Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0450
(764 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g34440.1 68417.m04894 protein kinase family protein contains ... 37 0.013
At4g13340.1 68417.m02084 leucine-rich repeat family protein / ex... 32 0.48
At1g49270.1 68414.m05524 protein kinase family protein contains ... 31 0.64
At2g43680.2 68415.m05430 calmodulin-binding family protein simil... 31 0.84
At2g43680.1 68415.m05429 calmodulin-binding family protein simil... 31 0.84
At1g35230.1 68414.m04369 arabinogalactan-protein (AGP5) identica... 31 1.1
At5g59170.1 68418.m07416 proline-rich family protein contains pr... 30 1.9
At1g70460.1 68414.m08107 protein kinase, putative contains Pfam ... 30 1.9
At1g26110.1 68414.m03186 expressed protein 29 2.6
At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family... 29 3.4
At1g60200.1 68414.m06781 splicing factor PWI domain-containing p... 29 4.5
At5g64370.1 68418.m08086 beta-ureidopropionase, putative / beta-... 28 5.9
At5g38560.1 68418.m04662 protein kinase family protein contains ... 28 5.9
At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) riboso... 28 5.9
At3g24550.1 68416.m03083 protein kinase family protein contains ... 28 5.9
At4g16140.1 68417.m02445 proline-rich family protein contains pr... 28 7.8
At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid t... 28 7.8
At1g54860.1 68414.m06263 expressed protein 28 7.8
At1g12090.1 68414.m01399 protease inhibitor/seed storage/lipid t... 28 7.8
>At4g34440.1 68417.m04894 protein kinase family protein contains
Pfam domain, PF00069: Protein kinase domain
Length = 670
Score = 37.1 bits (82), Expect = 0.013
Identities = 27/67 (40%), Positives = 31/67 (46%), Gaps = 5/67 (7%)
Frame = -1
Query: 362 SPMPST*CGPPPSASNDF-PPCALSRQSTGLEDAPRSLYPSVASIRGLSP----PPGVAG 198
SP PS+ PPP S F PP A Q T +P S P VA+ +P PP G
Sbjct: 38 SPPPSSISAPPPDISASFSPPPAPPTQETSPPTSPSSSPPVVANPSPQTPENPSPPAPEG 97
Query: 197 STDVYCP 177
ST V P
Sbjct: 98 STPVTPP 104
>At4g13340.1 68417.m02084 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 760
Score = 31.9 bits (69), Expect = 0.48
Identities = 21/72 (29%), Positives = 25/72 (34%), Gaps = 1/72 (1%)
Frame = -1
Query: 392 LYLPTAGHRPSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLY-PSVASIRGLSP 216
+Y P P P P PPP + PP S P +Y P + P
Sbjct: 463 VYSPPPPPPPPPPPPPVYSPPPPSPPPPPPPVYSPPPPPPPPPPPPVYSPPPPPVYSSPP 522
Query: 215 PPGVAGSTDVYC 180
PP T VYC
Sbjct: 523 PPPSPAPTPVYC 534
>At1g49270.1 68414.m05524 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 699
Score = 31.5 bits (68), Expect = 0.64
Identities = 17/52 (32%), Positives = 22/52 (42%)
Frame = -1
Query: 365 PSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPPP 210
P P S PPP + PP L S+ + PS +S + SPPP
Sbjct: 44 PPPPSSPDIAPPPQQQQESPPPPLPENSSDGSSSSSPPPPSDSSSQSQSPPP 95
>At2g43680.2 68415.m05430 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 669
Score = 31.1 bits (67), Expect = 0.84
Identities = 20/51 (39%), Positives = 24/51 (47%)
Frame = -1
Query: 365 PSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPP 213
PSP PS+ G P A + PP + T D PR P AS+R PP
Sbjct: 196 PSPRPSSPRGASPQAISSKPPSPRAEPPT--LDTPRPPSPRAASLRA-DPP 243
>At2g43680.1 68415.m05429 calmodulin-binding family protein similar
to SF16 protein [Helianthus annuus] GI:560150; contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 668
Score = 31.1 bits (67), Expect = 0.84
Identities = 20/51 (39%), Positives = 24/51 (47%)
Frame = -1
Query: 365 PSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPP 213
PSP PS+ G P A + PP + T D PR P AS+R PP
Sbjct: 195 PSPRPSSPRGASPQAISSKPPSPRAEPPT--LDTPRPPSPRAASLRA-DPP 242
>At1g35230.1 68414.m04369 arabinogalactan-protein (AGP5) identical
to gi_3883128_gb_AAC77827
Length = 133
Score = 30.7 bits (66), Expect = 1.1
Identities = 26/66 (39%), Positives = 30/66 (45%), Gaps = 4/66 (6%)
Frame = -1
Query: 383 PTAGHRP---SPMPST*CGPPPSASNDFPPCALSRQSTGLEDA-PRSLYPSVASIRGLSP 216
PT P +P PS PPPSA PP +S+ T A P S PS A G +
Sbjct: 37 PTPSQSPRATAPAPSPSANPPPSAPTTAPP--VSQPPTESPPAPPTSTSPSGAP--GTNV 92
Query: 215 PPGVAG 198
P G AG
Sbjct: 93 PSGEAG 98
>At5g59170.1 68418.m07416 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 288
Score = 29.9 bits (64), Expect = 1.9
Identities = 16/47 (34%), Positives = 21/47 (44%), Gaps = 2/47 (4%)
Frame = -2
Query: 349 PHNAVLRLPHPTTSRRAH*VVS--PPGWRTPHAPCTHPWPPFEDFPP 215
PH V + P P H + PP P P +PWPP + +PP
Sbjct: 226 PHPPV-KYPPPPYKTYPHPPIKTYPPPKECPPPPEHYPWPPKKKYPP 271
>At1g70460.1 68414.m08107 protein kinase, putative contains Pfam
PF00069: Protein kinase domain
Length = 710
Score = 29.9 bits (64), Expect = 1.9
Identities = 22/73 (30%), Positives = 24/73 (32%)
Frame = -1
Query: 383 PTAGHRPSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPPPGV 204
P P P P PPP A PP S T P L S L+PPP
Sbjct: 31 PPTDSAPPPSPPADSSPPP-ALPSLPPAVFSPPPTVSSPPPPPLDSSPPPPPDLTPPPSS 89
Query: 203 AGSTDVYCPYILV 165
D P +V
Sbjct: 90 PPPPDAPPPIPIV 102
>At1g26110.1 68414.m03186 expressed protein
Length = 611
Score = 29.5 bits (63), Expect = 2.6
Identities = 19/59 (32%), Positives = 30/59 (50%)
Frame = -1
Query: 392 LYLPTAGHRPSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSP 216
L +P + +P P+ PPP+ S+ S Q + L +AP SL+P S + L+P
Sbjct: 199 LPMPNSLQQPLQYPNFNTPPPPTGSS-------SLQGSSLPEAPSSLFPFSTSSQMLAP 250
>At1g15130.1 68414.m01807 hydroxyproline-rich glycoprotein family
protein
Length = 846
Score = 29.1 bits (62), Expect = 3.4
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 283 PPGWRTPHAPCTHPWPPFEDFPPH 212
PP + TPH P+PP PH
Sbjct: 785 PPPYHTPHGQAPQPYPPQAQQQPH 808
>At1g60200.1 68414.m06781 splicing factor PWI domain-containing
protein / RNA recognition motif (RRM)-containing protein
contains Pfam profiles PF01480: PWI domain, PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Length = 899
Score = 28.7 bits (61), Expect = 4.5
Identities = 18/52 (34%), Positives = 22/52 (42%)
Frame = -1
Query: 365 PSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPPP 210
P+P P+ PPP + PP A G AP + PS L PPP
Sbjct: 27 PNPNPNPSLTPPPPQQHSQPPVA-PLVPPGPPYAPPAQIPSSLLPTNLPPPP 77
>At5g64370.1 68418.m08086 beta-ureidopropionase, putative /
beta-alanine synthase, putative similar to beta-alanine
synthase [Dictyostelium discoideum] GI:14334061;
contains Pfam profile PF00795: hydrolase,
carbon-nitrogen family
Length = 408
Score = 28.3 bits (60), Expect = 5.9
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 1/43 (2%)
Frame = -1
Query: 761 GDPARSSVGCVYGVIHSQYRSDSSLPESIRY-SGALVTEIDLN 636
G P + G YG H S P RY G L++++DLN
Sbjct: 319 GKPQHNDFGHFYGSSHFSAPDASCTPSLSRYKDGLLISDMDLN 361
>At5g38560.1 68418.m04662 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 681
Score = 28.3 bits (60), Expect = 5.9
Identities = 22/63 (34%), Positives = 27/63 (42%)
Frame = -1
Query: 383 PTAGHRPSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPPPGV 204
P+A +P PS PPP S+ PP +S P P V + SPPP V
Sbjct: 32 PSAPPPVTPPPSPPQSPPPVVSSSPPPPVVSSPPPSSSPPPS---PPVIT----SPPPTV 84
Query: 203 AGS 195
A S
Sbjct: 85 ASS 87
>At5g20290.1 68418.m02415 40S ribosomal protein S8 (RPS8A) ribosomal
protein S8 - Zea mays, PIR:T04088
Length = 222
Score = 28.3 bits (60), Expect = 5.9
Identities = 19/63 (30%), Positives = 30/63 (47%)
Frame = +2
Query: 170 VCKDNIHLSSQRRQVGGKVLEWRPRMGTRSVGRPPTRWTDDLVRTAGSRWMRKAEDRIMW 349
+ +D+IH +RR GGK +WR + +GR P +T R +R + W
Sbjct: 3 ISRDSIH---KRRATGGKQKQWRKKR-KYEMGRQPANTKLSSNKTV--RRIRVRGGNVKW 56
Query: 350 KAL 358
+AL
Sbjct: 57 RAL 59
>At3g24550.1 68416.m03083 protein kinase family protein contains
Pfam domain PF00069: Protein kinase domain
Length = 652
Score = 28.3 bits (60), Expect = 5.9
Identities = 24/70 (34%), Positives = 31/70 (44%), Gaps = 7/70 (10%)
Frame = -1
Query: 383 PTAGHRPSPMP------ST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVAS-IRG 225
P+ G PSP P ST PPP+AS+ PP + S + S P +S +
Sbjct: 5 PSPGTTPSPSPPSPPTNSTTTTPPPAASS--PPPTTTPSSPPPSPSTNSTSPPPSSPLPP 62
Query: 224 LSPPPGVAGS 195
PPP GS
Sbjct: 63 SLPPPSPPGS 72
>At4g16140.1 68417.m02445 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 164
Score = 27.9 bits (59), Expect = 7.8
Identities = 19/63 (30%), Positives = 23/63 (36%)
Frame = -1
Query: 365 PSPMPST*CGPPPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPPPGVAGSTDV 186
P P PS PPPS + C S+G P YP + PPP +
Sbjct: 47 PPPPPSNPSPPPPSPTT--TACPPPPSSSG--GGPYYYYPPASQSGSYRPPPSSSSGGYY 102
Query: 185 YCP 177
Y P
Sbjct: 103 YPP 105
>At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to
SP|Q00451|PRF1_LYCES 36.4 kDa proline-rich protein
Lycopersicon esculentum, proline-rich cell wall protein
[Medicago sativa] GI:3818416; contains Pfam profile
PF00234 Protease inhibitor/seed storage/LTP family
Length = 428
Score = 27.9 bits (59), Expect = 7.8
Identities = 17/38 (44%), Positives = 19/38 (50%), Gaps = 1/38 (2%)
Frame = -2
Query: 325 PHPTTSRRAH*VVSPPG-WRTPHAPCTHPWPPFEDFPP 215
P PT VV+PP TP APC P PP +PP
Sbjct: 139 PPPTVKPPPPPVVTPPPPTPTPEAPC--PPPPPTPYPP 174
>At1g54860.1 68414.m06263 expressed protein
Length = 200
Score = 27.9 bits (59), Expect = 7.8
Identities = 13/49 (26%), Positives = 23/49 (46%)
Frame = -1
Query: 332 PPSASNDFPPCALSRQSTGLEDAPRSLYPSVASIRGLSPPPGVAGSTDV 186
PP A N+ C + LED P + + + +++ S PP + D+
Sbjct: 49 PPFAKNEKADCVADEIADKLEDQPCTNHTTASTVTPGSVPPRLTNYQDI 97
>At1g12090.1 68414.m01399 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to 14 kDa
polypeptide [Catharanthus roseus] GI:407410; contains
Pfam protease inhibitor/seed storage/LTP family domain
PF00234
Length = 137
Score = 27.9 bits (59), Expect = 7.8
Identities = 19/50 (38%), Positives = 20/50 (40%)
Frame = -1
Query: 449 AKFLLTNLLVFXXXXXXISLYLPTAGHRPSPMPST*CGPPPSASNDFPPC 300
A FL NLL F S P G PSP P P PS S+ C
Sbjct: 7 ALFLALNLLFFTTISACGSC-TPCGGGCPSPKPKPTPKPTPSPSSGSSKC 55
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,949,152
Number of Sequences: 28952
Number of extensions: 349073
Number of successful extensions: 1230
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 1012
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1203
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1712086600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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