BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0461 (710 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC890.07c |rmt1|prmt1|type I protein arginine N-methyltransfer... 42 9e-05 SPBC8D2.10c |rmt3|rmt3|type I ribosomal protein arginine N-methy... 36 0.006 SPBC14C8.16c |bot1||mitochondrial ribosomal protein subunit S35 ... 27 2.6 SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subuni... 27 3.5 SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces p... 26 4.6 SPBC15D4.02 |||transcription factor, zf-fungal binuclear cluster... 26 6.1 SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |S... 25 8.1 SPBC1773.01 |||striatin homolog|Schizosaccharomyces pombe|chr 2|... 25 8.1 SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosacc... 25 8.1 >SPAC890.07c |rmt1|prmt1|type I protein arginine N-methyltransferase Rmt1|Schizosaccharomyces pombe|chr 1|||Manual Length = 339 Score = 41.9 bits (94), Expect = 9e-05 Identities = 32/147 (21%), Positives = 65/147 (44%) Frame = -2 Query: 604 WNNVHGVSMSAFAKHLRKNKSTKPEILNIEQKDLLGDEVALCWINLREDSASDLNEFSIQ 425 W++V+G S K + K +P + +++ + + + ++L+ DL FS Sbjct: 182 WDDVYGFDFSPIKKDVWK----EPLVDTVDRIAVNTNSCVILDLDLKTVKKEDL-AFSSP 236 Query: 424 HVVGAKKSGSYEGLCLWFECNFPSMSETKDENRIILDTSPNSLATHWKQTAIVLPQEVEV 245 + A ++ WF+ F + + I T P S THWKQT +++ V Sbjct: 237 FEITATRNDFVHAFLAWFDIEFSACHKP-----IKFSTGPFSRYTHWKQTVFYTHKDLTV 291 Query: 244 EEGEPIAFQLSMKRDTEHNRRYNLQMT 164 + GE I ++ K ++R ++ ++ Sbjct: 292 KAGEYIRGTITCKPAEGNHRELDIDIS 318 >SPBC8D2.10c |rmt3|rmt3|type I ribosomal protein arginine N-methytransferase Rmt3|Schizosaccharomyces pombe|chr 2|||Manual Length = 543 Score = 35.9 bits (79), Expect = 0.006 Identities = 31/151 (20%), Positives = 60/151 (39%), Gaps = 5/151 (3%) Frame = -2 Query: 604 WNNVHGVSMSAFAKHLRKNKSTKPEILNIEQKDLLGDEVALCWINLREDSASDLNEFSIQ 425 W++V+G M+ K S + + Q + V N+ D++ F+ Sbjct: 383 WSDVYGFKMNGMKDASYKGVSVQV----VPQTYVNAKPVVFARFNMHTCKVQDVS-FTSP 437 Query: 424 HVVGAKKSGSYEGLCLWFECNFPS-----MSETKDENRIILDTSPNSLATHWKQTAIVLP 260 + G LWF+ F + + E DE T P THWKQ ++L Sbjct: 438 FSLIIDNEGPLCAFTLWFDTYFTTKRTQPIPEAIDE-ACGFTTGPQGTPTHWKQCVLLLR 496 Query: 259 QEVEVEEGEPIAFQLSMKRDTEHNRRYNLQM 167 +++G + +S ++ ++NR ++ + Sbjct: 497 NRPFLQKGTRVEGTISFSKNKKNNRDLDISV 527 >SPBC14C8.16c |bot1||mitochondrial ribosomal protein subunit S35 |Schizosaccharomyces pombe|chr 2|||Manual Length = 315 Score = 27.1 bits (57), Expect = 2.6 Identities = 17/59 (28%), Positives = 26/59 (44%) Frame = -2 Query: 367 CNFPSMSETKDENRIILDTSPNSLATHWKQTAIVLPQEVEVEEGEPIAFQLSMKRDTEH 191 C P M + D N I L ++P SL + PQE P +L+M+++ H Sbjct: 199 CTKPEMMQFNDGNDIPLRSNPVSLWKSLPEGETFTPQEAAKILKWPSIEELNMRQNATH 257 >SPAC959.09c |apc5|SPAP32A8.01c|anaphase-promoting