Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0425
(755 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1) (... 280 8e-76
At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1) (... 135 3e-32
At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2) ... 123 1e-28
At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1) ... 122 2e-28
At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1) (... 112 2e-25
At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2) ... 112 3e-25
At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putativ... 111 4e-25
At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1) 108 3e-24
At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1) ... 106 1e-23
At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1) ... 106 2e-23
At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2) ... 104 7e-23
At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2) ... 103 1e-22
At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1) (P... 40 0.001
At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid t... 40 0.002
At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2) (P... 36 0.029
At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2) (P... 36 0.029
At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1) id... 36 0.029
At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1) id... 36 0.029
At4g16530.1 68417.m02502 expressed protein contains Pfam profile... 30 1.9
At4g32260.1 68417.m04590 ATP synthase family contains Pfam profi... 29 3.3
At2g22500.1 68415.m02669 mitochondrial substrate carrier family ... 29 3.3
At4g05110.1 68417.m00759 equilibrative nucleoside transporter, p... 29 4.4
At3g20710.1 68416.m02621 F-box protein-related contains weak hit... 28 5.8
At3g17450.1 68416.m02228 hAT dimerisation domain-containing prot... 28 7.7
At3g11590.1 68416.m01416 expressed protein 28 7.7
>At2g27020.1 68415.m03244 20S proteasome alpha subunit G (PAG1)
(PRC8) identical to proteasome subunit alpha type 3
SP:O23715, GI:12644056 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc8 GI:2511591
Length = 249
Score = 280 bits (686), Expect = 8e-76
Identities = 129/226 (57%), Positives = 173/226 (76%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
Q+EYAAKAV+NSGTV+G++ KDG+V VEKLI SK+ PG+N+RI + H GMAVAGL
Sbjct: 23 QIEYAAKAVDNSGTVVGIKCKDGIVMGVEKLIASKMMLPGSNRRIHSVHRHAGMAVAGLA 82
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
+D RQIV A+SEA +Y S YG VP+K L+ERV+ Y+H TLY +RP+GC V++G +
Sbjct: 83 ADGRQIVARAKSEARSYESVYGDAVPVKELSERVASYVHLCTLYWWLRPFGCGVILGGY- 141
Query: 394 DYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLVH 215
D +GPQ+YM++PSG+S+ YFG A+GK KQAAKTEIEKL L ++T KE V E A+IIY +H
Sbjct: 142 DRDGPQLYMIEPSGISYRYFGAAIGKGKQAAKTEIEKLNLSEMTCKEGVIEVAKIIYKLH 201
Query: 214 DELKDKQFELELSWVSKDTKGRHELVPRELATEAENQAKQALADIE 77
DE KDK FELE+SW+ +++K H+ VP +L EA+ AK AL +++
Sbjct: 202 DEAKDKAFELEMSWICEESKREHQKVPDDLLEEAKTAAKTALEEMD 247
>At3g22110.1 68416.m02791 20S proteasome alpha subunit C (PAC1)
(PRC9) identical to GB:AAC32057 from [Arabidopsis
thaliana] (Genetics (1998) 149 (2), 677-692); identical
to cDNA proteasome subunit prc9 GI:2511583
Length = 250
Score = 135 bits (327), Expect = 3e-32
