Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0303
(727 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 69 9e-13
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 67 3e-12
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 60 2e-10
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 60 4e-10
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 48 1e-06
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 42 9e-05
SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 41 2e-04
SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit |... 31 0.17
SPAC25H1.03 |mug66|mug66|meiotically upregulated gene Mug66|Schi... 26 4.8
SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 26 6.3
SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|c... 25 8.3
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 68.5 bits (160), Expect = 9e-13
Identities = 44/110 (40%), Positives = 66/110 (60%), Gaps = 3/110 (2%)
Frame = +2
Query: 2 PLALSLLPMAGAIAAGNTVIVKPSEISPASA-RFIVEYIPKYLDNDAVIIIEGGPQETTK 178
PL ++L + AIA+GN V++KPSE++P S F + L + + +I G +E+
Sbjct: 175 PLKMALWKLVPAIASGNCVVLKPSELAPWSCLEFALICKEAGLPDGVLNVIIGSGKESGA 234
Query: 179 LLS-H-RFDYIFYTGSTNIGKIIYASAAKHLTPVTLELGGKSRLQAVLDA 322
LS H + Y+ +TGS GK I +AA+++ P+TLELGGKS L DA
Sbjct: 235 ALSCHPKIAYLAFTGSLATGKKIMHAAAENIVPLTLELGGKSPLIICEDA 284
Score = 57.2 bits (132), Expect = 2e-09
Identities = 36/105 (34%), Positives = 56/105 (53%), Gaps = 2/105 (1%)
Frame = +2
Query: 332 KIAIGG--KYDPGDKFIDLTILTNVDVNDKIMEDEIFGPLLAICVVKDINEALKFINERG 505
K +GG + + FI T+ TNV ++KI +EIFGP+LA+ EAL+ N+
Sbjct: 369 KCVVGGLPRSEQKGYFIPPTVFTNVQTHNKIWREEIFGPVLAVKTFHTNEEALELANDSE 428
Query: 506 KPLVLYVFSSSEKVHKIFASHTSSGSICFNDTIVFTGVHTLPFGG 640
L VFS++ K + F+++ +G N+ V T H LP+ G
Sbjct: 429 YGLGSGVFSTNPKTLEFFSNNIEAGMCSLNNYHVVT--HELPWIG 471
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 66.9 bits (156), Expect = 3e-12
Identities = 42/122 (34%), Positives = 69/122 (56%), Gaps = 4/122 (3%)
Frame = +2
Query: 2 PLALSLLPMAGAIAAGNTVIVKPSEISPASARFIVEYIPKYLDNDAVI-IIEG-GPQETT 175
P + +A A+A GNT+I+K +E++P SA + +++P+ VI ++ G G +
Sbjct: 173 PFLMCAWKIAPAVACGNTIILKTAELTPLSALCLTKFVPECGFPPGVINVLSGDGRRCGN 232
Query: 176 KLLSHR-FDYIFYTGSTNIGKIIY-ASAAKHLTPVTLELGGKSRLQAVLDASRHKIAIGG 349
+ SH D + +TGST +G+++ A+A+ +L VTLELGGKS DA A+
Sbjct: 233 AISSHMDIDKVAFTGSTGVGRMVMRAAASSNLKKVTLELGGKSPNIVFNDADLDSAAVWT 292
Query: 350 KY 355
Y
Sbjct: 293 NY 294
Score = 44.8 bits (101), Expect = 1e-05
Identities = 32/104 (30%), Positives = 54/104 (51%), Gaps = 1/104 (0%)
Frame = +2
Query: 332 KIAIGGKYDPG-DKFIDLTILTNVDVNDKIMEDEIFGPLLAICVVKDINEALKFINERGK 508
K+ IGGK F++ TIL+NV + + ++EIFGP+LA+ K I EA++ N
Sbjct: 368 KLEIGGKRHGNLGYFVEPTILSNVTEDMAVGKEEIFGPVLAVIKFKTIEEAIRRGNNSTY 427
Query: 509 PLVLYVFSSSEKVHKIFASHTSSGSICFNDTIVFTGVHTLPFGG 640
L V +++ ++ +G++ N + H +PFGG
Sbjct: 428 GLAAGVHTNNITNAIKVSNALEAGTVWVNCYNLLH--HQIPFGG 469
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 60.5 bits (140), Expect = 2e-10
Identities = 45/128 (35%), Positives = 65/128 (50%), Gaps = 7/128 (5%)
Frame = +2
Query: 2 PLALSLLPMAGAIAAGNTVIVKPSEISPASARFIVEYIPKY-LDNDAVIIIEG-GPQETT 175
PL ++ +A A+AAGN +I+K +E +P S + + + V II G G +
