Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0336
(746 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g21710.1 68417.m03144 DNA-directed RNA polymerase II 135 kDa ... 367 e-102
At5g45140.1 68418.m05542 DNA-directed RNA polymerase, putative s... 232 2e-61
At3g23780.1 68416.m02989 DNA-directed RNA polymerase family prot... 185 2e-47
At3g18090.1 68416.m02300 DNA-directed RNA polymerase family prot... 185 2e-47
At1g29940.1 68414.m03658 DNA-directed RNA polymerase family prot... 181 5e-46
At1g72040.1 68414.m08327 deoxynucleoside kinase family contains ... 31 0.61
At1g70020.1 68414.m08058 hypothetical protein 30 1.9
At4g13150.1 68417.m02047 expressed protein 29 4.3
At3g46640.1 68416.m05063 myb family transcription factor contain... 29 4.3
At1g02910.1 68414.m00258 tetratricopeptide repeat (TPR)-containi... 29 4.3
At5g58350.1 68418.m07306 protein kinase family protein contains ... 28 5.7
At3g07150.1 68416.m00852 hypothetical protein 28 5.7
At5g58880.1 68418.m07377 hypothetical protein 28 7.6
At1g73875.1 68414.m08555 endonuclease/exonuclease/phosphatase fa... 28 7.6
At1g15660.1 68414.m01880 expressed protein similar to CENPCA pro... 28 7.6
At1g64810.1 68414.m07348 expressed protein contains Pfam PF05634... 27 10.0
>At4g21710.1 68417.m03144 DNA-directed RNA polymerase II 135 kDa
polypeptide / RNA polymerase II subunit 2 (RPB135) (RPB2)
(RP140) identical to SP|P38420 DNA-directed RNA
polymerase II 135 kDa polypeptide (EC 2.7.7.6) (RNA
polymerase II subunit 2) {Arabidopsis thaliana}
Length = 1188
Score = 367 bits (904), Expect = e-102
Identities = 167/227 (73%), Positives = 189/227 (83%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFNDA 182
G+ Y QEDMP+T EG+TPDII+NPHAIPSRMTIG LIECI GKV+++ G+ GDATPF D
Sbjct: 952 GMTYTQEDMPWTIEGVTPDIIVNPHAIPSRMTIGQLIECIMGKVAAHMGKEGDATPFTD- 1010
Query: 183 VNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHSRARG 362
V V IS L GY +RG E MYNGHTGR + A +FLGPTYYQRLKHMVDDKIHSR RG
Sbjct: 1011 VTVDNISKALHKCGYQMRGFERMYNGHTGRPLTAMIFLGPTYYQRLKHMVDDKIHSRGRG 1070
Query: 363 PVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNFCGLI 542
PVQIL RQP EGR+RDGGLRFGEMERDC IAHGAA FL+ERLF+ SD YR+HVC CGLI
Sbjct: 1071 PVQILTRQPAEGRSRDGGLRFGEMERDCMIAHGAAHFLKERLFDQSDAYRVHVCEVCGLI 1130
Query: 543 AIANLRNNTFECKGCKNKTQISQIRLPYAAKLLFQELMSMNIAPRLM 683
AIANL+ N+FEC+GCKNKT I Q+ +PYA KLLFQELMSM IAPR++
Sbjct: 1131 AIANLKKNSFECRGCKNKTDIVQVYIPYACKLLFQELMSMAIAPRML 1177
>At5g45140.1 68418.m05542 DNA-directed RNA polymerase, putative
similar to SP|P22276 DNA-directed RNA polymerase III 130
kDa polypeptide (EC 2.7.7.6) (RNA polymerase III subunit
2) {Saccharomyces cerevisiae}; contains Pfam profiles
PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567:
RNA polymerase Rpb2 domain 5
Length = 1150
Score = 232 bits (567), Expect = 2e-61
Identities = 108/230 (46%), Positives = 150/230 (65%), Gaps = 4/230 (1%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFND- 179
GI +QED PF+ GI PD+I+NPH PSRMT+G +IE + K + G + F +
Sbjct: 916 GIIIQQEDFPFSELGICPDLIMNPHGFPSRMTVGKMIELLGSKAGVSCGRFHYGSAFGER 975
Query: 180 ---AVNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDKIHS 350
A V+ IS+ L + G+ G +++Y+G +G + A +F+GP YYQ+LKHMV DK+H+
Sbjct: 976 SGHADKVETISATLVEKGFSYSGKDLLYSGISGEPVEAYIFMGPIYYQKLKHMVLDKMHA 1035
Query: 351 RARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNF 530
R GP ++ RQP EG++++GGLR GEMERDC IA+GA+ + ERL SDP+ + VC
Sbjct: 1036 RGSGPRVMMTRQPTEGKSKNGGLRVGEMERDCLIAYGASMLIYERLMISSDPFEVQVCRA 1095
Query: 531 CGLIAIANLRNNTFECKGCKNKTQISQIRLPYAAKLLFQELMSMNIAPRL 680
CGL+ N + C CKN I+ ++LPYA KLLFQEL SMN+ PRL
Sbjct: 1096 CGLLGYYNYKLKKAVCTTCKNGDNIATMKLPYACKLLFQELQSMNVVPRL 1145
>At3g23780.1 68416.m02989 DNA-directed RNA polymerase family protein
similar to SP|P38420 DNA-directed RNA polymerase II 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
2) {Arabidopsis thaliana}; contains Pfam profiles
PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567:
RNA polymerase Rpb2 domain 5
Length = 946
Score = 185 bits (451), Expect = 2e-47
Identities = 101/240 (42%), Positives = 137/240 (57%), Gaps = 18/240 (7%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECI-----------QGKVSSNKG 149
G Q++ PFT +GI PDI+INPHA PSR T G L+E +G ++
Sbjct: 699 GYLEEQQNFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALSKGIACPIQKEGSSAAYTK 758
Query: 150 EIGDATPFNDAVNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHM 329
ATPF+ V +I+ L G+ GNE +YNG +G + + +F+GPT+YQRL HM
Sbjct: 759 LTRHATPFSTP-GVTEITEQLHRAGFSRWGNERVYNGRSGEMMRSMIFMGPTFYQRLVHM 817
Query: 330 VDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPY 509
+DK+ R GPV L RQP+ R R GG++FGEMERDC IAHGA+ L ERLF +SD
Sbjct: 818 SEDKVKFRNTGPVHPLTRQPVADRKRFGGIKFGEMERDCLIAHGASANLHERLFTLSDSS 877
Query: 510 RIHVCNFCGLIAIANLRNNTF-------ECKGCKNKTQISQIRLPYAAKLLFQELMSMNI 668
++H+C C A R + C+ C + + ++ +PY AKLL QEL SM I
Sbjct: 878 QMHICRKCKTYANVIERTPSSGRKIRGPYCRVCVSSDHVVRVYVPYGAKLLCQELFSMGI 937
>At3g18090.1 68416.m02300 DNA-directed RNA polymerase family protein
similar to SP|P38420 DNA-directed RNA polymerase II 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
2) {Arabidopsis thaliana}; contains Pfam profiles
PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567:
RNA polymerase Rpb2 domain 5
Length = 1038
Score = 185 bits (451), Expect = 2e-47
Identities = 102/241 (42%), Positives = 137/241 (56%), Gaps = 19/241 (7%)
Frame = +3
Query: 3 GIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECIQGK-----VSSNKGEIG--- 158
G Q++ PFT +GI PDI+INPHA PSR T G L+E K + +G
Sbjct: 790 GYLEEQQNFPFTIQGIVPDIVINPHAFPSRQTPGQLLEAALSKGIACPIQKKEGSSAAYT 849
Query: 159 ----DATPFNDAVNVQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKH 326
ATPF+ V +I+ L G+ GNE +YNG +G + + +F+GPT+YQRL H
Sbjct: 850 KLTRHATPFSTP-GVTEITEQLHRAGFSRWGNERVYNGRSGEMMRSLIFMGPTFYQRLVH 908
Query: 327 MVDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDP 506
M ++K+ R GPV L RQP+ R R GG+RFGEMERDC IAHGA+ L ERLF +SD
Sbjct: 909 MSENKVKFRNTGPVHPLTRQPVADRKRFGGIRFGEMERDCLIAHGASANLHERLFTLSDS 968
Query: 507 YRIHVCNFCGLIAIANLRNNTF-------ECKGCKNKTQISQIRLPYAAKLLFQELMSMN 665
++H+C C A R + C+ C + + ++ +PY AKLL QEL SM
Sbjct: 969 SQMHICRKCKTYANVIERTPSSGRKIRGPYCRVCASSDHVVRVYVPYGAKLLCQELFSMG 1028
Query: 666 I 668
I
Sbjct: 1029 I 1029
>At1g29940.1 68414.m03658 DNA-directed RNA polymerase family protein
similar to SP|P22138 DNA-directed RNA polymerase I 135
kDa polypeptide (EC 2.7.7.6) (RNA polymerase I subunit 2)
{Saccharomyces cerevisiae}; contains Pfam profiles
PF04563; RNA polymerase beta subunit, PF04560: RNA
polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2
domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF00562:
RNA polymerase Rpb2 domain 6
Length = 1114
Score = 181 bits (440), Expect = 5e-46
Identities = 101/254 (39%), Positives = 138/254 (54%), Gaps = 33/254 (12%)
Frame = +3
Query: 24 DMPFT-CEGITPDIIINPHAIPSRMTIGHLIECIQGKVSSNKGEIGDATPFNDAVN---- 188
DMPF G+ PD+IINPHA PSRMTI L+E I K S G+ DATPF DAV
Sbjct: 853 DMPFNGVTGMRPDLIINPHAFPSRMTIAMLLESIAAKGGSLHGKFVDATPFRDAVKKTNG 912
Query: 189 ---------VQKISSLLQDYGYHLRGNEVMYNGHTGRKINAQVFLGPTYYQRLKHMVDDK 341
V + S+L++ G++ G E +Y+G+ G ++ ++F+GP YYQRL+HMV DK
Sbjct: 913 EEESKSSLLVDDLGSMLKEKGFNHYGTETLYSGYLGVELKCEIFMGPVYYQRLRHMVSDK 972
Query: 342 IHSRARGPVQILVRQPMEGRARDGGLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHV 521
R+ G V L QP++GR R GG+RFGEMERD +AHGA+ L +RL SD + V
Sbjct: 973 FQVRSTGQVDQLTHQPIKGRKRGGGIRFGEMERDSLLAHGASYLLHDRLHTSSDHHIADV 1032
Query: 522 CNFCGLIAIANLRN-------------------NTFECKGCKNKTQISQIRLPYAAKLLF 644
C+ CG + +++ N C CK + + +PY + L
Sbjct: 1033 CSLCGSLLTSSVVNVQQKKLIQEIGKLPPGRTPKKVTCYSCKTSKGMETVAMPYVFRYLA 1092
Query: 645 QELMSMNIAPRLMV 686
EL SMNI L +
Sbjct: 1093 AELASMNIKMTLQL 1106
>At1g72040.1 68414.m08327 deoxynucleoside kinase family contains
Pfam profile: PF01712 deoxynucleoside kinase
Length = 580
Score = 31.5 bits (68), Expect = 0.61
Identities = 16/39 (41%), Positives = 21/39 (53%)
Frame = +1
Query: 346 ILELEVQYKF**DSLWKVGQEMVDYVLVKWNVIVRLHTE 462
+ EL+ YK D WK Q+MVDY+ +I R H E
Sbjct: 202 VAELKKLYK---DKFWKASQKMVDYLRSSVGIIHRNHAE 237
>At1g70020.1 68414.m08058 hypothetical protein
Length = 225
Score = 29.9 bits (64), Expect = 1.9
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +1
Query: 427 VKWNVIVRLHTELPNFYVNVYLKYQILTVYTCVISAA*SL 546
VKW++++RL ++LP +Y+ + Q +Y V A SL
Sbjct: 92 VKWDLVIRLPSDLPGYYMCLKGDLQTFILYKGVTIANSSL 131
>At4g13150.1 68417.m02047 expressed protein
Length = 119
Score = 28.7 bits (61), Expect = 4.3
Identities = 12/40 (30%), Positives = 22/40 (55%)
Frame = +3
Query: 144 KGEIGDATPFNDAVNVQKISSLLQDYGYHLRGNEVMYNGH 263
KG G +PF + ++ K+SS + + ++ GN M G+
Sbjct: 50 KGSGGGGSPFEEVKSLAKVSSSSEGFTFNAFGNRFMIPGN 89
>At3g46640.1 68416.m05063 myb family transcription factor contains
Pfam profile: PF00249 myb-like DNA-binding domain
Length = 323
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 3/38 (7%)
Frame = -3
Query: 513 YGKDLILQINV---HVKIGQLRVQSDNHVPFHQNVIHH 409
YG ++Q+ V H+ + Q+ NH P+HQN HH
Sbjct: 248 YGTQQMMQMPVYAHHMGMQGYHHQNHNHDPYHQNHRHH 285
>At1g02910.1 68414.m00258 tetratricopeptide repeat (TPR)-containing
protein contains Pfam profile PF00515: TPR Domain
Length = 453