Identities = 72/196 (36%), Positives = 113/196 (57%), Gaps = 1/196 (0%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGL 578
QVEYA +A+ N+G+ IG+ KDGVV EK +TSKL + + ++++ ID+HV AVAG+
Sbjct: 20 QVEYAMEAIGNAGSAIGILSKDGVVLIGEKKVTSKLLQTSTSAEKMYKIDDHVACAVAGI 79
Query: 577 ISDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTW 398
+SDA ++ TAR +A Y Y P+P++ L + + YT + +RP+G S + W
Sbjct: 80 MSDANILINTARVQAQRYTFMYQEPMPVEQLVQSLCDTKQGYTQFGGLRPFGVSFLFAGW 139
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLV 218
+ G Q+YM DPSG + AVG QAA++ +++ D T +E V A +++
Sbjct: 140 DKHHGFQLYMSDPSGNYGGWKAAAVGANNQAAQSILKQDYKDDATREEAVELALKVLTKT 199
Query: 217 HDELKDKQFELELSWV 170
D +LEL+ V
Sbjct: 200 MDSTSLTSEKLELAEV 215
>At3g14290.1 68416.m01808 20S proteasome alpha subunit E2 (PAE2)
identical to 20S proteasome subunit PAE2 GB:AAC32061
from [Arabidopsis thaliana]
Length = 237
Score = 123 bits (297), Expect = 1e-28
Identities = 68/185 (36%), Positives = 108/185 (58%), Gaps = 3/185 (1%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +A++ T IG++ K+GVV AVEK ITS L EP + ++I ID+H+G A++GLI
Sbjct: 23 QVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDDHIGCAMSGLI 82
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNER---VSMYMHAYTLYSAVRPYGCSVVMG 404
+DAR +VE AR E N+R YG P+ ++ + +++ S RP+G S+++
Sbjct: 83 ADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLI- 141
Query: 403 TWTDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIY 224
D GP +Y DPSG + A+G + A + +++ D+T++E A I+
Sbjct: 142 AGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDITLQEAETIAVSILK 201
Query: 223 LVHDE 209
V +E
Sbjct: 202 QVMEE 206
>At1g53850.1 68414.m06129 20S proteasome alpha subunit E1 (PAE1)
identical to 20S proteasome subunit PAE1 GI:3421087 from
[Arabidopsis thaliana]
Length = 237
Score = 122 bits (295), Expect = 2e-28
Identities = 68/185 (36%), Positives = 108/185 (58%), Gaps = 3/185 (1%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +A++ T IG++ K+GVV AVEK ITS L EP + ++I ID+H+G A++GLI
Sbjct: 23 QVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSVEKIMEIDDHIGCAMSGLI 82
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNER---VSMYMHAYTLYSAVRPYGCSVVMG 404
+DAR +VE AR E N+R YG P+ ++ + +++ S RP+G S+++
Sbjct: 83 ADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFGEGEEESMSRPFGVSLLI- 141
Query: 403 TWTDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIY 224
D GP +Y DPSG + A+G + A + +++ DL+++E A I+
Sbjct: 142 AGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFNKDLSLQEAETIAVSILK 201
Query: 223 LVHDE 209
V +E
Sbjct: 202 QVMEE 206
>At1g16470.1 68414.m01970 20S proteasome alpha subunit B (PAB1)
(PRC3) identical to proteasome subunit alpha type 2
SP:O23708, GI:6093778; identical to cDNA proteasome
subunit prc3 GI:2511573
Length = 235
Score = 112 bits (270), Expect = 2e-25
Identities = 57/172 (33%), Positives = 97/172 (56%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
Q+E+A AV + T +G++ +GVV A EK + S L + + ++I H+ ++G+ +G+
Sbjct: 21 QIEHALTAVGSGQTSLGIKASNGVVIATEKKLPSILVDEASVQKIQHLTPNIGVVYSGMG 80
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
D R +V +R +A Y Y P+P+ L + M +T VRP+G S+++ +
Sbjct: 81 PDFRVLVRKSRKQAEQYLRLYKEPIPVTQLVRETATVMQEFTQSGGVRPFGVSLLVAGYD 140
Query: 394 DYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREA 239
