Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0407
(680 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g15930.1 68417.m02419 dynein light chain, putative similar to... 138 2e-33
At4g27360.1 68417.m03927 dynein light chain, putative similar to... 91 6e-19
At3g16120.1 68416.m02036 dynein light chain, putative similar to... 86 2e-17
At1g52250.1 68414.m05895 dynein light chain type 1 family protei... 85 3e-17
At5g20110.1 68418.m02394 dynein light chain, putative similar to... 84 7e-17
At1g23220.1 68414.m02904 dynein light chain type 1 family protei... 80 2e-15
At5g35370.1 68418.m04204 lectin protein kinase family protein co... 29 2.2
At1g54680.1 68414.m06234 expressed protein 29 3.8
At1g05450.2 68414.m00553 protease inhibitor/seed storage/lipid t... 28 5.0
At3g02910.1 68416.m00286 expressed protein contains Pfam domain ... 27 8.7
>At4g15930.1 68417.m02419 dynein light chain, putative similar to
dynein light chain 2 [Mus musculus] GI:15545995;
contains Pfam profile PF01221: Dynein light chain type 1
Length = 103
Score = 138 bits (335), Expect = 2e-33
Identities = 59/86 (68%), Positives = 74/86 (86%)
Frame = +3
Query: 84 RKAVIKNADMSEEMQQDAVDCATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFG 263
++AVIK+ADM ++MQ++A++ A A EK+++EKDIA IKKEFDKK+ TWHCIVGRNFG
Sbjct: 18 KRAVIKSADMKDDMQKEAIEIAISAFEKYSVEKDIAENIKKEFDKKHGATWHCIVGRNFG 77
Query: 264 SYVTHETRHFIYFYLGQVAILLFKSG 341
SYVTHET HF+YFYL Q A+LLFKSG
Sbjct: 78 SYVTHETNHFVYFYLDQKAVLLFKSG 103
>At4g27360.1 68417.m03927 dynein light chain, putative similar to
SP|O02414 Dynein light chain LC6, flagellar outer arm
{Anthocidaris crassispina}; contains Pfam profile
PF01221: Dynein light chain type 1
Length = 103
Score = 91.1 bits (216), Expect = 6e-19
Identities = 43/89 (48%), Positives = 63/89 (70%), Gaps = 2/89 (2%)
Frame = +3
Query: 75 MCDRKAVIKNADMSEEMQQDAVDCATQALEKFNIEK--DIAAFIKKEFDKKYNPTWHCIV 248
M + KAV+ + DM + M++DA+ A++AL+ F++ + IA FIKKEFD+ Y W CIV
Sbjct: 1 MLEGKAVMGDTDMKQTMKEDALSLASKALDCFDVTEPTQIARFIKKEFDRSYGSGWQCIV 60
Query: 249 GRNFGSYVTHETRHFIYFYLGQVAILLFK 335
G +FGS+VTH + FI+F +G + ILLFK
Sbjct: 61 GTHFGSFVTHCSGCFIHFSVGSLTILLFK 89
>At3g16120.1 68416.m02036 dynein light chain, putative similar to
SP|O02414 Dynein light chain LC6, flagellar outer arm
{Anthocidaris crassispina}; contains Pfam profile
PF01221: Dynein light chain type 1
Length = 93
Score = 86.2 bits (204), Expect = 2e-17
Identities = 40/89 (44%), Positives = 57/89 (64%), Gaps = 2/89 (2%)
Frame = +3
Query: 75 MCDRKAVIKNADMSEEMQQDAVDCATQALEKFNI--EKDIAAFIKKEFDKKYNPTWHCIV 248
M + KA ++ DM +MQ A+ A+Q+L+ F++ IAA IKKEFD++Y W C+V
Sbjct: 1 MLEGKAKVEETDMPVKMQMQAMKIASQSLDLFDVFDSISIAAHIKKEFDERYGSGWQCVV 60
Query: 249 GRNFGSYVTHETRHFIYFYLGQVAILLFK 335
G NFG + TH FIYF+LG + L+FK
Sbjct: 61 GTNFGCFFTHSKGTFIYFHLGTLNFLIFK 89
>At1g52250.1 68414.m05895 dynein light chain type 1 family protein
similar to SP|O02414 Dynein light chain LC6, flagellar
outer arm {Anthocidaris crassispina}; contains Pfam
profile PF01221: Dynein light chain type 1
Length = 94
Score = 85.4 bits (202), Expect = 3e-17
Identities = 41/93 (44%), Positives = 59/93 (63%), Gaps = 2/93 (2%)
Frame = +3
Query: 75 MCDRKAVIKNADMSEEMQQDAVDCATQALEKFNIE--KDIAAFIKKEFDKKYNPTWHCIV 248
M + KA+++++DM +MQ A+ A+QAL+ F++ K IA IKKEFD++Y W C+V
Sbjct: 1 MLEGKAMVEDSDMPVKMQMQAMAFASQALDLFDVFDCKSIAGHIKKEFDERYGSGWQCVV 60
Query: 249 GRNFGSYVTHETRHFIYFYLGQVAILLFKSG*T 347
G NFG + TH FIYF L + L+FK T
Sbjct: 61 GSNFGCFFTHSKGTFIYFQLETLKFLIFKGAST 93
>At5g20110.1 68418.m02394 dynein light chain, putative similar to
SP|O02414 Dynein light chain LC6, flagellar outer arm
