BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0322 (815 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz... 28 1.8 SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 27 4.2 SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p... 26 5.6 SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.4 >SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schizosaccharomyces pombe|chr 2|||Manual Length = 770 Score = 27.9 bits (59), Expect = 1.8 Identities = 11/23 (47%), Positives = 16/23 (69%) Frame = +3 Query: 75 RNADARNRLDSGKTSWTSRLLHY 143 RN +DSGKT++T R+L+Y Sbjct: 60 RNIGISAHIDSGKTTFTERVLYY 82 >SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizosaccharomyces pombe|chr 1|||Manual Length = 1290 Score = 26.6 bits (56), Expect = 4.2 Identities = 17/37 (45%), Positives = 19/37 (51%) Frame = +2 Query: 245 EELAPKNEAAN**KRTDTNKASLRTLKTLMLTKGIKR 355 E P +E AN KR D SLR K L KG+KR Sbjct: 159 ESFWPLSEPANLKKRFDEIFESLRYAKALDQIKGLKR 195 >SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces pombe|chr 2|||Manual Length = 1040 Score = 26.2 bits (55), Expect = 5.6 Identities = 25/78 (32%), Positives = 35/78 (44%) Frame = +2 Query: 176 GTKTVKSFQLRMELLELPRAVRGEELAPKNEAAN**KRTDTNKASLRTLKTLMLTKGIKR 355 GT T L + LL L +A L EAA D+ +A R + + + + R Sbjct: 680 GTITTHYGSLLVLLLRLRQACCHPWLIVAREAAV--DDNDSFQAKNRAIYNQIYPEAVNR 737 Query: 356 LPKKCVPTLTCSICPMVV 409 L K + TL CS+C VV Sbjct: 738 L--KLIETLQCSLCMDVV 753 >SPBC713.09 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 395 Score = 25.8 bits (54), Expect = 7.4 Identities = 24/96 (25%), Positives = 42/96 (43%) Frame = +1 Query: 130 GSYTIEKSDGSGFTERYQNSEVVPVENGVIRTAESGERGGTCSEERSSELIKADGYEQSV 309 G+ + + G+ T+ +N+E + N V +AE E + +S E ++V Sbjct: 43 GNNQVNEPTGNDNTQVVENTEDISASNVV--SAEGAEASTGDASTQSPET------SENV 94 Query: 310 VKNVKNANAHERNQKATEEVRTDTDVQHLPNGGIAK 417 VKN + + E+ +K V D P+G I K Sbjct: 95 VKNSVDESVAEKPEKEDLAVIESEDKAAKPDGEIKK 130 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,901,187 Number of Sequences: 5004 Number of extensions: 54417 Number of successful extensions: 158 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 151 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 158 length of database: 2,362,478 effective HSP length: 72 effective length of database: 2,002,190 effective search space used: 398435810 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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