D +GPQ+Y +DPSG FS+ A+GK AKT +EK D+ + + + A
Sbjct: 141 D-KGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTEDMELDDAIHTA 191
>At5g66140.1 68418.m08332 20S proteasome alpha subunit D2 (PAD2)
(PRS1) (PRC6) identical to SP|O24616 Proteasome subunit
alpha type 7-2 (EC 3.4.25.1) (20S proteasome alpha
subunit D2) {Arabidopsis thaliana}
Length = 250
Score = 112 bits (269), Expect = 3e-25
Identities = 68/226 (30%), Positives = 121/226 (53%), Gaps = 2/226 (0%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +AV +G+RG D VV AVEK T KL + + ++I +D H+ +A AGL
Sbjct: 19 QVEYALEAVRKGNAAVGVRGTDTVVLAVEKKSTPKLQDSRSARKIVSLDNHIALACAGLK 78
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
+DAR ++ AR E ++R PV ++Y+ ++ YT VRP+G S ++ +
Sbjct: 79 ADARVLINKARIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFD 138
Query: 394 DYEG-PQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLV 218
Y P +Y DPSG ++ A G+ + + +EK + + +E ++ A R + V
Sbjct: 139 PYSRLPSLYQTDPSGTFSAWKANATGRNSNSIREFLEK-NYKESSGQETIKLAIRALLEV 197
Query: 217 HDELKDKQFELELSWVSKDTKGRHELVPREL-ATEAENQAKQALAD 83
++ +E++ ++++ G +L E+ A A+ +A++A A+
Sbjct: 198 ---VESGGKNIEVAVMTREETGLRQLEEAEIDAIVAKIEAEKAAAE 240
>At1g79210.1 68414.m09235 20S proteasome alpha subunit B, putative
nearly identical to SP|O23708 Proteasome subunit alpha
type 2 (EC 3.4.25.1) (20S proteasome alpha subunit B)
{Arabidopsis thaliana} and to At1g16470
Length = 235
Score = 111 bits (268), Expect = 4e-25
Identities = 57/172 (33%), Positives = 96/172 (55%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
Q+E+A AV + T +G++ +GVV A EK + S L + + ++I H+ ++G +G+
Sbjct: 21 QIEHALTAVGSGQTSLGIKASNGVVIATEKKLPSILVDEASVQKIQHLTPNIGTVYSGMG 80
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
D R +V +R +A Y Y P+P+ L + M +T VRP+G S+++ +
Sbjct: 81 PDFRVLVRKSRKQAEQYLRLYKEPIPVTQLVRETATVMQEFTQSGGVRPFGVSLLVAGYD 140
Query: 394 DYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREA 239
D +GPQ+Y +DPSG FS+ A+GK AKT +EK D+ + + + A
Sbjct: 141 D-KGPQLYQVDPSGSYFSWKASAMGKNVSNAKTFLEKRYTEDMELDDAIHTA 191
>At3g51260.1 68416.m05611 20S proteasome alpha subunit D (PAD1)
Length = 250
Score = 108 bits (260), Expect = 3e-24
Identities = 60/180 (33%), Positives = 94/180 (52%), Gaps = 4/180 (2%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +AV +G+RG D VV AVEK T KL + + ++I +D H+ +A AGL
Sbjct: 19 QVEYALEAVRKGNAAVGVRGTDTVVLAVEKKSTPKLQDSRSARKIVSLDNHIALACAGLK 78
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
+DAR ++ AR E ++R PV ++Y+ ++ YT VRP+G S ++ +
Sbjct: 79 ADARVLINKARIECQSHRLTLEDPVTVEYITRYIAGLQQKYTQSGGVRPFGLSTLIVGFD 138
Query: 394 DYEG-PQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIE---KLKLGDLTVKELVREAARII 227
Y P +Y DPSG ++ A G+ + + +E K G TVK +R ++
Sbjct: 139 PYTRIPALYQTDPSGTFSAWKANATGRNSNSIREFLEKNYKESAGQETVKLAIRALLEVV 198
>At5g42790.1 68418.m05212 20S proteasome alpha subunit F1 (PAF1)
(gb|AAC32062.1)
Length = 278
Score = 106 bits (255), Expect = 1e-23
Identities = 68/198 (34%), Positives = 105/198 (53%), Gaps = 2/198 (1%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +AV+ IGLR + VV A S+L ++IF +D+H+G+A+AGL
Sbjct: 21 QVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSEL--SSHQRKIFKVDDHIGVAIAGLT 78
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