{Anthocidaris crassispina}; contains Pfam profile
PF01221: Dynein light chain type 1
Length = 209
Score = 84.2 bits (199), Expect = 7e-17
Identities = 43/81 (53%), Positives = 54/81 (66%), Gaps = 3/81 (3%)
Frame = +3
Query: 105 ADMSEEMQQDAVDCATQ---ALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVT 275
ADM MQ A CA +LEKF+ K +A +KKEFDK Y P WHCIVG +FGS+VT
Sbjct: 122 ADMPGFMQAHAFRCARMTLDSLEKFS-SKHMAFNLKKEFDKGYGPAWHCIVGSSFGSFVT 180
Query: 276 HETRHFIYFYLGQVAILLFKS 338
H T FIYF + ++ +LLFK+
Sbjct: 181 HSTGCFIYFSMDKLYVLLFKT 201
>At1g23220.1 68414.m02904 dynein light chain type 1 family protein
similar to SP|O02414 Dynein light chain LC6, flagellar
outer arm {Anthocidaris crassispina}; contains Pfam
profile PF01221: Dynein light chain type 1
Length = 129
Score = 79.8 bits (188), Expect = 2e-15
Identities = 39/103 (37%), Positives = 58/103 (56%), Gaps = 3/103 (2%)
Frame = +3
Query: 39 VHLSKQKQTQDKMCDRKAVIKNADMSEEMQQDAVDCATQALEKFNIEKD---IAAFIKKE 209
+ K K+ QD+ + ++ +DM Q A + + L + D +A +KK+
Sbjct: 19 IQKKKAKEQQDQKDEFNVRVRASDMPLPQQNRAFSLSREILNATPGKADNKRLAHALKKD 78
Query: 210 FDKKYNPTWHCIVGRNFGSYVTHETRHFIYFYLGQVAILLFKS 338
FD Y P WHCIVG +FGSYVTH T F+YF + +V +LLFK+
Sbjct: 79 FDSAYGPAWHCIVGTSFGSYVTHSTGGFLYFQIDKVYVLLFKT 121
>At5g35370.1 68418.m04204 lectin protein kinase family protein
contains Pfam domains, PF01453: Lectin (probable mannose
binding) and PF00069: Protein kinase domain
Length = 870
Score = 29.5 bits (63), Expect = 2.2
Identities = 23/69 (33%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = -2
Query: 340 PLLKSSIATCPR*K*MKWRVSCVTYEPKLRPTMQCQVGLYFLSNSFLMNAAM-SFSMLNF 164
P L+ + + K ++ + CV EP LRPTM VG+ F + L N M S + L F
Sbjct: 762 PRLEGRVTSQEAEKLVRIALCCVHEEPALRPTMAAVVGM-FEGSIPLGNPRMESLNFLRF 820
Query: 163 SSA*VAQST 137
A+S+
Sbjct: 821 YGLRFAESS 829
>At1g54680.1 68414.m06234 expressed protein
Length = 289
Score = 28.7 bits (61), Expect = 3.8
Identities = 20/75 (26%), Positives = 38/75 (50%), Gaps = 1/75 (1%)
Frame = +3
Query: 87 KAVIKNADMSEEMQ-QDAVDCATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFG 263
K + K DMS + + +D ++++E +++D + KE + K +P ++
Sbjct: 63 KLLPKKLDMSSKTDLRSLIDSVSRSIESVYVQED-SVRTSKEMEIKTSPE------EDWF 115
Query: 264 SYVTHETRHFIYFYL 308
S V HE+ HF+ YL
Sbjct: 116 SVVQHESGHFLVGYL 130
>At1g05450.2 68414.m00553 protease inhibitor/seed storage/lipid
transfer protein (LTP)-related similar to geranyl
diphosphate synthase large subunit [Mentha x piperita]
GI:6449052
Length = 205
Score = 28.3 bits (60), Expect = 5.0
Identities = 17/38 (44%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = -2
Query: 214 SNSFLMNAAMSFSML--NFSSA*VAQSTASCCISSLMS 107
SNSFL++AA+ FS+L N ++ +AQ C S L S
Sbjct: 3 SNSFLISAALIFSLLSSNSPTSILAQINTPCSPSMLSS 40
>At3g02910.1 68416.m00286 expressed protein contains Pfam domain
PF03674: Uncharacterised protein family (UPF0131)
Length = 187
Score = 27.5 bits (58), Expect = 8.7
Identities = 16/61 (26%), Positives = 32/61 (52%)
Frame = +3
Query: 114 SEEMQQDAVDCATQALEKFNIEKDIAAFIKKEFDKKYNPTWHCIVGRNFGSYVTHETRHF 293
+EE +++ D T+A +E A + K ++++ W GR+FG+Y +E R +
Sbjct: 109 AEEEEEEEGDLETEAPSSCVVE---AYYAHKSYEEEL---WRRNRGRSFGAYTENEARGY 162
Query: 294 I 296
+
Sbjct: 163 V 163
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,789,224
Number of Sequences: 28952
Number of extensions: 263042
Number of successful extensions: 711
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 683
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 709
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1438152744
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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