Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0336
(746 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II comp... 352 4e-98
SPAC4G9.08c |rpc2||DNA-directed RNA polymerase III complex subun... 241 6e-65
SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subuni... 177 2e-45
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 6.6
SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subuni... 26 6.6
SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase |Schi... 25 8.7
>SPAC23G3.01 |rpb2|SPAC521.06|DNA-directed RNA polymerase II complex
subunit Rpb2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1210
Score = 352 bits (865), Expect = 4e-98
Identities = 159/226 (70%), Positives = 191/226 (84%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFNDA 182
G+ YR EDMPF+ +GI PDIIINPHAIPSRMT+ HL+EC KVS+ G GDATPF D
Sbjct: 980 GMTYRHEDMPFSAQGIVPDIIINPHAIPSRMTVAHLVECQLSKVSALSGFEGDATPFTD- 1038
Query: 183 VNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARG 362
V V+ +S LL+ +G+ RG EVMY+GHTGRK+ AQVFLGPTYYQRLKH+VDDKIH+RARG
Sbjct: 1039 VTVEAVSKLLRSHGFQSRGFEVMYHGHTGRKLVAQVFLGPTYYQRLKHLVDDKIHARARG 1098
Query: 363 PVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNFCGLI 542
PVQIL RQP+EGR+RDGGLRFGEMERDCQI+HG + LRERLF+ SD YR+ VC+ CGLI
Sbjct: 1099 PVQILTRQPVEGRSRDGGLRFGEMERDCQISHGCSSVLRERLFDCSDAYRVIVCDICGLI 1158
Query: 543 AIANLRNNTFECKGCKNKTQISQIRLPYAAKLLFQELMSMNIAPRL 680
AIA+ + +++EC+ C+N+T+ SQ+ LPYAAKLLFQELMSMNIAPRL
Sbjct: 1159 AIASYKKDSYECRSCQNRTRFSQVYLPYAAKLLFQELMSMNIAPRL 1204
>SPAC4G9.08c |rpc2||DNA-directed RNA polymerase III complex subunit
Rpc2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1165
Score = 241 bits (591), Expect = 6e-65
Identities = 115/226 (50%), Positives = 153/226 (67%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFNDA 182
G+ +QEDMPF +GI PDII+NPH PSRMT+G +IE + GKV +G + T F
Sbjct: 939 GVIVQQEDMPFNDQGICPDIIMNPHGFPSRMTVGKMIELLSGKVGVLRGTLEYGTCFG-G 997
Query: 183 VNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARG 362
V+ S +L ++GY+ G +++ +G TG + A +F+GP YYQ+LKHMV DK+H+RARG
Sbjct: 998 TKVEDASRILVEHGYNYSGKDMLTSGITGETLEAYIFMGPIYYQKLKHMVMDKMHARARG 1057
Query: 363 PVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNFCGLI 542
P +L RQP EGR+RDGGLR GEMERDC IA+GA+Q L ERL SD + VC CGL+
Sbjct: 1058 PRAVLTRQPTEGRSRDGGLRLGEMERDCLIAYGASQLLLERLMISSDACDVDVCGQCGLL 1117
Query: 543 AIANLRNNTFECKGCKNKTQISQIRLPYAAKLLFQELMSMNIAPRL 680
C C++ ++ ++ +PYAAKLLFQEL+SMNI PRL
Sbjct: 1118 GYKGW------CNSCQSTREVVKMTIPYAAKLLFQELLSMNIVPRL 1157
>SPBP23A10.07 |rpa2||DNA-directed RNA polymerase I complex subunit
Rpa2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1227
Score = 177 bits (430), Expect = 2e-45
Identities = 86/176 (48%), Positives = 108/176 (61%), Gaps = 3/176 (1%)
Frame = +3
Query: 24 DMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPF---NDAVNVQ 194
DMPFT G+ PDIIINPHA PSRMTIG IE + GK + G D+TPF
Sbjct: 973 DMPFTESGMQPDIIINPHAFPSRMTIGMFIESLAGKAGACHGLAQDSTPFIYSEQQTAAD 1032
Query: 195 KISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARGPVQI 374
L GY+ GNE MY+G TG+++ A +++G YYQRL+HMV DK R GP+
Sbjct: 1033 YFGEQLVKAGYNYHGNEPMYSGITGQEMKADIYIGVVYYQRLRHMVSDKFQVRTTGPIHN 1092
Query: 375 LVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNFCGLI 542
L RQP++GR R GG+RFGEMERD I HG + +++RL SD + VC CG I
Sbjct: 1093 LTRQPVKGRKRAGGIRFGEMERDAVIGHGTSFLMQDRLMNCSDYAQSWVCRDCGSI 1148
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 6.6
Identities = 11/38 (28%), Positives = 20/38 (52%)
Frame = +3
Query: 132 VSSNKGEIGDATPFNDAVNVQKISSLLQDYGYHLRGNE 245
+ + E D TPF +N + S +++ +LRG+E
Sbjct: 108 IKRSSSETADFTPFRIPINPDERSEYEKEFRENLRGHE 145
>SPBC12D12.03 |cct1||chaperonin-containing T-complex alpha subunit
Cct1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 25.8 bits (54), Expect = 6.6
Identities = 23/114 (20%), Positives = 47/114 (41%), Gaps = 3/114 (2%)
Frame = +3
Query: 15 RQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNK---GEIGDATPFNDAV 185
R+ + + E I+ + + N + + + I ++++ G V +K +IGD T ND
Sbjct: 6 RESTLFLSGEKISGEDVRNQNVLATT-AIANVVKSSLGPVGLDKMLVDDIGDVTVTNDGA 64
Query: 186 NVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIH 347
+ + + G L + G + V + +R +V +KIH
Sbjct: 65 TILSLLDVEHPAGKVLVELAQQQDKEVGDGTTSVVIIAAELLRRANELVKNKIH 118
>SPAC4D7.02c |||glycerophosphoryl diester phosphodiesterase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 319
Score = 25.4 bits (53), Expect = 8.7
Identities = 8/15 (53%), Positives = 11/15 (73%)
Frame = -3
Query: 633 WLHMVNGFAKFEFCF 589
WLHM+ GF + +F F
Sbjct: 297 WLHMIYGFLRAQFVF 311
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,217,344
Number of Sequences: 5004
Number of extensions: 70447
Number of successful extensions: 148
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 142
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 355273338
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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