = 0.31
 Identities = 18/48 (37%), Positives = 29/48 (60%), Gaps = 3/48 (6%)
 Frame = +3

Query: 546 QRKQREFIPDSKKDDGYWDR---RRRNNEAAKRSREKRRFNDMVLEQR 680
           QRK+RE +   ++++    R   R+R  EAAKR+ E+RR  +   E+R
Sbjct: 648 QRKEREDVERKRREEEAMRREEERKREEEAAKRAEEERRKKEEEEEKR 695


>At1g09080.1 68414.m01013 luminal binding protein 3 (BiP-3) (BP3)
           Similar to Arabidopsis luminal binding protein
           (gb|D89342); contains Pfam domain PF00012: dnaK protein
          Length = 678

 Score = 31.9 bits (69), Expect = 0.41
 Identities = 19/52 (36%), Positives = 29/52 (55%), Gaps = 1/52 (1%)
 Frame = +3

Query: 486 SSGDSHDYGA-FDFKRKDFFGQRKQREFIPDSKKDDGYWDRRRRNNEAAKRS 638
           +SGD+H  G  FD +  D+F +  ++++  D  KD     + RR  E AKRS
Sbjct: 267 TSGDTHLGGEDFDHRVMDYFIKLVKKKYNKDISKDHKALGKLRRECELAKRS 318


>At2g25980.1 68415.m03120 jacalin lectin family protein similar to
           myrosinase-binding protein homolog [Arabidopsis
           thaliana] GI:2997767; contains Pfam profile PF01419
           jacalin-like lectin domain
          Length = 449

 Score = 31.5 bits (68), Expect = 0.54
 Identities = 15/36 (41%), Positives = 20/36 (55%)
 Frame = +3

Query: 441 VSPHFPMDQAPPSNGSSGDSHDYGAFDFKRKDFFGQ 548
           + P  P ++   S G  G+S D GAFD  RK + GQ
Sbjct: 297 IPPPPPTEKLQGSGGDGGESWDDGAFDGVRKIYVGQ 332


>At2g43160.3 68415.m05361 epsin N-terminal homology (ENTH)
           domain-containing protein contains Pfam PF01417: ENTH
           domain. ENTH (Epsin N-terminal homology) domain; similar
           to Af10-protein (GI:1724114) [Avena fatua]
          Length = 895

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 21/70 (30%), Positives = 34/70 (48%)
 Frame = +3

Query: 480 NGSSGDSHDYGAFDFKRKDFFGQRKQREFIPDSKKDDGYWDRRRRNNEAAKRSREKRRFN 659
           +G  GD +DYG+ D +R  +  +R+      D    DG  DR  R++E  +  R+  R +
Sbjct: 172 SGGYGDKYDYGSRDEERSSYGREREYGYRDDDRNSRDG--DRHSRDSE-DRYGRDGNRDD 228

Query: 660 DMVLEQRVVE 689
           D     R V+
Sbjct: 229 DYRGRSRSVD 238


>At2g43160.2 68415.m05360 epsin N-terminal homology (ENTH)
           domain-containing protein contains Pfam PF01417: ENTH
           domain. ENTH (Epsin N-terminal homology) domain; similar
           to Af10-protein (GI:1724114) [Avena fatua]
          Length = 895

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 21/70 (30%), Positives = 34/70 (48%)
 Frame = +3

Query: 480 NGSSGDSHDYGAFDFKRKDFFGQRKQREFIPDSKKDDGYWDRRRRNNEAAKRSREKRRFN 659
           +G  GD +DYG+ D +R  +  +R+      D    DG  DR  R++E  +  R+  R +
Sbjct: 172 SGGYGDKYDYGSRDEERSSYGREREYGYRDDDRNSRDG--DRHSRDSE-DRYGRDGNRDD 228

Query: 660 DMVLEQRVVE 689
           D     R V+
Sbjct: 229 DYRGRSRSVD 238


>At2g43160.1 68415.m05359 epsin N-terminal homology (ENTH)
           domain-containing protein contains Pfam PF01417: ENTH
           domain. ENTH (Epsin N-terminal homology) domain; similar
           to Af10-protein (GI:1724114) [Avena fatua]
          Length = 895

 Score = 30.3 bits (65), Expect = 1.3
 Identities = 21/70 (30%), Positives = 34/70 (48%)
 Frame = +3

Query: 480 NGSSGDSHDYGAFDFKRKDFFGQRKQREFIPDSKKDDGYWDRRRRNNEAAKRSREKRRFN 659
           +G  GD +DYG+ D +R  +  +R+      D    DG  DR  R++E  +  R+  R +
Sbjct: 172 SGGYGDKYDYGSRDEERSSYGREREYGYRDDDRNSRDG--DRHSRDSE-DRYGRDGNRDD 228

