Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0410
(832 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|... 33 0.050
SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyc... 30 0.46
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 28 1.4
SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyce... 28 1.4
SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces p... 27 2.5
SPAC10F6.02c |prp22||ATP-dependent RNA helicase Prp22|Schizosacc... 27 3.3
SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 27 4.3
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 25 10.0
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 25 10.0
SPAC8F11.06 |||nuclear envelope protein |Schizosaccharomyces pom... 25 10.0
>SPCC63.14 |||conserved fungal protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1184
Score = 33.1 bits (72), Expect = 0.050
Identities = 31/86 (36%), Positives = 44/86 (51%), Gaps = 6/86 (6%)
Frame = +2
Query: 404 RKGSGPKSETSEERAA--RLAKMSAYAAQRLANESPEQRATRLKRMSEYA----AKRLSS 565
R+ P ERAA R K A A+RL + E++ RLK+ E A KRL
Sbjct: 695 RRNVDPMVSELSERAAQERERKEQAKEAKRLKKLAKEEK--RLKKKEEKARKAEEKRLQK 752
Query: 566 ETREQRAIRLARMSAYAARRLANETP 643
E R + A +++R SA+A + +AN P
Sbjct: 753 E-RAKYAKQMSRESAHADQAIANTGP 777
>SPAC29E6.10c ||SPAC30.14c|kinetochore protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1085
Score = 29.9 bits (64), Expect = 0.46
Identities = 31/128 (24%), Positives = 53/128 (41%), Gaps = 8/128 (6%)
Frame = +2
Query: 314 EQETLNTVEPVDMIQEMDPLSLLEPKARRRRKGSGPKSETSEERAARLAKMSAYAAQRLA 493
+ + L +E + ++ L + K ++R K K EER R A+ A A + A
Sbjct: 569 QAKLLEEIEEENKRKQERELKKIREKEKKRDKKKQLKLAKEEERQRREAERLAEQAAQKA 628
Query: 494 NESPEQRATR-------LKRMSEYAAKRLSSETREQRAIRLARMSAYAARRL-ANETPAQ 649
E+ Q R LKR E + L + RE++ + R ++ A+
Sbjct: 629 LEAKRQEEARKKREEQRLKREQEKKQQELERQKREEKQKQKEREKKLKKQQQEADREKMA 688
Query: 650 RQARLLED 673
R+ RL E+
Sbjct: 689 REQRLREE 696
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 28.3 bits (60), Expect = 1.4
Identities = 15/61 (24%), Positives = 36/61 (59%), Gaps = 1/61 (1%)
Frame = +2
Query: 497 ESPEQRATRLKRM-SEYAAKRLSSETREQRAIRLARMSAYAARRLANETPAQRQARLLED 673
E Q+A +L++ ++ AAK+ E R+ + + A++++ A + TP ++ ++L+D
Sbjct: 31 ERERQKAAKLEKYHAKLAAKKAKEEARKPKLDKKAKIASPVAEYVEKTTPGEK--KVLQD 88
Query: 674 V 676
+
Sbjct: 89 L 89
>SPAC6G10.05c |||TRAPP complex subunit Trs120 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1210
Score = 28.3 bits (60), Expect = 1.4
Identities = 22/81 (27%), Positives = 37/81 (45%), Gaps = 3/81 (3%)
Frame = -2
Query: 606 DILANLIALCSLVSD-ESLLAAYSDIRFSLVARCSGDSFASLCAAYAD--ILASRAALSS 436
D+L LI+LC +SD S+L S + V S S+ A+ + AS A +
Sbjct: 541 DVLNELISLCDSLSDYRSILLLISLFFLTSVQTASSSQQISMFKAFRKTYLFASNAGIHI 600
Query: 435 DVSLFGPEPFLRLRALGSNND 373
+ + P L+ +GS+ +
Sbjct: 601 NAPYWDPFMITDLKFIGSSEN 621
