SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0374
         (767 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g45220.1 68416.m04880 serpin, putative / serine protease inhi...    92   3e-19
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi...    91   7e-19
At1g47710.1 68414.m05302 serpin, putative / serine protease inhi...    85   4e-17
At2g25240.1 68415.m03020 serpin, putative / serine protease inhi...    83   3e-16
At1g64030.1 68414.m07252 serpin family protein / serine protease...    82   3e-16
At1g62170.1 68414.m07013 serpin family protein / serine protease...    77   1e-14
At2g14540.1 68415.m01628 serpin family protein / serine protease...    75   4e-14
At2g35580.1 68415.m04357 serpin family protein / serine protease...    69   5e-12
At1g51330.1 68414.m05772 serpin-related / serine protease inhibi...    64   7e-11
At1g63280.1 68414.m07154 serpin-related / serine protease inhibi...    64   1e-10
At3g15920.1 68416.m02013 phox (PX) domain-containing protein wea...    28   6.0  
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim...    28   7.9  
At1g72410.1 68414.m08374 COP1-interacting protein-related simila...    28   7.9  

>At3g45220.1 68416.m04880 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 393

 Score = 92.3 bits (219), Expect = 3e-19
 Identities = 58/193 (30%), Positives = 97/193 (50%), Gaps = 10/193 (5%)
 Frame = +3

Query: 156 IIQGKSVVLSAFSVLPPLAQLALASDGETHEELLKAIGFPDDDAIRTEFASKSRDLRSIK 335
           +  G ++V S  S+   L  +A  S+  T E++L  I  P  D +    A K+  +    
Sbjct: 25  VANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLA-KTVSVALND 83

Query: 336 GVE-----LKMANKVYVHDGGKLDENFAVVSRDVFNSDVQNIDFS-KNTVAAKSINDWVE 497
           G+E     L  A  V++        +F  +  + +N+    +DF+ K       +N W E
Sbjct: 84  GMERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFATKPAEVINEVNAWAE 143

Query: 498 ENTNNRIKDLVNPDSLSSATAAVLV--NAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKV 671
            +TN  IK++++ DS+ +   ++L+  NA+YFKGAWS KFD +LT   DF++     +KV
Sbjct: 144 VHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTKSYDFHLLDGTMVKV 203

Query: 672 PMM--YKRGDYKY 704
           P M  YK+   +Y
Sbjct: 204 PFMTNYKKQYLEY 216


>At2g26390.1 68415.m03167 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 389

 Score = 91.1 bits (216), Expect = 7e-19
 Identities = 53/186 (28%), Positives = 88/186 (47%), Gaps = 6/186 (3%)
 Frame = +3

Query: 156 IIQGKSVVLSAFSVLPPLAQLALASDGETHEELLKAIGFPDDDAIRTEFASKSRDLRSIK 335
           +  G +VV S  S+   L+ +A  S+  T EE+L  +  P  D +    A  +       
Sbjct: 26  VANGSNVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERS 85

Query: 336 GVELKMANKVYVHDGGKLDENFAVVSRDVFNSDVQNIDFSKNTVAA-KSINDWVEENTNN 512
            + L  A+ V++     L  +F  +  + + +    +DF+   V     +N W + +TN 
Sbjct: 86  DLCLSTAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFATKPVEVIDEVNIWADVHTNG 145

Query: 513 RIKDLVNPDSLSSA-----TAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKVPM 677
            IK +++ D   +      +  +L NA+YFK AWS KFD +LT D DF++    T+KVP 
Sbjct: 146 LIKQILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDNDFHLLDGNTVKVPF 205

Query: 678 MYKRGD 695
           M    D
Sbjct: 206 MMSYKD 211


>At1g47710.1 68414.m05302 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 391

 Score = 85.4 bits (202), Expect = 4e-17
 Identities = 50/181 (27%), Positives = 86/181 (47%), Gaps = 6/181 (3%)
 Frame = +3

