Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0425
(755 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1795.04c |||20S proteasome component alpha 7|Schizosaccharom... 229 2e-61
SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomy... 112 4e-26
SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha 3|Sch... 110 2e-25
SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyc... 107 2e-24
SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomy... 106 4e-24
SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyc... 94 2e-20
SPAC6G10.04c |||20S proteasome component alpha 6 subunit Pre5|Sc... 93 4e-20
SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces pom... 27 2.2
SPBC14F5.02 |||sequence orphan|Schizosaccharomyces pombe|chr 2||... 27 2.2
SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor Raf2|S... 27 3.8
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 27 3.8
SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1 |Schizo... 26 6.7
SPCC74.02c |||mRNA cleavage and polyadenylation specificity fact... 26 6.7
SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 26 6.7
SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein T... 25 8.8
>SPCC1795.04c |||20S proteasome component alpha
7|Schizosaccharomyces pombe|chr 3|||Manual
Length = 253
Score = 229 bits (561), Expect = 2e-61
Identities = 107/229 (46%), Positives = 156/229 (68%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
Q EYA KAVEN+ T IG++ +DGV+ A+EK++TSKL +P N RI +D H+G+A G I
Sbjct: 22 QAEYAYKAVENASTCIGIKCEDGVILALEKVVTSKLLKPRVNNRIGSVDRHIGIATTGFI 81
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
D + IV+ AR EA+++R YGSP+P + +R+ Y+ +T YS+VRP+G + T+
Sbjct: 82 PDGQHIVKRARDEATSWRDNYGSPIPGTVIADRLGNYVQLFTCYSSVRPFGVMSFVATY- 140
Query: 394 DYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLVH 215
D EGP +YM++P+GV + Y G A GK +Q A+ E+EKL L +K+ V+EAARI+Y H
Sbjct: 141 DSEGPHLYMVEPNGVYWGYNGAAAGKGRQVARNELEKLNFSSLKMKDAVKEAARILYATH 200
Query: 214 DELKDKQFELELSWVSKDTKGRHELVPRELATEAENQAKQALADIEDSD 68
DE +K+ E+E++WV +T G H VP EL EAE A++ +AD E+ D
Sbjct: 201 DEENNKEHEIEMTWVGVETNGIHTPVPDELLQEAEAYARR-IADGEEED 248
>SPAC323.02c |||20S proteasome component alpha 5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 247
Score = 112 bits (270), Expect = 4e-26
Identities = 70/217 (32%), Positives = 117/217 (53%), Gaps = 6/217 (2%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +A++ T IG++ KD VV VEK +TS L E + +++F ID H+G A++GL
Sbjct: 23 QVEYAIEAIKLGSTAIGVKTKDAVVLGVEKRLTSPLMESHSVEKLFEIDSHIGCAISGLT 82
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAY------TLYSAVRPYGCSV 413
+DAR I+E AR + N+R Y P ++ + + + RP+G ++
Sbjct: 83 ADARTIIEHARVQTQNHRFTYDEPQGIESTTQSICDLALRFGEGEDGEERIMSRPFGVAL 142
Query: 412 VMGTWTDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAAR 233
++ D GPQ+Y +PSG F Y A+G + AK+E+ K D+T++E +
Sbjct: 143 LI-AGIDEHGPQLYHSEPSGTYFRYEAKAIGSGSEPAKSELVKEFHKDMTLEEAEVLILK 201
Query: 232 IIYLVHDELKDKQFELELSWVSKDTKGRHELVPRELA 122
++ V +E D + ++L+ V+ + G H E+A
Sbjct: 202 VLRQVMEEKLDSK-NVQLAKVTAE-GGFHIYNDEEMA 236
>SPAC13C5.01c ||SPAC31A2.17c|20S proteasome component alpha
3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 110 bits (264), Expect = 2e-25
Identities = 59/200 (29%), Positives = 104/200 (52%), Gaps = 1/200 (0%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGL 578
QVEYA +A+ ++G +G+ KDG+V A EK +TSKL E + ++++HI +++ AVAGL
Sbjct: 20 QVEYALEAINHAGVALGIVAKDGIVLAAEKKVTSKLLEQEESAEKLYHIGDNMLCAVAGL 79
Query: 577 ISDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTW 398