Score = 28.7 bits (61), Expect = 4.3
Identities = 14/44 (31%), Positives = 21/44 (47%)
Frame = -2
Query: 499 DTSNKRSRKNWAAPCAI*QSRSISPKRNPPSLALPSIGCLTRIC 368
D + SR+NW + + S S SP +PPS P+ +C
Sbjct: 35 DATLFNSRRNWDSHLFVYASSSSSPSSSPPSPNSPTDDLTAELC 78
>At5g58350.1 68418.m07306 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 571
Score = 28.3 bits (60), Expect = 5.7
Identities = 21/73 (28%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Frame = +3
Query: 396 GRARDGGLRFGEMERDCQIAH---GAAQFLRERLFEVSDPYRIHVCNFCGLIAIANLRNN 566
G+ + G L M RDC AH G +F+ L+E + I V +F G+ + +
Sbjct: 160 GQVKIGDLGLARMLRDCHSAHSIIGTPEFMAPELYEENYNELIDVYSF-GM-CFLEMITS 217
Query: 567 TFECKGCKNKTQI 605
F C + QI
Sbjct: 218 EFPYSECNHPAQI 230
>At3g07150.1 68416.m00852 hypothetical protein
Length = 199
Score = 28.3 bits (60), Expect = 5.7
Identities = 17/50 (34%), Positives = 22/50 (44%)
Frame = +3
Query: 414 GLRFGEMERDCQIAHGAAQFLRERLFEVSDPYRIHVCNFCGLIAIANLRN 563
GL G+ E D + HG L + SDP CN GLI L++
Sbjct: 78 GLSTGKHEADALLVHGKLSKLGTKRAR-SDPLEDFACNDLGLIKTKKLKD 126
>At5g58880.1 68418.m07377 hypothetical protein
Length = 1088
Score = 27.9 bits (59), Expect = 7.6
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = -2
Query: 502 SDTSNKRSRKNWAAPCAI*QSRSISPK 422
+DT N + + W C I S+ ISP+
Sbjct: 955 ADTQNSQDSQTWTQQCGIDSSQGISPR 981
>At1g73875.1 68414.m08555 endonuclease/exonuclease/phosphatase
family protein contains Pfam profile PF03372:
Endonuclease/Exonuclease/phosphatase family
Length = 454
Score = 27.9 bits (59), Expect = 7.6
Identities = 15/42 (35%), Positives = 21/42 (50%)
Frame = +3
Query: 294 LGPTYYQRLKHMVDDKIHSRARGPVQILVRQPMEGRARDGGL 419
L TY+ R VD H++ PV++L P + R GGL
Sbjct: 390 LATTYHSRFLGTVDYIWHTKELVPVRVLETLPADVLRRTGGL 431
>At1g15660.1 68414.m01880 expressed protein similar to CENPCA
protein (GI:11863170) {Zea mays}
Length = 705
Score = 27.9 bits (59), Expect = 7.6
Identities = 27/118 (22%), Positives = 49/118 (41%)
Frame = +3
Query: 84 PSRMTIGHLIECIQGKVSSNKGEIGDATPFNDAVNVQKISSLLQDYGYHLRGNEVMYNGH 263
PS + + + + I +N G + A+PFND+V V++ D H ++ H
Sbjct: 350 PSEVNVQPIAKDIPNTSPTNVGTVDVASPFNDSV-VKRSGE--DDSHIH----SGIHRSH 402
Query: 264 TGRKINAQVFLGPTYYQRLKHMVDDKIHSRARGPVQILVRQPMEGRARDGGLRFGEME 437
R N + + + R M+ + R +G ++ V G R+ G R + E
Sbjct: 403 LSRDGNPDICVMDSISNRSSAMLQKNVDMRTKGK-EVDVPMSESGANRNTGDRENDAE 459
>At1g64810.1 68414.m07348 expressed protein contains Pfam PF05634:
Arabidopsis thaliana protein of unknown function
(DUF794)
Length = 436
Score = 27.5 bits (58), Expect = 10.0
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +3
Query: 513 IHVCNFCGLIAIANLRNNTFECKGCKN 593
+ C+ CG + +AN+ +N +C G N
Sbjct: 153 VFACSECGAVHVANVGHNIRDCNGPTN 179
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,317,742
Number of Sequences: 28952
Number of extensions: 349501
Number of successful extensions: 930
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 881
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 923
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1653386488
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -