BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0444 (748 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 45 5e-05 At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 43 2e-04 At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 43 2e-04 At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 41 8e-04 At1g77240.1 68414.m08996 AMP-binding protein, putative strong si... 41 0.001 At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein ... 40 0.001 At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-c... 40 0.002 At1g21540.1 68414.m02694 AMP-binding protein, putative strong si... 38 0.005 At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein ... 38 0.005 At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein ... 38 0.005 At1g21530.1 68414.m02693 AMP-binding protein, putative strong si... 36 0.038 At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase fam... 33 0.27 At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 32 0.35 At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 32 0.46 At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein ... 30 1.9 At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 29 2.5 At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase fam... 28 5.7 At1g68300.1 68414.m07802 universal stress protein (USP) family p... 28 7.6 At5g38386.1 68418.m04638 F-box family protein contains F-box dom... 27 10.0 At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 27 10.0 At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) ident... 27 10.0 >At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725 4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2) (4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana} Length = 556 Score = 45.2 bits (102), Expect = 5e-05 Identities = 34/116 (29%), Positives = 52/116 (44%), Gaps = 1/116 (0%) Frame = +1 Query: 148 CQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGY 327 C I+ TGE T A V S +LA + LG+K DV+ + N ++ + + AA G Sbjct: 54 CLINGPTGEVYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGA 113 Query: 328 PITGVDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELGLD-TRVITFDGD 492 T +P F EI K + K+ Q Y++ + L D ++T D D Sbjct: 114 ITTSANPFFTPAEISKQAKASAAKLIVTQ----SRYVDKIKNLQNDGVLIVTTDSD 165 >At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777 4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3) (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana} Length = 495 Score = 42.7 bits (96), Expect = 2e-04 Identities = 33/142 (23%), Positives = 60/142 (42%), Gaps = 1/142 (0%) Frame = +1 Query: 76 NIDDPQYHMGHLF-MDCMRRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPG 252 +ID P + H + + + D C I +TG++ T R+A + LG++ G Sbjct: 41 DIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKG 100 Query: 253 DVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKSFFKLTQPKIAFCQQNQREN 432 DV+ + +N + + A M G T +P + E+ K + K+ Sbjct: 101 DVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII----THSQ 156 Query: 433 YLEAARELGLDTRVITFDGDEP 498 Y++ + LG + +IT D P Sbjct: 157 YVDKLKNLGENLTLITTDEPTP 178 >At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777 4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3) (4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana} Length = 561 Score = 42.7 bits (96), Expect = 2e-04 Identities = 33/142 (23%), Positives = 60/142 (42%), Gaps = 1/142 (0%) Frame = +1 Query: 76 NIDDPQYHMGHLF-MDCMRRRPDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPG 252 +ID P + H + + + D C I +TG++ T R+A + LG++ G Sbjct: 41 DIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLYKLGIRKG 100 Query: 253 DVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKSFFKLTQPKIAFCQQNQREN 432 DV+ + +N + + A M G T +P + E+ K + K+ Sbjct: 101 DVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII----THSQ 156 Query: 433 YLEAARELGLDTRVITFDGDEP 498 Y++ + LG + +IT D P Sbjct: 157 YVDKLKNLGENLTLITTDEPTP 178 >At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-coumaroyl-CoA synthase, putative similar