BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0336 (746 letters) Database: bee 438 sequences; 146,343 total letters Searching......................................................done Score E Sequences producing significant alignments: (bits) Value AB208107-1|BAE72139.1| 71|Apis mellifera Broad complex zinc fi... 23 2.3 AY588474-1|AAT94401.1| 104|Apis mellifera defensin 2 protein. 23 3.0 DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chlor... 23 4.0 AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic ac... 23 4.0 DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 22 5.3 DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 22 5.3 DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholi... 22 5.3 EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholi... 22 7.0 EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholi... 22 7.0 EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholi... 22 7.0 EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholi... 22 7.0 EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholi... 22 7.0 EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholi... 22 7.0 DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholi... 22 7.0 DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholi... 22 7.0 AY338499-1|AAR08420.1| 500|Apis mellifera Kruppel-like protein ... 22 7.0 DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholi... 21 9.3 >AB208107-1|BAE72139.1| 71|Apis mellifera Broad complex zinc finger domain-Z2 isoform protein. Length = 71 Score = 23.4 bits (48), Expect = 2.3 Identities = 6/22 (27%), Positives = 15/22 (68%) Frame = -1 Query: 575 FKCIVTQISYSDQAAEITHVYT 510 ++C++ + Y + + +TH+YT Sbjct: 36 YRCVICERVYCSRNSLMTHIYT 57 >AY588474-1|AAT94401.1| 104|Apis mellifera defensin 2 protein. Length = 104 Score = 23.0 bits (47), Expect = 3.0 Identities = 9/16 (56%), Positives = 11/16 (68%) Frame = -1 Query: 311 VVSWSQKNLRINFTAC 264 V+SW K L IN +AC Sbjct: 66 VLSWQSKWLSINHSAC 81 >DQ667188-1|ABG75740.1| 383|Apis mellifera histamine-gated chloride channel protein. Length = 383 Score = 22.6 bits (46), Expect = 4.0 Identities = 8/13 (61%), Positives = 11/13 (84%) Frame = +3 Query: 66 INPHAIPSRMTIG 104 I P AIP+R+T+G Sbjct: 237 IKPEAIPARVTLG 249 >AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic acetylcholine receptoralpha7-1 protein. Length = 555 Score = 22.6 bits (46), Expect = 4.0 Identities = 8/22 (36%), Positives = 13/22 (59%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN + +T LWL W +++W Sbjct: 66 KNQLLITNLWLKLEWNDVNMRW 87 >DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 22.2 bits (45), Expect = 5.3 Identities = 8/22 (36%), Positives = 12/22 (54%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T LW+ +W ++W Sbjct: 71 KNQIMTTNLWVEQSWYDYKLRW 92 >DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine receptor alpha4subunit protein. Length = 569 Score = 22.2 bits (45), Expect = 5.3 Identities = 8/22 (36%), Positives = 12/22 (54%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T LW+ +W ++W Sbjct: 71 KNQIMTTNLWVEQSWYDYKLRW 92 >DQ026032-1|AAY87891.1| 566|Apis mellifera nicotinic acetylcholine receptor alpha3subunit protein. Length = 566 Score = 22.2 bits (45), Expect = 5.3 Identities = 8/22 (36%), Positives = 12/22 (54%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T LW+ +W ++W Sbjct: 67 KNQIMTTNLWVEQSWYDYKLKW 88 >EF127805-1|ABL67942.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 6 protein. Length = 461 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 29 KNQILTTNAWLKLEWVDYNLQW 50 >EF127804-1|ABL67941.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 5 protein. Length = 461 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 29 KNQILTTNAWLKLEWVDYNLQW 50 >EF127803-1|ABL67940.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 4 protein. Length = 461 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 29 KNQILTTNAWLKLEWVDYNLQW 50 >EF127802-1|ABL67939.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 3 protein. Length = 461 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 29 KNQILTTNAWLKLEWVDYNLQW 50 >EF127801-1|ABL67938.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 2 protein. Length = 461 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 29 KNQILTTNAWLKLEWVDYNLQW 50 >EF127800-1|ABL67937.1| 461|Apis mellifera nicotinic acetylcholine receptor subunitalpha 6 transcript variant 1 protein. Length = 461 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 29 KNQILTTNAWLKLEWVDYNLQW 50 >DQ026036-1|AAY87895.1| 529|Apis mellifera nicotinic acetylcholine receptor alpha6subunit protein. Length = 529 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 97 KNQILTTNAWLKLEWVDYNLQW 118 >DQ026035-1|AAY87894.1| 529|Apis mellifera nicotinic acetylcholine receptor alpha6subunit protein. Length = 529 Score = 21.8 bits (44), Expect = 7.0 Identities = 9/22 (40%), Positives = 11/22 (50%) Frame = +2 Query: 194 KNIISVTRLWLPSTW**SHVQW 259 KN I T WL W ++QW Sbjct: 97 KNQILTTNAWLKLEWVDYNLQW 118 >AY338499-1|AAR08420.1| 500|Apis mellifera Kruppel-like protein 1 protein. Length = 500 Score = 21.8 bits (44), Expect = 7.0 Identities = 11/21 (52%), Positives = 11/21 (52%) Frame = +3 Query: 474 LRERLFEVSDPYRIHVCNFCG 536 LR E DPYR CN CG Sbjct: 81 LRSHGKEGEDPYR---CNICG 98 >DQ026038-1|AAY87897.1| 520|Apis mellifera nicotinic acetylcholine receptor beta1subunit protein. Length = 520 Score = 21.4 bits (43), Expect = 9.3 Identities = 20/122 (16%), Positives = 49/122 (40%) Frame = +1 Query: 133 YLLIRERSVMQPHSMML*MCKKYHLCYKIMVTIYVVMKSCTMGIQAVKLMRKFFWDQLTT 312 Y++IR +++ +++L LC + + T+GI + + F L + Sbjct: 226 YIIIRRKTLFYTVNLILPTVLISFLCVLVFYLPAEAGEKVTLGISILLSLVVFLL--LVS 283 Query: 313 NV*STWSMIKFILELEVQYKF**DSLWKVGQEMVDYVLVKWNVIVRLHTELPNFYVNVYL 492 + S++ ++ + + F + +V +++ WN +P ++L Sbjct: 284 KILPPTSLVLPLIAKYLLFTF----IMNTVSILVTVIIINWNFRGPRTHRMPQLIRKIFL 339 Query: 493 KY 498 KY Sbjct: 340 KY 341 Database: bee Posted date: Oct 23, 2007 1:17 PM Number of letters in database: 146,343 Number of sequences in database: 438 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 218,227 Number of Sequences: 438 Number of extensions: 4986 Number of successful extensions: 24 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 24 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 24 length of database: 146,343 effective HSP length: 56 effective length of database: 121,815 effective search space used: 23388480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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