Query: 156 IIQGKSVVLSAFSVLPPLAQLALASDGETHEELLKAIGFPDDDAIRTEFASKS-----RD 320
           + Q  +V+ S  S+   L+ +A  S G T +++L  + F   D + + F+S+       D
Sbjct: 25  VSQNSNVIFSPASINVVLSIIAAGSAGATKDQILSFLKFSSTDQLNS-FSSEIVSAVLAD 83

Query: 321 LRSIKGVELKMANKVYVHDGGKLDENFAVVSRDVFNSDVQNIDF-SKNTVAAKSINDWVE 497
             +  G +L +AN  ++        +F  +  D + +     DF SK       +N W E
Sbjct: 84  GSANGGPKLSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQSKAVEVIAEVNSWAE 143

Query: 498 ENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKVPM 677
           + TN  I +++   S  S T  +  NA+YFKG W+ KFDE LT + +F++     +  P 
Sbjct: 144 KETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQEGEFHLLDGNKVTAPF 203

Query: 678 M 680
           M
Sbjct: 204 M 204


>At2g25240.1 68415.m03020 serpin, putative / serine protease
           inhibitor, putative similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 324

 Score = 82.6 bits (195), Expect = 3e-16
 Identities = 41/117 (35%), Positives = 67/117 (57%), Gaps = 3/117 (2%)
 Frame = +3

Query: 339 VELKMANKVYVHDGGKLDENFAVVSRDVFNSDVQNIDF-SKNTVAAKSINDWVEENTNNR 515
           + L +AN V++     L  +F  +  + + +    +DF SK +     +N W E +TN  
Sbjct: 25  LRLSIANGVWIDKFFSLKLSFKDLLENSYKATCSQVDFASKPSEVIDEVNTWAEVHTNGL 84

Query: 516 IKDLVNPDSLSS--ATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKVPMM 680
           IK +++ DS+ +  ++  VL NA+YFKGAWSSKFD  +T   DF++    ++KVP M
Sbjct: 85  IKQILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKNDFHLLDGTSVKVPFM 141


>At1g64030.1 68414.m07252 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311, serpin [Triticum
           aestivum] GI:871551; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 385

 Score = 82.2 bits (194), Expect = 3e-16
 Identities = 50/176 (28%), Positives = 84/176 (47%), Gaps = 6/176 (3%)
 Frame = +3

Query: 171 SVVLSAFSVLPPLAQLALASDGE-THEELLKAIGFPDDDAIRTEFASKSR----DLRSIK 335
           +V+ S  S+   +   A    G+    ++L  +     D ++T F   +     D  +  
Sbjct: 30  NVIFSPASINSAITMHAAGPGGDLVSGQILSFLRSSSIDELKTVFRELASVVYADRSATG 89

Query: 336 GVELKMANKVYVHDGGKLDENFAVVSRDVFNSDVQNIDF-SKNTVAAKSINDWVEENTNN 512
           G ++  AN +++      D  F  +  + F +    +DF S+     K +N WVE +TNN
Sbjct: 90  GPKITAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWVEHHTNN 149

Query: 513 RIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKVPMM 680
            IKDL+   S++S T  +  NA+ FKGAW   F++  T D DFY+    ++ VP M
Sbjct: 150 LIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYLVNGTSVSVPFM 205


>At1g62170.1 68414.m07013 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           GI:9937311 from [Cucurbita maxima]; contains Pfam
           profile PF00079: Serpin (serine protease inhibitor)
          Length = 433

 Score = 77.4 bits (182), Expect = 1e-14
 Identities = 54/184 (29%), Positives = 92/184 (50%), Gaps = 9/184 (4%)
 Frame = +3

Query: 156 IIQGKSVVLSAFSVLPPLAQLALASDGETHEELLKAIGFPDDDAIRTEFASKSRDLRSIK 335
           + +  + V S  S+   L  +A +S GE  EEL   I      +   E  +  R++ S+ 
Sbjct: 86  VAKNSNFVFSPASINAALTMVAASSGGEQGEELRSFILSFLKSSSTDELNAIFREIASVV 145

