BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0444 (748 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 34 0.025 SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Sc... 27 2.8 SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase Ade2|Sc... 26 6.6 SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding... 25 8.7 >SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr 3|||Manual Length = 512 Score = 33.9 bits (74), Expect = 0.025 Identities = 38/174 (21%), Positives = 73/174 (41%), Gaps = 5/174 (2%) Frame = +1 Query: 214 LAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKSFFKLTQ 393 L + + +LG+K GD + +A N L+ + +YA ++ +K E + + + Sbjct: 40 LQRQIASLGIKVGDPVNIAIPNGLEFVVAFYAVSWQRAICGPLNSNYKQSEFEFYIDDLK 99 Query: 394 PKIAFCQQNQ---RENYLEAARELGLDTRVITFDGDEPMSKLLXXXXXXXXXXXQPATFD 564 K+ + + AA++L + + + P S+L+ P Sbjct: 100 SKLVIVPEGSVAANTPAVRAAKKLSVAVAELAWC---PKSRLVRIVHFEGAKINAPQPLG 156 Query: 565 LDKV--YVWLISTGGTSGVLKVAAIKHKVWIRKANCLTLCLFELKDKDDTSQVI 720 L + + ++ T GT+G KV + HK R + +T + L D DTS V+ Sbjct: 157 LPQPDDVMLVLHTSGTTGRPKVVPLTHKNLCRSIHNITTS-YRL-DPRDTSYVV 208 >SPAC1F3.10c |oct1||mitochondrial intermediate peptidase Oct1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 762 Score = 27.1 bits (57), Expect = 2.8 Identities = 22/78 (28%), Positives = 37/78 (47%), Gaps = 4/78 (5%) Frame = +1 Query: 160 AATGETETNASVLQRSVR---LAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYP 330 A TG+T+ A RS R L + ++ L D + +++ IP + L N Sbjct: 466 ARTGKTDGAAHFTIRSSRELDLTSFDDSISLGFDDATNIRVKDNKRYQIPVISLLCNFVR 525 Query: 331 ITGVDPLF-KLHEIKSFF 381 +G+DP F L ++K+ F Sbjct: 526 SSGMDPTFLDLWDVKTLF 543 >SPAC144.03 |ade2|min10, min3|adenylosuccinate synthetase Ade2|Schizosaccharomyces pombe|chr 1|||Manual Length = 434 Score = 25.8 bits (54), Expect = 6.6 Identities = 21/86 (24%), Positives = 40/86 (46%), Gaps = 4/86 (4%) Frame = +1 Query: 127 RRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGR-NHLDLYIPY 303 +R +S+ + A T ++ + +++++G + G R LDL + Sbjct: 266 QRIANSIGVVKAYTTRVGAGPFPTEQLNEIGDHLQSVGREVGVTTGRKRRCGWLDLVVVK 325 Query: 304 YAALMNGYP---ITGVDPLFKLHEIK 372 Y+ ++NGY +T +D L L EIK Sbjct: 326 YSTMINGYTSLNLTKLDILDALDEIK 351 >SPCC1753.01c |ssb2|SPCC584.06c, rpa2|single-stranded DNA binding protein Ssb2|Schizosaccharomyces pombe|chr 3|||Manual Length = 279 Score = 25.4 bits (53), Expect = 8.7 Identities = 11/28 (39%), Positives = 17/28 (60%) Frame = +3 Query: 153 DRRSNRGDRDKRVSTTAVCAVSQVHADA 236 DR+ G++ + V+ + SQVHADA Sbjct: 41 DRQQGAGNKLRPVTIKQILNASQVHADA 68 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,775,124 Number of Sequences: 5004 Number of extensions: 52082 Number of successful extensions: 135 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 130 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 355273338 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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