BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0357 (379 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein ... 29 1.0 At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong simil... 28 2.4 At5g44830.1 68418.m05494 glycoside hydrolase family 28 protein /... 27 5.5 At5g28480.1 68418.m03462 hypothetical protein 27 5.5 At2g12100.1 68415.m01300 Ulp1 protease family protein contains P... 27 5.5 At1g56230.1 68414.m06464 expressed protein 27 5.5 At1g16520.1 68414.m01977 expressed protein 26 7.2 At5g45920.1 68418.m05647 GDSL-motif lipase/hydrolase family prot... 26 9.6 At3g51470.1 68416.m05637 protein phosphatase 2C, putative / PP2C... 26 9.6 At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identica... 26 9.6 >At5g35560.1 68418.m04231 DENN (AEX-3) domain-containing protein contains Pfam domain PF02141: DENN (AEX-3) domain; this cDNA may contain an anomalously spliced intron...will require further examination. Length = 765 Score = 29.1 bits (62), Expect = 1.0 Identities = 21/80 (26%), Positives = 39/80 (48%), Gaps = 5/80 (6%) Frame = +1 Query: 79 WQDFQDFT-LRRMFKKYSQLGVAAL-PDDKFQALMRTVSGMESNYATAKICSY---KNES 243 W FQD L+ + + + V L P+ QAL R +S ++ ++ S +N S Sbjct: 93 WAKFQDHKPLKDPSRLFESVVVVGLHPNCDIQALERQYIARKSEGSSGRLRSALVSQNHS 152 Query: 244 KCDLSLEPEITEIFSTSQDP 303 + + SLEP++ ++ + P Sbjct: 153 RVEPSLEPQVLLVYPPDKQP 172 >At2g35690.1 68415.m04377 acyl-CoA oxidase, putative strong similarity to acyl-CoA oxidase [Arabidopsis thaliana] GI:3044214 Length = 664 Score = 27.9 bits (59), Expect = 2.4 Identities = 15/37 (40%), Positives = 18/37 (48%) Frame = -1 Query: 319 HASTPQGLGSWRIFQLSQAPKIDRIYFHSCTSRSSQW 209 H S QGL + F PK D+ HS T SS+W Sbjct: 142 HGSNVQGLETTATFD----PKTDQFIIHSPTQTSSKW 174 >At5g44830.1 68418.m05494 glycoside hydrolase family 28 protein / polygalacturonase (pectinase) family protein similar to polygalacturonase [Lycopersicon esculentum] GI:4325090; contains PF00295: Glycosyl hydrolases family 28 (polygalacturonases) Length = 332 Score = 26.6 bits (56), Expect = 5.5 Identities = 8/17 (47%), Positives = 13/17 (76%) Frame = +1 Query: 46 KAAWEETKMYGWQDFQD 96 KAAW E+K++ W F++ Sbjct: 49 KAAWSESKLFRWVSFKE 65 >At5g28480.1 68418.m03462 hypothetical protein Length = 1230 Score = 26.6 bits (56), Expect = 5.5 Identities = 17/83 (20%), Positives = 37/83 (44%) Frame = +1 Query: 94 DFTLRRMFKKYSQLGVAALPDDKFQALMRTVSGMESNYATAKICSYKNESKCDLSLEPEI 273 + + RR + + V K +A++ V+ E ++ ++ +++K D +P++ Sbjct: 909 NISTRRAYSTGKEDDVVEAGVSKTEAIVAPVNEKEKKSVRRRV-TFSDDTKNDDKKDPDV 967 Query: 274 TEIFSTSQDPEELKHAWVEWHNP 342 T + PE L A +NP Sbjct: 968 TFVEEREAGPEVLVKAGDSLYNP 990 >At2g12100.1 68415.m01300 Ulp1 protease family protein contains Pfam profile PF02902: Ulp1 protease family, C-terminal catalytic domain; similar to At5g28270, At2g05450, At1g45090, At2g16180, At2g06750 Length = 1224 Score = 26.6 bits (56), Expect = 5.5 Identities = 17/83 (20%), Positives = 37/83 (44%) Frame = +1 Query: 94 DFTLRRMFKKYSQLGVAALPDDKFQALMRTVSGMESNYATAKICSYKNESKCDLSLEPEI 273 + + RR + + V K +A++ V+ E ++ ++ +++K D +P++ Sbjct: 879 NISTRRAYSVRKEDDVVEAGVSKTEAIVAPVNEKEKKSVRRRV-TFSDDTKNDDKKDPDV 937 Query: 274 TEIFSTSQDPEELKHAWVEWHNP 342 T + PE L A +NP Sbjct: 938 TFVEEREAGPEVLVKAGDSLYNP 960 >At1g56230.1 68414.m06464 expressed protein Length = 752 Score = 26.6 bits (56), Expect = 5.5 Identities = 15/45 (33%), Positives = 23/45 (51%) Frame = +1 Query: 211 TAKICSYKNESKCDLSLEPEITEIFSTSQDPEELKHAWVEWHNPA 345 T + YK E + D E +T + ST +DP E A ++W + A Sbjct: 596 TGRRMQYKGEDEEDD--EGFVTVVRSTEEDPTEKATALIDWKHQA 638 >At1g16520.1 68414.m01977 expressed protein Length = 325 Score = 26.2 bits (55), Expect = 7.2 Identities = 16/50 (32%), Positives = 22/50 (44%) Frame = -1 Query: 175 SKLGICRQAEPQRPADCIS*TFYAT*NPGSPATRTSWSPPRLLSLDARAP 26 S G P+R + +S T P S + + SPPR +L AR P Sbjct: 208 SPRGYSAAGSPKRTSGAVSPTKATLWYPSSQQSSAANSPPRNRTLPARTP 257 >At5g45920.1 68418.m05647 GDSL-motif lipase/hydrolase family protein contains similarity to SP|P41734 Isoamyl acetate-hydrolyzing esterase (EC 3.1.-.-) {Saccharomyces cerevisiae}; contains Pfam profile PF00657: Lipase/Acylhydrolase with GDSL-like motif Length = 205 Score = 25.8 bits (54), Expect = 9.6 Identities = 9/27 (33%), Positives = 17/27 (62%) Frame = +3 Query: 156 RQIPSFDENCVWNGIELCHCEDLLVQE 236 +QIP++ C+W+G+ L + +V E Sbjct: 141 QQIPNWQTECLWDGLHLSRVGNKVVFE 167 >At3g51470.1 68416.m05637 protein phosphatase 2C, putative / PP2C, putative protein phosphatase-2C, Mesembryanthemum crystallinum, EMBL:AF075580 Length = 361 Score = 25.8 bits (54), Expect = 9.6 Identities = 11/20 (55%), Positives = 13/20 (65%) Frame = +1 Query: 241 SKCDLSLEPEITEIFSTSQD 300 S C LS EPE+ EI T +D Sbjct: 248 SLCPLSCEPELEEIVLTEED 267 >At3g11980.1 68416.m01482 male sterility protein 2 (MS2) identical to male sterility protein 2 (MS2) SP:Q08891 (Arabidopsis thaliana) Length = 616 Score = 25.8 bits (54), Expect = 9.6 Identities = 11/32 (34%), Positives = 14/32 (43%) Frame = +1 Query: 7 KSIQTHLELSRQEKAAWEETKMYGWQDFQDFT 102 K Q E + + E + YGWQD FT Sbjct: 329 KGTQNQDEAQKMKDLGLERARSYGWQDTYVFT 360 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.313 0.126 0.379 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 8,954,673 Number of Sequences: 28952 Number of extensions: 175597 Number of successful extensions: 428 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 422 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 428 length of database: 12,070,560 effective HSP length: 73 effective length of database: 9,957,064 effective search space used: 517767328 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.2 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 42 (22.0 bits)
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