+D R + RSE+ N+ Y SP+P+ L ++ T S RPYG +++G
Sbjct: 79 ADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVG-GL 137
Query: 394 DYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKL--KLGDLTVKELVREAARIIYL 221
D G +Y PSG F Y A+G QAAKT +E+ GD + ++L+++A I
Sbjct: 138 DESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFGDSSREDLIKDA---ILA 194
Query: 220 VHDELKDKQFELELSWVS 167
V + L+ + + L V+
Sbjct: 195 VRETLQGETLKSSLCTVA 212
>At5g35590.1 68418.m04237 20S proteasome alpha subunit A1 (PAA1)
(PRC1) identical to proteasome subunit alpha type 6-1
SP:O81146 GI:12643647 from [Arabidopsis thaliana];
identical to cDNA proteasome subunit prc1 GI:2511587
Length = 246
Score = 106 bits (254), Expect = 2e-23
Identities = 68/214 (31%), Positives = 113/214 (52%), Gaps = 4/214 (1%)
Frame = -2
Query: 754 QVEYAAKAVENSG-TVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGL 578
QVEYA KAV+ +G T IG+RGKD V +K + KL + + +F I +++G+ G+
Sbjct: 24 QVEYAFKAVKTAGITSIGVRGKDSVCVVTQKKVPDKLLDQSSVTHLFPITKYIGLVATGI 83
Query: 577 ISDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYG-CSVVMGT 401
+DAR +V+ AR++A+ +R YG +P+ L + ++ YT ++ +RP G ++VMG
Sbjct: 84 TADARSLVQQARNQAAEFRFTYGYEMPVDILAKWIADKSQVYTQHAYMRPLGVVAMVMGV 143
Query: 400 WTDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIE-KLKLG-DLTVKELVREAARII 227
+ GP +Y DP+G + + + G +Q A +E K+K T E V+ A +
Sbjct: 144 -DEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKENPSFTFDETVQTAISAL 202
Query: 226 YLVHDELKDKQFELELSWVSKDTKGRHELVPREL 125
V E K E+E+ V + L E+
Sbjct: 203 QSVLQE-DFKATEIEVGVVRAENPEFRALTTEEI 235
>At2g05840.1 68415.m00632 20S proteasome alpha subunit A2 (PAA2)
identical to GB:AF043519
Length = 246
Score = 104 bits (249), Expect = 7e-23
Identities = 64/202 (31%), Positives = 108/202 (53%), Gaps = 4/202 (1%)
Frame = -2
Query: 754 QVEYAAKAVENSG-TVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGL 578
QVEYA KAV+ +G T IG+RGKD V +K + KL + + +F + +++G+ G+
Sbjct: 24 QVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKLLDQSSVSHLFPVTKYLGLLATGM 83
Query: 577 ISDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTW 398
+D+R +V+ AR+EA+ +R QYG +P L + ++ YT ++ +RP G ++
Sbjct: 84 TADSRSLVQQARNEAAEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAMVLGI 143
Query: 397 TDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGD---LTVKELVREAARII 227
+ GP +Y DP+G + + + G +Q A +EK K+ + T E V+ A +
Sbjct: 144 DEERGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEK-KMKENPAFTYDETVQTAISAL 202
Query: 226 YLVHDELKDKQFELELSWVSKD 161
V E K E+E+ V D
Sbjct: 203 QSVLQE-DFKATEIEVGVVRAD 223
>At1g47250.1 68414.m05231 20S proteasome alpha subunit F2 (PAF2)
(PRC2B) (PRS1) identical to GB:AAC32063 from
[Arabidopsis thaliana] (Genetics 149 (2), 677-692
(1998)); identical to cDNA proteasome subunit prc2b
GI:2511585
Length = 277
Score = 103 bits (248), Expect = 1e-22
Identities = 72/235 (30%), Positives = 118/235 (50%), Gaps = 3/235 (1%)
Frame = -2
Query: 754 QVEYAAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGANKRIFHIDEHVGMAVAGLI 575
QVEYA +AV+ IGLR + VV A S+L ++IF +D+H+G+A+AGL
Sbjct: 21 QVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSEL--SSHQRKIFKVDDHIGVAIAGLT 78
Query: 574 SDARQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWT 395
