BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0400 (839 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HS... 36 0.033 At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chlo... 35 0.059 At1g35515.1 68414.m04409 myb family transcription factor (MYB8) ... 30 1.7 At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6... 29 5.1 At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) simi... 29 5.1 At5g54110.1 68418.m06737 vesicle-associated membrane family prot... 28 6.7 At5g45270.1 68418.m05556 WRKY family transcription factor contai... 28 6.7 At5g02290.2 68418.m00153 protein kinase, putative similar to pro... 28 8.9 At5g02290.1 68418.m00152 protein kinase, putative similar to pro... 28 8.9 At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-conta... 28 8.9 At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identica... 28 8.9 At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identica... 28 8.9 >At1g07400.1 68414.m00789 17.8 kDa class I heat shock protein (HSP17.8-CI) similar to 17.5 kDa class I heat shock protein SP:P04793 from [Glycine max]; contains Pfam PF00011: Hsp20/alpha crystallin family Length = 157 Score = 35.9 bits (79), Expect = 0.033 Identities = 26/77 (33%), Positives = 42/77 (54%), Gaps = 9/77 (11%) Frame = +3 Query: 441 DVSQYTPEEIVVKTVDNKLLV-----HAKHEEKSDT-KSVYR---EYNREFLLPKGTNPE 593 D+ EE+ V+ D+ +L H + EEK DT V R +++R+F LP+ + Sbjct: 64 DLPGMKKEEVKVEIEDDSVLKISGERHVEKEEKQDTWHRVERSSGQFSRKFKLPENVKMD 123 Query: 594 AIKSSLSRDGVLTVEAP 644 +K+S+ +GVLTV P Sbjct: 124 QVKASM-ENGVLTVTVP 139 >At4g27670.1 68417.m03979 25.3 kDa small heat shock protein, chloroplast precursor (HSP25.3-P) identical to small heat shock protein, chloroplast precursor SP:P31170 from [Arabidopsis thaliana]; identified in Scharf, K-D., et al,Cell Stress & Chaperones (2001) 6: 225-237. Length = 227 Score = 35.1 bits (77), Expect = 0.059 Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 4/77 (5%) Frame = +3 Query: 426 LKLRFDVSQYTPEEIVVKTVDNKLLV--HAKHEEKSDTKS--VYREYNREFLLPKGTNPE 593 +K+RFD+ + E++ + DN L++ K E+ D+ S Y LP + Sbjct: 138 IKMRFDMPGLSKEDVKISVEDNVLVIKGEQKKEDSDDSWSGRSVSSYGTRLQLPDNCEKD 197 Query: 594 AIKSSLSRDGVLTVEAP 644 IK+ L ++GVL + P Sbjct: 198 KIKAEL-KNGVLFITIP 213 >At1g35515.1 68414.m04409 myb family transcription factor (MYB8) similar to DNA-binding protein GB:AAA98761 GI:1020155 from [Arabidopsis thaliana] Length = 212 Score = 30.3 bits (65), Expect = 1.7 Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 4/65 (6%) Frame = +1 Query: 493 NYWSMPNTRRNLIRNLCTENTTGSFCCPREQILRP----LSLRCPGTVCLPWKRHCHNSP 660 NYW+ + +R L+ T GS P+ P L PG+V L + C N Sbjct: 106 NYWNT-HIKRKLLNRGIDPKTHGSIIEPKTTSFHPRNEDLKSTFPGSVKLKMETSCENCA 164 Query: 661 SRTGT 675 S +GT Sbjct: 165 STSGT 169 >At2g22430.1 68415.m02660 homeobox-leucine zipper protein 6 (HB-6) / HD-ZIP transcription factor 6 identical to homeobox-leucine zipper protein ATHB-6 (HD-ZIP protein ATHB-6) (SP:P46668) [Arabidopsis thaliana] Length = 311 Score = 28.7 bits (61), Expect = 5.1 Identities = 13/37 (35%), Positives = 24/37 (64%), Gaps = 3/37 (8%) Frame = +3 Query: 177 DFSVIDTEFSSIRERFDAEMRKME---EEMSKFRSEL 278 D+ V+ T++ S+R FD+ R E +E+SK +++L Sbjct: 122 DYGVLKTQYDSLRHNFDSLRRDNESLLQEISKLKTKL 158 >At1g55970.1 68414.m06419 histone acetyltransferase 4 (HAC4) similar to CREB-binding protein GB:AAC51770 GI:2443859 from [Homo sapiens]; contains Pfam PF02135: TAZ zinc finger profile; contains Pfam PF00569: Zinc finger, ZZ type domain; identical to histone acetyltransferase HAC4 (GI:14794966) {Arabidopsis thaliana} Length = 1456 Score = 28.7 bits (61), Expect = 5.1 Identities = 18/40 (45%), Positives = 22/40 (55%), Gaps = 1/40 (2%) Frame = +1 Query: 412 VTARLSSFALMSASILPKRSLLR-LSTTNYWSMPNTRRNL 528 VT+ S+ M + LPKR L L TN+ S PN R NL Sbjct: 315 