to 4CL2 [gi:12229665] from Arabidopsis thaliana, 4CL1 [gi:12229631] from Nicotiana tabacum; contains Pfam AMP-binding enzyme domain PF00501; acyl-activating enzyme superfamily; identical to cDNA 4-coumarate-CoA ligase-like protein (At4g05160) GI:29893226 Length = 544 Score = 41.1 bits (92), Expect = 8e-04 Identities = 23/89 (25%), Positives = 41/89 (46%) Frame = +1 Query: 136 PDSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAAL 315 P + D+ TG++ T + + RLA LG++ DV+ + N + + A Sbjct: 41 PSKLAIADSDTGDSLTFSQLKSAVARLAHGFHRLGIRKNDVVLIFAPNSYQFPLCFLAVT 100 Query: 316 MNGYPITGVDPLFKLHEIKSFFKLTQPKI 402 G T +PL+ ++E+ K + PKI Sbjct: 101 AIGGVFTTANPLYTVNEVSKQIKDSNPKI 129 >At1g77240.1 68414.m08996 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501 Length = 545 Score = 40.7 bits (91), Expect = 0.001 Identities = 29/115 (25%), Positives = 52/115 (45%), Gaps = 5/115 (4%) Frame = +1 Query: 166 TGETETNASVLQRSVRLAKYMRT--LGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITG 339 T T + R +R+A + + LG+ G V+++ G N +Y +A M+G + Sbjct: 36 TTTVHTWSETHSRCLRIASTLSSASLGINRGQVVSVIGPNVPSVYELQFAVPMSGAVLNN 95 Query: 340 VDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELGLDTR---VITFDGDE 495 ++P H + + ++ K+ F + LEA L D R VI DG++ Sbjct: 96 INPRLDAHALSVLLRHSESKLVFVDHHSSSLVLEAVSFLPKDERPRLVILNDGND 150 >At1g62940.1 68414.m07107 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to gi:112801 from Petroselinum crispum, GB:AAD40664 from [Solanum tuberosum] (J. Biol. Chem. 266 (13), 8551-8559 (1991)); contains Pfam AMP-binding enzyme domain PF00501 Length = 542 Score = 40.3 bits (90), Expect = 0.001 Identities = 23/78 (29%), Positives = 41/78 (52%) Frame = +1 Query: 139 DSVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALM 318 ++V ++A TG+ T V++ + RLAK + +LGL+ G V+ + N + I + Sbjct: 41 ENVAFVEAVTGKAVTYGDVVRDTKRLAKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMS 100 Query: 319 NGYPITGVDPLFKLHEIK 372 G +G +P + EIK Sbjct: 101 AGGVFSGANPTALVSEIK 118 >At3g21230.1 68416.m02683 4-coumarate--CoA ligase, putative / 4-coumaroyl-CoA synthase, putative (4CL) similar to 4CL2 [gi:12229665] and 4CL1 [gi:12229649] from [Arabidopsis thaliana], 4CL1 [gi:12229631] from Nicotiana tabacum Length = 570 Score = 39.9 bits (89), Expect = 0.002 Identities = 26/87 (29%), Positives = 39/87 (44%) Frame = +1 Query: 142 SVCQIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMN 321 + C ID ATG T A V R+A + LG++ GDV+ L N + + + A Sbjct: 62 TTCIIDGATGRILTYADVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYL 121 Query: 322 GYPITGVDPLFKLHEIKSFFKLTQPKI 402 G T +P + EI K + K+ Sbjct: 122 GAVSTTANPFYTQPEIAKQAKASAAKM 148 >At1g21540.1 68414.m02694 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 9 AMPBP9 (AMPBP9) GI:20799726 Length = 550 Score = 38.3 bits (85), Expect = 0.005 Identities = 23/95 (24%), Positives = 43/95 (45%), Gaps = 2/95 (2%) Frame = +1 Query: 166 TGETETNASVLQRSVRLAKYMRT--LGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITG 339 T T + R +R+A + + LG+ G V+++ G N +Y +A M+G + Sbjct: 37 TNTVHTWSETHNRCLRIASALTSSSLGINRGQVVSVVGPNVPSVYELQFAVPMSGAILNN 96 Query: 340 VDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEA 444 ++P H + + ++ K+ F N LEA Sbjct: 97 INPRLDAHALSVLLRHSESKLVFVDPNSISVVLEA 131 >At1g20510.2 68414.m02556 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to SP|P14912 and SP|P14913 from Petroselinum crispum; contains Pfam AMP-binding enzyme domain PF00501 Length = 473 Score = 38.3 bits (85), Expect = 0.005 Identities = 39/166 (23%), Positives = 68/166 (40%), Gaps = 3/166 (1%) Frame = +1 Query: 154 IDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPI 333 IDA+TG+ T + + +A + +G++ G V+ L N + + + + G I Sbjct: 50 IDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAII 109 Query: 334 TGVDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELG---LDTRVITFDGDEPMS 504 T +PL +EI K + P +AF AA++L +D + GD + Sbjct: 110 TTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGD--VR 167 Query: 505 KLLXXXXXXXXXXXQPATFDLDKVYVWLISTGGTSGVLKVAAIKHK 