Query: 660 DMVLEQRVVE 689
           D     R V+
Sbjct: 229 DYRGRSRSVD 238


>At3g58480.1 68416.m06518 calmodulin-binding family protein contains
           Pfam profile PF00612: IQ calmodulin-binding motif
          Length = 575

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 10/22 (45%), Positives = 15/22 (68%)
 Frame = +1

Query: 79  HCDSEFTFIFFLDVKSGSEI*H 144
           HCDS+  F ++LD+  G E+ H
Sbjct: 206 HCDSKQPFFYWLDIGQGKELNH 227


>At2g28910.1 68415.m03513 CAX-interacting protein 4 (CAXIP4)
           contains Pfam domain PF00098: Zinc knuckle; identical to
           cDNA CAX-interacting protein 4 GI:27651998
          Length = 332

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 13/36 (36%), Positives = 19/36 (52%)
 Frame = +3

Query: 546 QRKQREFIPDSKKDDGYWDRRRRNNEAAKRSREKRR 653
           ++K++    +S  D    DR+RR     KRS  KRR
Sbjct: 179 RKKKKRVSDESDSDSDSGDRKRRRRSMKKRSSHKRR 214


>At5g54480.1 68418.m06784 hypothetical protein 
          Length = 720

 Score = 28.7 bits (61), Expect = 3.8
 Identities = 13/48 (27%), Positives = 25/48 (52%)
 Frame = +3

Query: 519 DFKRKDFFGQRKQREFIPDSKKDDGYWDRRRRNNEAAKRSREKRRFND 662
           D    D F + ++RE IPD + +  Y    R+N +  K+ ++K+   +
Sbjct: 191 DEVESDGFREIREREGIPDLEPESDYNSLIRKNKKKNKKKKKKKNVRE 238


>At4g06479.1 68417.m00885 hypothetical protein
          Length = 370

 Score = 28.3 bits (60), Expect = 5.1
 Identities = 12/39 (30%), Positives = 19/39 (48%)
 Frame = +3

Query: 567 IPDSKKDDGYWDRRRRNNEAAKRSREKRRFNDMVLEQRV 683
           IP+S K D  W+      +      E  R++ M+LEQ +
Sbjct: 168 IPNSSKADSCWEDEMFKGDEPSEDEENGRWDKMLLEQEL 206


>At4g38040.1 68417.m05373 exostosin family protein contains Pfam
           profile: PF03016 Exostosin family
          Length = 425

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 15/33 (45%), Positives = 18/33 (54%)
 Frame = +2

Query: 50  HSHWIFRHNNIVTVNLHLYFFWT*KVGVKFDIF 148
           HS ++  HNN+V V  H  F W     VKFD F
Sbjct: 380 HSEFVSLHNNLVKVQKH--FQWN-SPPVKFDAF 409


>At4g16680.1 68417.m02519 RNA helicase, putative similar to
           SP|Q14562 ATP-dependent helicase DDX8 (RNA helicase
           HRH1) (DEAH-box protein 8) {Homo sapiens}; contains Pfam
           profiles PF04408: Helicase associated domain (HA2),
           PF00271: Helicase conserved C-terminal domain
          Length = 883

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 14/40 (35%), Positives = 23/40 (57%)
 Frame = +3

Query: 570 PDSKKDDGYWDRRRRNNEAAKRSREKRRFNDMVLEQRVVE 689
           PD+   +G  D+++R + A +R  E+RR   +V EQ   E
Sbjct: 119 PDAYDQEGCIDQKKRFDVAKERYCERRRSGRVVTEQEAWE 158


>At4g06634.1 68417.m01050 zinc finger (C2H2 type) family protein
           contains Pfam PF00096: Zinc finger, C2H2 type
          Length = 387

 Score = 27.9 bits (59), Expect = 6.7
 Identities = 9/12 (75%), Positives = 10/12 (83%)
 Frame = -2

Query: 606 CGPSSRRPFCCP 571
           CGPSS RP+ CP
Sbjct: 222 CGPSSDRPYACP 233


>At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to
           RNA helicase [Rattus norvegicus] GI:897915; contains
           Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271:
           Helicase conserved C-terminal domain
          Length = 1166

 Score = 27.5 bits (58), Expect = 8.9
 Identities = 15/57 (26%), Positives = 34/57 (59%), Gaps = 4/57 (7%)
 Frame = +3

Query: 519 DFKRKDFFGQRKQREFIPDSKK---DDGYWDRRRRNNE-AAKRSREKRRFNDMVLEQ 677
           D +R++   + +++E + + ++   +DG  DRR R  E  ++R+RE+ R  ++  E+
Sbjct: 153 DRERREREREEREKERVKERERREREDGERDRREREKERGSRRNRERERSREVGNEE 209


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,927,146
Number of Sequences: 28952
Number of extensions: 229033
Number of successful extensions: 700
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 665
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 697
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1467502800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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