VTSYHSNLQPMQQTPLPKRQLHHPLWNTNFQSAPNNRDNL 354 >At5g54110.1 68418.m06737 vesicle-associated membrane family protein / VAMP family protein similar to VAP27 GI:6688926 [Nicotiana plumbaginifolia] Length = 266 Score = 28.3 bits (60), Expect = 6.7 Identities = 21/67 (31%), Positives = 33/67 (49%), Gaps = 3/67 (4%) Frame = +1 Query: 442 MSASILPKRSLLRLSTTNYWSMPNTRRNLIRN-LCTENTTGSFCCPREQILRPLS--LRC 612 ++ S+LP R LRL ++Y P +R+ + +NT+ S + Q P S +R Sbjct: 66 VARSLLPARRRLRLDPSSYLYFPYEPGKQVRSAIKLKNTSKSHTAFKFQTTAPKSCYMRP 125 Query: 613 PGTVCLP 633 PG V P Sbjct: 126 PGGVLAP 132 >At5g45270.1 68418.m05556 WRKY family transcription factor contains Pfam profile: PF03106 WRKY DNA -binding domain Length = 114 Score = 28.3 bits (60), Expect = 6.7 Identities = 10/30 (33%), Positives = 17/30 (56%) Frame = -1 Query: 668 VRDGELWQWRFHGKHTVPGQRRLNGLRICS 579 + +G+LW WR +G+ + G R G C+ Sbjct: 31 IDEGDLWTWRKYGQKDILGSRFPRGYYRCA 60 >At5g02290.2 68418.m00153 protein kinase, putative similar to protein kinase APK1A [Arabidopsis thaliana] SWISS-PROT:Q06548 Length = 389 Score = 27.9 bits (59), Expect = 8.9 Identities = 24/103 (23%), Positives = 44/103 (42%), Gaps = 3/103 (2%) Frame = +1 Query: 400 FKTRVTARLSSFALMSASILPKRSLLRLSTTNYWSMPNTRRNLIRNLCTENTTGSFCCPR 579 F R+ ++S +S+ L + ST ++ MP T +++N +N + S Sbjct: 5 FSNRIKTDIASSTWLSSKFLSRDGSKGSSTASFSYMPRTEGEILQNANLKNFSLSELKSA 64 Query: 580 EQILRPLSLRCP---GTVCLPWKRHCHNSPSRTGTFLSRSTER 699 + RP S+ G V W +PS+ GT + + +R Sbjct: 65 TRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKR 107 >At5g02290.1 68418.m00152 protein kinase, putative similar to protein kinase APK1A [Arabidopsis thaliana] SWISS-PROT:Q06548 Length = 389 Score = 27.9 bits (59), Expect = 8.9 Identities = 24/103 (23%), Positives = 44/103 (42%), Gaps = 3/103 (2%) Frame = +1 Query: 400 FKTRVTARLSSFALMSASILPKRSLLRLSTTNYWSMPNTRRNLIRNLCTENTTGSFCCPR 579 F R+ ++S +S+ L + ST ++ MP T +++N +N + S Sbjct: 5 FSNRIKTDIASSTWLSSKFLSRDGSKGSSTASFSYMPRTEGEILQNANLKNFSLSELKSA 64 Query: 580 EQILRPLSLRCP---GTVCLPWKRHCHNSPSRTGTFLSRSTER 699 + RP S+ G V W +PS+ GT + + +R Sbjct: 65 TRNFRPDSVVGEGGFGCVFKGWIDESSLAPSKPGTGIVIAVKR 107 >At1g79940.1 68414.m09342 DNAJ heat shock N-terminal domain-containing protein / sec63 domain-containing protein similar to SP|Q9UGP8 Translocation protein SEC63 homolog {Homo sapiens}; contains Pfam profiles PF00226 DnaJ domain, PF02889 Sec63 domain Length = 702 Score = 27.9 bits (59), Expect = 8.9 Identities = 10/36 (27%), Positives = 21/36 (58%) Frame = +3 Query: 159 IPIKLGDFSVIDTEFSSIRERFDAEMRKMEEEMSKF 266 IP++ D + F S+R + +++ M++E +KF Sbjct: 258 IPVRRTDDEPLQKLFMSVRSELNLDLKNMKQEQAKF 293 >At1g59750.2 68414.m06728 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 662 Score = 27.9 bits (59), Expect = 8.9 Identities = 12/28 (42%), Positives = 19/28 (67%) Frame = +3 Query: 453 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 536 YTPEE+ + NKL V+A+ + K+D + Sbjct: 619 YTPEEVKKLSPKNKLAVNARMQLKADAE 646 >At1g59750.1 68414.m06727 auxin-responsive factor (ARF1) identical to auxin response factor 1 GI:2245378 from [Arabidopsis thaliana] Length = 665 Score = 27.9 bits (59), Expect = 8.9 Identities = 12/28 (42%), Positives = 19/28 (67%) Frame = +3 Query: 453 YTPEEIVVKTVDNKLLVHAKHEEKSDTK 536 YTPEE+ + NKL V+A+ + K+D + Sbjct: 622 YTPEEVKKLSPKNKLAVNARMQLKADAE 649 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 16,309,795 Number of Sequences: 28952 Number of extensions: 341001 Number of successful extensions: 1183 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 1122 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1183 length of database: 12,070,560 effective HSP length: 80 effective length of database: 9,754,400 effective search space used: 1941125600 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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