642 +L+ D D L S+ GT+G+ K H+ Sbjct: 168 RLVEMMKKEPSGNRVKERVDQDDTATLLYSS-GTTGMSKGVISSHR 212 >At1g20510.1 68414.m02555 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to SP|P14912 and SP|P14913 from Petroselinum crispum; contains Pfam AMP-binding enzyme domain PF00501 Length = 546 Score = 38.3 bits (85), Expect = 0.005 Identities = 39/166 (23%), Positives = 68/166 (40%), Gaps = 3/166 (1%) Frame = +1 Query: 154 IDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPI 333 IDA+TG+ T + + +A + +G++ G V+ L N + + + + G I Sbjct: 50 IDASTGQNLTFTELWRAVESVADCLSEIGIRKGHVVLLLSPNSILFPVVCLSVMSLGAII 109 Query: 334 TGVDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELG---LDTRVITFDGDEPMS 504 T +PL +EI K + P +AF AA++L +D + GD + Sbjct: 110 TTTNPLNTSNEIAKQIKDSNPVLAFTTSQLLPKISAAAKKLPIVLMDEERVDSVGD--VR 167 Query: 505 KLLXXXXXXXXXXXQPATFDLDKVYVWLISTGGTSGVLKVAAIKHK 642 +L+ D D L S+ GT+G+ K H+ Sbjct: 168 RLVEMMKKEPSGNRVKERVDQDDTATLLYSS-GTTGMSKGVISSHR 212 >At1g21530.1 68414.m02693 AMP-binding protein, putative strong similarity to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 10 AMPBP10 (AMPBP10) GI:20799728 Length = 547 Score = 35.5 bits (78), Expect = 0.038 Identities = 19/83 (22%), Positives = 40/83 (48%), Gaps = 2/83 (2%) Frame = +1 Query: 202 RSVRLAKYMRT--LGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKS 375 R +R+A + + +G+K G V+++ G N +Y +A M+G + ++P H + Sbjct: 49 RCLRIASALTSSSIGIKQGQVVSVVGPNVPSVYELQFAVPMSGAILNNINPRLDAHALSV 108 Query: 376 FFKLTQPKIAFCQQNQRENYLEA 444 + ++ ++ F LEA Sbjct: 109 LLRHSESRLVFVDHRSISLVLEA 131 >At1g76290.1 68414.m08860 AMP-dependent synthetase and ligase family protein similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501 Length = 546 Score = 32.7 bits (71), Expect = 0.27 Identities = 22/69 (31%), Positives = 32/69 (46%) Frame = +1 Query: 202 RSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKSFF 381 R VRLA + LGL DV+A N L Y+ A M G + ++ F + Sbjct: 48 RCVRLASALSDLGLSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMAL 107 Query: 382 KLTQPKIAF 408 + T+PK+ F Sbjct: 108 EKTKPKVFF 116 >At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to 4CL2, Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana tabacum [gi:12229631]; contains Pfam AMP-binding enzyme domain PF00501 Length = 550 Score = 32.3 bits (70), Expect = 0.35 Identities = 23/86 (26%), Positives = 41/86 (47%), Gaps = 1/86 (1%) Frame = +1 Query: 154 IDAATGETETNASVLQRSVRLAK-YMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYP 330 IDAAT + + + R+A + +G++ GDV+ + N + + I + + G Sbjct: 57 IDAATDHRISFSDLWMAVDRVADCLLHDVGIRRGDVVLVLSPNTISIPIVCLSVMSLGAV 116 Query: 331 ITGVDPLFKLHEIKSFFKLTQPKIAF 408 +T +PL EI + PK+AF Sbjct: 117 LTTANPLNTASEILRQIADSNPKLAF 142 >At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to 4CL2 [gi:12229665] from Arabidopsis thaliana, 4CL1 [gi:12229631] from Nicotiana tabacum; contains Pfam AMP-binding enzyme domain PF00501 Length = 562 Score = 31.9 bits (69), Expect = 0.46 Identities = 22/104 (21%), Positives = 49/104 (47%), Gaps = 2/104 (1%) Frame = +1 Query: 154 IDAATGETETNASVLQRSVRLAKYMRTL--GLKPGDVLALAGRNHLDLYIPYYAALMNGY 327 +++++G+ T +L+R LA +R L +V + + LD+ + Y A + G Sbjct: 70 VNSSSGDNLTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGV 129 Query: 328 PITGVDPLFKLHEIKSFFKLTQPKIAFCQQNQRENYLEAARELG 459 ++ +P+ E+ ++++P IAF + ++ LG Sbjct: 130 VVSPANPIGSESEVSHQVEVSEPVIAFATSQTVKKLQSSSLPLG 173 >At4g19010.1 68417.m02802 4-coumarate--CoA ligase family protein / 4-coumaroyl-CoA synthase family protein similar to 4CL from Pinus taeda, gi:515503, gi:1143308; contains Pfam AMP-binding enzyme domain PF00501 Length = 566 Score = 29.9 bits (64), Expect = 1.9 Identities = 21/75 (28%), Positives = 39/75 (52%), Gaps = 2/75 (2%) Frame = +1 Query: 154 IDAATGET--ETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGY 327 ID+ TG + T ++ +S+ Y LG++ GDV++L N + + + + + G Sbjct: 65 IDSLTGFSISHTELQIMVQSMAAGIY-HVLGVRQGDVVSLVLPNSVYFPMIFLSLISLGA 123 Query: 328 PITGVDPLFKLHEIK 372 +T ++P L EIK Sbjct: 124 IVTTMNPSSSLGEIK 138 >At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family protein similar to peroxisomal-coenzyme A synthetase (FAT2) [gi:586339] from Saccharomyces cerevisiae; contains Pfam AMP-binding enzyme domain PF00501; identical to cDNA; identical to cDNA adenosine monophosphate binding protein 3 AMPBP3 (AMPBP3)GI:20799714 Length = 514 Score = 29.5 bits (63), Expect = 2.5 Identities = 17/73 (23%), Positives = 32/73 (43%) Frame = +1 Query: 238 GLKPGDVLALAGRNHLDLYIPYYAALMNGYPITGVDPLFKLHEIKSFFKLTQPKIAFCQQ 417 G+KPGDV+AL N ++ I + A + ++ + E + + + K+ + Sbjct: 52 GIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAYTAEEFEFYLSDSDSKLLLTSK 111 Query: 418 NQRENYLEAAREL 456 EAA +L Sbjct: 112 EGNAPAQEAASKL 124 >At2g17650.1 68415.m02042 AMP-dependent synthetase and ligase family protein similar to AMP-binding protein GI:1903034 from [Brassica napus]; contains Pfam AMP-binding domain PF00501; identical to cDNA adenosine monophosphate binding protein 2 AMPBP2 (AMPBP2) GI:20799712 Length = 603 Score = 28.3 bits (60), Expect = 5.7 Identities = 15/42 (35%), Positives = 23/42 (54%) Frame = +1 Query: 199 QRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNG 324 QR +RLA + LG+ GDV+A N ++ ++A M G Sbjct: 94 QRCLRLASALTNLGISRGDVVAALAPNVPAMHELHFAVPMAG 135 >At1g68300.1 68414.m07802 universal stress protein (USP) family protein similar to ER6 protein [Lycopersicon esculentum] GI:5669654; contains Pfam profile PF00582: universal stress protein family Length = 160 Score = 27.9 bits (59), Expect = 7.6 Identities = 31/124 (25%), Positives = 53/124 (42%), Gaps = 11/124 (8%) Frame = +1 Query: 151 QIDAATGETETNASVLQRSVRLAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAALMNGYP 330 Q+ A E+E + LQ ++ Y++ L D++ + HLDL YA+ P Sbjct: 11 QVMVAIDESECSKRALQWTL---VYLKD-SLADSDIILFTAQPHLDLSC-VYASSYGAAP 65 Query: 331 ITGVDPLFKLHEIKSFFKLTQ-----------PKIAFCQQNQRENYLEAARELGLDTRVI 477 I ++ L + H+ +L + P+ N +E EAA +LG+D V+ Sbjct: 66 IELINSLQESHKNAGLNRLDEGTKICAETGVTPRKVLEFGNPKEAICEAAEKLGVDMLVV 125 Query: 478 TFDG 489 G Sbjct: 126 GSHG 129 >At5g38386.1 68418.m04638 F-box family protein contains F-box domain Pfam:PF00646 Length = 403 Score = 27.5 bits (58), Expect = 10.0 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = -1 Query: 730 DSELLLD-SYHLYPLIQTDTKLNNSLF*SKLCV*WQLLSELLMCH 599 D ++ D SYH+YP + + KLN +F L L EL+M H Sbjct: 116 DLSMIFDTSYHMYPQVFENKKLNFEIFLRAL----PALEELVMNH 156 >At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / acetate-CoA ligase, putative similar to SP|P27550 (Escherichia coli) and gi:8439651 (Homo sapiens); contains Pfam AMP-binding enzyme domain PF00501 Length = 693 Score = 27.5 bits (58), Expect = 10.0 Identities = 11/29 (37%), Positives = 19/29 (65%) Frame = +1 Query: 181 TNASVLQRSVRLAKYMRTLGLKPGDVLAL 267 T + +LQR +LA Y++ G+K GD + + Sbjct: 149 TYSELLQRVCQLANYLKDNGVKKGDAVVI 177 >At1g30520.1 68414.m03734 acyl-activating enzyme 14 (AAE14) identical to acyl-activating enzyme 14 [Arabidopsis thaliana]; similar to SP|Q42524 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4-coumaroyl-CoA synthase 1) {Arabidopsis thaliana}; contains Pfam profile PF00501: AMP-binding enzyme; identical to cDNA acyl-activating enzyme 14 (At1g30520) GI:29893263 Length = 560 Score = 27.5 bits (58), Expect = 10.0 Identities = 19/62 (30%), Positives = 34/62 (54%), Gaps = 1/62 (1%) Frame = +1 Query: 214 LAKYMRTLGLKPGDVLALAGRNHLDLYIPYYAAL-MNGYPITGVDPLFKLHEIKSFFKLT 390 LA + LGL+ GDV+++A N DL++ + A+ + G + ++ + L E K L Sbjct: 45 LAAGLIRLGLRNGDVVSIAAFNS-DLFLEWLLAVALVGGVVAPLNYRWSLKEAKMAMLLV 103 Query: 391 QP 396 +P Sbjct: 104 EP 105 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,316,098 Number of Sequences: 28952 Number of extensions: 264794 Number of successful extensions: 674 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 648 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 674 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1653386488 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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