Sbjct: 167 PLNMAGWKIAPALAAGNCIIIKSAETTPLSLLYFATLVEEAGFPKGVVNIISGLGTVAGS 226
Query: 176 KLLSHR-FDYIFYTGSTNIGKIIYASAAKHLTPVTLELGGKSRL----QAVLDASRHKIA 340
+ H D I +TGST +G I+ AA +L VTLE GGKS A LD + A
Sbjct: 227 YMAKHPGIDKIAFTGSTKVGVIVQQLAASNLKAVTLECGGKSPFLVFEDADLDQAVKWAA 286
Query: 341 IGGKYDPG 364
+G Y+ G
Sbjct: 287 LGIMYNSG 294
Score = 42.3 bits (95), Expect = 7e-05
Identities = 28/92 (30%), Positives = 45/92 (48%), Gaps = 1/92 (1%)
Frame = +2
Query: 371 FIDLTILTNVDVNDKIMEDEIFGPLLAICVVKDINEALKFINERGKPLVLYVFSSS-EKV 547
FI TI + N I+++EIFGP++AI K +EA++ N L F+ E+
Sbjct: 377 FISPTIFADCSENMTIVKEEIFGPVVAISKFKTEDEAIEKANNTTYGLAAMCFTKDLERA 436
Query: 548 HKIFASHTSSGSICFNDTIVFTGVHTLPFGGV 643
H++ + +G + N T PFGG+
Sbjct: 437 HRV-SDELEAGMVFINST--ENSDIQAPFGGI 465
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 59.7 bits (138), Expect = 4e-10
Identities = 40/104 (38%), Positives = 55/104 (52%), Gaps = 6/104 (5%)
Frame = +2
Query: 2 PLALSLLPMAGAIAAGNTVIVKPSEISPASARFIVEYIPKYLDN-----DAVIIIEGGPQ 166
PL +L P+ A+ AGN ++VK SE++ S E + L + + V I P
Sbjct: 140 PLHNALGPIISALFAGNAIVVKGSELTAWSTHQYCEMVRSLLQSMGHSPELVQCITCLPD 199
Query: 167 ETTKLLSHR-FDYIFYTGSTNIGKIIYASAAKHLTPVTLELGGK 295
L SH +I + GS I K++ ASAAK LTP+ LELGGK
Sbjct: 200 VADHLTSHSGIKHITFIGSQPIAKLVAASAAKQLTPLCLELGGK 243
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 48.0 bits (109), Expect = 1e-06
Identities = 37/120 (30%), Positives = 61/120 (50%), Gaps = 7/120 (5%)
Frame = +2
Query: 2 PLALSLLPMAGAIAAGNTVIVKPSEISPASARFIVEY-----IPKYLDN--DAVIIIEGG 160
P A+ + A+AAG TV+++P+ +P +A + + +P + N A E G
Sbjct: 172 PAAMITRKVGAALAAGCTVVIRPAAETPFTALALAKLAERAGVPAGVLNMVTANSPSEHG 231
Query: 161 PQETTKLLSHRFDYIFYTGSTNIGKIIYASAAKHLTPVTLELGGKSRLQAVLDASRHKIA 340
+ TT L + + +TGSTN+GKI+ ++ L ++LELGG + DA K A
Sbjct: 232 IELTTNPLIRK---VSFTGSTNVGKILAKQSSSTLKKLSLELGGNAPFIVFEDADLEKAA 288
Score = 33.1 bits (72), Expect = 0.042
Identities = 28/106 (26%), Positives = 48/106 (45%), Gaps = 3/106 (2%)
Frame = +2
Query: 335 IAIGGKY--DPGDKFIDLTILTNVDVNDKIMEDEIFGPLLAICVVKDINEALKFINERGK 508
+ GGK + G + + T++ N I E+E FGP+ A+ +E + + N+
Sbjct: 368 VVTGGKVASNLGPMYFEPTVIINAKQGMLISEEETFGPVGALFKFDTEDEVVAWANDSPV 427
Query: 509 PLVLYVFSSS-EKVHKIFASHTSSGSICFNDTIVFTGVHTLPFGGV 643
L Y+FS +V ++ G + N +V + PFGGV
Sbjct: 428 GLAGYLFSKDISRVFRV-GEALQVGMVGCNTGLVSDVLS--PFGGV 470
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 41.9 bits (94), Expect = 9e-05
Identities = 29/106 (27%), Positives = 48/106 (45%), Gaps = 2/106 (1%)
Frame = +2
Query: 332 KIAIGGKYDPGDK--FIDLTILTNVDVNDKIMEDEIFGPLLAICVVKDINEALKFINERG 505
KI +GGK K F + T+L+ V + + +E FGPL ++ D E +++ N+
Sbjct: 362 KITVGGKEISSSKGYFFEPTVLSGVTQDMLVASEETFGPLASVFKFDDTEEVIEWANDSD 421
Query: 506 KPLVLYVFSSSEKVHKIFASHTSSGSICFNDTIVFTGVHTLPFGGV 643
L YVF+++ A G + N I + FGG+
Sbjct: 422 VGLAGYVFTNNLSTMIHVAKELEVGLVGAN--IEMVDEPFISFGGI 465
Score = 38.3 bits (85), Expect = 0.001
Identities = 30/106 (28%), Positives = 50/106 (47%), Gaps = 7/106 (6%)
Frame = +2
Query: 2 PLALSLLPMAGAIAAGNTVIVKPSEISPASARFIVEYIPKYLDNDAVIII-------EGG 160
P A+ A+AAG T I P+ +P +V + D V+ + G
Sbjct: 167 PAAMIARKGGAALAAGCTAIFLPAFRTPYVCLGLVRLAQEAGFPDGVLNVITSSDASAHG 226
Query: 161 PQETTKLLSHRFDYIFYTGSTNIGKIIYASAAKHLTPVTLELGGKS 298
