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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0309
         (848 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC21D10.08c |||sequence orphan|Schizosaccharomyces pombe|chr 2...    33   0.039
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce...    30   0.48 
SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomy...    29   0.63 
SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase |Schizosa...    29   1.1  
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k...    28   1.5  
SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces p...    27   3.4  
SPAC1002.10c |sgt1||SGT1 family transcriptional regulator Sgt1|S...    27   4.4  
SPAC13F5.06c |sec10||exocyst complex subunit Sec10|Schizosacchar...    26   5.9  
SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyce...    26   7.8  

>SPBC21D10.08c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 277

 Score = 33.5 bits (73), Expect = 0.039
 Identities = 42/178 (23%), Positives = 80/178 (44%), Gaps = 7/178 (3%)
 Frame = +2

Query: 305 NDNWNVVTYLNTNNIQSHLDKVDQLF---EKVNSFCKDFESSKIQFD-CMNA---ISSLQ 463
           +D WN+  YLN  +    LDK+  ++   +  N   K+ ++ KI+ + C +    +SS+ 
Sbjct: 13  DDGWNLKNYLNIQSTSLCLDKLCNIYTQGQDENEDAKELQAIKIKIEWCKDTSVLLSSIC 72

Query: 464 SQHDNNIKKFASVSYLTSSRQSRSKRGLLDFGGSVLKTFFGTLDSNDGVKFSDAIDQVQS 643
               ++I  F  V     +++S SKR   D+   +  T F    +   ++        + 
Sbjct: 73  VMLLDSIHDFL-VQQGKITKESISKR---DYSHEI--TIFNFKYAQQEMQNEKLYHFAKL 126

Query: 644 DEKALAHLMRDNIHVIKSTISTFNNSMLKFSENEKRLNKNLEIINSAFEYIINSTDKF 817
            E AL  L   N H+  +TI      + + S +E  L+  +E ++    Y  +S++KF
Sbjct: 127 LEPALESLKVTNNHITHATI------VGQVSGSEHNLSTAIEQVDVIVNYFYDSSEKF 178


>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1957

 Score = 29.9 bits (64), Expect = 0.48
 Identities = 32/147 (21%), Positives = 62/147 (42%), Gaps = 1/147 (0%)
 Frame = +2

Query: 338  TNNIQSHLDKVDQL-FEKVNSFCKDFESSKIQFDCMNAISSLQSQHDNNIKKFASVSYLT 514
            T  ++    K+ QL  E+ N   K+   S    D    +  L+  + + IKK   V  L 
Sbjct: 629  TKQLKDTSSKLQQLQLERANFEQKESTLSDENNDLRTKLLKLEESNKSLIKKQEDVDSLE 688

Query: 515  SSRQSRSKRGLLDFGGSVLKTFFGTLDSNDGVKFSDAIDQVQSDEKALAHLMRDNIHVIK 694
             + Q+  +    D   S     F  L++ +  +  D +       +A  + +  ++   K
Sbjct: 689  KNIQTLKE----DLRKSEEALRFSKLEAKNLREVIDNLKGKHETLEAQRNDLHSSLSDAK 744

Query: 695  STISTFNNSMLKFSENEKRLNKNLEII 775
            +T +  ++ + K SE+ KRL  N+E +
Sbjct: 745  NTNAILSSELTKSSEDVKRLTANVETL 771


>SPAC23G3.02c |sib1||ferrichrome synthetase Sib1|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 4924

 Score = 29.5 bits (63), Expect = 0.63
 Identities = 24/85 (28%), Positives = 41/85 (48%)
 Frame = +2

Query: 518  SRQSRSKRGLLDFGGSVLKTFFGTLDSNDGVKFSDAIDQVQSDEKALAHLMRDNIHVIKS 697
            S+ + ++  LLD     ++ FF   ++ + V F   I  V  D  A   +M D  H+++ 
Sbjct: 1414 SKYNLAEIPLLDSKKVPIQIFFLQGENKNYVLF--CIHHVLYDAWAFQLIMDDINHLLRK 1471

Query: 698  TISTFNNSMLKFSENEKRLNKNLEI 772
                 + SMLKF     R NKN+++
Sbjct: 1472 ENPKGSQSMLKFISYLHRYNKNVDL 1496


>SPAC20H4.04 |mfh2||ATP-dependent 3' to 5' DNA helicase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 783

 Score = 28.7 bits (61), Expect = 1.1
 Identities = 22/74 (29%), Positives = 38/74 (51%), Gaps = 1/74 (1%)
 Frame = +2

Query: 464 SQHDNNIKKFASVSYLTSSRQSRSKRGLLDFGGSVLKTFFGTLDSNDGVKFSDA-IDQVQ 640
           + ++NN    + V+ L +   SRSKR  L F   ++   F  ++SN  V   DA I + +
Sbjct: 647 NSYNNNSSSLSPVNTLANQAHSRSKR-YLPF---IVDDVFEDMESNLRVPTEDAKIKRFK 702

Query: 641 SDEKALAHLMRDNI 682
           SD ++  +  R N+
Sbjct: 703 SDYRSCIYNARRNV 716


>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
           kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 836

