BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0422 (447 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 31 0.081 SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pomb... 24 9.3 >SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1935 Score = 31.1 bits (67), Expect = 0.081 Identities = 22/72 (30%), Positives = 36/72 (50%), Gaps = 1/72 (1%) Frame = +1 Query: 25 PLLDYKKNNTINIYAGRIIDVNLVENRKFLDSLPPTKLFN*ISNPYL*IESFVSIFT-LH 201 PLL K+ +++ AGR+ D ++ K L P L SN Y+ ++ ++ L Sbjct: 753 PLLGSKRRELVSVAAGRLADNQMIVYNKKNGYLIPKDLGRIASNYYINYQTVSTLNNLLK 812 Query: 202 SKTTEQILIRLL 237 SK +E +I LL Sbjct: 813 SKMSEADIIALL 824 >SPBC11G11.07 ||SPBC18H10.01|karyopherin|Schizosaccharomyces pombe|chr 2|||Manual Length = 955 Score = 24.2 bits (50), Expect = 9.3 Identities = 12/48 (25%), Positives = 23/48 (47%) Frame = +2 Query: 116 IVCHLQNSLIK*VILTYK*KVL*VFSLFIQKLLNRF**DFYAGLLWLA 259 ++C + L+K I T+K K + + + L+ F Y LW++ Sbjct: 668 LICERISKLLKNFIYTFKEKAIVTLPVITEALIKGFEKTQYGCFLWVS 715 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,620,959 Number of Sequences: 5004 Number of extensions: 31586 Number of successful extensions: 57 Number of sequences better than 10.0: 2 Number of HSP's better than 10.0 without gapping: 57 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 57 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 164204010 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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