Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0585
(725 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 116 1e-26
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 105 2e-23
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 101 4e-22
At1g64030.1 68414.m07252 serpin family protein / serine protease... 89 4e-18
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 83 1e-16
At2g14540.1 68415.m01628 serpin family protein / serine protease... 81 1e-15
At2g35580.1 68415.m04357 serpin family protein / serine protease... 71 8e-13
At1g62170.1 68414.m07013 serpin family protein / serine protease... 67 1e-11
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 64 7e-11
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 63 2e-10
At3g15970.1 68416.m02019 Ran-binding protein 1 domain-containing... 29 4.1
At3g07525.2 68416.m00898 autophagocytosis-associated family prot... 28 5.5
At3g07525.1 68416.m00897 autophagocytosis-associated family prot... 28 5.5
At5g46290.1 68418.m05698 3-oxoacyl-[acyl-carrier-protein] syntha... 27 9.6
At1g31160.1 68414.m03812 zinc-binding protein, putative / protei... 27 9.6
>At2g25240.1 68415.m03020 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 324
Score = 116 bits (280), Expect = 1e-26
Identities = 78/249 (31%), Positives = 126/249 (50%), Gaps = 11/249 (4%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSS--ATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSK 179
+N W E +TN IK +++ DS+ + ++ VL NA+YFKGAWSSKFD +T DF++
Sbjct: 73 VNTWAEVHTNGLIKQILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHLLD 132
Query: 180 DKTIKVPMMYKRGDYKYGESAALNAQLIEIPYKGDQS--SLIVVLPKDKDGITQLQEALK 353
++KVP M D +Y S +++ +PY DQ S+ + LP DK+G+ L E +
Sbjct: 133 GTSVKVPFMTNYED-QYLRSYD-GFKVLRLPYIEDQRQFSMYIYLPNDKEGLAPLLEKIG 190
Query: 354 DPKTLETAQQSMYSTEVDLY-LPKFKIETETNLKDVLSNMNVNKIFNNDAQITRLL---- 518
+ ++ V + +PKFK E N +VL +M + FNN +T ++
Sbjct: 191 SEPSFFDNHIPLHCISVGAFRIPKFKFSFEFNASEVLKDMGLTSPFNNGGGLTEMVDSPS 250
Query: 519 KGESLSVSEAIQKXXXXXXXXXXXXXXXXXXTMTRSSKVYVRPPIVFNANKPFYYALQVD 698
G+ L VS + K ++ +S + R P F A++PF + ++ D
Sbjct: 251 NGDDLYVSSILHKACIEVDEEGTEAAAVSVGVVSCTS--FRRNP-DFVADRPFLFTVRED 307
Query: 699 --GVIMFNG 719
GVI+F G
Sbjct: 308 KSGVILFMG 316
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 105 bits (253), Expect = 2e-23
Identities = 76/253 (30%), Positives = 118/253 (46%), Gaps = 15/253 (5%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSA-----TAAVLVNAIYFKGAWSSKFDERLTSDRDFY 170
+N W + +TN IK +++ D + + +L NA+YFK AWS KFD +LT D DF+
Sbjct: 135 VNIWADVHTNGLIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDNDFH 194
Query: 171 VSKDKTIKVP-MMYKRGDYKYGESAALNAQLIEIPYKGDQS--SLIVVLPKDKDGITQLQ 341
+ T+KVP MM + Y G Q++ +PY D+ S+ + LP DKDG+ L
Sbjct: 195 LLDGNTVKVPFMMSYKDQYLRGYD---GFQVLRLPYVEDKRHFSMYIYLPNDKDGLAALL 251
Query: 342 EALKDPKTLETAQQSMYSTEVD-LYLPKFKIETETNLKDVLSNMNVNKIFNNDAQITRLL 518
E + + ++ T VD L +PK E +VL +M + F + +T ++
Sbjct: 252 EKISTEPGFLDSHIPLHRTPVDALRIPKLNFSFEFKASEVLKDMGLTSPFTSKGNLTEMV 311
Query: 519 ----KGESLSVSEAIQKXXXXXXXXXXXXXXXXXXTMTRSSKVYVRPPIVFNANKPFYYA 686
G+ L VS I K M + +R P F A+ PF +
Sbjct: 312 DSPSNGDKLHVSSIIHKACIEVDEEGTEAAAVSVAIM--MPQCLMRNP-DFVADHPFLFT 368
Query: 687 LQVD--GVIMFNG 719
++ D GVI+F G
Sbjct: 369 VREDNSGVILFIG 381
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 101 bits (243), Expect = 4e-22
Identities = 59/183 (32%), Positives = 98/183 (53%), Gaps = 7/183 (3%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLV--NAIYFKGAWSSKFDERLTSDRDFYVSK 179
+N W E +TN IK++++ DS+ + ++L+ NA+YFKGAWS KFD +LT DF++
Sbjct: 138 VNAWAEVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTKSYDFHLLD 197
Query: 180 DKTIKVPMM--YKRGDYKYGESAALNAQLIEIPYKGDQS--SLIVVLPKDKDGI-TQLQE 344
+KVP M YK+ +Y + +++ +PY DQ ++ + LP D+DG+ T L+E
Sbjct: 198 GTMVKVPFMTNYKKQYLEYYD----GFKVLRLPYVEDQRQFAMYIYLPNDRDGLPTLLEE 253
Query: 345 ALKDPKTLETAQQSMYSTEVDLYLPKFKIETETNLKDVLSNMNVNKIFNNDAQITRLLKG 524
P+ L+ +PKFK E DVL M + F + +T +++
Sbjct: 254 ISSKPRFLDNHIPRQRILTEAFKIPKFKFSFEFKASDVLKEMGLTLPFTH-GSLTEMVES 312
Query: 525 ESL 533
S+
Sbjct: 313 PSI 315
>At1g64030.1 68414.m07252 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311, serpin [Triticum
aestivum] GI:871551; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 385
Score = 88.6 bits (210), Expect = 4e-18
Identities = 53/168 (31%), Positives = 85/168 (50%), Gaps = 8/168 (4%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 185
+N WVE +TNN IKDL+ S++S T + NA+ FKGAW F++ T D DFY+
Sbjct: 139 VNSWVEHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYLVNGT 198
Query: 186 TIKVPMMYKRGDYKYGESAALNA-QLIEIPY-KGDQS-----SLIVVLPKDKDGITQLQE 344
++ VP M Y+ A + +++ +PY +G S+ LP KDG+ L E
Sbjct: 199 SVSVPFM---SSYENQYVRAYDGFKVLRLPYQRGSDDTNRKFSMYFYLPDKKDGLDDLLE 255
Query: 345 ALKDPKTLETAQQSMYSTEVDLY-LPKFKIETETNLKDVLSNMNVNKI 485
+ + Y E++ + +PKFKIE ++ VL + + +
Sbjct: 256 KMASTPGFLDSHIPTYRDELEKFRIPKFKIEFGFSVTSVLDRLGLRSM 303
>At1g47710.1 68414.m05302 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 391
Score = 83.4 bits (197), Expect = 1e-16
Identities = 61/249 (24%), Positives = 108/249 (43%), Gaps = 11/249 (4%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 185
+N W E+ TN I +++ S S T + NA+YFKG W+ KFDE LT + +F++
Sbjct: 138 VNSWAEKETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGN 197
Query: 186 TIKVPMMYKRGDYKYGESAALNAQLIEIPY-KGD---QSSLIVVLPKDKDGITQ-LQEAL 350
+ P M + K SA +++ +PY +G Q S+ LP +G++ L + +
Sbjct: 198 KVTAPFMTSK--KKQYVSAYDGFKVLGLPYLQGQDKRQFSMYFYLPDANNGLSDLLDKIV 255
Query: 351 KDPKTLETAQQSMYSTEVDLYLPKFKIETETNLKDVLSNMNVNKIFNNDAQITRLLK--- 521
P L+ + +PKFK + +VL + + F+ + +T +++
Sbjct: 256 STPGFLDNHIPRRQVKVREFKIPKFKFSFGFDASNVLKGLGLTSPFSGEEGLTEMVESPE 315
Query: 522 -GESLSVSEAIQKXXXXXXXXXXXXXXXXXXTMTRSSKVYVRPPIVFNANKPFYYAL--Q 692
G++L VS K + + I F A+ PF +
Sbjct: 316 MGKNLCVSNIFHKACIEVNEEGTEAAAASAGVIKLRGLLMEEDEIDFVADHPFLLVVTEN 375
Query: 693 VDGVIMFNG 719
+ GV++F G
Sbjct: 376 ITGVVLFIG 384
>At2g14540.1 68415.m01628 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 407
Score = 80.6 bits (190), Expect = 1e-15
Identities = 50/174 (28%), Positives = 84/174 (48%), Gaps = 7/174 (4%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 185
+N W +TN+ IK+++ S++S T + NA+YFKGAW FD+ +T D+ F++ K
Sbjct: 167 VNTWASRHTNDLIKEILPRGSVTSLTNWIYGNALYFKGAWEKAFDKSMTRDKPFHLLNGK 226
