Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0496
(352 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g52280.1 68418.m06488 protein transport protein-related low s... 29 0.87
At2g41440.1 68415.m05120 expressed protein 29 0.87
At5g55180.1 68418.m06879 glycosyl hydrolase family 17 protein si... 27 3.5
At5g59400.1 68418.m07443 expressed protein predicted protein, Ar... 26 6.1
At5g35380.1 68418.m04205 protein kinase family protein contains ... 26 6.1
At5g11140.1 68418.m01302 hypothetical protein 26 6.1
At5g07650.1 68418.m00876 formin homology 2 domain-containing pro... 26 8.1
At4g26200.1 68417.m03772 1-aminocyclopropane-1-carboxylate synth... 26 8.1
At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containi... 26 8.1
At4g08170.2 68417.m01350 inositol 1,3,4-trisphosphate 5/6-kinase... 26 8.1
At4g08170.1 68417.m01349 inositol 1,3,4-trisphosphate 5/6-kinase... 26 8.1
At3g24170.1 68416.m03034 glutathione reductase, putative identic... 26 8.1
At3g20540.1 68416.m02600 DNA-directed DNA polymerase family prot... 26 8.1
>At5g52280.1 68418.m06488 protein transport protein-related low
similarity to SP|P25386 Intracellular protein transport
protein USO1 {Saccharomyces cerevisiae}
Length = 853
Score = 29.1 bits (62), Expect = 0.87
Identities = 21/59 (35%), Positives = 33/59 (55%), Gaps = 6/59 (10%)
Frame = +2
Query: 83 ADDKYTDKYDKIN-LQ---EILENKRLLES--YMDCVLGKGKCTPEGKELKDHLQEALQ 241
A+D+Y D D I+ L+ EILE K +S Y +C++ + + KELK L++ Q
Sbjct: 484 AEDEYLDSKDIIDELKSQIEILEGKLKQQSLEYSECLITVNELESQVKELKKELEDQAQ 542
>At2g41440.1 68415.m05120 expressed protein
Length = 544
Score = 29.1 bits (62), Expect = 0.87
Identities = 16/44 (36%), Positives = 23/44 (52%)
Frame = +2
Query: 107 YDKINLQEILENKRLLESYMDCVLGKGKCTPEGKELKDHLQEAL 238
++ ++++ L+ RLL M C G PE LK HLQ AL
Sbjct: 392 HEAVSMENELKRLRLLTRRMTCKDLDGLTFPELLSLKSHLQTAL 435
>At5g55180.1 68418.m06879 glycosyl hydrolase family 17 protein
similar to elicitor inducible chitinase Nt-SubE76
GI:11071974 from [Nicotiana tabacum]
Length = 460
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/38 (31%), Positives = 18/38 (47%)
Frame = +2
Query: 137 ENKRLLESYMDCVLGKGKCTPEGKELKDHLQEALQTGC 250
+NK + ++G+ C GK K+ LQE L C
Sbjct: 355 DNKEKVVPVKPSLVGQTWCVANGKTTKEKLQEGLDYAC 392
>At5g59400.1 68418.m07443 expressed protein predicted protein,
Arabidopsis thaliana
Length = 299
Score = 26.2 bits (55), Expect = 6.1
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = -3
Query: 113 CHICQCTYHQQVRPRVHSSKL*GPSWL 33
CH+C+CT + + +S+L G W+
Sbjct: 255 CHVCECTLEFRTKVEKSASRL-GRKWV 280
>At5g35380.1 68418.m04205 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 731
Score = 26.2 bits (55), Expect = 6.1
Identities = 12/39 (30%), Positives = 25/39 (64%)
Frame = +2
Query: 197 PEGKELKDHLQEALQTGCEKCTEAQ*KGAETSIDYLINN 313
PE K+L+D +E+++ G + + + G+ TSI +I++
Sbjct: 675 PELKKLRDLAEESIKFGVRQPSPIRSSGSATSIQEIISD 713
>At5g11140.1 68418.m01302 hypothetical protein
Length = 241
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/63 (25%), Positives = 31/63 (49%)
Frame = +2
Query: 56 LSCVLVAVLADDKYTDKYDKINLQEILENKRLLESYMDCVLGKGKCTPEGKELKDHLQEA 235
L VL VL +Y D+ + +++ RLL + + L K + KE+K+ ++
Sbjct: 141 LRSVLEEVLEATRYFDRCEMAVMEKKAGEHRLLLAKQEMELSLKKLAEKEKEMKEFREKL 200
Query: 236 LQT 244
++T
Sbjct: 201 MKT 203
>At5g07650.1 68418.m00876 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02128
Length = 815