 Score = 28.3 bits (60), Expect = 1.5
 Identities = 32/139 (23%), Positives = 55/139 (39%), Gaps = 6/139 (4%)
 Frame = +2

Query: 416 SSKIQFDCMNAISSLQSQHDNNIKKFASVSYLT-SSRQSRSKRGLLDFGGSVLKTFF--G 586
           +SKI  + +N++        N  K    +   +        K+GL  F    LK  F   
Sbjct: 61  NSKILTELINSVKRPNKPDQNEEKSAVGIEEKSFKDEHLAQKKGLHHFAD--LKEIFLNK 118

Query: 587 TLDSNDGVKFSDAIDQVQS---DEKALAHLMRDNIHVIKSTISTFNNSMLKFSENEKRLN 757
            L + DG++ +   D +QS   D   ++   R  +  + +  S+     +   EN   LN
Sbjct: 119 NLSTLDGLQDASLHDNIQSGKLDNPVISQNRRIVLEKLANPNSSKEYETIPTVENRHPLN 178

Query: 758 KNLEIINSAFEYIINSTDK 814
           K  +    A+E  IN  D+
Sbjct: 179 KLSKTKTLAYEETINQLDE 197


>SPBC23E6.02 |||ATP-dependent DNA helicase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1040

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 13/50 (26%), Positives = 24/50 (48%)
 Frame = +2

Query: 644 DEKALAHLMRDNIHVIKSTISTFNNSMLKFSENEKRLNKNLEIINSAFEY 793
           D  +  + M  NI  +       +NS++    NE++LN +L + N+   Y
Sbjct: 75  DSSSSLNTMESNIEKVNGLFPN-DNSVIALKPNEEKLNSSLSVENNDSTY 123


>SPAC1002.10c |sgt1||SGT1 family transcriptional regulator
           Sgt1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 590

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 34/154 (22%), Positives = 72/154 (46%), Gaps = 2/154 (1%)
 Frame = +2

Query: 335 NTNNIQSHLDKVDQLFEK-VNSFCKDF-ESSKIQFDCMNAISSLQSQHDNNIKKFASVSY 508
           +T+ +  + D++++L EK +NSFC DF +  +  FD  +  ++L  + +         S 
Sbjct: 347 DTSFMNVNPDELEELLEKKLNSFCDDFGDDERSGFDNTDHDNTLVGEEEMVPGNHGGKSI 406

Query: 509 LTSSRQSRSKRGLLDFGGSVLKTFFGTLDSNDGVKFSDAIDQVQSDEKALAHLMRDNIHV 688
                +++ K+   +F  S LK              +  I+   +DE +  H  R++ + 
Sbjct: 407 NEEITKNKQKQ---NFNESDLK------------NMASRIETFINDEASNNH--REDFYG 449

Query: 689 IKSTISTFNNSMLKFSENEKRLNKNLEIINSAFE 790
           +K++ +  ++  L  S++E  LN+N  I    F+
Sbjct: 450 VKNSDTDTDSDSLADSDDEIFLNRNQGIDEVEFD 483


>SPAC13F5.06c |sec10||exocyst complex subunit
           Sec10|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 811

 Score = 26.2 bits (55), Expect = 5.9
 Identities = 22/121 (18%), Positives = 53/121 (43%)
 Frame = +2

Query: 431 FDCMNAISSLQSQHDNNIKKFASVSYLTSSRQSRSKRGLLDFGGSVLKTFFGTLDSNDGV 610
           ++  N  S   S  D +IK FA+  +  +  +SR    + D+  +  +        N+  
Sbjct: 38  YETQNDGSKKLSSIDGSIKSFAACLHELNRLKSRVGDRIRDYASASKQV------QNEYH 91

Query: 611 KFSDAIDQVQSDEKALAHLMRDNIHVIKSTISTFNNSMLKFSENEKRLNKNLEIINSAFE 790
           + S+ + +  +    L+  + DN++ ++S +      + +   N KR+  + E++    E
Sbjct: 92  QKSNHLREKFAQVLELSRHLEDNVNDMRSGLVDAGQELERAENNRKRILSSAELLRYYLE 151

Query: 791 Y 793
           +
Sbjct: 152 F 152


>SPAC15A10.04c |zpr1||zinc finger protein Zpr1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 459

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 17/64 (26%), Positives = 32/64 (50%)
 Frame = +2

Query: 362 DKVDQLFEKVNSFCKDFESSKIQFDCMNAISSLQSQHDNNIKKFASVSYLTSSRQSRSKR 541
           D+++   EKVNS         I  D +   S ++ +   +  +++ VSY  +  Q+ +K 
Sbjct: 158 DQINAFIEKVNSLRSGSVPFTITVDDITGNSWIEMKPGRDGDRWSQVSYKRTLEQN-TKL 216

Query: 542 GLLD 553
           GL+D
Sbjct: 217 GLVD 220


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,042,450
Number of Sequences: 5004
Number of extensions: 59080
Number of successful extensions: 187
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 179
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 187
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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