BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0548
(372 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17750.1 68416.m02265 protein kinase family protein contains ... 31 0.24
At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative simi... 27 3.0
At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl... 26 7.0
>At3g17750.1 68416.m02265 protein kinase family protein contains
Pfam profile: PF00069 Eukaryotic protein kinase domain
Length = 1138
Score = 31.1 bits (67), Expect = 0.24
Identities = 12/23 (52%), Positives = 18/23 (78%)
Frame = -3
Query: 157 IDKRLINTKTTFLVGTYLLIPQG 89
+D++LINT FL+G+YL +P G
Sbjct: 357 LDEKLINTDNAFLLGSYLDVPIG 379
>At4g12570.1 68417.m01983 ubiquitin-protein ligase, putative similar
to SP|P39940 Ubiquitin--protein ligase RSP5 (EC 6.3.2.-)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF00240: Ubiquitin family, PF00632: HECT-domain
(ubiquitin-transferase)
Length = 873
Score = 27.5 bits (58), Expect = 3.0
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +2
Query: 2 KSTIKRIIEAILSTAAI*PKTRTSYCLPSPLRY 100
KS K ++ LS PK + +YCLP L +
Sbjct: 247 KSCAKSSVKLFLSNCVALPKNQKNYCLPIVLEF 279
>At2g19450.1 68415.m02272 diacylglycerol O-acyltransferase / acyl
CoA:diacylglycerol acyltransferase (DGAT) identical to
gi:5050913, gi:6625553
Length = 520
Score = 26.2 bits (55), Expect = 7.0
Identities = 16/68 (23%), Positives = 36/68 (52%)
Frame = +1
Query: 166 LHPHLKTPLKSFLYLMKIVT*VLHYLYVKLKFQNRY*YNKSKLIN*LIVSFKTV*QVYDI 345
L ++ P+ FL+++ +T VL+ +YV L+ + + + ++ IV K V +
Sbjct: 200 LQKYISEPVVIFLHIIITMTEVLYPVYVTLRCDSAFLSGVTLMLLTCIVWLKLVSYAHTS 259
Query: 346 FTLSSLSS 369
+ + SL++
Sbjct: 260 YDIRSLAN 267
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,162,632
Number of Sequences: 28952
Number of extensions: 99726
Number of successful extensions: 109
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 109
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 109
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 497853200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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