Query: 186 TIKVPMMYKRGDYKYGESAALNAQLIEIPYK------GDQSSLIVVLPKDKDGITQLQEA 347
++ VP M R K A +++ +PY+ + S+ + LP K + L E
Sbjct: 227 SVSVPFM--RSYEKQFIEAYDGFKVLRLPYRQGRDDTNREFSMYLYLPDKKGELDNLLER 284
Query: 348 LKDPKTLETAQQSMYSTEV-DLYLPKFKIETETNLKDVLSNMNVNKIFNNDAQI 506
+ + Y +V D +PKFKIE V ++ +N + A I
Sbjct: 285 ITSNPGFLDSHIPEYRVDVGDFRIPKFKIEFGFEASSVFNDFELNVSLHQKALI 338
>At2g35580.1 68415.m04357 serpin family protein / serine protease
inhibitor family protein similar to protein zx [Hordeum
vulgare subsp. vulgare] GI:19071, serpin [Triticum
aestivum] GI:1885350; contains Pfam profile PF00079:
Serpin (serine protease inhibitor)
Length = 374
Score = 70.9 bits (166), Expect = 8e-13
Identities = 46/165 (27%), Positives = 75/165 (45%), Gaps = 8/165 (4%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLV--NPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSK 179
+N WVE+ TN I +L+ NP S + T + NA++F G W S+FD LT D DF++
Sbjct: 138 VNSWVEKQTNGLITNLLPSNPKS-APLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLD 196
Query: 180 DKTIKVPMMYK---RGDYKYGESAALNAQLIEIPYKGDQSSLIVVLPKDKDGITQLQEAL 350
++VP M R + Y +N Q S+ + LP +KDG+ + E L
Sbjct: 197 GTKVRVPFMTGASCRYTHVYEGFKVINLQYRRGREDSRSFSMQIYLPDEKDGLPSMLERL 256
Query: 351 KDPKTLETAQQSMYSTEV---DLYLPKFKIETETNLKDVLSNMNV 476
+ + + S +L +P+FK + + L +
Sbjct: 257 ASTRGFLKDNEVLPSHSAVIKELKIPRFKFDFAFEASEALKGFGL 301
>At1g62170.1 68414.m07013 serpin family protein / serine protease
inhibitor family protein similar to phloem serpin-1
GI:9937311 from [Cucurbita maxima]; contains Pfam
profile PF00079: Serpin (serine protease inhibitor)
Length = 433
Score = 67.3 bits (157), Expect = 1e-11
Identities = 44/169 (26%), Positives = 78/169 (46%), Gaps = 8/169 (4%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 185
+N W +TN IKDL+ S++S T V +A+YFKG W K+ + +T + FY+
Sbjct: 203 VNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKSMTKCKPFYLLNGT 262
Query: 186 TIKVPMMYKRGDYKYGESAALNA-QLIEIPYKGDQS------SLIVVLPKDKDGITQLQE 344
++ VP M ++ AA + +++ +PY+ + ++ + LP K + L E
Sbjct: 263 SVSVPFM---SSFEKQYIAAYDGFKVLRLPYRQGRDNTNRNFAMYIYLPDKKGELDDLLE 319
Query: 345 AL-KDPKTLETAQQSMYSTEVDLYLPKFKIETETNLKDVLSNMNVNKIF 488
+ P L++ +PKFKIE S+ ++ F
Sbjct: 320 RMTSTPGFLDSHNPERRVKVGKFRIPKFKIEFGFEASSAFSDFELDVSF 368
>At1g51330.1 68414.m05772 serpin-related / serine protease
inhibitor-related similar to serpin [Hordeum vulgare
subsp. vulgare] CAA64599.1 GI:1197577
Length = 193
Score = 64.5 bits (150), Expect = 7e-11
Identities = 29/75 (38%), Positives = 46/75 (61%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 185
+N W +TN IK+L+ P S+++ T + NA+YFKGAW +KF + +T + F++ K
Sbjct: 39 VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNGK 98
Query: 186 TIKVPMMYKRGDYKY 230
+ VP M K + KY
Sbjct: 99 QVLVPFM-KSYERKY 112
>At1g63280.1 68414.m07154 serpin-related / serine protease
inhibitor-related similar to protein zx [Hordeum vulgare
subsp. vulgare] GI:19071, serpin [Triticum aestivum]
GI:1885346
Length = 120
Score = 63.3 bits (147), Expect = 2e-10
Identities = 29/67 (43%), Positives = 41/67 (61%)
Frame = +3
Query: 6 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 185
