Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0318
(735 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g08100.2 68418.m00944 L-asparaginase / L-asparagine amidohydr... 96 2e-20
At5g08100.1 68418.m00945 L-asparaginase / L-asparagine amidohydr... 96 2e-20
At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine... 88 7e-18
At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine... 88 7e-18
At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine... 87 1e-17
At4g00590.1 68417.m00083 asparaginase 2 family protein very low ... 43 2e-04
At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi do... 37 0.016
At4g27850.1 68417.m03999 proline-rich family protein contains pr... 31 0.79
At4g38050.1 68417.m05374 xanthine/uracil permease family protein... 30 1.8
At4g29020.1 68417.m04149 glycine-rich protein supporting cDNA gi... 30 1.8
At4g22070.1 68417.m03192 WRKY family transcription factor identi... 29 3.2
At1g04660.1 68414.m00463 glycine-rich protein 29 3.2
At5g02510.1 68418.m00185 hypothetical protein 29 4.2
At1g62300.1 68414.m07028 WRKY family transcription factor simila... 29 4.2
At3g63300.2 68416.m07118 expressed protein 28 7.4
At3g63300.1 68416.m07117 expressed protein 28 7.4
At5g47800.1 68418.m05904 phototropic-responsive NPH3 family prot... 27 9.8
At5g06805.1 68418.m00769 reverse transcriptase-related low simil... 27 9.8
At4g04450.1 68417.m00647 WRKY family transcription factor simila... 27 9.8
At4g01985.1 68417.m00265 expressed protein 27 9.8
At3g46380.1 68416.m05024 hypothetical protein 27 9.8
>At5g08100.2 68418.m00944 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 235
Score = 95.9 bits (228), Expect = 2e-20
Identities = 56/127 (44%), Positives = 76/127 (59%), Gaps = 1/127 (0%)
Frame = -1
Query: 624 VGTVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGESI 445
+GTVG VA+D+ G++A ATSTGG KMVGRIGDTP+IG GTYA +++ IS TG GE I
Sbjct: 101 IGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYA-NHLCAISATGKGEDI 159
Query: 444 LKYCLAHTIIKLME-GGLDADTATRQAVNGMTARLNNTAGAITLSKNGDVGIHFSFKRMA 268
++ +A + LME GL A V+ R + G + +S NG+V + F+ M
Sbjct: 160 IRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPR--GSCGLVAVSANGEVTMPFNTTGMF 217
Query: 267 WAYVKND 247
A D
Sbjct: 218 RACASED 224
>At5g08100.1 68418.m00945 L-asparaginase / L-asparagine
amidohydrolase identical to Swiss-Prot:P50287
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Arabidopsis thaliana]
Length = 315
Score = 95.9 bits (228), Expect = 2e-20
Identities = 56/127 (44%), Positives = 76/127 (59%), Gaps = 1/127 (0%)
Frame = -1
Query: 624 VGTVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGESI 445
+GTVG VA+D+ G++A ATSTGG KMVGRIGDTP+IG GTYA +++ IS TG GE I
Sbjct: 181 IGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYA-NHLCAISATGKGEDI 239
Query: 444 LKYCLAHTIIKLME-GGLDADTATRQAVNGMTARLNNTAGAITLSKNGDVGIHFSFKRMA 268
++ +A + LME GL A V+ R + G + +S NG+V + F+ M
Sbjct: 240 IRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPR--GSCGLVAVSANGEVTMPFNTTGMF 297
Query: 267 WAYVKND 247
A D
Sbjct: 298 RACASED 304
>At5g61540.2 68418.m07723 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 257
