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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0357
         (379 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein ...    29   1.0  
At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong simil...    28   2.4  
At5g44830.1 68418.m05494 glycoside hydrolase family 28 protein /...    27   5.5  
At5g28480.1 68418.m03462 hypothetical protein                          27   5.5  
At2g12100.1 68415.m01300 Ulp1 protease family protein contains P...    27   5.5  
At1g56230.1 68414.m06464 expressed protein                             27   5.5  
At1g16520.1 68414.m01977 expressed protein                             26   7.2  
At5g45920.1 68418.m05647 GDSL-motif lipase/hydrolase family prot...    26   9.6  
At3g51470.1 68416.m05637 protein phosphatase 2C, putative / PP2C...    26   9.6  
At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identica...    26   9.6  

>At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein
           contains Pfam domain PF02141: DENN (AEX-3) domain; this
           cDNA may contain an anomalously spliced intron...will
           require further examination.
          Length = 765

 Score = 29.1 bits (62), Expect = 1.0
 Identities = 21/80 (26%), Positives = 39/80 (48%), Gaps = 5/80 (6%)
 Frame = +1

Query: 79  WQDFQDFT-LRRMFKKYSQLGVAAL-PDDKFQALMRTVSGMESNYATAKICSY---KNES 243
           W  FQD   L+   + +  + V  L P+   QAL R     +S  ++ ++ S    +N S
Sbjct: 93  WAKFQDHKPLKDPSRLFESVVVVGLHPNCDIQALERQYIARKSEGSSGRLRSALVSQNHS 152

Query: 244 KCDLSLEPEITEIFSTSQDP 303
           + + SLEP++  ++   + P
Sbjct: 153 RVEPSLEPQVLLVYPPDKQP 172


>At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong
           similarity to acyl-CoA oxidase [Arabidopsis thaliana]
           GI:3044214
          Length = 664

 Score = 27.9 bits (59), Expect = 2.4
 Identities = 15/37 (40%), Positives = 18/37 (48%)
 Frame = -1

Query: 319 HASTPQGLGSWRIFQLSQAPKIDRIYFHSCTSRSSQW 209
           H S  QGL +   F     PK D+   HS T  SS+W
Sbjct: 142 HGSNVQGLETTATFD----PKTDQFIIHSPTQTSSKW 174


>At5g44830.1 68418.m05494 glycoside hydrolase family 28 protein /
          polygalacturonase (pectinase) family protein similar to
          polygalacturonase [Lycopersicon esculentum] GI:4325090;
          contains PF00295: Glycosyl hydrolases family 28
          (polygalacturonases)
          Length = 332

 Score = 26.6 bits (56), Expect = 5.5
 Identities = 8/17 (47%), Positives = 13/17 (76%)
 Frame = +1

Query: 46 KAAWEETKMYGWQDFQD 96
          KAAW E+K++ W  F++
Sbjct: 49 KAAWSESKLFRWVSFKE 65


>At5g28480.1 68418.m03462 hypothetical protein
          Length = 1230

 Score = 26.6 bits (56), Expect = 5.5
 Identities = 17/83 (20%), Positives = 37/83 (44%)
 Frame = +1

Query: 94   DFTLRRMFKKYSQLGVAALPDDKFQALMRTVSGMESNYATAKICSYKNESKCDLSLEPEI 273
            + + RR +    +  V      K +A++  V+  E      ++ ++ +++K D   +P++
Sbjct: 909  NISTRRAYSTGKEDDVVEAGVSKTEAIVAPVNEKEKKSVRRRV-TFSDDTKNDDKKDPDV 967

Query: 274  TEIFSTSQDPEELKHAWVEWHNP 342
            T +      PE L  A    +NP
Sbjct: 968  TFVEEREAGPEVLVKAGDSLYNP 990


>At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam
            profile PF02902: Ulp1 protease family, C-terminal
            catalytic domain; similar to At5g28270, At2g05450,
            At1g45090, At2g16180, At2g06750
          Length = 1224