+D R + RSE+ N+ Y SP+P+ L ++ T S RPYG +++G
Sbjct: 79 ADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCTQRSWKRPYGVGLLVG-GL 137
Query: 394 DYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKL--KLGDLTVKELVREAARIIYL 221
D G +Y PSG F Y A+G QAAKT +E+ + + ++L+++A I
Sbjct: 138 DESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERKFESFQESSKEDLIKDA---IMA 194
Query: 220 VHDELKDKQFELELSWVS-KDTKGRHELVPRELATEAENQAKQALADIEDSDEGE 59
+ + L+ + + L VS + +E + + ++ + ED+ EGE
Sbjct: 195 IRETLQGETLKSSLCTVSVLGVDEPFHFLDQESIQKVIDTFEKVPEEEEDAGEGE 249
>At4g31300.1 68417.m04441 20S proteasome beta subunit A (PBA1)
(PRCD) identical to cDNA proteasome subunit prcd
GI:2511593
Length = 233
Score = 40.3 bits (90), Expect = 0.001
Identities = 27/122 (22%), Positives = 56/122 (45%), Gaps = 1/122 (0%)
Frame = -2
Query: 715 TVIGLRGKDGVVFAVEKLITSKLYEPG-ANKRIFHIDEHVGMAVAGLISDARQIVETARS 539
T+IG+ GVV + ++ +Y A+ +I + ++V + +G +D++ + + R
Sbjct: 14 TIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYVRY 73
Query: 538 EASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYEGPQMYMLDP 359
+ Q+G P +K VS + Y+ +++G W YEG ++Y +
Sbjct: 74 FLHQHTIQHGQPATVK-----VSANLIRMLAYNNKNMLQTGLIVGGWDKYEGGKIYGIPL 128
Query: 358 SG 353
G
Sbjct: 129 GG 130
>At4g15160.1 68417.m02327 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein similar to
SP|Q00451|PRF1_LYCES 36.4 kDa proline-rich protein
Lycopersicon esculentum, proline-rich cell wall protein
[Medicago sativa] GI:3818416; contains Pfam profile
PF00234 Protease inhibitor/seed storage/LTP family
Length = 428
Score = 39.9 bits (89), Expect = 0.002
Identities = 19/39 (48%), Positives = 28/39 (71%), Gaps = 1/39 (2%)
Frame = -2
Query: 694 KDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAG 581
KDGVV EK +TSKL + ++ ++++ ID+HV AVAG
Sbjct: 322 KDGVVLVGEKKVTSKLLQTSSSMEKMYKIDDHVACAVAG 360
Score = 37.5 bits (83), Expect = 0.010
Identities = 20/58 (34%), Positives = 33/58 (56%)
Frame = -2
Query: 400 WTDYEGPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARII 227
W G Q+YM DPSG + AVG QAA++ +++ D T +E+V+ A +++
Sbjct: 361 WDRNHGFQLYMSDPSGNYGGWQAAAVGANNQAAQSILKQDYKDDATREEVVQLAIKVL 418
>At5g40580.2 68418.m04925 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 35.9 bits (79), Expect = 0.029
Identities = 36/173 (20%), Positives = 70/173 (40%), Gaps = 1/173 (0%)
Frame = -2
Query: 742 AAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGLISDA 566
A ++ T++GL KDGV+ + T N ++I ++ ++ AG +D
Sbjct: 32 APSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
Query: 565 RQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYE 386
+ + S+ +R Q G + + + H ++ V ++V+G D
Sbjct: 92 EAVTDMVSSQLRLHRYQTGR--DSRVVTALTLLKKHLFSYQGHV---SAALVLG-GVDIT 145
Query: 385 GPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARII 227
GP ++ + P G + + +G AA + E LT E ++ A I
Sbjct: 146 GPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAI 198
>At5g40580.1 68418.m04924 20S proteasome beta subunit B (PBB2)
(PRCFC) identical to 20S proteasome beta subunit PBB2
[Arabidopsis thaliana] GI:3421104, cDNA proteasome
subunit prcfc GI:2511575
Length = 274
Score = 35.9 bits (79), Expect = 0.029
Identities = 36/173 (20%), Positives = 70/173 (40%), Gaps = 1/173 (0%)
Frame = -2
