Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0327
(682 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 451 e-129
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 440 e-126
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 333 1e-93
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 300 9e-84
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 38 1e-04
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic ac... 21 8.2
AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase pro... 21 8.2
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 451 bits (1112), Expect = e-129
Identities = 212/217 (97%), Positives = 213/217 (98%)
Frame = +2
Query: 26 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 205
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 206 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 385
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 386 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 565
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 566 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVE 676
IGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VE
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVE 217
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 440 bits (1084), Expect = e-126
Identities = 205/217 (94%), Positives = 210/217 (96%)
Frame = +2
Query: 26 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 205
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 206 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 385
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 386 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKK 565
GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMD T+PPYSE RFEEIKKEVSSYIKK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 566 IGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVE 676
IGYN A+VAFVPISGWHGDNMLEPS K PW+KGW+VE
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVE 217
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 333 bits (818), Expect = 1e-93
Identities = 156/160 (97%), Positives = 157/160 (98%)
Frame = +2
Query: 197 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 376
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 377 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSY 556
AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 557 IKKIGYNPAAVAFVPISGWHGDNMLEPSTKMPWFKGWQVE 676
IKKIGYNPAAVAFVPISGWHGDNMLE S+KMPWFKGW VE
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVE 160
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 300 bits (736), Expect = 9e-84
Identities = 140/144 (97%), Positives = 141/144 (97%)
Frame = +2
Query: 245 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 424
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 425 REHALLAFTLGVKQLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPI 604
REHALLAFTLGVKQLIVGVNKMDSTEPPYSE RFEEIKKEVSSYIKKIGYNPAAVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 605 SGWHGDNMLEPSTKMPWFKGWQVE 676
SGWHGDNMLE S+KMPWFKGW VE
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVE 144
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 37.5 bits (83), Expect = 1e-04
Identities = 36/117 (30%), Positives = 52/117 (44%)
Frame = +2
Query: 284 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVK 463
VT +D PGH FI G D VL+VAA G E QT + +A V
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV- 246
Query: 464 QLIVGVNKMDSTEPPYSEPRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLE 634
+IV +NK+D + ++E K + I+++G + V IS G N+ E
Sbjct: 247 PIIVAINKIDKPNIDIIKVQYELAKHGI--VIEELG---GEIQCVKISALKGINLRE 298
Score = 25.4 bits (53), Expect = 0.51
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 38 KTHINIVVIGHVDSGKST 91
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 47 INIVVIGHVDSGKST 91
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 269 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 370
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 269 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 370
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>AY569781-1|AAS75781.1| 461|Apis mellifera neuronal nicotinic
acetylcholine Apisa7-2 subunit protein.
Length = 461
Score = 21.4 bits (43), Expect = 8.2
Identities = 8/24 (33%), Positives = 13/24 (54%)
Frame = -1
Query: 646 FG*RLQHVVSVPSRNGHESDSSWV 575
FG L H++ V +N + + WV
Sbjct: 35 FGLSLHHIIDVDEKNQILTTNCWV 58
>AB253416-1|BAE86927.1| 580|Apis mellifera alpha-glucosidase
protein.
Length = 580
Score = 21.4 bits (43), Expect = 8.2
Identities = 7/20 (35%), Positives = 13/20 (65%)
Frame = +3
Query: 489 WIPLNHHTVSPDLRKSRRKY 548
W+P+N + S +L +R+Y
Sbjct: 443 WLPVNENYKSLNLAAQKREY 462
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 201,608
Number of Sequences: 438
Number of extensions: 4199
Number of successful extensions: 17
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 17
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20708550
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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