Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0444
(748 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_2184| Best HMM Match : AMP-binding (HMM E-Value=8.5e-06) 45 8e-05
SB_36835| Best HMM Match : AMP-binding (HMM E-Value=6.2e-13) 40 0.002
SB_58684| Best HMM Match : AMP-binding (HMM E-Value=0.36) 36 0.046
SB_43771| Best HMM Match : AMP-binding (HMM E-Value=0.041) 33 0.19
SB_42391| Best HMM Match : Hydrolase (HMM E-Value=5.1e-17) 32 0.43
SB_52289| Best HMM Match : SH3_1 (HMM E-Value=3.5e-13) 29 4.0
SB_43081| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.0
>SB_2184| Best HMM Match : AMP-binding (HMM E-Value=8.5e-06)
Length = 757
Score = 44.8 bits (101), Expect = 8e-05
Identities = 49/209 (23%), Positives = 82/209 (39%)
Frame = +1
Query: 115 MDCMRRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLY 294
+DC +R D +D TGET T ++ + + G+KP DV L N +
Sbjct: 452 LDCCKRNGDKEALVDGPTGETYTYTDLITLIKKCGSALLQAGVKPKDVALLHLPNIMQYP 511
Query: 295 IPYYAALMNGYPITGVDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELGLDTRV 474
+ Y A G +T +P + + E+ + K +EAAR+ ++
Sbjct: 512 VYLYGAQAIGAVVTTANPGYTVDELAYQLIDSSAKYIITDSKLYHTAIEAARKANVEH-- 569
Query: 475 ITFDGDEPMSKLLXXXXXXXXXXXQPATFDLDKVYVWLISTGGTSGVLKVAAIKHKVWIR 654
F+ E + LL P D + + + + GT+GV K H +I
Sbjct: 570 -VFESVEFFNDLLKDDGSKLKGVYSPT--DPTEA-ICMPYSSGTTGVSKGVLQSHYNYIG 625
Query: 655 KANCLTLCLFELKDKDDTSQVIALNLSPV 741
A L F DK +++ L+L P+
Sbjct: 626 HALSLGSKEFMQWDK----RLVTLSLLPL 650
>SB_36835| Best HMM Match : AMP-binding (HMM E-Value=6.2e-13)
Length = 874
Score = 40.3 bits (90), Expect = 0.002
Identities = 21/77 (27%), Positives = 40/77 (51%)
Frame = +1
Query: 139 DSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALM 318
D +D +G++ T + ++A + G K G+VLA+ N + I Y+AA++
Sbjct: 31 DRTAIVDPQSGQSYTYPELKDLIHKMASALTRKGFKQGEVLAIMCPNIPEFAIAYFAAIL 90
Query: 319 NGYPITGVDPLFKLHEI 369
G +T ++PL+ E+
Sbjct: 91 IGGIVTSMNPLYTGREV 107
>SB_58684| Best HMM Match : AMP-binding (HMM E-Value=0.36)
Length = 237
Score = 35.5 bits (78), Expect = 0.046
Identities = 33/165 (20%), Positives = 65/165 (39%), Gaps = 3/165 (1%)
Frame = +1
Query: 154 IDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPI 333
ID+ATG++ T + + + + G + GD +A+ N ++ + Y AL G +
Sbjct: 1 IDSATGKSFTFSELCTLIRKCGSVLVRRGAQIGDTMAVILPNMIEYPVVCYGALSVGMRV 60
Query: 334 TGVDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELGLDTRVITF---DGDEPMS 504
T ++P + + E+ K +Q +AA + RV G + +
Sbjct: 61 TTLNPQYTVREMVPQLKDSQANYIITTPELIHQVNQAAAKCSCVRRVFVLADTPGHQTLY 120
Query: 505 KLLXXXXXXXXXXXQPATFDLDKVYVWLISTGGTSGVLKVAAIKH 639
+ P + D Y+ + + GT+G+ K + H
Sbjct: 121 DQILNDDGSAFPSHVPVNWKQDVAYI--LYSSGTTGLPKGVLLTH 163
>SB_43771| Best HMM Match : AMP-binding (HMM E-Value=0.041)
Length = 339
Score = 33.5 bits (73), Expect = 0.19
Identities = 26/103 (25%), Positives = 47/103 (45%)
Frame = +1
Query: 172 ETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITGVDPL 351
E T A + + + +LA + +LG+K GD + + G N + I + G + V+P
Sbjct: 75 ERATFAELREEADQLAASLLSLGIKRGDRVGIWGPNMREWVISQFGTARIGVILVNVNPA 134
Query: 352 FKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELGLDTRVIT 480
++ E + K KIA+ + R++ +D RV T
Sbjct: 135 YQAPEAEYALK----KIAYGLTETSPVTNQTRRDVPVDLRVST 173
>SB_42391| Best HMM Match : Hydrolase (HMM E-Value=5.1e-17)
Length = 254
Score = 32.3 bits (70), Expect = 0.43
Identities = 19/57 (33%), Positives = 29/57 (50%), Gaps = 1/57 (1%)
Frame = +1
Query: 340 VDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELGLDTRV-ITFDGDEPMSK 507
++PL L+ I S K KIA C + R + A LGL + + + GD+ +SK
Sbjct: 121 LEPLGDLNNIFSTLKSNNVKIAVCTADSRSGTMSALDRLGLTSMIDMVVCGDDEISK 177
>SB_52289| Best HMM Match : SH3_1 (HMM E-Value=3.5e-13)
Length = 803
Score = 29.1 bits (62), Expect = 4.0
Identities = 13/26 (50%), Positives = 16/26 (61%)
Frame = +2
Query: 335 PASIHYSNYMKSKAFLN*RNQK*RFV 412
P SIHYSN +++K FL R R V
Sbjct: 554 PESIHYSNQLQTKTFLKFRKSARRIV 579
>SB_43081| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 400
Score = 28.3 bits (60), Expect = 7.0
Identities = 13/23 (56%), Positives = 18/23 (78%), Gaps = 2/23 (8%)
Frame = -2
Query: 96 VLRV--VNVERHFIDVENTELFI 34
VLRV +V+RH ID+EN E+F+
Sbjct: 311 VLRVCRTSVKRHVIDIENNEMFM 333
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 20,519,097
Number of Sequences: 59808
Number of extensions: 375502
Number of successful extensions: 849
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 810
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 849
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 2022185256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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