Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0414
(793 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g47500.1 68415.m05929 kinesin motor protein-related 50 2e-06
At5g27000.1 68418.m03221 kinesin motor protein-related non-conse... 48 7e-06
At1g09170.1 68414.m01024 kinesin motor protein-related similar t... 40 0.003
At1g63640.2 68414.m07198 kinesin motor protein-related C-termina... 38 0.008
At1g63640.1 68414.m07197 kinesin motor protein-related C-termina... 38 0.008
At3g10310.1 68416.m01237 kinesin motor protein-related similar t... 38 0.010
At5g41310.1 68418.m05020 kinesin motor protein-related 37 0.013
At3g44730.1 68416.m04814 kinesin motor protein-related similar t... 36 0.041
At5g35700.1 68418.m04269 fimbrin-like protein, putative similar ... 34 0.094
At5g48460.1 68418.m05992 fimbrin-like protein, putative strong s... 33 0.22
At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical t... 33 0.22
At5g55400.1 68418.m06902 fimbrin-like protein, putative similar ... 33 0.29
At2g04750.1 68415.m00485 fimbrin-like protein, putative similar ... 32 0.38
At5g62240.1 68418.m07815 expressed protein various predicted pro... 31 0.66
At5g10050.1 68418.m01164 short-chain dehydrogenase/reductase (SD... 29 2.7
At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloropl... 29 2.7
At2g25730.1 68415.m03084 expressed protein 29 3.5
At5g25475.2 68418.m03031 expressed protein 28 8.2
At5g25475.1 68418.m03030 expressed protein 28 8.2
At2g46510.1 68415.m05796 basic helix-loop-helix (bHLH) family pr... 28 8.2
>At2g47500.1 68415.m05929 kinesin motor protein-related
Length = 974
Score = 50.0 bits (114), Expect = 2e-06
Identities = 30/86 (34%), Positives = 46/86 (53%), Gaps = 12/86 (13%)
Frame = -1
Query: 676 LRDGVILCKLANKLAPGSVKKIQEKGTN------------FQLMENIQRFQAAIKKYGVP 533
LR G+ILCK+ NK+ PG+V K+ E + FQ EN++ F AI++ G P
Sbjct: 79 LRSGIILCKVLNKVQPGAVSKVVESPCDAILVADGAPLSAFQYFENVRNFLVAIQEMGFP 138
Query: 532 EEEIFQTADLFERRNIPQVTLCLYAL 455
F+ +DL + N +V C+ A+
Sbjct: 139 ---TFEASDLEQGGNASRVVNCVLAI 161
>At5g27000.1 68418.m03221 kinesin motor protein-related
non-consensus AT donor splice site at exon 12;
non-consensus AC acceptor splice site at exon 13
Length = 987
Score = 48.0 bits (109), Expect = 7e-06
Identities = 28/83 (33%), Positives = 45/83 (54%), Gaps = 9/83 (10%)
Frame = -1
Query: 676 LRDGVILCKLANKLAPGSVKKIQEKGTN---------FQLMENIQRFQAAIKKYGVPEEE 524
LR G++LC + NK+ PGSV K+ E + FQ ENI+ F AI++ G+P
Sbjct: 79 LRSGIVLCNVLNKVNPGSVSKVVEAPDDVADGAALSAFQYFENIRNFLVAIEEMGLPS-- 136
Query: 523 IFQTADLFERRNIPQVTLCLYAL 455
F+ +D+ + ++ C+ AL
Sbjct: 137 -FEASDMEKGGKSIRIVNCILAL 158
>At1g09170.1 68414.m01024 kinesin motor protein-related similar to
GB:AAB61066
Length = 1010
Score = 39.5 bits (88), Expect = 0.003
Identities = 23/86 (26%), Positives = 43/86 (50%), Gaps = 12/86 (13%)
Frame = -1
Query: 676 LRDGVILCKLANKLAPGSVKKIQEKGTN------------FQLMENIQRFQAAIKKYGVP 533
LR G++LC + N++ PG+V K+ E + FQ EN++ F +++ G+P
Sbjct: 89 LRSGILLCNVLNRVKPGAVPKVVEAPNDPLVNQDGAALSAFQYFENLRNFLVFVEEMGIP 148
Query: 532 EEEIFQTADLFERRNIPQVTLCLYAL 455
F+ +D + ++ C+ AL
Sbjct: 149 ---TFEVSDFEKGGKSARIVECVLAL 171
>At1g63640.2 68414.m07198 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1065
Score = 37.9 bits (84), Expect = 0.008
Identities = 20/53 (37%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = -1
Query: 676 LRDGVILCKLANKLAPGSVKKIQEKGTNFQ-LMENIQRFQAAIKKYGVPEEEI 521
LRDG +LC L N+L+PGS++ G +F+ I+RF A+ + +P E+
