Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0299
         (752 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_UPI00015B56E9 Cluster: PREDICTED: similar to conserved ...   182   6e-45
UniRef50_UPI0000D56EA5 Cluster: PREDICTED: similar to CG6210-PB,...   158   1e-37
UniRef50_Q95ST2 Cluster: GH01813p; n=6; Diptera|Rep: GH01813p - ...   138   1e-31
UniRef50_Q5T9L3 Cluster: Integral membrane protein GPR177 precur...   126   6e-28
UniRef50_UPI0000E46F00 Cluster: PREDICTED: similar to LOC362065 ...   122   7e-27
UniRef50_Q4SSK0 Cluster: Chromosome 15 SCAF14367, whole genome s...    71   2e-22
UniRef50_A7S129 Cluster: Predicted protein; n=2; Nematostella ve...    95   2e-18
UniRef50_Q7YWX7 Cluster: Putative uncharacterized protein mig-14...    81   2e-14
UniRef50_Q675R3 Cluster: Putative uncharacterized protein; n=1; ...    47   2e-04

>UniRef50_UPI00015B56E9 Cluster: PREDICTED: similar to conserved
           hypothetical protein; n=1; Nasonia vitripennis|Rep:
           PREDICTED: similar to conserved hypothetical protein -
           Nasonia vitripennis
          Length = 542

 Score =  182 bits (444), Expect = 6e-45
 Identities = 89/140 (63%), Positives = 104/140 (74%)
 Frame = +3

Query: 3   QVFINISHKRQSLPTMCSVRRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQWK 182
           +VFINIS KR  LP+M S RRLHYEG+IYRFKF            +IGF LGQVAEGQWK
Sbjct: 406 KVFINISAKRAVLPSMSSARRLHYEGVIYRFKFLMVATLLCASLTVIGFILGQVAEGQWK 465

Query: 183 WDENIELEYTSAFFTGVYGMWNIYIFALLVLYAPSHKQWPAVEDTSDTQNLSEEIEFTPL 362
           WD++IELE TSAFFTGVYGMWNIY  ALL LYAPSHKQWP VE + +  ++SEEIEF+ L
Sbjct: 466 WDQDIELEMTSAFFTGVYGMWNIYTIALLCLYAPSHKQWP-VEPSEN--SVSEEIEFSRL 522

Query: 363 QERSSEISSLTSFLKKATID 422
               +E+ SLT+F +K  ID
Sbjct: 523 PTEPNEMLSLTAFTRKTAID 542


>UniRef50_UPI0000D56EA5 Cluster: PREDICTED: similar to CG6210-PB,
           isoform B; n=1; Tribolium castaneum|Rep: PREDICTED:
           similar to CG6210-PB, isoform B - Tribolium castaneum
          Length = 541

 Score =  158 bits (384), Expect = 1e-37
 Identities = 81/142 (57%), Positives = 96/142 (67%), Gaps = 2/142 (1%)
 Frame = +3

Query: 3   QVFINISHKRQSLPTMCSVRRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQWK 182
           +VF NIS KR  LP+M   RRLHYEGIIYRF F            +I F L QVAEGQ K
Sbjct: 404 RVFKNISIKRSVLPSMSQARRLHYEGIIYRFNFLMLATVICAAVTVISFILSQVAEGQNK 463

Query: 183 WDENIELEYTSAFFTGVYGMWNIYIFALLVLYAPSHKQWPAVEDTSDTQNLSEEIEFT-- 356
           WDEN+ELE +SA  TGVYGMWN+YI A+L+LYAPSHKQWPA    ++     EE+EF+  
Sbjct: 464 WDENMELELSSALHTGVYGMWNVYICAMLMLYAPSHKQWPAEPICTE----GEEVEFSRL 519

Query: 357 PLQERSSEISSLTSFLKKATID 422
           P     +EISSLTSF  KA+I+
Sbjct: 520 PTDPTPNEISSLTSFASKASIE 541


>UniRef50_Q95ST2 Cluster: GH01813p; n=6; Diptera|Rep: GH01813p -
           Drosophila melanogaster (Fruit fly)
          Length = 594

 Score =  138 bits (334), Expect = 1e-31
 Identities = 68/127 (53%), Positives = 84/127 (66%), Gaps = 11/127 (8%)
 Frame = +3

