Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0589
(475 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g23820.1 68416.m02994 NAD-dependent epimerase/dehydratase fam... 31 0.30
At1g22160.1 68414.m02770 senescence-associated protein-related s... 31 0.30
At5g25410.1 68418.m03014 expressed protein contains Pfam profil... 31 0.53
At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P460... 30 0.70
At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P329... 29 1.2
At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P329... 29 1.2
At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P329... 29 1.2
At3g60140.1 68416.m06715 glycosyl hydrolase family 1 protein con... 29 1.6
At3g10500.1 68416.m01260 no apical meristem (NAM) family protein... 29 1.6
At2g24370.1 68415.m02912 protein kinase family protein contains ... 28 3.7
At5g35730.1 68418.m04274 EXS family protein / ERD1/XPR1/SYG1 fam... 27 4.9
At5g25040.1 68418.m02967 integral membrane transporter family pr... 27 4.9
At4g16270.1 68417.m02468 peroxidase 40 (PER40) (P40) identical t... 27 6.5
At1g77860.1 68414.m09074 rhomboid family protein contains PFAM d... 27 6.5
At1g50410.1 68414.m05650 SNF2 domain-containing protein / helica... 27 8.6
>At3g23820.1 68416.m02994 NAD-dependent epimerase/dehydratase family
protein similar to nucleotide sugar epimerase from
Vibrio vulnificus GI:3093975 [PID:g3093975], WbnF
[Escherichia coli] GI:5739472, CAPI protein
{Staphylococcus aureus} SP|P39858; contains Pfam
profile: PF01370 NAD dependent epimerase/dehydratase
family
Length = 460
Score = 31.5 bits (68), Expect = 0.30
Identities = 15/30 (50%), Positives = 17/30 (56%)
Frame = +1
Query: 271 LIKKPKNGHVPSTHRTHHSAYKGGMYRTIT 360
LIK P+NG VP TH AYK Y+ T
Sbjct: 397 LIKMPRNGDVPYTHANVSLAYKDFGYKPTT 426
>At1g22160.1 68414.m02770 senescence-associated protein-related
similar to senescence-associated protein SAG102
(GI:22331931) [Arabidopsis thaliana]
Length = 147
Score = 31.5 bits (68), Expect = 0.30
Identities = 19/65 (29%), Positives = 35/65 (53%), Gaps = 2/65 (3%)
Frame = +3
Query: 102 VMPQGTVP--TASFSEEYLKPEQLYASTMLHGSNSEFFQELDQLCSDPQREEQTRTIDSH 275
V P+GT ++ +SE++L+ L ++HG + ++ CS R++Q T+D
Sbjct: 61 VSPRGTQRRHSSDYSEDFLRSCSLCKRLLVHGRDIYMYRGDRAFCSLECRQQQI-TVDER 119
Query: 276 KETEK 290
KE +K
Sbjct: 120 KEKKK 124
>At5g25410.1 68418.m03014 expressed protein contains Pfam profile
PF03080: Arabidopsis proteins of unknown function
Length = 369
Score = 30.7 bits (66), Expect = 0.53
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +3
Query: 108 PQGTVPTASFSEEYLKPEQLYASTMLHGSNSE 203
P GTVP S+EY+ QL+A H +SE
Sbjct: 92 PDGTVPILRNSKEYITNAQLFAEKYFHPLSSE 123
>At3g44320.1 68416.m04760 nitrilase 3 (NIT3) identical to SP|P46010
Nitrilase 3 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 30.3 bits (65), Expect = 0.70
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -2
Query: 426 HRKPERSCPCRCYYKSVPGRRDSYRPVHPTLVGRMMCAVCRRN-MPVFR-FLYENQWSVF 253
HRK + RC + G S PV+ T +G++ A+C N MP++R LY ++
Sbjct: 150 HRKVMPTSLERCIWGQGDG---STIPVYDTPIGKIGAAICWENRMPLYRTALYAKGIEIY 206
Query: 252 VLPFVDRS 229
P D S
Sbjct: 207 CAPTADYS 214
>At3g44310.2 68416.m04759 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 224
Score = 29.5 bits (63), Expect = 1.2
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -2
Query: 426 HRKPERSCPCRCYYKSVPGRRDSYRPVHPTLVGRMMCAVCRRN-MPVFR-FLYENQWSVF 253
HRK + RC + G S PV+ T +G++ A+C N MP++R LY ++
Sbjct: 28 HRKLMPTSLERCIWGQGDG---STIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELY 84
Query: 252 VLPFVDRS 229
P D S
Sbjct: 85 CAPTADGS 92
>At3g44310.1 68416.m04758 nitrilase 1 (NIT1) identical to SP|P32961
Nitrilase 1 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 346
Score = 29.5 bits (63), Expect = 1.2
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -2
Query: 426 HRKPERSCPCRCYYKSVPGRRDSYRPVHPTLVGRMMCAVCRRN-MPVFR-FLYENQWSVF 253
HRK + RC + G S PV+ T +G++ A+C N MP++R LY ++
Sbjct: 150 HRKLMPTSLERCIWGQGDG---STIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELY 206
Query: 252 VLPFVDRS 229
P D S
Sbjct: 207 CAPTADGS 214
>At3g44300.1 68416.m04757 nitrilase 2 (NIT2) identical to SP|P32962
Nitrilase 2 (EC 3.5.5.1) {Arabidopsis thaliana}
Length = 339