Score = 87.8 bits (208), Expect = 7e-18
Identities = 38/106 (35%), Positives = 63/106 (59%), Gaps = 1/106 (0%)
Frame = -1
Query: 618 TVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGESILK 439
T+ ID GHIAV TST G K+ GR+GD P++G YADD VGG TG G+++++
Sbjct: 122 TISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMR 181
Query: 438 YCLAHTIIKLMEGGLDADTATRQAVNGMTARLNNTAGA-ITLSKNG 304
+ + +++ M G+ + A + A++ + + + GA + + KNG
Sbjct: 182 FLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNG 227
>At5g61540.1 68418.m07722 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:O02467
N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase)
(Aspartylglucosaminidase)(N4-(N-acetyl-beta-
glucosaminyl)-L-asparagine amidase) (AGA)[Spodoptera
frugiperda]
Length = 359
Score = 87.8 bits (208), Expect = 7e-18
Identities = 38/106 (35%), Positives = 63/106 (59%), Gaps = 1/106 (0%)
Frame = -1
Query: 618 TVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGESILK 439
T+ ID GHIAV TST G K+ GR+GD P++G YADD VGG TG G+++++
Sbjct: 224 TISMAVIDRMGHIAVGTSTNGATYKIPGRVGDGPIVGSSAYADDEVGGCGATGDGDTMMR 283
Query: 438 YCLAHTIIKLMEGGLDADTATRQAVNGMTARLNNTAGA-ITLSKNG 304
+ + +++ M G+ + A + A++ + + + GA + + KNG
Sbjct: 284 FLPCYQVVESMRQGMKPEEAAKDAISRIARKFPDFVGAVVAVDKNG 329
>At3g16150.1 68416.m02039 L-asparaginase, putative / L-asparagine
amidohydrolase, putative similar to Swiss-Prot:P30364
L-asparaginase (EC 3.5.1.1) (L-asparagine
amidohydrolase) [Lupinus angustifolius]
Length = 325
Score = 87.0 bits (206), Expect = 1e-17
Identities = 52/118 (44%), Positives = 69/118 (58%), Gaps = 2/118 (1%)
Frame = -1
Query: 618 TVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGESILK 439
TVG V +D GH A TSTGG+ KM+GRIGD+PLIG GTYA + G+S TG GE+I++
Sbjct: 195 TVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASE-FCGVSCTGEGEAIIR 253
Query: 438 YCLAHTIIKLME-GGLDADTATRQAVNGMTARLN-NTAGAITLSKNGDVGIHFSFKRM 271
LA + +ME GL+ A + RL+ AG I +S G+V F+ M
Sbjct: 254 ATLARDVSAVMEYKGLNLQEAVDYVIK---HRLDEGFAGLIAVSNKGEVVCGFNSNGM 308
>At4g00590.1 68417.m00083 asparaginase 2 family protein very low
similarity to glycosylasparaginase [SP|P20933] from Homo
sapiens; contains Pfam profile PF01112: Asparaginase 2
Length = 408
Score = 43.2 bits (97), Expect = 2e-04
Identities = 19/42 (45%), Positives = 27/42 (64%)
Frame = -1
Query: 618 TVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYA 493
TVG + +D +GHIA +S+GGI K+ GR+G G G +A
Sbjct: 222 TVGVICVDNEGHIACGSSSGGIAMKISGRVGLAATYGSGCWA 263
>At1g31290.1 68414.m03829 PAZ domain-containing protein / piwi
domain-containing protein contains Pfam profiles
PF02170: PAZ domain, PF02171: Piwi domain
Length = 1194
Score = 36.7 bits (81), Expect = 0.016
Identities = 23/74 (31%), Positives = 30/74 (40%), Gaps = 1/74 (1%)
Frame = -1
Query: 669 GDDQRTEIGRKDEGGVGTVGPVAIDADGHIAVA-TSTGGINGKMVGRIGDTPLIGGGTYA 493
GD R +G GVG VG + G + V GG+ G +GD G G
Sbjct: 165 GDVGRDGVGDVGRDGVGDVGQGGVGDVGQVGVGDVGQGGVGDVGQGGVGDVGRDGVGDVG 224
Query: 492 DDNVGGISTTGHGE 451
D VG + G G+
Sbjct: 225 RDGVGDVGRGGVGD 238
Score = 35.5 bits (78), Expect = 0.037
Identities = 25/96 (26%), Positives = 39/96 (40%), Gaps = 1/96 (1%)
Frame = -1
