Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0387
(767 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomy... 81 1e-16
SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces ... 71 2e-13
SPBC17D11.07c |rpn2||19S proteasome regulatory subunit Rpn2|Schi... 30 0.32
SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|... 28 1.7
SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase Abc4|... 27 3.9
SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces... 27 3.9
SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3 |Schizos... 26 6.8
SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces... 26 6.8
SPBC776.09 |ste13||ATP-dependent RNA helicase Ste13|Schizosaccha... 25 9.0
SPAC22E12.16c |pik1||phosphatidylinositol kinase Pik1|Schizosacc... 25 9.0
SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces pom... 25 9.0
>SPAC19A8.04 |erg5||C-22 sterol desaturase Erg5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 541
Score = 81.4 bits (192), Expect = 1e-16
Identities = 47/163 (28%), Positives = 79/163 (48%), Gaps = 1/163 (0%)
Frame = +2
Query: 89 SDEEIKEQVDTIMFEGHDTTAAGSSFFLSMMGIHQDIQDKVIEELDQIF-GDSDRPVTFQ 265
SDEEI + +F D T++ ++ ++ H D+ KV EE +I GD D P++
Sbjct: 305 SDEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVREEQLRIRKGDIDVPLSLD 364
Query: 266 DTLEMKYLERCLMETLRLYPPVPIIARQVNQEITLPSNGKKIPAGTTLVIATYKLHRRPD 445
+M Y + E LRL PPV ++ +V + + + +P ++ Y
Sbjct: 365 LMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPITPD-YTVPKDAMVIPTLYGALHDSK 423
Query: 446 VYPNPNKFDPDNFLPERSANRHYYAFVPFSAGPRSCVGRKYAM 574
VYP P F+PD + P A + ++ F GP C+G++YA+
Sbjct: 424 VYPEPETFNPDRWAPNGLAEQSPKNWMVFGNGPHVCLGQRYAV 466
>SPAC13A11.02c |erg11||sterol 14-demethylase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 495
Score = 70.9 bits (166), Expect = 2e-13
Identities = 47/197 (23%), Positives = 93/197 (47%), Gaps = 15/197 (7%)
Frame = +2
Query: 71 QSGVAISDEEIKEQVDTIMFEGHDTTAAGSSFFLSMMGIHQDIQDKVIEELDQIFGDSDR 250
+ G + + EI + ++ G T+AA + L+++G +I + + EE ++ G++
Sbjct: 268 RDGRPLKEHEIAGMMIALLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGEN-L 326
Query: 251 PVTFQDTLEMKYLERCLMETLRLYPPVPIIARQVNQEITLPSNGKKIPAGTTLVIATYKL 430
+ F +M L + ETLRL+PP+ R+V +++ +P + IPA L+ A
Sbjct: 327 ELKFDQYKDMPLLNYVIQETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLT 386
Query: 431 HRRPDVYPNPNKFDP----DNFLPERSANRHYYA-----------FVPFSAGPRSCVGRK 565
+ + + FDP D + +A + Y ++PF AG C+G +
Sbjct: 387 ATEEEYFTHATDFDPKRWNDRVNEDENAEQIDYGYGLVTKGAASPYLPFGAGRHRCIGEQ 446
Query: 566 YAMLKLKVILSTILRNF 616
+A + L I+S + ++
Sbjct: 447 FAYMHLSTIISKFVHDY 463
>SPBC17D11.07c |rpn2||19S proteasome regulatory subunit
Rpn2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 965
Score = 30.3 bits (65), Expect = 0.32
Identities = 18/42 (42%), Positives = 25/42 (59%)
Frame = +2
Query: 53 LLLESSQSGVAISDEEIKEQVDTIMFEGHDTTAAGSSFFLSM 178
LLL +G+A DE + E V TI+F +D AGS+ +SM
Sbjct: 460 LLLGIGLTGMASRDETLYESVKTILF--NDNAVAGSAAGISM 499
>SPCC736.07c |||cell polarity protein |Schizosaccharomyces pombe|chr
3|||Manual
Length = 699
Score = 27.9 bits (59), Expect = 1.7
Identities = 22/90 (24%), Positives = 42/90 (46%), Gaps = 2/90 (2%)
Frame = +2
Query: 62 ESSQSGVAIS-DEEIKEQVDTIMFEGHDTTAAGSSFFLSMMGIHQDIQD-KVIEELDQIF 235