>SPAC10F6.10 |||protein kinase, RIO family |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 521
Score = 27.5 bits (58), Expect = 2.5
Identities = 14/47 (29%), Positives = 24/47 (51%)
Frame = +2
Query: 398 RRRKGSGPKSETSEERAARLAKMSAYAAQRLANESPEQRATRLKRMS 538
++ KG+G ET EE+ AR K A++ ++ P+ R + S
Sbjct: 471 KQGKGNGRAKETPEEKRARKKKTKEDKAEKRKSKIPKYEKKRKLKQS 517
>SPAC10F6.02c |prp22||ATP-dependent RNA helicase
Prp22|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1168
Score = 27.1 bits (57), Expect = 3.3
Identities = 24/71 (33%), Positives = 35/71 (49%), Gaps = 7/71 (9%)
Frame = +2
Query: 467 SAYAAQRLANESPE--QRATRLKRMSEYAAKRLSSETREQRAIRLARMSAY-----AARR 625
+A Q LAN+ E Q+ +LK E + LS ++ + R A AAR+
Sbjct: 417 AAMQGQILANDRREIRQKEAKLKSEQEMEKQDLSLSWQDTMSNPQDRKFAQDVRDSAARQ 476
Query: 626 LANETPAQRQA 658
L +ETP+ RQA
Sbjct: 477 LTSETPSWRQA 487
>SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 444
Score = 26.6 bits (56), Expect = 4.3
Identities = 22/68 (32%), Positives = 30/68 (44%)
Frame = -2
Query: 600 LANLIALCSLVSDESLLAAYSDIRFSLVARCSGDSFASLCAAYADILASRAALSSDVSLF 421
+A + L +L +LL +YS SL C+GDS A L D LS D +
Sbjct: 195 VAASLLLPALSGSCALLTSYSAKSKSLQVACTGDSRAVLGECTPDGSWEAIPLSRDQTGM 254
Query: 420 GPEPFLRL 397
P+ RL
Sbjct: 255 NPDEASRL 262
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 25.4 bits (53), Expect = 10.0
Identities = 18/58 (31%), Positives = 26/58 (44%)
Frame = +2
Query: 476 AAQRLANESPEQRATRLKRMSEYAAKRLSSETREQRAIRLARMSAYAARRLANETPAQ 649
AAQR + E A L+ E + RE+ AIR+ A++LA E A+
Sbjct: 586 AAQRALKQKQESEAESLRVQEEINKRNAERIRREKEAIRINE-----AKKLAEELKAK 638
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 25.4 bits (53), Expect = 10.0
Identities = 27/106 (25%), Positives = 44/106 (41%), Gaps = 10/106 (9%)
Frame = +2
Query: 386 PKARRRRKGSGPKSETSEERAARLAKMSAYAAQRLANESPEQRATRLKRMSEYAA--KRL 559
PK R + + + EE+ + A+ ++ + EQ A + K E A +RL
Sbjct: 4 PKKRAEKAEAAKSRKQDEEKKKKDAEEDEKWSKGVKTNKKEQEAEKRKAALERKAERERL 63
Query: 560 SSETREQRAIRLARMSAYAARR------LANETP--AQRQARLLED 673
E E + + S AA++ NETP A AR ++D
Sbjct: 64 EKEEMESLPSKGGKGSKKAAKKNSSLDAFLNETPQTASYSARNIDD 109
>SPAC8F11.06 |||nuclear envelope protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 297
Score = 25.4 bits (53), Expect = 10.0
Identities = 15/49 (30%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Frame = +2
Query: 329 NTVEPVDMIQEMDPLSLLEPKARRRRKGSGPKSETSE--ERAARLAKMS 469
N EP+D+ D +L + RR+ + S P S +++ R L K S
Sbjct: 40 NDPEPMDISMSPDEKNLKKSTVRRKLRKSKPNSSSNQVSSRTRALTKRS 88
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,169,245
Number of Sequences: 5004
Number of extensions: 61521
Number of successful extensions: 166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 165
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 408446760
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -