BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0437 (739 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 72 7e-14 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 57 3e-09 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 56 5e-09 SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 52 9e-08 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 47 2e-06 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 42 9e-05 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 32 0.098 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.52 SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|c... 29 0.91 SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Sch... 28 1.6 SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 2.1 SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|... 26 4.9 SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosacchar... 26 6.4 SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosa... 25 8.5 SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 25 8.5 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 72.1 bits (169), Expect = 7e-14 Identities = 34/90 (37%), Positives = 51/90 (56%) Frame = +1 Query: 31 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 210 + DS + K+ + + DKLVV+DF ATWCGPCK I PK ++ + Sbjct: 5 VSDSSEFKSIVCQ--DKLVVVDFFATWCGPCKAIAPKFEQF-SNTYSDATFIKVDVDQLS 61 Query: 211 XXASEYNINSMPTFVFVKNGKKLDEFSGAN 300 A+E +++MP+F KNG+K++E GAN Sbjct: 62 EIAAEAGVHAMPSFFLYKNGEKIEEIVGAN 91 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 56.8 bits (131), Expect = 3e-09 Identities = 28/91 (30%), Positives = 47/91 (51%) Frame = +1 Query: 31 IKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXX 210 ++ D TR++ DK+ V+DF A WCGPCK + P L+++ +E Sbjct: 22 VESFGDYNTRIS--ADKVTVVDFYADWCGPCKYLKPFLEKL-SEQNQKASFIAVNADKFS 78 Query: 211 XXASEYNINSMPTFVFVKNGKKLDEFSGANV 303 A + + ++PT V + G++LD GA+V Sbjct: 79 DIAQKNGVYALPTMVLFRKGQELDRIVGADV 109 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 56.0 bits (129), Expect = 5e-09 Identities = 26/73 (35%), Positives = 38/73 (52%), Gaps = 1/73 (1%) Frame = +1 Query: 85 VVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX-ASEYNINSMPTFVFV 261 + +D A WCGPCK I P ++A++ AS + +MPTFVF Sbjct: 22 LAVDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFF 81 Query: 262 KNGKKLDEFSGAN 300 +NGK++D +GAN Sbjct: 82 ENGKQIDMLTGAN 94 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 52.0 bits (119), Expect = 9e-08 Identities = 24/74 (32%), Positives = 37/74 (50%), Gaps = 1/74 (1%) Frame = +1 Query: 76 DKLVVIDFMATWCGPCKMIGPKLDEIAAEM-XXXXXXXXXXXXXXXXXASEYNINSMPTF 252 DK++++ F A WCG CK + P+ + A E+ SEY+I PT Sbjct: 39 DKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTL 98 Query: 253 VFVKNGKKLDEFSG 294 KNGK++ ++SG Sbjct: 99 NVFKNGKQISQYSG 112 Score = 36.7 bits (81), Expect = 0.003 Identities = 19/68 (27%), Positives = 31/68 (45%) Frame = +1 Query: 79 KLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVF 258 K V+++F A WCG CK + P +++A E S +I+ PT +F Sbjct: 374 KDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDATENDIS-VSISGFPTIMF 432 Query: 259 VKNGKKLD 282 K K++ Sbjct: 433 FKANDKVN 440 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 47.2 bits (107), Expect = 2e-06 Identities = 23/94 (24%), Positives = 42/94 (44%) Frame = +1 Query: 19 MSIHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXX 198 MS+ I + + L +++++++F A W PCK + D+ A + Sbjct: 1 MSVEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKD-TKNAVFLKIEA 59 Query: 199 XXXXXXASEYNINSMPTFVFVKNGKKLDEFSGAN 300 A +++N++P FV + K L SGAN Sbjct: 60 EKFSDIAESFDVNAVPLFVLIHGAKVLARISGAN 93 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 41.9 bits (94), Expect = 9e-05 Identities = 25/94 (26%), Positives = 44/94 (46%), Gaps = 3/94 (3%) Frame = +1 Query: 25 IHIKDSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAA--EMXXXXXXXXXXX 198 + ++ ++L+ + A K +I+F ATWCG CK + P +E+ A E Sbjct: 23 VELQSLNELENTI-RASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGKIDA 81 Query: 199 XXXXXXASEYNINSMPTFV-FVKNGKKLDEFSGA 297 A +Y+I PT + F +G + ++S A Sbjct: 82 DTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNA 115 Score = 30.3 bits (65), Expect = 0.30 Identities = 10/25 (40%), Positives = 16/25 (64%) Frame = +1 Query: 79 KLVVIDFMATWCGPCKMIGPKLDEI 153 K V+++F A WCG CK + P + + Sbjct: 159 KDVLVEFYADWCGYCKRLAPTYETL 183 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 31.9 bits (69), Expect = 0.098 Identities = 22/90 (24%), Positives = 34/90 (37%), Gaps = 2/90 (2%) Frame = +1 Query: 37 DSDDLKTRLAEAGDKLVVIDFMATWCGPCKMIGPKLDEIAAEMXXXXXXXXXXXXXXXXX 216 +S + + + G LVV F A WCG CK + P ++A+ + Sbjct: 37 NSKNFRKFVKAKGPSLVV--FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQNR 94 Query: 217 A--SEYNINSMPTFVFVKNGKKLDEFSGAN 300 A S+Y + PT V K S + Sbjct: 95 AVCSQYQVQGFPTIKLVYPSSKGSSLSSTD 124 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 29.5 bits (63), Expect = 0.52 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 3/61 (4%) Frame = +1 Query: 112 CGPCKMIGPKLD---EIAAEMXXXXXXXXXXXXXXXXXASEYNINSMPTFVFVKNGKKLD 282 CG CK +GP D E A E +S NI ++PT +NG+ ++ Sbjct: 54 CGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSKELSSCANIRAVPTLYLYQNGEIVE 113 Query: 283 E 285 E Sbjct: 114 E 114 >SPAPB21F2.02 |||Dopey family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 1687 Score = 28.7 bits (61), Expect = 0.91 Identities = 11/30 (36%), Positives = 17/30 (56%) Frame = -1 Query: 457 KFITDVKNIFYSFIKNVYMFFFFNSILVSI 368 KFI D +N F+ I Y F F ++L+ + Sbjct: 436 KFILDSQNFFFESINTEYSFIIFTNLLMHL 465 >SPAC1786.03 |cut11|SPAC24C9.01|integral membrane nucleoporin|Schizosaccharomyces pombe|chr 1|||Manual Length = 601 Score = 27.9 bits (59), Expect = 1.6 Identities = 10/27 (37%), Positives = 17/27 (62%) Frame = -2 Query: 462 SSNSLQTLKTFFIHLLKTYTCFFFLIP 382 SS +L +F+ L++ + CFFF+ P Sbjct: 50 SSGPFISLSFWFLSLVRGFVCFFFMFP 76 >SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2|Schizosaccharomyces pombe|chr 1|||Manual Length = 750 Score = 27.5 bits (58), Expect = 2.1 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = -1 Query: 481 QTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGC 359 ++ + N ++TD +NIF +F+ N + F + L I C Sbjct: 71 KSSESNSIDYLTDTQNIFPNFVNNENNYQFSTAPLNPIDAC 111 >SPAC24B11.12c |||P-type ATPase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1402 Score = 26.2 bits (55), Expect = 4.9 Identities = 17/45 (37%), Positives = 22/45 (48%) Frame = -1 Query: 502 FFAFQRLQTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSI 368 +F +++T K FI KNIF F +FF F IL SI Sbjct: 84 WFPRNKIRTAKYTPIDFIP--KNIFLQFQNVANLFFLFLVILQSI 126 >SPMIT.02 |||mitochondrial DNA binding endonuclease|Schizosaccharomyces pombe|chr mitochondrial|||Manual Length = 384 Score = 25.8 bits (54), Expect = 6.4 Identities = 14/61 (22%), Positives = 28/61 (45%) Frame = -1 Query: 520 ITTEPIFFAFQRLQTQKINIFKFITDVKNIFYSFIKNVYMFFFFNSILVSIGGCVQL*SL 341 + T+ I+F + T + + + +++ F S ++N+ FF S G C+ L Sbjct: 22 VITQLIYFLTSKKITN-LGKIRLVKSIRDSFLSQLENILCFFLVYRTTYSFGVCLMKRFL 80 Query: 340 F 338 F Sbjct: 81 F 81 >SPAC167.07c ||SPAC57A7.03c|ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1029 Score = 25.4 bits (53), Expect = 8.5 Identities = 9/22 (40%), Positives = 16/22 (72%) Frame = -2 Query: 471 KLISSNSLQTLKTFFIHLLKTY 406 KL+SSN+LQ + F+ ++K + Sbjct: 412 KLVSSNTLQAMSHFYATMIKLF 433 >SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 676 Score = 25.4 bits (53), Expect = 8.5 Identities = 16/59 (27%), Positives = 30/59 (50%) Frame = -2 Query: 330 FKDSCFEFVDVSAREFVQFLAILNEDERRHRVNVVLAGDVLALINVHLHNDDGI*HFGG 154 F + +E+VD ++ + + F+ L + ++ GD + INV L + D +FGG Sbjct: 114 FSANLYEYVDGNS-DGISFVLNLENNNDTSVYHMTFHGDRVKPINVFLGSTDVTPNFGG 171 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,822,079 Number of Sequences: 5004 Number of extensions: 55649 Number of successful extensions: 198 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 185 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 193 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 349251756 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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