Query: 75  EGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQWKWDENIELEYTSAFFTGVYGMWNIY 254
           +G+IYRFKF            + GF +GQ+AEGQW W++N+ ++ TSAF TGVYGMWNIY
Sbjct: 468 KGLIYRFKFLMLATLVCAALTVAGFIMGQMAEGQWDWNDNVAIQPTSAFLTGVYGMWNIY 527

Query: 255 IFALLVLYAPSHKQWPAV----EDTSDTQNL-----SEEIEFT--PLQERSSEISSLTSF 401
           IFALL+LYAPSHKQWP +    E T   +N+     SEEIEF+  P     SEISSLTSF
Sbjct: 528 IFALLILYAPSHKQWPTMHHSDETTQSNENIVASAASEEIEFSHLPSDSNPSEISSLTSF 587

Query: 402 LKKATID 422
            +K   D
Sbjct: 588 TRKVAFD 594


>UniRef50_Q5T9L3 Cluster: Integral membrane protein GPR177
           precursor; n=40; Euteleostomi|Rep: Integral membrane
           protein GPR177 precursor - Homo sapiens (Human)
          Length = 541

 Score =  126 bits (304), Expect = 6e-28
 Identities = 59/119 (49%), Positives = 76/119 (63%), Gaps = 1/119 (0%)
 Frame = +3

Query: 3   QVFINISHKRQSLPTMCSVRRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQWK 182
           QVF NIS K+ SLP M  VRRLHYEG+I+RFKF            +I F + QV EG WK
Sbjct: 402 QVFRNISGKQSSLPAMSKVRRLHYEGLIFRFKFLMLITLACAAMTVIFFIVSQVTEGHWK 461

Query: 183 WDENIELEYTSAFFTGVYGMWNIYIFALLVLYAPSHKQWPAVEDTSDT-QNLSEEIEFT 356
           W   + ++  SAFFTG+YGMWN+Y+FAL+ LYAPSHK +   +   D   +  EE++ T
Sbjct: 462 WG-GVTVQVNSAFFTGIYGMWNLYVFALMFLYAPSHKNYGEDQSNGDLGVHSGEELQLT 519


>UniRef50_UPI0000E46F00 Cluster: PREDICTED: similar to LOC362065
           protein, partial; n=3; Strongylocentrotus
           purpuratus|Rep: PREDICTED: similar to LOC362065 protein,
           partial - Strongylocentrotus purpuratus
          Length = 568

 Score =  122 bits (295), Expect = 7e-27
 Identities = 55/104 (52%), Positives = 71/104 (68%), Gaps = 1/104 (0%)
 Frame = +3

Query: 6   VFINISHKRQSLPTMCSVRRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQWKW 185
           VF NIS KR +LP M + RRLHYEG+IYRF+F             I F   Q+ EG WKW
Sbjct: 465 VFKNISMKRAALPHMVAARRLHYEGLIYRFRFFMIFTLICAVMTAIFFIFSQINEGNWKW 524

Query: 186 DEN-IELEYTSAFFTGVYGMWNIYIFALLVLYAPSHKQWPAVED 314
            E+   ++Y+SAFFTGVYGMWN+Y+ A+L+LYAPSHK  P +++
Sbjct: 525 GEDKHNIQYSSAFFTGVYGMWNVYVLAVLMLYAPSHKNKPTLQN 568



 Score = 35.1 bits (77), Expect = 1.9
 Identities = 16/24 (66%), Positives = 18/24 (75%)
 Frame = +3

Query: 6   VFINISHKRQSLPTMCSVRRLHYE 77
           VF NIS KR +LP M + RRLHYE
Sbjct: 371 VFKNISMKRAALPHMVAARRLHYE 394


>UniRef50_Q4SSK0 Cluster: Chromosome 15 SCAF14367, whole genome
           shotgun sequence; n=2; Tetraodon nigroviridis|Rep:
           Chromosome 15 SCAF14367, whole genome shotgun sequence -
           Tetraodon nigroviridis (Green puffer)
          Length = 713

 Score = 71.3 bits (167), Expect(2) = 2e-22
 Identities = 27/47 (57%), Positives = 38/47 (80%)
 Frame = +3

Query: 159 QVAEGQWKWDENIELEYTSAFFTGVYGMWNIYIFALLVLYAPSHKQW 299
           QV EG W W ++  ++  SAFFTG+YGMWN+Y+FA++ LYAPSHK++
Sbjct: 619 QVNEGHWHWGDHT-VQVNSAFFTGIYGMWNLYVFAIMFLYAPSHKRY 664