Sbjct: 71 LRDGTVLCSLLNQLSPGSMR----MGGSFEPASVKIERFLTAMDEMALPRFEV 119
>At1g63640.1 68414.m07197 kinesin motor protein-related C-terminal
region is similar to C-term region of kinesin motor
protein GB:AAB51397 (Mus musculus); contains Pfam
profile: PF00225 Kinesin motor domain
Length = 1064
Score = 37.9 bits (84), Expect = 0.008
Identities = 20/53 (37%), Positives = 32/53 (60%), Gaps = 1/53 (1%)
Frame = -1
Query: 676 LRDGVILCKLANKLAPGSVKKIQEKGTNFQ-LMENIQRFQAAIKKYGVPEEEI 521
LRDG +LC L N+L+PGS++ G +F+ I+RF A+ + +P E+
Sbjct: 71 LRDGTVLCSLLNQLSPGSMR----MGGSFEPASVKIERFLTAMDEMALPRFEV 119
>At3g10310.1 68416.m01237 kinesin motor protein-related similar to
carboxy-terminal kinesin 2 GB:P79955 [Xenopus laevis]
Length = 897
Score = 37.5 bits (83), Expect = 0.010
Identities = 25/85 (29%), Positives = 41/85 (48%), Gaps = 11/85 (12%)
Frame = -1
Query: 676 LRDGVILCKLANKLAPGSVKKIQEKGT----------NFQLMENIQRFQAAIKKYGVPEE 527
LR+G+ILC NK+ PG+V K+ E + +Q EN++ F A++ +P
Sbjct: 51 LRNGMILCNAINKIHPGAVSKVVENYSYLNGEYQLPPAYQYFENVRNFLVALETLRLPGF 110
Query: 526 EIFQ-TADLFERRNIPQVTLCLYAL 455
E D E ++ +V C+ L
Sbjct: 111 EASDLEKDNLESGSVTKVVDCILGL 135
>At5g41310.1 68418.m05020 kinesin motor protein-related
Length = 961
Score = 37.1 bits (82), Expect = 0.013
Identities = 26/65 (40%), Positives = 38/65 (58%), Gaps = 5/65 (7%)
Frame = -1
Query: 703 LPNGAYEDVLR----DGVILCKLANKLAPGSVKKIQEKGTNFQL-MENIQRFQAAIKKYG 539
LP A E+ LR DG +LC L N+L+PGS++ G +F+ NI+RF AA+ +
Sbjct: 59 LPWEASEEELRACLVDGTVLCNLLNQLSPGSMR----MGGSFEPGCVNIERFLAAMDEMT 114
Query: 538 VPEEE 524
+P E
Sbjct: 115 LPRFE 119
>At3g44730.1 68416.m04814 kinesin motor protein-related similar to 4
other kinesin-like proteins of A. thaliana: F02P16.12
(PID:g2191180), katA (D11371), katB (D21137), and katC
(D21138); contains non-consensus AT-AC splice sites at
intron 10
Length = 1087
Score = 35.5 bits (78), Expect = 0.041
Identities = 19/39 (48%), Positives = 24/39 (61%), Gaps = 4/39 (10%)
Frame = -1
Query: 709 EPLPNGAYED----VLRDGVILCKLANKLAPGSVKKIQE 605
E LP ED LR+G+ILC + NK+ PGSV K+ E
Sbjct: 7 ETLPEKPSEDEFSLALRNGLILCNVLNKVNPGSVLKVVE 45
>At5g35700.1 68418.m04269 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893, fimbrin [Schizosaccharomyces pombe]
GI:3057144; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 687
Score = 34.3 bits (75), Expect = 0.094
Identities = 14/30 (46%), Positives = 22/30 (73%)
Frame = -1
Query: 709 EPLPNGAYEDVLRDGVILCKLANKLAPGSV 620
+P N A+ D+++DGV+LCKL N PG++
Sbjct: 149 DPATN-AFFDLVKDGVLLCKLINVAVPGTI 177
>At5g48460.1 68418.m05992 fimbrin-like protein, putative strong
similarity to fimbrin-like protein AtFim2 [Arabidopsis
thaliana] GI:2737926; contains Pfam profile PF00307:
Calponin homology (CH) domain
Length = 654
Score = 33.1 bits (72), Expect = 0.22
Identities = 19/44 (43%), Positives = 25/44 (56%)
Frame = -1
Query: 706 PLPNGAYEDVLRDGVILCKLANKLAPGSVKKIQEKGTNFQLMEN 575
P N +E V +DGV+LCKL N PG+ I E+ N + M N
Sbjct: 152 PSSNDLFE-VAKDGVLLCKLINVAVPGT---IDERAINTKSMLN 191
>At4g26700.1 68417.m03848 fimbrin-like protein (FIM1) identical to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893
Length = 687
Score = 33.1 bits (72), Expect = 0.22
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = -1
Query: 709 EPLPNGAYEDVLRDGVILCKLANKLAPGSV 620
+P N YE +++DGV+LCKL N PG++
Sbjct: 150 DPHSNQLYE-LVKDGVLLCKLINVAVPGTI 178
>At5g55400.1 68418.m06902 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 714
Score = 32.7 bits (71), Expect = 0.29