+N W ++TN I DL+ S+ S T V NA+YFKGAW +KFD+ T D +F+ K+
Sbjct: 16 LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFHQGKE- 74
Query: 186 TIKVPMM 206
+ VP M
Sbjct: 75 -VHVPFM 80
>At3g15970.1 68416.m02019 Ran-binding protein 1 domain-containing
protein / RanBP1 domain-containing protein similar to
Ran binding protein [Homo sapiens] GI:624232; contains
Pfam profile PF00638: RanBP1 domain
Length = 465
Score = 28.7 bits (61), Expect = 4.1
Identities = 23/91 (25%), Positives = 42/91 (46%), Gaps = 3/91 (3%)
Frame = +3
Query: 108 YFKGAWSSKFDERLTSDRDFYVSKDKTIKVPM-MYKRGDYKYGESAAL--NAQLIEIPYK 278
Y +G W ER + +S + K + M +G+Y+ +A+L +L ++ K
Sbjct: 344 YLEGGWK----ERGKGELKVNISTTENRKARLVMRSKGNYRLTLNASLYPEMKLAKMDKK 399
Query: 279 GDQSSLIVVLPKDKDGITQLQEALKDPKTLE 371
G + + + KDG++ L KDP +E
Sbjct: 400 GITFACVNSVSDAKDGLSTLALKFKDPTVVE 430
>At3g07525.2 68416.m00898 autophagocytosis-associated family protein
contains autophagocytosis associated protein C-terminal
domain, Pfam:PF03987
Length = 226
Score = 28.3 bits (60), Expect = 5.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 162 DFYVSKDKTIKVPMMYKRGDYKYGESAALNAQLIEIP 272
DF++ + KVP++Y RG GE AL+ ++P
Sbjct: 110 DFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVP 146
>At3g07525.1 68416.m00897 autophagocytosis-associated family protein
contains autophagocytosis associated protein C-terminal
domain, Pfam:PF03987
Length = 225
Score = 28.3 bits (60), Expect = 5.5
Identities = 13/37 (35%), Positives = 21/37 (56%)
Frame = +3
Query: 162 DFYVSKDKTIKVPMMYKRGDYKYGESAALNAQLIEIP 272
DF++ + KVP++Y RG GE AL+ ++P
Sbjct: 109 DFHIVYSASYKVPVLYFRGYCSGGEPLALDVIKKDVP 145
>At5g46290.1 68418.m05698 3-oxoacyl-[acyl-carrier-protein] synthase
I identical to Swiss-Prot:P52410
3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplast
precursor (EC 2.3.1.41) (Beta-ketoacyl-ACP synthase I)
(KAS I) [Arabidopsis thaliana]
Length = 473
Score = 27.5 bits (58), Expect = 9.6
Identities = 19/68 (27%), Positives = 31/68 (45%), Gaps = 2/68 (2%)
Frame = +3
Query: 333 QLQEALKDPKTLETAQQSMYSTEVDLYLPKFKIETETNLKDVLSNMNVNKIFNN--DAQI 506
Q + + + + L Q+S S K ET+ + V++ M + +F N DA
Sbjct: 24 QSHQLITNARPLRRQQRSFISASASTVSAP-KRETDPKKRVVITGMGLVSVFGNDVDAYY 82
Query: 507 TRLLKGES 530
+LL GES
Sbjct: 83 EKLLSGES 90
>At1g31160.1 68414.m03812 zinc-binding protein, putative / protein
kinase C inhibitor, putative similar to 14 kDa
zinc-binding protein (Protein kinase C inhibitor, PKCI)
[Zea mays] Swiss-Prot:P42856
Length = 187
Score = 27.5 bits (58), Expect = 9.6
Identities = 11/36 (30%), Positives = 26/36 (72%)
Frame = +3
Query: 297 IVVLPKDKDGITQLQEALKDPKTLETAQQSMYSTEV 404
++V+PK +DG+T L +A +P+ +E Q ++++++
Sbjct: 111 VLVIPKLRDGLTSLGKA--EPRHVEVLGQLLHASKI 144
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,422,532
Number of Sequences: 28952
Number of extensions: 244673
Number of successful extensions: 656
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 613
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 646
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1584903024
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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