 Score = 26.6 bits (56), Expect = 5.5
 Identities = 17/83 (20%), Positives = 37/83 (44%)
 Frame = +1

Query: 94   DFTLRRMFKKYSQLGVAALPDDKFQALMRTVSGMESNYATAKICSYKNESKCDLSLEPEI 273
            + + RR +    +  V      K +A++  V+  E      ++ ++ +++K D   +P++
Sbjct: 879  NISTRRAYSVRKEDDVVEAGVSKTEAIVAPVNEKEKKSVRRRV-TFSDDTKNDDKKDPDV 937

Query: 274  TEIFSTSQDPEELKHAWVEWHNP 342
            T +      PE L  A    +NP
Sbjct: 938  TFVEEREAGPEVLVKAGDSLYNP 960


>At1g56230.1 68414.m06464 expressed protein
          Length = 752

 Score = 26.6 bits (56), Expect = 5.5
 Identities = 15/45 (33%), Positives = 23/45 (51%)
 Frame = +1

Query: 211 TAKICSYKNESKCDLSLEPEITEIFSTSQDPEELKHAWVEWHNPA 345
           T +   YK E + D   E  +T + ST +DP E   A ++W + A
Sbjct: 596 TGRRMQYKGEDEEDD--EGFVTVVRSTEEDPTEKATALIDWKHQA 638


>At1g16520.1 68414.m01977 expressed protein
          Length = 325

 Score = 26.2 bits (55), Expect = 7.2
 Identities = 16/50 (32%), Positives = 22/50 (44%)
 Frame = -1

Query: 175 SKLGICRQAEPQRPADCIS*TFYAT*NPGSPATRTSWSPPRLLSLDARAP 26
           S  G      P+R +  +S T      P S  +  + SPPR  +L AR P
Sbjct: 208 SPRGYSAAGSPKRTSGAVSPTKATLWYPSSQQSSAANSPPRNRTLPARTP 257


>At5g45920.1 68418.m05647 GDSL-motif lipase/hydrolase family protein
           contains similarity to SP|P41734 Isoamyl
           acetate-hydrolyzing esterase (EC 3.1.-.-) {Saccharomyces
           cerevisiae}; contains Pfam profile PF00657:
           Lipase/Acylhydrolase with GDSL-like motif
          Length = 205

 Score = 25.8 bits (54), Expect = 9.6
 Identities = 9/27 (33%), Positives = 17/27 (62%)
 Frame = +3

Query: 156 RQIPSFDENCVWNGIELCHCEDLLVQE 236
           +QIP++   C+W+G+ L    + +V E
Sbjct: 141 QQIPNWQTECLWDGLHLSRVGNKVVFE 167


>At3g51470.1 68416.m05637 protein phosphatase 2C, putative / PP2C,
           putative protein phosphatase-2C, Mesembryanthemum
           crystallinum, EMBL:AF075580
          Length = 361

 Score = 25.8 bits (54), Expect = 9.6
 Identities = 11/20 (55%), Positives = 13/20 (65%)
 Frame = +1

Query: 241 SKCDLSLEPEITEIFSTSQD 300
           S C LS EPE+ EI  T +D
Sbjct: 248 SLCPLSCEPELEEIVLTEED 267


>At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identical
           to male sterility protein 2 (MS2) SP:Q08891 (Arabidopsis
           thaliana)
          Length = 616

 Score = 25.8 bits (54), Expect = 9.6
 Identities = 11/32 (34%), Positives = 14/32 (43%)
 Frame = +1

Query: 7   KSIQTHLELSRQEKAAWEETKMYGWQDFQDFT 102
           K  Q   E  + +    E  + YGWQD   FT
Sbjct: 329 KGTQNQDEAQKMKDLGLERARSYGWQDTYVFT 360


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.313    0.126    0.379 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,954,673
Number of Sequences: 28952
Number of extensions: 175597
Number of successful extensions: 428
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 422
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 428
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 517767328
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)

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