Identities = 14/30 (46%), Positives = 21/30 (70%)
Frame = -1
Query: 709 EPLPNGAYEDVLRDGVILCKLANKLAPGSV 620
+P N YE +++DGV+LCKL N PG++
Sbjct: 151 DPDSNDLYE-LVKDGVLLCKLINIAVPGTI 179
>At2g04750.1 68415.m00485 fimbrin-like protein, putative similar to
fimbrin-like protein (ATFIM1) [Arabidopsis thaliana]
GI:2905893; contains Pfam profile PF00307: Calponin
homology (CH) domain
Length = 652
Score = 32.3 bits (70), Expect = 0.38
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -1
Query: 706 PLPNGAYEDVLRDGVILCKLANKLAPGSV 620
P N + D+++DGV+LCKL N PG++
Sbjct: 144 PTTNALF-DLVKDGVLLCKLINIAVPGTI 171
>At5g62240.1 68418.m07815 expressed protein various predicted
proteins, Arabidopsis thaliana; expression supported by
MPSS
Length = 366
Score = 31.5 bits (68), Expect = 0.66
Identities = 19/55 (34%), Positives = 27/55 (49%)
Frame = -1
Query: 658 LCKLANKLAPGSVKKIQEKGTNFQLMENIQRFQAAIKKYGVPEEEIFQTADLFER 494
L K +AP V K+Q + TN Q E ++ K VP+E +TA+ ER
Sbjct: 161 LAKFGENVAPVLVSKLQNQDTNRQKQEAKVAHVSSRAKLTVPKEPNLRTAERSER 215
>At5g10050.1 68418.m01164 short-chain dehydrogenase/reductase (SDR)
family protein contains INTERPRO family IPR002198 short
chain dehydrogenase/reductase SDR family
Length = 279
Score = 29.5 bits (63), Expect = 2.7
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = -1
Query: 238 FSTLISVFVHGRIWLNDFFTFKIIL 164
+STL++V H +WL DFF ++++
Sbjct: 253 YSTLMAVMYHMPLWLKDFFQKQVLM 277
>At3g11170.1 68416.m01355 omega-3 fatty acid desaturase, chloroplast
(FAD7) (FADD) identical to omega-3 fatty acid
desaturase, chloroplast precursor SP:P46310 [Arabidopsis
thaliana (Mouse-ear cress)]; identical to Pfam profile
PF00487: Fatty acid desaturase; identical to cDNA
plastid fatty acid desaturase GI:809491
Length = 446
Score = 29.5 bits (63), Expect = 2.7
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +3
Query: 618 FTDPGANLLANLQRITPSLSTSSY 689
+T P +N L+N + PSLS+SSY
Sbjct: 18 YTTPRSNFLSNNNKFRPSLSSSSY 41
>At2g25730.1 68415.m03084 expressed protein
Length = 2464
Score = 29.1 bits (62), Expect = 3.5
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +3
Query: 333 RLSSALWARSCSSVKVRSFLSAI 401
R + LW+RSC S + SFLS I
Sbjct: 52 RFDNVLWSRSCPSPSLLSFLSTI 74
>At5g25475.2 68418.m03031 expressed protein
Length = 282
Score = 27.9 bits (59), Expect = 8.2
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +3
Query: 711 PKHQRSK*EPLVLGPCFWQAFCAGEIW 791
PK+ K + V PCFW++ G+ W
Sbjct: 3 PKNAIEKIQANVSKPCFWKSLSPGQTW 29
>At5g25475.1 68418.m03030 expressed protein
Length = 282
Score = 27.9 bits (59), Expect = 8.2
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +3
Query: 711 PKHQRSK*EPLVLGPCFWQAFCAGEIW 791
PK+ K + V PCFW++ G+ W
Sbjct: 3 PKNAIEKIQANVSKPCFWKSLSPGQTW 29
>At2g46510.1 68415.m05796 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain
Length = 566
Score = 27.9 bits (59), Expect = 8.2
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -3
Query: 680 CAKGRCDPLQIGQQIGSWISEKNPRERDQLPAHG 579
C + R L G I S + EK PR+R + PA+G
Sbjct: 356 CTEKRPVSLLAGAGIVSVVDEKRPRKRGRKPANG 389
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,430,715
Number of Sequences: 28952
Number of extensions: 425591
Number of successful extensions: 1160
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 1104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1157
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1785055200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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