Query: 735 QVPPESLISESAKEALNDFLTKGDDQRTEIGRKDEGGVGTVGPVAIDADGHIAVA-TSTG 559
Q PP+ + S++ ++ + GD + +G GVG VG + G V
Sbjct: 135 QPPPQEVPPSSSQAQVSQGVAPGDVGQGGVGDVGRDGVGDVGRDGVGDVGQGGVGDVGQV 194
Query: 558 GINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGE 451
G+ G +GD G G D VG + G G+
Sbjct: 195 GVGDVGQGGVGDVGQGGVGDVGRDGVGDVGRDGVGD 230
>At4g27850.1 68417.m03999 proline-rich family protein contains
proline-rich extensin domains, INTERPRO:IPR002965
Length = 577
Score = 31.1 bits (67), Expect = 0.79
Identities = 16/41 (39%), Positives = 22/41 (53%)
Frame = +2
Query: 245 LSFFT*AQAILLKLKWIPTSPFFDNVIAPAVLFKRAVIPFT 367
LS F+ +L L W SP F+N+IA + + VIP T
Sbjct: 306 LSVFSLLFLAILTLPWASISPIFNNLIAAIKTWSKRVIPQT 346
>At4g38050.1 68417.m05374 xanthine/uracil permease family protein
contains Pfam profile: PF00860 permease family
Length = 703
Score = 29.9 bits (64), Expect = 1.8
Identities = 12/36 (33%), Positives = 23/36 (63%)
Frame = +1
Query: 160 KDTFRFLVTAVILKGFFKCLIVFYSVVDLIVLHISP 267
+DT R L A+I+ F+C++ F ++ L++ I+P
Sbjct: 278 RDTMRELQGAIIVGSLFQCILGFSGLMSLLLRFINP 313
>At4g29020.1 68417.m04149 glycine-rich protein supporting cDNA
gi|20465684|gb|AY096677.1|
Length = 158
Score = 29.9 bits (64), Expect = 1.8
Identities = 24/76 (31%), Positives = 33/76 (43%), Gaps = 5/76 (6%)
Frame = -1
Query: 666 DDQRTEIGRKDEGGVGTVGPVAIDADGHIAVATSTGGINGKM-VGRI-GDTPLIGGGTYA 493
DDQ+ + GG +G G VA GG+ G + VG + G +GGG
Sbjct: 35 DDQKNFVAFAGVGGAAGIGGAGGVGAGLGGVAGGVGGVAGVLPVGGVGGGIGGLGGGVGG 94
Query: 492 DDNVGGI---STTGHG 454
+GG+ S GHG
Sbjct: 95 LGGLGGLGGGSGLGHG 110
>At4g22070.1 68417.m03192 WRKY family transcription factor identical
to WRKY transcription factor 31 (WRKY31) GI:15990589
from [Arabidopsis thaliana]
Length = 538
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/35 (37%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = +2
Query: 314 DNVIAPAVLFKRAVIPFTACLVAVSASRP-PSISL 415
D ++ P L RA++P ++ + +SAS P P+I+L
Sbjct: 383 DGLMNPTNLLARAILPCSSSMATISASAPFPTITL 417
>At1g04660.1 68414.m00463 glycine-rich protein
Length = 212
Score = 29.1 bits (62), Expect = 3.2
Identities = 21/62 (33%), Positives = 27/62 (43%), Gaps = 5/62 (8%)
Frame = -1
Query: 630 GGVGTVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPL-----IGGGTYADDNVGGIST 466
GGVG +G V G V GG+ G VG +G L +GGG +GG+
Sbjct: 114 GGVGGLGGVGGGVGGLGGVGGGVGGLGG--VGGLGGAGLGGVGGVGGGIGKAGGIGGLGG 171
Query: 465 TG 460
G
Sbjct: 172 LG 173
Score = 27.9 bits (59), Expect = 7.4
Identities = 19/60 (31%), Positives = 26/60 (43%), Gaps = 1/60 (1%)
Frame = -1
Query: 630 GGVGTVGPVAIDAD-GHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHG 454
GG+G G + A G GG+ G + G +G +GGG VGG+ G G
Sbjct: 93 GGIGKYGGIGGAAGIGGFHSIGGVGGLGG-VGGGVGGLGGVGGGVGGLGGVGGLGGAGLG 151
>At5g02510.1 68418.m00185 hypothetical protein
Length = 179
Score = 28.7 bits (61), Expect = 4.2
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -1
Query: 723 ESLISESAKEALNDFLTKGDDQRTEIG 643
++ + E AKE+L+ L GD RTE G
Sbjct: 4 KNYVFEEAKESLSSILFNGDSDRTEAG 30
>At1g62300.1 68414.m07028 WRKY family transcription factor similar
to putative DNA-binding protein GI:7268215 from
[Arabidopsis thaliana]
Length = 553
Score = 28.7 bits (61), Expect = 4.2
Identities = 13/35 (37%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +2
Query: 314 DNVIAPAVLFKRAVIPFTACLVAVSASRP-PSISL 415
D ++ P L RAV+P + + +SAS P P+++L
Sbjct: 398 DGMMNPTNLLARAVLPCSTSMATISASAPFPTVTL 432
>At3g63300.2 68416.m07118 expressed protein
Length = 382
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/48 (27%), Positives = 22/48 (45%)
Frame = -1
Query: 519 PLIGGGTYADDNVGGISTTGHGESILKYCLAHTIIKLMEGGLDADTAT 376
PL GGG++ + + IS + + ++KY H I + G T
Sbjct: 18 PLNGGGSFTETDSPPISPSDEFDDVIKYFRTHNTIHPLFSGTGGSRGT 65
>At3g63300.1 68416.m07117 expressed protein
Length = 498
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/48 (27%), Positives = 22/48 (45%)
Frame = -1
Query: 519 PLIGGGTYADDNVGGISTTGHGESILKYCLAHTIIKLMEGGLDADTAT 376
PL GGG++ + + IS + + ++KY H I + G T
Sbjct: 134 PLNGGGSFTETDSPPISPSDEFDDVIKYFRTHNTIHPLFSGTGGSRGT 181
>At5g47800.1 68418.m05904 phototropic-responsive NPH3 family protein
contains NPH3 family domain, Pfam:PF03000
Length = 559
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +3
Query: 459 DLLLKSHQHCHQHKYRHQLVA 521
DLLL SH H H+Y LVA
Sbjct: 324 DLLLPSHSSSHLHRYDTDLVA 344
>At5g06805.1 68418.m00769 reverse transcriptase-related low
similarity to reverse transcriptase [Arabidopsis
thaliana] GI:976278
Length = 594
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/29 (41%), Positives = 15/29 (51%), Gaps = 1/29 (3%)
Frame = -3
Query: 613 RSGSYRR*WPYSCCYKHWW-YKWKNGRTY 530
RS S RR WP WW +KW+ G +
Sbjct: 424 RSDSERRSWPTLFALTVWWGWKWRCGYVF 452
>At4g04450.1 68417.m00647 WRKY family transcription factor similar
to A. fatua wild oat ABF2 DNA-binding protein, GenBank
accession number S61414
Length = 528
Score = 27.5 bits (58), Expect = 9.8
Identities = 12/35 (34%), Positives = 22/35 (62%), Gaps = 1/35 (2%)
Frame = +2
Query: 314 DNVIAPAVLFKRAVIPFTACLVAVSASRP-PSISL 415
D ++ P L R ++P ++ + +SAS P P+I+L
Sbjct: 379 DGLMNPTNLLARTILPCSSSMATISASAPFPTITL 413
>At4g01985.1 68417.m00265 expressed protein
Length = 579
Score = 27.5 bits (58), Expect = 9.8
Identities = 19/62 (30%), Positives = 23/62 (37%)
Frame = -1
Query: 630 GGVGTVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGISTTGHGE 451
GG G G A G + GG G VG G GGGT G +G G
Sbjct: 159 GGKGRGGKSGGGAGGGVGGGVGAGGGAGGSVGAGGGIGSGGGGTVGAGGRGSGGASGGGG 218
Query: 450 SI 445
++
Sbjct: 219 TV 220
>At3g46380.1 68416.m05024 hypothetical protein
Length = 117
Score = 27.5 bits (58), Expect = 9.8
Identities = 21/68 (30%), Positives = 27/68 (39%)
Frame = -1
Query: 645 GRKDEGGVGTVGPVAIDADGHIAVATSTGGINGKMVGRIGDTPLIGGGTYADDNVGGIST 466
G KD G G +D DG + +S N V R GD G DD GG T
Sbjct: 21 GNKDRTDGGVEG--GVDDDGAMVERSSLRFNND--VNRSGDVDSSNSGGNDDDGSGGCGT 76
Query: 465 TGHGESIL 442
G + ++
Sbjct: 77 NGDVDVVI 84
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,293,816
Number of Sequences: 28952
Number of extensions: 273974
Number of successful extensions: 877
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 824
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 866
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1614253080
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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