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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0548
         (372 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g17750.1 68416.m02265 protein kinase family protein contains ...    31   0.24 
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi...    27   3.0  
At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl...    26   7.0  

>At3g17750.1 68416.m02265 protein kinase family protein contains
           Pfam profile: PF00069 Eukaryotic protein kinase domain
          Length = 1138

 Score = 31.1 bits (67), Expect = 0.24
 Identities = 12/23 (52%), Positives = 18/23 (78%)
 Frame = -3

Query: 157 IDKRLINTKTTFLVGTYLLIPQG 89
           +D++LINT   FL+G+YL +P G
Sbjct: 357 LDEKLINTDNAFLLGSYLDVPIG 379


>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
           to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
           {Saccharomyces cerevisiae}; contains Pfam profiles
           PF00240: Ubiquitin family, PF00632: HECT-domain
           (ubiquitin-transferase)
          Length = 873

 Score = 27.5 bits (58), Expect = 3.0
 Identities = 12/33 (36%), Positives = 17/33 (51%)
 Frame = +2

Query: 2   KSTIKRIIEAILSTAAI*PKTRTSYCLPSPLRY 100
           KS  K  ++  LS     PK + +YCLP  L +
Sbjct: 247 KSCAKSSVKLFLSNCVALPKNQKNYCLPIVLEF 279


>At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl
           CoA:diacylglycerol acyltransferase (DGAT) identical to
           gi:5050913, gi:6625553
          Length = 520

 Score = 26.2 bits (55), Expect = 7.0
 Identities = 16/68 (23%), Positives = 36/68 (52%)
 Frame = +1

Query: 166 LHPHLKTPLKSFLYLMKIVT*VLHYLYVKLKFQNRY*YNKSKLIN*LIVSFKTV*QVYDI 345
           L  ++  P+  FL+++  +T VL+ +YV L+  + +    + ++   IV  K V   +  
Sbjct: 200 LQKYISEPVVIFLHIIITMTEVLYPVYVTLRCDSAFLSGVTLMLLTCIVWLKLVSYAHTS 259

Query: 346 FTLSSLSS 369
           + + SL++
Sbjct: 260 YDIRSLAN 267


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,162,632
Number of Sequences: 28952
Number of extensions: 99726
Number of successful extensions: 109
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 497853200
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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