Score = 29.5 bits (63), Expect = 1.2
Identities = 22/68 (32%), Positives = 33/68 (48%), Gaps = 2/68 (2%)
Frame = -2
Query: 426 HRKPERSCPCRCYYKSVPGRRDSYRPVHPTLVGRMMCAVCRRN-MPVFR-FLYENQWSVF 253
HRK + RC + G S PV+ T +G++ A+C N MP++R LY ++
Sbjct: 143 HRKLMPTSLERCIWGQGDG---STIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIELY 199
Query: 252 VLPFVDRS 229
P D S
Sbjct: 200 CAPTADGS 207
>At3g60140.1 68416.m06715 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to Cyanogenic Beta-Glucosidase
(GI:1311386)(pdb:1CBG) [Trifolium Repens]; identical
beta-glucosidase GI:10834547
Length = 577
Score = 29.1 bits (62), Expect = 1.6
Identities = 13/24 (54%), Positives = 15/24 (62%), Gaps = 1/24 (4%)
Frame = +3
Query: 270 SHKETEKRACSFDTPHTSF-CLQG 338
S ++ E CSFD PHT F LQG
Sbjct: 546 SRRDEENNRCSFDFPHTHFGVLQG 569
>At3g10500.1 68416.m01260 no apical meristem (NAM) family protein
similar to to NAC2 (GI:645671) [Arabidopsis thaliana];
contains Pfam PF02365: No apical meristem (NAM) protein
Length = 549
Score = 29.1 bits (62), Expect = 1.6
Identities = 13/57 (22%), Positives = 28/57 (49%)
Frame = +3
Query: 132 SFSEEYLKPEQLYASTMLHGSNSEFFQELDQLCSDPQREEQTRTIDSHKETEKRACS 302
+F +E P QL + + E FQE++ + + +++ + KE ++ +CS
Sbjct: 362 TFFDETFDPSQLMGNEDVFFDQEELFQEVETKELEKEETSRSKHVVEEKEKDEASCS 418
>At2g24370.1 68415.m02912 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 816
Score = 27.9 bits (59), Expect = 3.7
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +3
Query: 150 LKPEQLYASTMLHGSNSEFFQELDQLCSDPQ 242
L+P L S LH S+++ Q+ +C DP+
Sbjct: 55 LRPSPLNNSASLHASSAKLSQDSSLVCRDPE 85
>At5g35730.1 68418.m04274 EXS family protein / ERD1/XPR1/SYG1 family
protein low similarity to xenotropic and polytropic
murine leukemia virus receptor [Mus spretus] GI:6093318;
contains Pfam profile PF03124: EXS family
Length = 457
Score = 27.5 bits (58), Expect = 4.9
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = +1
Query: 310 HRTHHSAYKGGMYRTITIPTARHAFI 387
H TH +K M+ TI +PT+ A++
Sbjct: 134 HLTHREMWKCSMWMTIIVPTSMTAYL 159
>At5g25040.1 68418.m02967 integral membrane transporter family
protein similar to biopterin transporter (GI:3377706)
[Leishmania mexicana]; contains Pfam PF03092: BT1
family; contains TIGRFAMS TIGR00788: folate/biopterin
transporter; Interpro IPR001991/ PR00173
Sodium:dicarboxylater symporter family
Length = 492
Score = 27.5 bits (58), Expect = 4.9
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -3
Query: 320 CVRCVEGTCPFFGFFMRINGPCLFFPLWIGAQLIQFLK 207
C +EGT FF M I+ L W+G +++ LK
Sbjct: 388 CPHGIEGT--FFALLMSIDNAGLMTSSWLGGKMLHVLK 423
>At4g16270.1 68417.m02468 peroxidase 40 (PER40) (P40) identical to
SP|O23474 Peroxidase 40 precursor (EC 1.11.1.7) (Atperox
P40) {Arabidopsis thaliana}
Length = 362
Score = 27.1 bits (57), Expect = 6.5
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = +3
Query: 186 HGSNSEFFQELDQLCS 233
HG N EF + L QLCS
Sbjct: 256 HGDNLEFLESLQQLCS 271
>At1g77860.1 68414.m09074 rhomboid family protein contains PFAM
domain PF01694, Rhomboid family
Length = 319
Score = 27.1 bits (57), Expect = 6.5
Identities = 10/34 (29%), Positives = 20/34 (58%)
Frame = -3
Query: 437 KSEPIESRRDRVHAGVTIKACLAVGIVIVLYIPP 336
+S ++ + DR HAG +I +A+G+ ++ P
Sbjct: 279 RSTRLKEQFDRPHAGESISTVIAIGVAMLTVFQP 312
>At1g50410.1 68414.m05650 SNF2 domain-containing protein / helicase
domain-containing protein / RING finger
domain-containing protein similar to transcription
factor RUSH-1alpha [Oryctolagus cuniculus] GI:1655930;
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 981
Score = 26.6 bits (56), Expect = 8.6
Identities = 14/43 (32%), Positives = 21/43 (48%), Gaps = 3/43 (6%)
Frame = +3
Query: 249 EQTRTIDSHKETEKRACSFDTPHTSFCLQGWDVQ---DDNYPY 368
++ +TI +H+ RAC +CL G +Q DD Y Y
Sbjct: 477 DEAQTIKNHRTQVARACCGLRAKRRWCLSGTPIQNTIDDLYSY 519
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,647,732
Number of Sequences: 28952
Number of extensions: 229878
Number of successful extensions: 731
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 718
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 731
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 811731120
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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