Query: 742 AAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGLISDA 566
A ++ T++GL KDGV+ + T N ++I ++ ++ AG +D
Sbjct: 32 APSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
Query: 565 RQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYE 386
+ + S+ +R Q G + + + H ++ V ++V+G D
Sbjct: 92 EAVTDMVSSQLRLHRYQTGR--DSRVVTALTLLKKHLFSYQGHV---SAALVLG-GVDIT 145
Query: 385 GPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARII 227
GP ++ + P G + + +G AA + E LT E ++ A I
Sbjct: 146 GPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAI 198
>At3g27430.2 68416.m03429 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 273
Score = 35.9 bits (79), Expect = 0.029
Identities = 36/173 (20%), Positives = 70/173 (40%), Gaps = 1/173 (0%)
Frame = -2
Query: 742 AAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGLISDA 566
A ++ T++GL KDGV+ + T N ++I ++ ++ AG +D
Sbjct: 32 APSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
Query: 565 RQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYE 386
+ + S+ +R Q G + + + H ++ V ++V+G D
Sbjct: 92 EAVTDMVSSQLRLHRYQTGR--DSRVITALTLLKKHLFSYQGHV---SAALVLG-GVDIT 145
Query: 385 GPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARII 227
GP ++ + P G + + +G AA + E LT E ++ A I
Sbjct: 146 GPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAESI 198
>At3g27430.1 68416.m03428 20S proteasome beta subunit B (PBB1)
identical to 20S proteasome beta subunit PBB1 (PBB1)
GB:AAC32066 [Arabidopsis thaliana] (Genetics 149 (2),
677-692 (1998)); contains Pfam profile: PF00227
proteasome A-type and B-type;
Length = 267
Score = 35.9 bits (79), Expect = 0.029
Identities = 36/173 (20%), Positives = 70/173 (40%), Gaps = 1/173 (0%)
Frame = -2
Query: 742 AAKAVENSGTVIGLRGKDGVVFAVEKLITSKLYEPGAN-KRIFHIDEHVGMAVAGLISDA 566
A ++ T++GL KDGV+ + T N ++I ++ ++ AG +D
Sbjct: 32 APSFLKTGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAADT 91
Query: 565 RQIVETARSEASNYRSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYE 386
+ + S+ +R Q G + + + H ++ V ++V+G D
Sbjct: 92 EAVTDMVSSQLRLHRYQTGR--DSRVITALTLLKKHLFSYQGHV---SAALVLG-GVDIT 145
Query: 385 GPQMYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARII 227
GP ++ + P G + + +G AA + E LT E ++ A I
Sbjct: 146 GPHLHTIYPHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAESI 198
>At4g16530.1 68417.m02502 expressed protein contains Pfam profile
PF04510: Family of unknown function (DUF577)
Length = 774
Score = 29.9 bits (64), Expect = 1.9
Identities = 22/73 (30%), Positives = 43/73 (58%), Gaps = 2/73 (2%)
Frame = -2
Query: 307 AAKTEIEKLKLGDLTVKELVREAARIIYLVHDELKDKQFELELSW--VSKDTKGRHELVP 134
A+ EIE + +G T KE+VR+ ++ +D+LK+++ E E W + KD E++
Sbjct: 284 ASMNEIEGV-VGTET-KEIVRKIKMLVTEPYDDLKNRREEFERGWYDILKDLSSL-EVLK 340
Query: 133 RELATEAENQAKQ 95
++E E+++K+
Sbjct: 341 ILASSELEDRSKE 353
>At4g32260.1 68417.m04590 ATP synthase family contains Pfam profile:
PF00430 ATP synthase B/B' CF(0); identical to cDNA
chloroplast ATP synthase beta chain precursor (atpG)
GI:5730140
Length = 219
Score = 29.1 bits (62), Expect = 3.3
Identities = 27/104 (25%), Positives = 47/104 (45%)
Frame = -2
Query: 376 MYMLDPSGVSFSYFGCAVGKAKQAAKTEIEKLKLGDLTVKELVREAARIIYLVHDELKDK 197
M+ LD V +S G + + + K ++ +K VKEL +AA ++ E+
Sbjct: 99 MFALDK--VYYSPLGNFMDQRDASIKEKLASVKDTSTEVKELDEQAAAVMRAARAEIA-- 154