complex subunit Apc5 |Schizosaccharomyces pombe|chr 1|||Manual Length = 737 Score = 26.6 bits (56), Expect = 3.5 Identities = 20/79 (25%), Positives = 36/79 (45%), Gaps = 6/79 (7%) Frame = +3 Query: 354 LGKLHSNHRHKPS*EPDF----LAPTTCWMLNSFKS--DALSSLKLIQQSATSSPSKSFC 515 L ++H H PS ++ L W L+SF+ + SL + S ++ Sbjct: 69 LTRIHDITSHLPSSIEEYSVYSLLHERLWSLHSFEDIHEIFISLGNYIEGVYDSEEEAPH 128 Query: 516 SIFKISGLVDLFLRRCLAK 572 +F S L+ +FLR+C+ + Sbjct: 129 MLFSSSSLLSIFLRKCVVE 147 >SPBC1709.17 |||folylpolyglutamate synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 505 Score = 26.2 bits (55), Expect = 4.6 Identities = 11/28 (39%), Positives = 14/28 (50%) Frame = -2 Query: 478 WINLREDSASDLNEFSIQHVVGAKKSGS 395 W+ SDLN ++ HV G K GS Sbjct: 65 WLRCIGYQPSDLNRLNVIHVAGTKGKGS 92 >SPBC15D4.02 |||transcription factor, zf-fungal binuclear cluster type|Schizosaccharomyces pombe|chr 2|||Manual Length = 419 Score = 25.8 bits (54), Expect = 6.1 Identities = 12/41 (29%), Positives = 19/41 (46%) Frame = -2 Query: 385 LCLWFECNFPSMSETKDENRIILDTSPNSLATHWKQTAIVL 263 LCLW C+FP +S + I ++ LA ++ L Sbjct: 126 LCLWDACHFPPLSPYSSFSSIYWSSTVPELALRNPNISVAL 166 >SPBC691.04 |||mitochondrial ATP-dependent RNA helicase Mss116 |Schizosaccharomyces pombe|chr 2|||Manual Length = 535 Score = 25.4 bits (53), Expect = 8.1 Identities = 16/49 (32%), Positives = 24/49 (48%) Frame = +3 Query: 459 SSLKLIQQSATSSPSKSFCSIFKISGLVDLFLRRCLAKADIDTPCTLFH 605 SSL L+QQ +S+PS +VD F+ L + + PC + H Sbjct: 278 SSLSLLQQLTSSNPSSRIIVFLPTISMVD-FVGGVL-ENHLKIPCFILH 324 >SPBC1773.01 |||striatin homolog|Schizosaccharomyces pombe|chr 2|||Manual Length = 612 Score = 25.4 bits (53), Expect = 8.1 Identities = 13/48 (27%), Positives = 23/48 (47%) Frame = -2 Query: 346 ETKDENRIILDTSPNSLATHWKQTAIVLPQEVEVEEGEPIAFQLSMKR 203 E KD N I+L S + WK T L ++ + +P++ ++ R Sbjct: 401 EVKDNNPILLSLSSDGTVRGWKYTGEQL-FKIRCDSKQPLSMSVTDSR 447 >SPAC1A6.09c |lag1||sphingosine N-acyltransferase Lag1|Schizosaccharomyces pombe|chr 1|||Manual Length = 390 Score = 25.4 bits (53), Expect = 8.1 Identities = 15/50 (30%), Positives = 22/50 (44%) Frame = +3 Query: 144 TASGSSNVICKLYLRLCSVSLFMLSWKAIGSPSSTSTSCGNTIAVCFQCV 293 T SN + K +L LF+L W+ C + IA+CF C+ Sbjct: 43 TLEDPSNFVAKDGTKLVQAPLFLLVWQ--------KEICLSIIAICFACL 84 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,883,571 Number of Sequences: 5004 Number of extensions: 60763 Number of successful extensions: 186 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 182 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 186 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 331187010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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