Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0336
(746 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_11932| Best HMM Match : RNA_pol_Rpb2_6 (HMM E-Value=0) 447 e-126
SB_29595| Best HMM Match : RNA_pol_Rpb2_6 (HMM E-Value=1.90001e-40) 72 8e-21
SB_8145| Best HMM Match : RNA_pol_Rpb2_6 (HMM E-Value=0) 85 8e-17
SB_28313| Best HMM Match : No HMM Matches (HMM E-Value=.) 62 5e-10
SB_59756| Best HMM Match : cNMP_binding (HMM E-Value=5.7e-21) 32 0.57
SB_45509| Best HMM Match : PX (HMM E-Value=0.063) 30 2.3
SB_57001| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.3
SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16) 29 5.3
SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 5.3
SB_7725| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 9.2
>SB_11932| Best HMM Match : RNA_pol_Rpb2_6 (HMM E-Value=0)
Length = 498
Score = 447 bits (1102), Expect = e-126
Identities = 200/228 (87%), Positives = 217/228 (95%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFNDA 182
GI YRQEDMPFTCEG++PD+IINPHAIPSRMTIGHLIEC+QGKVS+NKGEIGDATPFNDA
Sbjct: 266 GITYRQEDMPFTCEGLSPDVIINPHAIPSRMTIGHLIECLQGKVSANKGEIGDATPFNDA 325
Query: 183 VNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARG 362
VNVQKIS LL DYGYHLRGNEV+Y+G TGRK+N QVFLGPTYYQRLKHMVDDKIHSRARG
Sbjct: 326 VNVQKISQLLNDYGYHLRGNEVLYSGFTGRKLNTQVFLGPTYYQRLKHMVDDKIHSRARG 385
Query: 363 PVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNFCGLI 542
P+QIL RQPMEGR+RDGGLRFGEMERDC IAHG+AQFL ERLFEVSDPY HVCN CGLI
Sbjct: 386 PLQILTRQPMEGRSRDGGLRFGEMERDCMIAHGSAQFLNERLFEVSDPYSCHVCNLCGLI 445
Query: 543 AIANLRNNTFECKGCKNKTQISQIRLPYAAKLLFQELMSMNIAPRLMV 686
AIANLRNNTFEC+GCKNKTQ+S+IR+PYAAKLLFQELMSM+IAPR+MV
Sbjct: 446 AIANLRNNTFECRGCKNKTQVSRIRMPYAAKLLFQELMSMSIAPRMMV 493
>SB_29595| Best HMM Match : RNA_pol_Rpb2_6 (HMM E-Value=1.90001e-40)
Length = 700
Score = 72.1 bits (169), Expect(2) = 8e-21
Identities = 59/198 (29%), Positives = 85/198 (42%), Gaps = 3/198 (1%)
Frame = +3
Query: 102 GHLIECIQGKVSSNKGEIGDATPFN---DAVNVQKISSLLQDYGYHLRGNEVMYNGHTGR 272
G +IE + GK ++ G + DATPF D + LL GY G E +Y+G GR
Sbjct: 483 GMMIESMAGKSAALNGHVYDATPFTFSEDDSAIDYFGKLLVKSGYDYFGTERLYSGIDGR 542
Query: 273 KINAQVFLGPTYYQRLKHMVDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQI 452
+ A +F+G YYQRL+HMV DK ++++ P G A D +
Sbjct: 543 EFEADIFMGVVYYQRLRHMVADKFQVSKYS--SLVIQGPNNG-AIDA------LTHQPVQ 593
Query: 453 AHGAAQFLRERLFEVSDPYRIHVCNFCGLIAIANLRNNTFECKGCKNKTQISQIRLPYAA 632
H Q L + D + L A A + CK C++ I + PY
Sbjct: 594 GHVCTQ-CGSLLSPLLDKPPVE------LAAAAAHSERRWYCKPCESSEHIELLAFPYVF 646
Query: 633 KLLFQELMSMNIAPRLMV 686
+ L EL +MNI L +
Sbjct: 647 RYLVAELAAMNIKTSLEI 664
Score = 46.4 bits (105), Expect(2) = 8e-21
Identities = 19/27 (70%), Positives = 22/27 (81%)
Frame = +3
Query: 21 EDMPFTCEGITPDIIINPHAIPSRMTI 101
E+MPF+ G+TPDII NPH PSRMTI
Sbjct: 429 ENMPFSESGMTPDIIFNPHGFPSRMTI 455
>SB_8145| Best HMM Match : RNA_pol_Rpb2_6 (HMM E-Value=0)
Length = 893
Score = 84.6 bits (200), Expect = 8e-17
Identities = 41/92 (44%), Positives = 56/92 (60%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFNDA 182
G+ QEDMPFT GI PDII+NPH PSRMT+G LIE I GK +G G T F
Sbjct: 336 GLIVNQEDMPFTDLGICPDIIMNPHGFPSRMTVGKLIELIAGKAGVLEGHFGYGTAFG-G 394
Query: 183 VNVQKISSLLQDYGYHLRGNEVMYNGHTGRKI 278
V+ + +L +G++ G + + +G TG++I
Sbjct: 395 DKVEDVCEVLMKHGFNYAGKDYVTSGITGKRI 426
Score = 61.3 bits (142), Expect = 8e-10
Identities = 30/50 (60%), Positives = 35/50 (70%), Gaps = 2/50 (4%)
Frame = +3
Query: 351 RARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGA--AQFLRERLFE 494
R GP +L RQP EGR+RDGGLR GEMERDC IA+GA +RE F+
Sbjct: 484 RFPGPRAVLTRQPTEGRSRDGGLRLGEMERDCLIAYGARLVNVIRELHFD 533
>SB_28313| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 107
Score = 62.1 bits (144), Expect = 5e-10
Identities = 26/51 (50%), Positives = 35/51 (68%)
Frame = +3
Query: 351 RARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSD 503
R GP+ L QP++GR GG+RFGEMERD +AHG + L++RLF S+
Sbjct: 17 RTTGPIDALTHQPVQGRKWQGGIRFGEMERDALLAHGTSFLLQDRLFNCSN 67
>SB_59756| Best HMM Match : cNMP_binding (HMM E-Value=5.7e-21)
Length = 858
Score = 31.9 bits (69), Expect = 0.57
Identities = 29/118 (24%), Positives = 46/118 (38%)
Frame = +3
Query: 177 DAVNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRA 356
+ + V I LL + G + + K+ +VFL Y R H DK++
Sbjct: 608 ETILVHNIKPLLTTVPF-FSGASISFITDIVTKLKFEVFLNGDYICRSGHR-GDKMYFIQ 665
Query: 357 RGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNF 530
+G V IL R+ + G FGE+ C + A + R D Y + +F
Sbjct: 666 KGIVDILTREGALATSLGDGSHFGEI---CLLTKEARRVASVRAATTCDVYSLSATHF 720
>SB_45509| Best HMM Match : PX (HMM E-Value=0.063)
Length = 556
Score = 29.9 bits (64), Expect = 2.3
Identities = 23/75 (30%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Frame = -3
Query: 630 LHMVNGFAKFEFCFCS-LCIQMYCYAN*L*RSGRRNYTRVYGKDLILQINVHVKIGQLRV 454
L+MVN AK+ C C L + M N + G+ Y +++ + +N H KIG
Sbjct: 21 LNMVNAHAKYGQCTCQILSMHMPNMVNAHAKYGQCTY-QIWSMHMPNMVNAHAKIGPCIC 79
Query: 453 QS-DNHVPFHQNVIH 412
Q H+P N++H
Sbjct: 80 QIWSIHIP---NMVH 91
>SB_57001| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1487
Score = 28.7 bits (61), Expect = 5.3
Identities = 13/39 (33%), Positives = 24/39 (61%)
Frame = -3
Query: 513 YGKDLILQINVHVKIGQLRVQSDNHVPFHQNVIHHLLPY 397
Y K+++ Q ++H +GQLR++ V H++ + LPY
Sbjct: 1086 YCKEMLYQGSLHSSLGQLRLELLRIVCSHEHYVTLNLPY 1124
>SB_37014| Best HMM Match : HSP70 (HMM E-Value=5.9e-16)
Length = 212
Score = 28.7 bits (61), Expect = 5.3
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 96 TIGHLIECIQGKVSSNKGEIGDATPFNDAVNVQKISSLLQD 218
T+G +C+Q NKGEIGD + K+ +QD
Sbjct: 69 TVGPCKKCLQD-AEINKGEIGDVLLVGGMTRMPKVQQTVQD 108
>SB_38725| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 766
Score = 28.7 bits (61), Expect = 5.3
Identities = 14/41 (34%), Positives = 20/41 (48%)
Frame = +3
Query: 96 TIGHLIECIQGKVSSNKGEIGDATPFNDAVNVQKISSLLQD 218
T+G +C+Q NKGEIGD + K+ +QD
Sbjct: 124 TVGPCKKCLQD-AEINKGEIGDVLLVGGMTRMPKVQQTVQD 163
>SB_7725| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 277
Score = 27.9 bits (59), Expect = 9.2
Identities = 12/40 (30%), Positives = 20/40 (50%)
Frame = +3
Query: 33 FTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGE 152
FT E I P +++ P + +GHL + S +KG+
Sbjct: 90 FTWEPIPPSLLVKPEDYVKPIRVGHLFRRFRPYGSDSKGD 129
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,577,972
Number of Sequences: 59808
Number of extensions: 506273
Number of successful extensions: 1070
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 978
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1069
length of database: 16,821,457
effective HSP length: 80
effective length of database: 12,036,817
effective search space used: 2022185256
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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