Query: 196 QFELELSWVSKDTKGRHELVPRELATEAENQAKQALADIEDSDE 65
L+ + K+T+ E E + E + K+ALA +E E
Sbjct: 155 ---AALNKMKKETQVEVEEKLAEGRKKVEEELKEALASLESQKE 195
>At2g22500.1 68415.m02669 mitochondrial substrate carrier family
protein contains Pfam profile: PF00153 mitochondrial
carrier protein
Length = 313
Score = 29.1 bits (62), Expect = 3.3
Identities = 20/72 (27%), Positives = 30/72 (41%), Gaps = 2/72 (2%)
Frame = -2
Query: 496 LKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYEGPQMYMLDP--SGVSFSYFGCAV 323
++ L VS + TLYS R ++ G WTD E M ++ +G G AV
Sbjct: 77 MRALFSGVSATVLRQTLYSTTRMGLYDIIKGEWTDPETKTMPLMKKIGAGAIAGAIGAAV 136
Query: 322 GKAKQAAKTEIE 287
G A ++
Sbjct: 137 GNPADVAMVRMQ 148
>At4g05110.1 68417.m00759 equilibrative nucleoside transporter,
putative (ENT6) identical to putative equilibrative
nucleoside transporter ENT6 [Arabidopsis thaliana]
GI:16518997; contains similarity to SWISS-PROT:O54699
equilibrative nucleoside transporter 2 (Equilibrative
nitrobenzylmercaptopurine riboside-insensitive
nucleoside transporter, Equilibrative NBMPR-insensitive
nucleoside transporter, Nucleoside transporter, ei-type)
[Rattus norvegicus]; contains Pfam profile PF01733:
Nucleoside transporter
Length = 418
Score = 28.7 bits (61), Expect = 4.4
Identities = 18/53 (33%), Positives = 30/53 (56%), Gaps = 2/53 (3%)
Frame = -2
Query: 598 GMAVAGLISDARQIVETARSEASNYRSQYGSPVPLKYLN--ERVSMYMHAYTL 446
GMAVAG ++ A +++ A E SN + G+ + L E +S+ ++AY L
Sbjct: 154 GMAVAGALTSALRLITKAAFEKSNNGLRKGAMMFLAISTCIELLSVMLYAYVL 206
>At3g20710.1 68416.m02621 F-box protein-related contains weak hit to
TIGRFAM TIGR01640 : F-box protein interaction domain;
contains weak hit to Pfam PF00646: F-box domain
Length = 362
Score = 28.3 bits (60), Expect = 5.8
Identities = 17/63 (26%), Positives = 33/63 (52%)
Frame = -2
Query: 523 RSQYGSPVPLKYLNERVSMYMHAYTLYSAVRPYGCSVVMGTWTDYEGPQMYMLDPSGVSF 344
R ++G+P+PL + NE +++ Y SA++ +V++ W ++P VS+
Sbjct: 225 RERFGTPLPLPFSNEGYTLH-KGYKSLSALKEEKLAVLL--WDTMVIWVTNKIEPDAVSW 281
Query: 343 SYF 335
S F
Sbjct: 282 SIF 284
>At3g17450.1 68416.m02228 hAT dimerisation domain-containing protein
contains Pfam profile PF04937: Protein of unknown
function (DUF 659)
Length = 877
Score = 27.9 bits (59), Expect = 7.7
Identities = 13/30 (43%), Positives = 20/30 (66%), Gaps = 3/30 (10%)
Frame = -2
Query: 424 GCSVVMGTWTDYEGPQM--YMLD-PSGVSF 344
GCS++ TWT+ EG +M +++ P GV F
Sbjct: 374 GCSIMADTWTNTEGKKMISFLVSCPRGVYF 403
>At3g11590.1 68416.m01416 expressed protein
Length = 622
Score = 27.9 bits (59), Expect = 7.7
Identities = 14/42 (33%), Positives = 26/42 (61%)
Frame = -2
Query: 301 KTEIEKLKLGDLTVKELVREAARIIYLVHDELKDKQFELELS 176
K E+E+LK VKE V + ++ L D L++++ +++LS
Sbjct: 378 KAEVEELKRESFKVKEEVEKEREMLQLA-DALREERVQMKLS 418
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,413,677
Number of Sequences: 28952
Number of extensions: 309048
Number of successful extensions: 1108
Number of sequences better than 10.0: 25
Number of HSP's better than 10.0 without gapping: 1058
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1091
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1682736544
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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