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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0347
         (759 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g64440.1 68418.m08095 amidase family protein low similarity t...    35   0.067
At3g25660.1 68416.m03194 glutamyl-tRNA(Gln) amidotransferase, pu...    34   0.12 
At4g09430.1 68417.m01553 disease resistance protein (TIR-NBS-LRR...    28   5.9  

>At5g64440.1 68418.m08095 amidase family protein low similarity to
           enantiomerase-selective amidase [Rhodococcus sp.]
           GI:152052; contains Pfam profile PF01425: Amidase
          Length = 607

 Score = 34.7 bits (76), Expect = 0.067
 Identities = 14/44 (31%), Positives = 24/44 (54%)
 Frame = -3

Query: 595 NALGLPVTACPIEMSKKGLPVGIQIAANRYKDHLTVAVANEFEK 464
           N LG P  + P+   K+GLP+G+QI    + +   + +A   E+
Sbjct: 546 NLLGFPAISVPVGYDKEGLPIGLQIMGRPWAEATVLGLAAAVEE 589


>At3g25660.1 68416.m03194 glutamyl-tRNA(Gln) amidotransferase,
           putative similar to SP|O06491 Glutamyl-tRNA(Gln)
           amidotransferase subunit A (EC 6.3.5.-) {Bacillus
           subtilis}; contains Pfam profile PF01425: Amidase
          Length = 537

 Score = 33.9 bits (74), Expect = 0.12
 Identities = 29/112 (25%), Positives = 47/112 (41%), Gaps = 10/112 (8%)
 Frame = -3

Query: 757 YYKNLLTKFEDIKTEFETILSDDAVLVFPTFPHPAHLHYRVYYK-----FLNCGYLTMFN 593
           YYK        I+ +F+  L  + +L+ P  P  A   Y++  K      +  G +   N
Sbjct: 417 YYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAA---YKIGEKKDDPLAMYAGDIMTVN 473

Query: 592 A--LGLPVTACP---IEMSKKGLPVGIQIAANRYKDHLTVAVANEFEKAFGG 452
               GLP    P   +E    GLPVG+Q+    + +   + V + FE+   G
Sbjct: 474 VNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKVGHIFEQTLKG 525


>At4g09430.1 68417.m01553 disease resistance protein (TIR-NBS-LRR
            class), putative domain signature TIR-NBS-LRR exists,
            suggestive of a disease resistance protein.
          Length = 1039

 Score = 28.3 bits (60), Expect = 5.9
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +1

Query: 628  YSTLGSASGRDVEKSETPKLHRQIKLSQ 711
            YS     SGR++ K+  PKL  Q+KL Q
Sbjct: 982  YSQNPKTSGREIPKTALPKLGLQLKLPQ 1009


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,633,236
Number of Sequences: 28952
Number of extensions: 324854
Number of successful extensions: 600
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 600
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1692519896
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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