 Score = 57.2 bits (132), Expect(2) = 2e-22
 Identities = 28/54 (51%), Positives = 32/54 (59%)
 Frame = +3

Query: 3   QVFINISHKRQSLPTMCSVRRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQV 164
           QVF NIS KR SLP M   RRLHYEG+I+RFKF            +I F + QV
Sbjct: 539 QVFRNISGKRMSLPAMSKARRLHYEGLIFRFKFLMLVTLFCAAMTVIFFIISQV 592


>UniRef50_A7S129 Cluster: Predicted protein; n=2; Nematostella
           vectensis|Rep: Predicted protein - Nematostella
           vectensis
          Length = 502

 Score = 95.1 bits (226), Expect = 2e-18
 Identities = 45/96 (46%), Positives = 57/96 (59%), Gaps = 1/96 (1%)
 Frame = +3

Query: 3   QVFINISHKRQSLPTMCSVRRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQWK 182
           +VF NI  KR +   M   RRLHYEG+IYRF+F            +I F +  V E QWK
Sbjct: 393 KVFWNIRGKRATFAKMSRARRLHYEGLIYRFEFLMVITLLCAALTVIFFVISNVNEAQWK 452

Query: 183 W-DENIELEYTSAFFTGVYGMWNIYIFALLVLYAPS 287
           +  E   +E +SA FTG+YGMWN Y+  L+ LYAPS
Sbjct: 453 FGSEESTVEISSALFTGIYGMWNTYVLTLMYLYAPS 488


>UniRef50_Q7YWX7 Cluster: Putative uncharacterized protein mig-14;
           n=2; Caenorhabditis|Rep: Putative uncharacterized
           protein mig-14 - Caenorhabditis elegans
          Length = 549

 Score = 81.4 bits (192), Expect = 2e-14
 Identities = 53/142 (37%), Positives = 73/142 (51%), Gaps = 4/142 (2%)
 Frame = +3

Query: 3   QVFINISHKRQSLPTMCSV-RRLHYEGIIYRFKFXXXXXXXXXXXXIIGFTLGQVAEGQW 179
           +V+  I  KR +     S  RRL  EG+IYRFKF            I  + + Q  E Q 
Sbjct: 409 RVWSTIKSKRSAQIYQTSENRRLKVEGVIYRFKFLMLFTLLCSAFTIAAYFMKQYGEAQL 468

Query: 180 KWDENIE---LEYTSAFFTGVYGMWNIYIFALLVLYAPSHKQWPAVEDTSDTQNLSEEIE 350
             DE  +      TSAFFTG +GM NIY+  LL +YAPSHK +       D +N  +EI 
Sbjct: 469 HGDEARDGFLTGSTSAFFTGAFGMCNIYVLLLLAMYAPSHKHYRGASQLID-ENDDDEIM 527

Query: 351 FTPLQERSSEISSLTSFLKKAT 416
             P   + +E +++T+FLK +T
Sbjct: 528 EDP-SNQHTESNAMTTFLKPST 548


>UniRef50_Q675R3 Cluster: Putative uncharacterized protein; n=1;
           Oikopleura dioica|Rep: Putative uncharacterized protein
           - Oikopleura dioica (Tunicate)
          Length = 513

 Score = 46.8 bits (106), Expect(2) = 2e-04
 Identities = 16/33 (48%), Positives = 26/33 (78%)
 Frame = +3

Query: 195 IELEYTSAFFTGVYGMWNIYIFALLVLYAPSHK 293
           I  +  SA +TG+Y +WN+Y+ ++++LYAPSHK
Sbjct: 429 IRFQVQSAIYTGLYVLWNLYVLSVILLYAPSHK 461



 Score = 21.0 bits (42), Expect(2) = 2e-04
 Identities = 8/14 (57%), Positives = 11/14 (78%)
 Frame = +3

Query: 57  VRRLHYEGIIYRFK 98
           V +LH EG+I RF+
Sbjct: 419 VGKLHAEGLIIRFQ 432


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 647,816,789
Number of Sequences: 1657284
Number of extensions: 12076477
Number of successful extensions: 25763
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 24977
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25750
length of database: 575,637,011
effective HSP length: 99
effective length of database: 411,565,895
effective search space used: 62146450145
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)