Query: 336 GVE--LKMANKVYVHDGGKLDENFAV--VSRDVF----NSDVQNIDF-SKNTVAAKSIND 488
            V+   K   K+ V +G  +D++ +V  +S+D+F    ++    +DF SK       +N 
Sbjct: 146 LVDGSKKGGPKIAVVNGMWMDQSLSVNPLSKDLFKNFFSAAFAQVDFRSKAEEVRTEVNA 205

Query: 489 WVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIK 668
           W   +TN  IKDL+   S++S T  V  +A+YFKG W  K+ + +T  + FY+    ++ 
Sbjct: 206 WASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKSMTKCKPFYLLNGTSVS 265

Query: 669 VPMM 680
           VP M
Sbjct: 266 VPFM 269


>At2g14540.1 68415.m01628 serpin family protein / serine protease
           inhibitor family protein similar to phloem serpin-1
           [Cucurbita maxima] GI:9937311; contains Pfam profile
           PF00079: Serpin (serine protease inhibitor)
          Length = 407

 Score = 75.4 bits (177), Expect = 4e-14
 Identities = 47/181 (25%), Positives = 86/181 (47%), Gaps = 6/181 (3%)
 Frame = +3

Query: 156 IIQGKSVVLSAFSVLPPLAQLALASDGETHEEL----LKAIGFPDDDAIRTEFASKS-RD 320
           + +  + V S  S+   L   A  +D +T        LK+    + +AI  E AS   +D
Sbjct: 53  VAKNSNCVFSPASINAVLTVTAANTDNKTLRSFILSFLKSSSTEETNAIFHELASVVFKD 112

Query: 321 LRSIKGVELKMANKVYVHDGGKLDENFAVVSRDVFNSDVQNIDFSKNTVAAK-SINDWVE 497
                G ++   N V++      + ++  +  + F +    +DF       +  +N W  
Sbjct: 113 GSETGGPKIAAVNGVWMEQSLSCNPDWEDLFLNFFKASFAKVDFRHKAEEVRLDVNTWAS 172

Query: 498 ENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKVPM 677
            +TN+ IK+++   S++S T  +  NA+YFKGAW   FD+ +T D+ F++   K++ VP 
Sbjct: 173 RHTNDLIKEILPRGSVTSLTNWIYGNALYFKGAWEKAFDKSMTRDKPFHLLNGKSVSVPF 232

Query: 678 M 680
           M
Sbjct: 233 M 233


>At2g35580.1 68415.m04357 serpin family protein / serine protease
           inhibitor family protein similar to protein zx [Hordeum
           vulgare subsp. vulgare] GI:19071, serpin [Triticum
           aestivum] GI:1885350; contains Pfam profile PF00079:
           Serpin (serine protease inhibitor)
          Length = 374

 Score = 68.5 bits (160), Expect = 5e-12
 Identities = 46/167 (27%), Positives = 82/167 (49%), Gaps = 9/167 (5%)
 Frame = +3

Query: 207 LAQLALASDGETHE-----ELLKAIGFPDDDAIRTEFASKS-RDLRSIKGVELKMANKVY 368
           L+ +A +S G+T        LL+A       A+ +E  +    D  +  G  +  AN ++
Sbjct: 40  LSIIAASSPGDTDTADKIVSLLQASSTDKLHAVSSEIVTTVLADSTASGGPTISAANGLW 99

Query: 369 VHDGGKLDENFAVVSRDVFNSDVQNIDF-SKNTVAAKSINDWVEENTNNRIKDLV--NPD 539
           +     ++ +F  +  + + +    +DF +K     + +N WVE+ TN  I +L+  NP 
Sbjct: 100 IEKTLNVEPSFKDLLLNSYKAAFNRVDFRTKADEVNREVNSWVEKQTNGLITNLLPSNPK 159

Query: 540 SLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDKTIKVPMM 680
           S +  T  +  NA++F G W S+FD  LT D DF++     ++VP M
Sbjct: 160 S-APLTDHIFANALFFNGRWDSQFDPSLTKDSDFHLLDGTKVRVPFM 205