+DA ++ AR Y + +P + L RV YT Y +RP+G S + W
Sbjct: 80 TADANILINYARRVGQQYLQTFNEEMPCEQLVRRVCDLKQGYTQYGGLRPFGVSFLYAGW 139
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLV 218
G Q++ +PSG S+ ++G + ++ + + D+ + E A + +
Sbjct: 140 DHIRGYQLFQSNPSGNYGSWQANSIGGNSTSVQSLMRQEYKDDINLDEASAMAVKFLSKT 199
Query: 217 HDELKDKQFELELSWVSKDT 158
D ++E + ++KDT
Sbjct: 200 LDSNSLTHEKIEFATITKDT 219
>SPBC106.16 |||20S proteasome component alpha 4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 259
Score = 107 bits (257), Expect = 2e-24
Identities = 69/223 (30%), Positives = 112/223 (50%), Gaps = 5/223 (2%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEY +AV T I LRG + +V VE+ KL ++I +D HV +A AGL
Sbjct: 19 QVEYGQEAVRRGTTAIALRGNECIVIGVERKNVPKLQNVSNFQKIAMVDNHVCLAFAGLN 78
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCS-VVMGTW 398
+DAR +++ AR EA N++ PV ++YL V+ YT VRP+G S ++ G
Sbjct: 79 ADARILIDKARVEAQNHKLNLADPVSIEYLTRYVAGVQQKYTQSGGVRPFGVSTLIAGFD 138
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLV 218
P++Y +P+G+ ++ A+G+A +AA+ +EK L+ E + A + V
Sbjct: 139 VGDNTPRVYQTEPAGIYNAWKATAIGRASKAAREYLEKNWKEGLSRDETIHLAVSSLLEV 198
Query: 217 HDELKDKQFELELSWVSKDTKGRH----ELVPRELATEAENQA 101
+ EL + KD + H + + +E+ E E +A
Sbjct: 199 -VQTASGNIELAIMDPGKDVEFLHSDKIDTIVKEIQDEKEAEA 240
>SPCC1442.06 |||20S proteasome component alpha 2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 245
Score = 106 bits (254), Expect = 4e-24
Identities = 60/173 (34%), Positives = 95/173 (54%), Gaps = 1/173 (0%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
Q+EYA AV T +G++ DGVV A EK TS+L + +++ I +GM +G+
Sbjct: 20 QIEYALNAVNAGVTSVGIKATDGVVLATEKKPTSELAIGASLEKVCAITPDIGMVYSGMG 79
Query: 574 SDARQIVETARSEA-SNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTW 398
D R +V+ +R A + Y+ Y P K L + ++ M T VRP+G S+++
Sbjct: 80 PDFRVLVDKSRKVAQTTYKKIYNEYPPTKILVQEIASVMQESTQSGGVRPFGVSLLV-AG 138
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREA 239
D +GP +Y +DPSG F++ A+GK+ AAKT +EK +L + + V A
Sbjct: 139 MDEKGPSLYQVDPSGTYFAWKATAIGKSSTAAKTFLEKRYNDELELDDAVHTA 191
>SPBC646.16 |||20S proteasome component alpha 1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 244
Score = 93.9 bits (223), Expect = 2e-20
Identities = 52/158 (32%), Positives = 82/158 (51%), Gaps = 1/158 (0%)
Frame = -2
Query: 754 QVEYAAKAVENSG-TVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGL 578
QVEYA KA N+G T +G+ GK+ +K + KL + K +F I + +G + G
Sbjct: 22 QVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGS 81
Query: 577 ISDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTW 398
I+DAR V ARSEA+ + + G P+P L +R++ T +A+RP G ++ +
Sbjct: 82 IADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMTLVAV 141
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEK 284
D GP ++ LDP+G Y + G + +EK
Sbjct: 142 DDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEK 179
>SPAC6G10.04c |||20S proteasome component alpha 6 subunit
Pre5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 93.1 bits (221), Expect = 4e-20
Identities = 64/207 (30%), Positives = 104/207 (50%), Gaps = 7/207 (3%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +A++ +GL K V K +L K++ ID+H+G+A+AGL
Sbjct: 21 QVEYALEAIKQGSATVGLVSKTHAVLVALKRNAEEL--SSYQKKLIRIDDHIGIAIAGLA 78
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCS-VVMGTW 398
DAR + + EA + ++ + P+P++ L +V+ T RPYG +V+G
Sbjct: 79 PDARVLSNYMKQEALSSKTLFTRPIPVRRLMSKVAEKAQINTQEYGRRPYGVGFLVIG-- 136
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKL--KLGDLTVKELVREAARIIY 224
D GP + PSG+ Y G ++G Q+A+T IE+ D + +EL+ A R +
Sbjct: 137 YDESGPHLLEFQPSGLVLEYLGTSMGSRSQSARTYIERNLDTFPDSSREELILSALRAL- 195
Query: 223 LVHDEL-KDKQF---ELELSWVSKDTK 155
D L KD++ + +S + KD K
Sbjct: 196 --RDTLSKDQELTEENVSISVIGKDEK 220
>SPBC6B1.10 |prp17||splicing factor Prp17|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 558
Score = 27.5 bits (58), Expect = 2.2
Identities = 18/67 (26%), Positives = 36/67 (53%), Gaps = 1/67 (1%)
Frame = -2
Query: 610 DEHVG-MAVAGLISDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAV 434
D H+G + + + ++ V T+ + + +YG+PVP+K++ + MH+ A+
Sbjct: 393 DHHLGGINSITFLENGKRFVTTSDDSSMRFW-EYGTPVPIKFV---ADIAMHSMPRV-AL 447
Query: 433 RPYGCSV 413
RP G S+
Sbjct: 448 RPNGKSI 454
>SPBC14F5.02 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 515
Score = 27.5 bits (58), Expect = 2.2
Identities = 14/51 (27%), Positives = 24/51 (47%), Gaps = 2/51 (3%)
Frame = +3
Query: 558 ICLASDMSPATAMPTCSSIWNI--LLFAPGSYSLDVINFSTANTTPSFPRR 704
I L++ + + P C +++ L + PGSY L I+ N PR+
Sbjct: 455 IALSTKLYTGSIFPKCEKDFDLHFLAYKPGSYDLSSISIEDVNHVVHKPRK 505
>SPCC970.07c |raf2|dos2, cmc2, clr7|Rik1-associated factor
Raf2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 636
Score = 26.6 bits (56), Expect = 3.8
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +2
Query: 665 LLNSKYHSVFSSEANYCSRIFYSLCSIF 748
LL +KY V+SS N C +++ ++F
Sbjct: 488 LLRNKYPKVYSSTPNLCDQVWLDRTNLF 515
>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 2310
Score = 26.6 bits (56), Expect = 3.8
Identities = 12/30 (40%), Positives = 18/30 (60%)
Frame = -3
Query: 693 KTEWYLLLRS*SHPNCMNLAQTRGYSILMN 604
+ E++LL + SHPN N A Y +L+N
Sbjct: 64 ENEYFLLRQLSSHPNGRNYAIAPAYILLLN 93
>SPBC25B2.02c |mam1|SPBC2G5.09c|M-factor transporter Mam1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1336
Score = 25.8 bits (54), Expect = 6.7
Identities = 14/44 (31%), Positives = 27/44 (61%)
Frame = -2
Query: 694 KDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLISDAR 563
K G+V +V++ I + YE + +FHI E++ ++++ LI R
Sbjct: 207 KSGLVNSVDRCI--QFYEKSISLPMFHIAENLAISLSCLIISFR 248
>SPCC74.02c |||mRNA cleavage and polyadenylation specificity factor
complex associated protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 710
Score = 25.8 bits (54), Expect = 6.7
Identities = 8/23 (34%), Positives = 16/23 (69%)
Frame = -1
Query: 326 SRKSQTGSKNRDRKAKTWRPHSE 258
S +TG+K R +K+ +W+P ++
Sbjct: 493 SNDEKTGNKKRKKKSVSWKPDND 515
>SPBC29A3.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 398
Score = 25.8 bits (54), Expect = 6.7
Identities = 16/57 (28%), Positives = 25/57 (43%), Gaps = 1/57 (1%)
Frame = -2
Query: 400 WTDYEGPQMYMLDPSGVSF-SYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAAR 233
WT + + PS F S F C V K + + + G + VKE +R+ +R
Sbjct: 317 WTSQGELPVEIFLPSSYHFHSVFTCPVSKEQATEENPPMMMSCGHVIVKESLRQLSR 373
>SPBC29A3.14c |trt1||telomerase reverse transcriptase 1 protein Trt1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 988
Score = 25.4 bits (53), Expect = 8.8
Identities = 13/32 (40%), Positives = 19/32 (59%), Gaps = 1/32 (3%)
Frame = -3
Query: 228 SILYMM-N*KISSLSWSCLGFRKTRRVAMSWF 136
S+ Y+M N KIS + W LG R ++ +S F
Sbjct: 386 SLHYLMSNIKISEIEWLVLGKRSNAKMCLSDF 417
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,958,957
Number of Sequences: 5004
Number of extensions: 59007
Number of successful extensions: 209
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 361294920
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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