BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= an--0288
(541 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49 |... 28 0.77
SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pomb... 27 1.3
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 1.8
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 1.8
SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 27 1.8
SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 27 1.8
SPCC16A11.06c |gpi10||pig-B|Schizosaccharomyces pombe|chr 3|||Ma... 26 3.1
SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 26 4.1
SPBC887.10 |mcs4||two-component response regulator |Schizosaccha... 25 5.4
SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 7.2
SPAC18G6.07c |mra1||ribosome biogenesis protein Mra1|Schizosacch... 25 9.5
SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 25 9.5
>SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 175
Score = 28.3 bits (60), Expect = 0.77
Identities = 13/39 (33%), Positives = 20/39 (51%), Gaps = 7/39 (17%)
Frame = -2
Query: 315 YPQATALAPND-------SDRWTPLIRCRYPNRNDFRAV 220
YP +APN D W PL++ +PN + F++V
Sbjct: 37 YPLQKPVAPNGMRPAKLRKDHWVPLLKVEFPNESMFQSV 75
>SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 287
Score = 27.5 bits (58), Expect = 1.3
Identities = 11/37 (29%), Positives = 20/37 (54%)
Frame = +3
Query: 300 LWPEGKMKDSDNKNRVKECNWSYENEVNVSDEKLREE 410
LWPE + D +N+ +E + E+N +K ++E
Sbjct: 66 LWPETQSSDQENEEEGEELEDAVAKEINSLQKKNKKE 102
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 27.1 bits (57), Expect = 1.8
Identities = 17/46 (36%), Positives = 27/46 (58%), Gaps = 4/46 (8%)
Frame = +3
Query: 291 AQELWPEGKMKD--SDNKNRVKECNWSYENEVN--VSDEKLREENT 416
++++ E MKD ++ R+KE SYENEVN ++ L+E T
Sbjct: 512 SKQVEEESSMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFET 557
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 27.1 bits (57), Expect = 1.8
Identities = 17/57 (29%), Positives = 24/57 (42%), Gaps = 1/57 (1%)
Frame = +3
Query: 315 KMKDSDNKNRVKEC-NWSYENEVNVSDEKLREENTGNGIISRTIRRESETASCEKDN 482
K K +N E N EN++N+ EKL ++N TIR + DN
Sbjct: 737 KSKSLNNSLAALESKNKKLENDLNLLTEKLNKKNADTESFKNTIREAELSKKALNDN 793
>SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 450
Score = 27.1 bits (57), Expect = 1.8
Identities = 23/86 (26%), Positives = 43/86 (50%), Gaps = 2/86 (2%)
Frame = +3
Query: 234 RFDLDNGNESTESIYQNRSAQELWPEGKMKDSDNKNRVKECNWSYENEVNVSDEKLREEN 413
RF+LDN N+S NR +E+ + K K+ D ++E N E + N+ +++ +
Sbjct: 46 RFELDNTNKSI-----NRVQKEIGLKMKAKE-DASELLEEKNSLTERKKNLIEQETAKNK 99
Query: 414 TGNGIISR--TIRRESETASCEKDND 485
++S I +S S ++DN+
Sbjct: 100 EMLNVVSSIGNIVHDSVPVSMDEDNN 125
>SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase
Snf21|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1199
Score = 27.1 bits (57), Expect = 1.8
Identities = 12/19 (63%), Positives = 15/19 (78%)
Frame = -3
Query: 344 AILVVRILHLTLRPQLLRR 288
++LV+R LH LRP LLRR
Sbjct: 626 SLLVIRRLHKVLRPFLLRR 644
>SPCC16A11.06c |gpi10||pig-B|Schizosaccharomyces pombe|chr
3|||Manual
Length = 506
Score = 26.2 bits (55), Expect = 3.1
Identities = 13/27 (48%), Positives = 15/27 (55%)
Frame = -3
Query: 434 TYDSIACVFLS*FLIGHVNFVFVAPIA 354
T +I CVF LIGH FV PI+
Sbjct: 309 TAGTILCVFFPYSLIGHKELRFVYPIS 335
>SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 584
Score = 25.8 bits (54), Expect = 4.1
Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 3/59 (5%)
Frame = +3
Query: 237 FDLDNGNESTESIYQNRSAQELWPEGKMKDSD---NKNRVKECNWSYENEVNVSDEKLR 404
FD ++ + + I Q +A E P KM S+ ++NR ++ W +NE D LR
Sbjct: 131 FDPNDPSAAAFYIAQQVAAIEHPPPRKMTSSEKTRSENRERKKRWREQNEERNKDNDLR 189
>SPBC887.10 |mcs4||two-component response regulator
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 522
Score = 25.4 bits (53), Expect = 5.4
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%)
Frame = -2
Query: 330 PNPSSYPQATALAPNDSDRWTPLIRCRYPNRNDFR---AVGGPDNEL*FR 190
PN SS Q+ L+PN+S + ++ YP+ DF V PD + R
Sbjct: 56 PNASSELQSRELSPNESILF--VMETYYPHGQDFNDALLVASPDTSVALR 103
>SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr
1|||Manual
Length = 566
Score = 25.0 bits (52), Expect = 7.2
Identities = 15/54 (27%), Positives = 24/54 (44%)
Frame = +3
Query: 330 DNKNRVKECNWSYENEVNVSDEKLREENTGNGIISRTIRRESETASCEKDNDIK 491
DN ++E S+ENE SD+ + E G I E + +D+ I+
Sbjct: 294 DNNIVLREVKVSFENEKIESDDLDKNEEISEGEEYTPIEESKEPITVRRDSVIQ 347
>SPAC18G6.07c |mra1||ribosome biogenesis protein
Mra1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 359
Score = 24.6 bits (51), Expect = 9.5
Identities = 23/92 (25%), Positives = 40/92 (43%)
Frame = +3
Query: 255 NESTESIYQNRSAQELWPEGKMKDSDNKNRVKECNWSYENEVNVSDEKLREENTGNGIIS 434
++S+E+I +A EL + K + + +++ E S E L ++
Sbjct: 28 SQSSETITSGETASELMQDEKEQSNGAVGSIEDEELQRLRENQASVEALSKK-------P 80
Query: 435 RTIRRESETASCEKDNDIKPALLNIDPDSASS 530
+I RE + E DN +K DP+ ASS
Sbjct: 81 ESIDRELGVEALEIDNVVKSDEEKEDPNGASS 112
>SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 595
Score = 24.6 bits (51), Expect = 9.5
Identities = 11/30 (36%), Positives = 16/30 (53%)
Frame = -3
Query: 497 GRLNIVVLFTRSRLALASYCATYDSIACVF 408
G L V + R+ ++S C Y S+AC F
Sbjct: 314 GDLAPSVAISLQRIKVSSLCILYPSLACCF 343
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,170,645
Number of Sequences: 5004
Number of extensions: 44222
Number of successful extensions: 134
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 134
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 221892220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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