>At1g51330.1 68414.m05772 serpin-related / serine protease
           inhibitor-related similar to serpin [Hordeum vulgare
           subsp. vulgare] CAA64599.1  GI:1197577
          Length = 193

 Score = 64.5 bits (150), Expect = 7e-11
 Identities = 29/75 (38%), Positives = 46/75 (61%)
 Frame = +3

Query: 480 INDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSKDK 659
           +N W   +TN  IK+L+ P S+++ T  +  NA+YFKGAW +KF + +T  + F++   K
Sbjct: 39  VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHKPFHLVNGK 98

Query: 660 TIKVPMMYKRGDYKY 704
            + VP M K  + KY
Sbjct: 99  QVLVPFM-KSYERKY 112


>At1g63280.1 68414.m07154 serpin-related / serine protease
           inhibitor-related similar to protein zx [Hordeum vulgare
           subsp. vulgare] GI:19071, serpin [Triticum aestivum]
           GI:1885346
          Length = 120

 Score = 63.7 bits (148), Expect = 1e-10
 Identities = 29/69 (42%), Positives = 42/69 (60%)
 Frame = +3

Query: 474 KSINDWVEENTNNRIKDLVNPDSLSSATAAVLVNAIYFKGAWSSKFDERLTSDRDFYVSK 653
           + +N W  ++TN  I DL+   S+ S T  V  NA+YFKGAW +KFD+  T D +F+  K
Sbjct: 14  QELNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNEFHQGK 73

Query: 654 DKTIKVPMM 680
           +  + VP M
Sbjct: 74  E--VHVPFM 80


>At3g15920.1 68416.m02013 phox (PX) domain-containing protein weak
           similarity to myosin heavy chain [Rana catesbeiana]
           GI:4249699; contains Pfam profile PF00787: PX domain
          Length = 755

 Score = 28.3 bits (60), Expect = 6.0
 Identities = 27/104 (25%), Positives = 44/104 (42%), Gaps = 5/104 (4%)
 Frame = +3

Query: 372 HDGGKLDENFAVVSRDVFNSDVQNIDFSKNTVAAKSINDWVEENTNNRIKDLVN----PD 539
           HD    DE     +     ++++N+ FS+N+ A  ++ DW E  T   + D  +     +
Sbjct: 262 HDNDSADETSNASTMKHDEANLKNL-FSRNSTAVDNVTDWHELITEYGLLDQSSFQEKVE 320

Query: 540 SLSSATAAVLVNAIYFKGAWSSKFDERLT-SDRDFYVSKDKTIK 668
            LSS         +   G  S    +RL  SDR F     ++IK
Sbjct: 321 RLSSTNGDAATGTVTRGGISSGVGIQRLDGSDRKFQELTIESIK 364


>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
           similar to PRLI-interacting factor L [Arabidopsis
           thaliana] GI:11139268; contains Pfam profile PF02492:
           Cobalamin synthesis protein/P47K
          Length = 444

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 10/29 (34%), Positives = 14/29 (48%)
 Frame = +1

Query: 4   HSRDAGHKHEDNHLFVYYRHRGNGSRHKS 90
           H  D  H H+ NH   ++ H G+   H S
Sbjct: 312 HDHDHDHHHDHNHDHDHHHHDGHDHHHHS 340


>At1g72410.1 68414.m08374 COP1-interacting protein-related similar
           to COP1-Interacting ProteinI 7 (CIP7) [Arabidopsis
           thaliana] GI:3327870
          Length = 1163

 Score = 27.9 bits (59), Expect = 7.9
 Identities = 11/33 (33%), Positives = 18/33 (54%)
 Frame = -2

Query: 175 TLXPWIIFHYFGKHSGCEVIVSIFEHIREICDG 77
           T+  +++F    KHS CE+ VS  E   ++  G
Sbjct: 5   TVLDYVVFELSPKHSKCELFVSSNEQTEKLASG 37


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,231,179
Number of Sequences: 28952
Number of extensions: 302131
Number of successful extensions: 960
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 933
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 956
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1721869952
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -