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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0322
         (815 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g49250.1 68414.m05522 ATP dependent DNA ligase family protein...    31   0.69 
At3g26990.1 68416.m03377 expressed protein contains Pfam domain,...    31   0.91 
At5g64813.1 68418.m08152 GTP-binding protein-related contains we...    30   2.1  
At3g13460.2 68416.m01694 expressed protein contains Pfam profile...    28   6.4  
At3g13460.1 68416.m01693 expressed protein contains Pfam profile...    28   6.4  
At5g10660.1 68418.m01234 calmodulin-binding protein-related cont...    28   8.5  

>At1g49250.1 68414.m05522 ATP dependent DNA ligase family protein
           contains Pfam profile: PF01068 ATP dependent DNA ligase
           domain
          Length = 657

 Score = 31.5 bits (68), Expect = 0.69
 Identities = 14/43 (32%), Positives = 24/43 (55%)
 Frame = +2

Query: 332 MLTKGIKRLPKKCVPTLTCSICPMVVLPKLRLRLLFEEWEQPI 460
           +LT G+  LPK C  TL   + PM+     R+ L+ E+++  +
Sbjct: 265 LLTSGVWNLPKTCNLTLGVPVRPMLAKATTRVDLILEKFKDTV 307


>At3g26990.1 68416.m03377 expressed protein contains Pfam domain,
           PF04818: Protein of unknown function, DUF618
          Length = 513

 Score = 31.1 bits (67), Expect = 0.91
 Identities = 27/108 (25%), Positives = 45/108 (41%), Gaps = 1/108 (0%)
 Frame = +1

Query: 97  VWIPEKPVGRRGSYTIEKSDGSGFTERYQNSEVVPVENGVIRTAESGERGGTCSEERSS- 273
           +W   K  G RG    E+  G       +N  +VP++  V    +    G T  +  S+ 
Sbjct: 111 IWEERKVFGSRGQILKEELLGRQPENGTRNGNLVPLKLSV---PQRQVNGSTLEKVVSAV 167

Query: 274 ELIKADGYEQSVVKNVKNANAHERNQKATEEVRTDTDVQHLPNGGIAK 417
           E++     ++  +   K+ NA    +KAT+EV  D    H P   + K
Sbjct: 168 EVLHGVQIDEDALVG-KSTNAAGYLEKATQEVERDLSSGHAPGPAVVK 214


>At5g64813.1 68418.m08152 GTP-binding protein-related contains weak
           similarity to Ras-related protein Rab-27A (Rab-27)
           (GTP-binding protein Ram) (Swiss-Prot:P51159) [Homo
           sapiens]
          Length = 342

 Score = 29.9 bits (64), Expect = 2.1
 Identities = 15/47 (31%), Positives = 21/47 (44%)
 Frame = +1

Query: 46  STTVTPEPVQETLTQEIVWIPEKPVGRRGSYTIEKSDGSGFTERYQN 186
           + T+ P P Q  LT      P++PV    +YTI +   S   E   N
Sbjct: 284 NNTIPPLPAQRNLTPPPTLYPQQPVSTPDNYTIPRYSLSSVQETTNN 330


>At3g13460.2 68416.m01694 expressed protein contains Pfam profile
           PF04146: YT521-B-like family
          Length = 664

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 15/59 (25%), Positives = 31/59 (52%)
 Frame = +1

Query: 241 RGGTCSEERSSELIKADGYEQSVVKNVKNANAHERNQKATEEVRTDTDVQHLPNGGIAK 417
           R  T  E+++ +  K    E+   +  ++A A   ++++   V+T +DV+   NG +AK
Sbjct: 595 RQKTILEKKAKQTQKQVSEEKVTDEKKESATAESASKESPAAVQTSSDVKVAENGSVAK 653


>At3g13460.1 68416.m01693 expressed protein contains Pfam profile
           PF04146: YT521-B-like family
          Length = 667

 Score = 28.3 bits (60), Expect = 6.4
 Identities = 15/59 (25%), Positives = 31/59 (52%)
 Frame = +1

Query: 241 RGGTCSEERSSELIKADGYEQSVVKNVKNANAHERNQKATEEVRTDTDVQHLPNGGIAK 417
           R  T  E+++ +  K    E+   +  ++A A   ++++   V+T +DV+   NG +AK
Sbjct: 598 RQKTILEKKAKQTQKQVSEEKVTDEKKESATAESASKESPAAVQTSSDVKVAENGSVAK 656


>At5g10660.1 68418.m01234 calmodulin-binding protein-related
           contains weak similarity to calmodulin-binding proteins
          Length = 407

 Score = 27.9 bits (59), Expect = 8.5
 Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 5/57 (8%)
 Frame = +1

Query: 229 ESGERGGTC-----SEERSSELIKADGYEQSVVKNVKNANAHERNQKATEEVRTDTD 384
           ESGE   T      +EE+  ELI  D   + + +  +  N  E N +  EEV+  +D
Sbjct: 238 ESGEEKETSPVAASTEEQKGELIDEDKSTEQIEEPKEPENIEENNSEEEEEVKKKSD 294


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,766,270
Number of Sequences: 28952
Number of extensions: 276898
Number of successful extensions: 826
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 802
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 826
length of database: 12,070,560
effective HSP length: 80
effective length of database: 9,754,400
effective search space used: 1863090400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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