E + G+ I +E+K D F T G++ +++ ++ D I E +++
Sbjct: 424 EEDEHGMTIGFSKELKAPPDPQYF----TEKTGATTYVNGNDESLNVSDFPQIVEQEELS 479
Query: 236 GDSDRPVTFQDTLEMKYLERCLMETLRLYP 325
+ R V F DTLE+K++ R + + P
Sbjct: 480 SKNPRKVHFSDTLEIKHVSRSGKANIEVIP 509
>SPAC30.04c |abc4||glutathione S-conjugate-exporting ATPase
Abc4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1469
Score = 26.6 bits (56), Expect = 3.9
Identities = 23/70 (32%), Positives = 28/70 (40%)
Frame = -2
Query: 586 LQFEHCVFTSNATSWSS*KRHERIIMSVGGTFRKEVVRVKFVWIWVNVRAAMKFICSYHQ 407
L F V W+S K H+ ++ SV GTF + I V K I S
Sbjct: 950 LDFLRTVSYDRGAWWASRKLHDSMLESVFGTFASWFDKTPTGRI---VNRFAKDIRSIDM 1006
Query: 406 CCSGWDFLSI 377
SGW F SI
Sbjct: 1007 NLSGWLFFSI 1016
>SPAC1834.08 |mak1|phk3|histidine kinase Mak1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1639
Score = 26.6 bits (56), Expect = 3.9
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = -3
Query: 156 PAAVVS*PSNIIVSTCSLISSSEMATPLCELSSNRSRKAKRFFCPT 19
P A++S SN++ +LI+S E P S + S+ CPT
Sbjct: 520 PIAILSFKSNLVPYPENLIASIERLIPFIFSSYSNSQSVPLLPCPT 565
>SPAC1687.22c |puf3|SPAC222.02c|RNA-binding protein Puf3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 732
Score = 25.8 bits (54), Expect = 6.8
Identities = 12/29 (41%), Positives = 16/29 (55%)
Frame = +2
Query: 626 SDLKESDFKLQADIISETGRRIPSTLTAP 712
SDL+ +L + SE G R P TL +P
Sbjct: 166 SDLRPLKTELYGQLNSECGARFPQTLKSP 194
>SPBC15D4.15 |pho2||4-nitrophenylphosphatase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 298
Score = 25.8 bits (54), Expect = 6.8
Identities = 16/64 (25%), Positives = 28/64 (43%), Gaps = 1/64 (1%)
Frame = +2
Query: 272 LEMKYLERCLMETLRLYPPVPIIARQVNQEITLPSNGKKIP-AGTTLVIATYKLHRRPDV 448
+ + YL+ C+ P + NQ+ T P+NGK +P +G + R+P +
Sbjct: 160 MHVTYLKYCMAFQYLQDPNCAFLL--TNQDSTFPTNGKFLPGSGAISYPLIFSTGRQPKI 217
Query: 449 YPNP 460
P
Sbjct: 218 LGKP 221
>SPBC776.09 |ste13||ATP-dependent RNA helicase
Ste13|Schizosaccharomyces pombe|chr 2|||Manual
Length = 485
Score = 25.4 bits (53), Expect = 9.0
Identities = 10/32 (31%), Positives = 15/32 (46%)
Frame = -1
Query: 548 FLVQLKKARTHNNVCWRNVPEGSCQGQICLDL 453
F K ++H N + N G C+ +C DL
Sbjct: 309 FYSHAKMLQSHRNRVFHNFRNGVCRNLVCSDL 340
>SPAC22E12.16c |pik1||phosphatidylinositol kinase
Pik1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 851
Score = 25.4 bits (53), Expect = 9.0
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -1
Query: 209 LCLVYLDEYPSLIGRNYF 156
LC+ YL YP+ IG ++F
Sbjct: 24 LCVAYLSRYPNNIGIHHF 41
>SPAC3A12.02 |||inorganic pyrophosphatase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 286
Score = 25.4 bits (53), Expect = 9.0
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = +3
Query: 9 LTMMSDKRNVWPSLICCWRVHKVVWPFPMRKSKNK 113
L +SD +N+ P L+ C R ++ P K KN+
Sbjct: 175 LNDISDVQNLMPRLLPCTRDWFAIYKIPDGKPKNR 209
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,403,531
Number of Sequences: 5004
Number of extensions: 77824
Number of successful extensions: 205
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 202
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 369323696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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