Score = 25.8 bits (54), Expect = 8.1
Identities = 24/70 (34%), Positives = 33/70 (47%), Gaps = 7/70 (10%)
Frame = +2
Query: 41 KVLIVLSCVLVAVLADDKYTDKYDKI-NLQEIL---ENKRLLESYMDCVLGKGKCTPEGK 208
KV I L+ ++ AVL D+Y D+I NL E LL++Y GKC K
Sbjct: 484 KVNIPLADMMAAVLGMDEYVLDVDQIENLIRFCPTKEEMELLKNYTGDKATLGKCEQLAK 543
Query: 209 ---ELKDHLQ 229
LK+H +
Sbjct: 544 AKAPLKEHFR 553
>At4g26200.1 68417.m03772 1-aminocyclopropane-1-carboxylate
synthase, putative / ACC synthase, putative similar to
ACC synthase from Malus x domestica, GI:1658062
[U73816], Pyrus pyrifolia GI:4586411
Length = 447
Score = 25.8 bits (54), Expect = 8.1
Identities = 8/23 (34%), Positives = 19/23 (82%)
Frame = +2
Query: 65 VLVAVLADDKYTDKYDKINLQEI 133
+L ++L+D+++T+KY +IN + +
Sbjct: 324 MLASMLSDEEFTEKYIRINRERL 346
>At4g25270.1 68417.m03635 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 527
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/33 (33%), Positives = 23/33 (69%)
Frame = +3
Query: 234 LYRLVARNALKPNKRALKLVLII*SIMNWKFGR 332
++RL+ +N ++P+K A+ VL ++++K GR
Sbjct: 283 IFRLMVQNGIEPDKVAISSVLA--RVLSFKHGR 313
>At4g08170.2 68417.m01350 inositol 1,3,4-trisphosphate 5/6-kinase
family protein similar to inositol phosphate kinase
(GI:27549256) [Zea mays]; similar to inositol
1,3,4-trisphosphate 5/6-kinase (GI:3396079) [Arabidopsis
thaliana]
Length = 353
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/30 (40%), Positives = 21/30 (70%)
Frame = -1
Query: 193 TLALAEHAIHIRLQEPLVLQNFLQVNFVIF 104
+LA +H++ ++L+ PLVLQ F+ V+F
Sbjct: 191 SLAYDQHSL-LKLEPPLVLQEFVNHGGVLF 219
>At4g08170.1 68417.m01349 inositol 1,3,4-trisphosphate 5/6-kinase
family protein similar to inositol phosphate kinase
(GI:27549256) [Zea mays]; similar to inositol
1,3,4-trisphosphate 5/6-kinase (GI:3396079) [Arabidopsis
thaliana]
Length = 265
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/30 (40%), Positives = 21/30 (70%)
Frame = -1
Query: 193 TLALAEHAIHIRLQEPLVLQNFLQVNFVIF 104
+LA +H++ ++L+ PLVLQ F+ V+F
Sbjct: 103 SLAYDQHSL-LKLEPPLVLQEFVNHGGVLF 131
>At3g24170.1 68416.m03034 glutathione reductase, putative identical
to GB:P48641 from [Arabidopsis thaliana]
Length = 499
Score = 25.8 bits (54), Expect = 8.1
Identities = 13/44 (29%), Positives = 21/44 (47%)
Frame = +2
Query: 125 QEILENKRLLESYMDCVLGKGKCTPEGKELKDHLQEALQTGCEK 256
QE K +++ D V+G C P+ E+ + AL+ G K
Sbjct: 419 QEKTLMKLIVDEKSDKVIGASMCGPDAAEIMQGIAIALKCGATK 462
>At3g20540.1 68416.m02600 DNA-directed DNA polymerase family protein
similar to PolI-like DNA polymerase [Oryza sativa]
GI:19912795; contains Pfam profiles PF01612: 3'-5'
exonuclease, PF00476: DNA polymerase I family A
Length = 1034
Score = 25.8 bits (54), Expect = 8.1
Identities = 12/49 (24%), Positives = 27/49 (55%), Gaps = 2/49 (4%)
Frame = +2
Query: 95 YTDKYDKINLQEILENKRLLESYMDCVLGKGKCTPE--GKELKDHLQEA 235
Y D+ + QE+ + + + + Y+ +LG+ + PE + K+H++ A
Sbjct: 896 YNDRQEVRKWQELRKKEAIQKGYVLTLLGRARKFPEYRSRAQKNHIERA 944
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,258,379
Number of Sequences: 28952
Number of extensions: 132044
Number of successful extensions: 380
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 369
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 380
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 439384704
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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