SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0414
         (793 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein...    74   2e-14
SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual      62   1e-10
SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr ...    36   0.005
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po...    27   2.3  
SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase Ubp4|Schizos...    26   7.1  
SPBC1826.01c |mot1||TATA-binding protein associated factor Mot1|...    26   7.1  
SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase Su...    26   7.1  
SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein Sad1|S...    26   7.1  
SPBC83.05 |||mitochondrial RNA-binding protein |Schizosaccharomy...    25   9.4  
SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr 1|...    25   9.4  

>SPAC4F8.10c |stg1||SM22/transgelin-like actin modulating protein
           Stg1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 174

 Score = 74.1 bits (174), Expect = 2e-14
 Identities = 48/122 (39%), Positives = 67/122 (54%), Gaps = 2/122 (1%)
 Frame = -1

Query: 682 DVLRDGVILCKLANKLAPGSVKKIQEKGTNFQLMENIQRF-QAAIKKYGVPEEEIFQTAD 506
           D L+ GVILC++  K A G+  + +E    F  MENI  F   A +   VP +++FQT+D
Sbjct: 29  DQLQSGVILCRIC-KEALGANIRYKESNMPFVQMENISAFINYAQQVVHVPSQDMFQTSD 87

Query: 505 LFERRNIPQVTLCLYALGRITQKHPEFTGPQLGPKMADKNERTFTEEQLRAHNAELN-LQ 329
           LFERRN  QV   +++  R   K        LGPK+A+K  R F+ +Q R     +N LQ
Sbjct: 88  LFERRNDEQVLRSIHSFSRYAAKMFPGKVRGLGPKLAEKKPRVFSAQQQREFREGVNSLQ 147

Query: 328 MG 323
            G
Sbjct: 148 YG 149


>SPAC4F8.13c |rng2||IQGAP|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1489

 Score = 61.7 bits (143), Expect = 1e-10
 Identities = 33/90 (36%), Positives = 49/90 (54%), Gaps = 2/90 (2%)
 Frame = -1

Query: 718 CLGEPL-PNGAYEDVLRDGVILCKLANKLAPGSVKKI-QEKGTNFQLMENIQRFQAAIKK 545
           CLG  L P   +E  LR+GV+L  L  K  P  + KI       F+  +NI +F   I  
Sbjct: 54  CLGTDLGPTSTFEQSLRNGVVLALLVQKFQPDKLIKIFYSNELQFRHSDNINKFLDFIHG 113

Query: 544 YGVPEEEIFQTADLFERRNIPQVTLCLYAL 455
            G+PE   F+  D++E +N+P+V  C++AL
Sbjct: 114 IGLPEIFHFELTDIYEGKNLPKVIYCIHAL 143


>SPBC17D1.07c |||GTPase regulator |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 962

 Score = 36.3 bits (80), Expect = 0.005
 Identities = 27/82 (32%), Positives = 39/82 (47%), Gaps = 4/82 (4%)
 Frame = -1

Query: 688 YEDVLRDGVILCKLANKLAPGSVKKIQEKGTNFQLMEN----IQRFQAAIKKYGVPEEEI 521
           + D L +G +LC+LA K  P   K        +Q+ E     +  F   +   G+     
Sbjct: 88  FVDALVNGKVLCQLAFKYYP---KLASNWKPRYQISERNTVYLNAFFHFLDFIGMFTPFR 144

Query: 520 FQTADLFERRNIPQVTLCLYAL 455
           F+T DL  R NIP+V  CL+AL
Sbjct: 145 FETKDLVRRFNIPKVIYCLHAL 166


>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 401

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 10/17 (58%), Positives = 12/17 (70%)
 Frame = +1

Query: 631 EPICWPICKGSHRPLAH 681
           +PIC+P  K SH PL H
Sbjct: 360 DPICFPWKKASHMPLEH 376


>SPBC18H10.08c |ubp4||ubiquitin C-terminal hydrolase
           Ubp4|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 438

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 14/41 (34%), Positives = 21/41 (51%)
 Frame = +3

Query: 579 SMSWKLVPFSWIFFTDPGANLLANLQRITPSLSTSSYAPFG 701
           S+++KL         DP    LAN +++  SLS  +Y P G
Sbjct: 33  SLAYKLYSMLRSLKLDPHFMELANPKKVDSSLSYENYQPIG 73


>SPBC1826.01c |mot1||TATA-binding protein associated factor
           Mot1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1953

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = +2

Query: 716 TSEIQVRTSCSWSLFLAGILCRRNLV 793
           T  I V+TSCSW   L   LC +N++
Sbjct: 638 TKFISVQTSCSWITGLTLRLCFQNVL 663


>SPCC1795.11 |sum3|ded1, slh3, moc2|ATP-dependent RNA helicase
           Sum3|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 636

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 14/52 (26%), Positives = 21/52 (40%)
 Frame = -1

Query: 439 KHPEFTGPQLGPKMADKNERTFTEEQLRAHNAELNLQMGFNKGASQSGHGGF 284
           +   F G   G + + +  R       R      N   G++ G S SG+GGF
Sbjct: 565 RESSFGGNGRGGRYSGRGGRGGNAYGARDFRRPTNSSSGYSSGPSYSGYGGF 616


>SPBC12D12.01 |sad1|SPBC16H5.01c|spindle pole body protein
           Sad1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 514

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = -2

Query: 330 KWASIRAPPSPVMVASVTPATCKLLLNFSQYF 235
           ++  IR+PP+ V + S+TP       NF Q +
Sbjct: 46  RYTKIRSPPTRVSIESITPKQRFPAPNFEQAY 77


>SPBC83.05 |||mitochondrial RNA-binding protein |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 773

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 11/21 (52%), Positives = 12/21 (57%)
 Frame = -1

Query: 238 FSTLISVFVHGRIWLNDFFTF 176
           FST I   VHG  WL D + F
Sbjct: 140 FSTPIPHMVHGIPWLQDLYIF 160


>SPAC637.09 |||ribonuclease H70 |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 623

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 8/18 (44%), Positives = 11/18 (61%)
 Frame = +3

Query: 468 HNVTWGMLRLSKRSAVWN 521
           HN  WG  R  + +A+WN
Sbjct: 491 HNFVWGRFRELEHAAMWN 508


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,530,718
Number of Sequences: 5004
Number of extensions: 80962
Number of successful extensions: 220
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 219
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -