BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0400
(839 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 36 0.033
At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 35 0.059
At1g35515.1 68414.m04409 myb family transcription factor (MYB8) ... 30 1.7
At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6... 29 5.1
At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) simi... 29 5.1
At5g54110.1 68418.m06737 vesicle-associated membrane family prot... 28 6.7
At5g45270.1 68418.m05556 WRKY family transcription factor contai... 28 6.7
At5g02290.2 68418.m00153 protein kinase, putative similar to pro... 28 8.9
At5g02290.1 68418.m00152 protein kinase, putative similar to pro... 28 8.9
At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-conta... 28 8.9
At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica... 28 8.9
At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 28 8.9
>At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein
(HSP17.8-CI) similar to 17.5 kDa class I heat shock
protein SP:P04793 from [Glycine max]; contains Pfam
PF00011: Hsp20/alpha crystallin family
Length = 157
Score = 35.9 bits (79), Expect = 0.033
Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 9/77 (11%)
Frame = +3
Query: 441 DVSQYTPEEIVVKTVDNKLLV-----HAKHEEKSDT-KSVYR---EYNREFLLPKGTNPE 593
D+ EE+ V+ D+ +L H + EEK DT V R +++R+F LP+ +
Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123
Query: 594 AIKSSLSRDGVLTVEAP 644
+K+S+ +GVLTV P
Sbjct: 124 QVKASM-ENGVLTVTVP 139
>At4g27670.1 68417.m03979 25.3 kDa small heat shock protein,
chloroplast precursor (HSP25.3-P) identical to small
heat shock protein, chloroplast precursor SP:P31170 from
[Arabidopsis thaliana]; identified in Scharf, K-D., et
al,Cell Stress & Chaperones (2001) 6: 225-237.
Length = 227
Score = 35.1 bits (77), Expect = 0.059
Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 4/77 (5%)
Frame = +3
Query: 426 LKLRFDVSQYTPEEIVVKTVDNKLLV--HAKHEEKSDTKS--VYREYNREFLLPKGTNPE 593
+K+RFD+ + E++ + DN L++ K E+ D+ S Y LP +
Sbjct: 138 IKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKD 197
Query: 594 AIKSSLSRDGVLTVEAP 644
IK+ L ++GVL + P
Sbjct: 198 KIKAEL-KNGVLFITIP 213
>At1g35515.1 68414.m04409 myb family transcription factor (MYB8)
similar to DNA-binding protein GB:AAA98761 GI:1020155
from [Arabidopsis thaliana]
Length = 212
Score = 30.3 bits (65), Expect = 1.7
Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 4/65 (6%)
Frame = +1
Query: 493 NYWSMPNTRRNLIRNLCTENTTGSFCCPREQILRP----LSLRCPGTVCLPWKRHCHNSP 660
NYW+ + +R L+ T GS P+ P L PG+V L + C N
Sbjct: 106 NYWNT-HIKRKLLNRGIDPKTHGSIIEPKTTSFHPRNEDLKSTFPGSVKLKMETSCENCA 164
Query: 661 SRTGT 675
S +GT
Sbjct: 165 STSGT 169
>At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6) /
HD-ZIP transcription factor 6 identical to
homeobox-leucine zipper protein ATHB-6 (HD-ZIP protein
ATHB-6) (SP:P46668) [Arabidopsis thaliana]
Length = 311
Score = 28.7 bits (61), Expect = 5.1
Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Frame = +3
Query: 177 DFSVIDTEFSSIRERFDAEMRKME---EEMSKFRSEL 278
D+ V+ T++ S+R FD+ R E +E+SK +++L
Sbjct: 122 DYGVLKTQYDSLRHNFDSLRRDNESLLQEISKLKTKL 158
>At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) similar
to CREB-binding protein GB:AAC51770 GI:2443859 from
[Homo sapiens]; contains Pfam PF02135: TAZ zinc finger
profile; contains Pfam PF00569: Zinc finger, ZZ type
domain; identical to histone acetyltransferase HAC4
(GI:14794966) {Arabidopsis thaliana}
Length = 1456
Score = 28.7 bits (61), Expect = 5.1
Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Frame = +1
Query: 412 VTARLSSFALMSASILPKRSLLR-LSTTNYWSMPNTRRNL 528
VT+ S+ M + LPKR L L TN+ S PN R NL
Sbjct: 315 VTSYHSNLQPMQQTPLPKRQLHHPLWNTNFQSAPNNRDNL 354
>At5g54110.1 68418.m06737 vesicle-associated membrane family protein
/ VAMP family protein similar to VAP27 GI:6688926
[Nicotiana plumbaginifolia]
Length = 266
Score = 28.3 bits (60), Expect = 6.7
Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 3/67 (4%)
Frame = +1
Query: 442 MSASILPKRSLLRLSTTNYWSMPNTRRNLIRN-LCTENTTGSFCCPREQILRPLS--LRC 612
++ S+LP R LRL ++Y P +R+ + +NT+ S + Q P S +R
Sbjct: 66 VARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRP 125
Query: 613 PGTVCLP 633
PG V P
Sbjct: 126 PGGVLAP 132
>At5g45270.1 68418.m05556 WRKY family transcription factor contains
Pfam profile: PF03106 WRKY DNA -binding domain
Length = 114
Score = 28.3 bits (60), Expect = 6.7
Identities = 10/30 (33%), Positives = 17/30 (56%)
Frame = -1
Query: 668 VRDGELWQWRFHGKHTVPGQRRLNGLRICS 579
+ +G+LW WR +G+ + G R G C+
Sbjct: 31 IDEGDLWTWRKYGQKDILGSRFPRGYYRCA 60
>At5g02290.2 68418.m00153 protein kinase, putative similar to
protein kinase APK1A [Arabidopsis thaliana]
SWISS-PROT:Q06548
Length = 389
Score = 27.9 bits (59), Expect = 8.9
Identities = 24/103 (23%), Positives = 44/103 (42%), Gaps = 3/103 (2%)
Frame = +1
Query: 400 FKTRVTARLSSFALMSASILPKRSLLRLSTTNYWSMPNTRRNLIRNLCTENTTGSFCCPR 579
F R+ ++S +S+ L + ST ++ MP T +++N +N + S
Sbjct: 5 FSNRIKTDIASSTWLSSKFLSRDGSKGSSTASFSYMPRTEGEILQNANLKNFSLSELKSA 64
Query: 580 EQILRPLSLRCP---GTVCLPWKRHCHNSPSRTGTFLSRSTER 699
+ RP S+ G V W +PS+ GT + + +R
Sbjct: 65 TRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKR 107
>At5g02290.1 68418.m00152 protein kinase, putative similar to
protein kinase APK1A [Arabidopsis thaliana]
SWISS-PROT:Q06548
Length = 389
Score = 27.9 bits (59), Expect = 8.9
Identities = 24/103 (23%), Positives = 44/103 (42%), Gaps = 3/103 (2%)
Frame = +1
Query: 400 FKTRVTARLSSFALMSASILPKRSLLRLSTTNYWSMPNTRRNLIRNLCTENTTGSFCCPR 579
F R+ ++S +S+ L + ST ++ MP T +++N +N + S
Sbjct: 5 FSNRIKTDIASSTWLSSKFLSRDGSKGSSTASFSYMPRTEGEILQNANLKNFSLSELKSA 64
Query: 580 EQILRPLSLRCP---GTVCLPWKRHCHNSPSRTGTFLSRSTER 699
+ RP S+ G V W +PS+ GT + + +R
Sbjct: 65 TRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKR 107
>At1g79940.1 68414.m09342 DNAJ heat shock N-terminal
domain-containing protein / sec63 domain-containing
protein similar to SP|Q9UGP8 Translocation protein SEC63
homolog {Homo sapiens}; contains Pfam profiles PF00226
DnaJ domain, PF02889 Sec63 domain
Length = 702
Score = 27.9 bits (59), Expect = 8.9
Identities = 10/36 (27%), Positives = 21/36 (58%)
Frame = +3
Query: 159 IPIKLGDFSVIDTEFSSIRERFDAEMRKMEEEMSKF 266
IP++ D + F S+R + +++ M++E +KF
Sbjct: 258 IPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKF 293
>At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 662
Score = 27.9 bits (59), Expect = 8.9
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +3
Query: 453 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 536
YTPEE+ + NKL V+A+ + K+D +
Sbjct: 619 YTPEEVKKLSPKNKLAVNARMQLKADAE 646
>At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical
to auxin response factor 1 GI:2245378 from [Arabidopsis
thaliana]
Length = 665
Score = 27.9 bits (59), Expect = 8.9
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +3
Query: 453 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 536
YTPEE+ + NKL V+A+ + K+D +
Sbjct: 622 YTPEEVKKLSPKNKLAVNARMQLKADAE 649
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,309,795
Number of Sequences: 28952
Number of extensions: 341001
Number of successful extensions: 1183
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 1122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1183
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1941125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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