+ TT + + + +TGSTN+GKI+ +A + V++ELGG +
Sbjct: 227 KELTTNPIVRK---VSFTGSTNVGKILMGQSASTIKKVSMELGGNA 269
>SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate
dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 41.1 bits (92), Expect = 2e-04
Identities = 33/130 (25%), Positives = 60/130 (46%), Gaps = 6/130 (4%)
Frame = +2
Query: 293 KSRLQAVLDASRHKIAIGGKYDPGDKF-IDLTILTNVDVNDKIMEDEIFGPLLAICVVKD 469
K L++ S ++ GGK D + F ++ T+L + + I +E+FGP+L++ V +D
Sbjct: 386 KKVLESAASDSEIEVLAGGKADDSEGFFVEPTVLLSKNPKHDIFVNELFGPVLSVYVYED 445
Query: 470 IN-EALKFINERGKP--LVLYVFSSSEKVHKIFAS--HTSSGSICFNDTIVFTGVHTLPF 634
N +A+ + + P L +F+ V + ++G+ ND V PF
Sbjct: 446 DNLDAVCDLIDTTTPYGLTGSIFAQDRVVVRKLTDRLRNAAGNFYINDKCTGAVVGEQPF 505
Query: 635 GGVGSEWYRD 664
GG + D
Sbjct: 506 GGARASGTND 515
Score = 29.5 bits (63), Expect = 0.51
Identities = 20/82 (24%), Positives = 43/82 (52%), Gaps = 3/82 (3%)
Frame = +2
Query: 29 AGAIAAGNTVIVKPSEISPASARFIVEYIPKY-LDNDAVIIIEGGPQETTKL-LSH-RFD 199
A + GN V++KPS+ + S+ + + + L A+ I G E +K+ +H F
Sbjct: 203 AAPLLMGNVVLMKPSDHAVLSSYIVYQIFREAGLPAGALQFIPGDAVEVSKVCFNHPEFA 262
Query: 200 YIFYTGSTNIGKIIYASAAKHL 265
+ +TGST + + ++ + +++
Sbjct: 263 GLHFTGSTAVFRSLWGTIGENV 284
>SPAC22E12.19 ||SPAC2E12.01|histone deacetylase complex subunit
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 661
Score = 31.1 bits (67), Expect = 0.17
Identities = 17/60 (28%), Positives = 30/60 (50%), Gaps = 2/60 (3%)
Frame = +2
Query: 455 CVVKDINEALKFINERGKPLVLYVFSSSEKVHKIFASHTSSGSICFNDTIVF--TGVHTL 628
C+ I++ LK ++E K + S ++ H +SHT+S F ++ F +HTL
Sbjct: 584 CITPSISKILKMVSEDAKSQRIDELSVEDQEHTTHSSHTTSDINAFPNSQSFPRASIHTL 643
>SPAC25H1.03 |mug66|mug66|meiotically upregulated gene
Mug66|Schizosaccharomyces pombe|chr 1|||Manual
Length = 184
Score = 26.2 bits (55), Expect = 4.8
Identities = 10/19 (52%), Positives = 16/19 (84%)
Frame = -2
Query: 390 KIVRSINLSPGSYLPPIAI 334
+IV+++NL SYLPP+A+
Sbjct: 132 QIVQAVNLRSLSYLPPVAM 150
>SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3
subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 821
Score = 25.8 bits (54), Expect = 6.3
Identities = 17/47 (36%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Frame = +3
Query: 561 RHTLAADLFASMIQLCSLEYT-HYRLEELDPSGIGTYHGKMTYDTFT 698
RH L A L S I+ + Y Y L+ LD + HG+ +D FT
Sbjct: 542 RHNLTASL-ESAIRSSNASYEPEYVLKRLDARLLELSHGETGWDVFT 587
>SPAC24H6.13 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 871
Score = 25.4 bits (53), Expect = 8.3
Identities = 20/83 (24%), Positives = 35/83 (42%), Gaps = 2/83 (2%)
Frame = +2
Query: 326 RHKIAIGGKYD--PGDKFIDLTILTNVDVNDKIMEDEIFGPLLAICVVKDINEALKFINE 499
RH + G Y+ P + LT L N +ND+ E+F V+D+ + K + +
Sbjct: 168 RHAMQSSGLYNNLPSSSTMLLTELPNSVLNDEETLHELFPNASEFTCVRDLKKLEKKVKK 227
Query: 500 RGKPLVLYVFSSSEKVHKIFASH 568
R Y + + ++K H
Sbjct: 228 RSDLGNKYESTLNSLINKSVKKH 250
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,226,200
Number of Sequences: 5004
Number of extensions: 69808
Number of successful extensions: 209
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 204
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 341222980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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