BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= an--0288 (541 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49 |... 28 0.77 SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pomb... 27 1.3 SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 1.8 SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 27 1.8 SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr ... 27 1.8 SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|... 27 1.8 SPCC16A11.06c |gpi10||pig-B|Schizosaccharomyces pombe|chr 3|||Ma... 26 3.1 SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces p... 26 4.1 SPBC887.10 |mcs4||two-component response regulator |Schizosaccha... 25 5.4 SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|c... 25 7.2 SPAC18G6.07c |mra1||ribosome biogenesis protein Mra1|Schizosacch... 25 9.5 SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces p... 25 9.5 >SPBC18H10.17c |||mitochondrial ribosomal protein subunit Mrp49 |Schizosaccharomyces pombe|chr 2|||Manual Length = 175 Score = 28.3 bits (60), Expect = 0.77 Identities = 13/39 (33%), Positives = 20/39 (51%), Gaps = 7/39 (17%) Frame = -2 Query: 315 YPQATALAPND-------SDRWTPLIRCRYPNRNDFRAV 220 YP +APN D W PL++ +PN + F++V Sbjct: 37 YPLQKPVAPNGMRPAKLRKDHWVPLLKVEFPNESMFQSV 75 >SPBC25H2.10c |||tRNA acetyltransferase |Schizosaccharomyces pombe|chr 2|||Manual Length = 287 Score = 27.5 bits (58), Expect = 1.3 Identities = 11/37 (29%), Positives = 20/37 (54%) Frame = +3 Query: 300 LWPEGKMKDSDNKNRVKECNWSYENEVNVSDEKLREE 410 LWPE + D +N+ +E + E+N +K ++E Sbjct: 66 LWPETQSSDQENEEEGEELEDAVAKEINSLQKKNKKE 102 >SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces pombe|chr 1|||Manual Length = 670 Score = 27.1 bits (57), Expect = 1.8 Identities = 17/46 (36%), Positives = 27/46 (58%), Gaps = 4/46 (8%) Frame = +3 Query: 291 AQELWPEGKMKD--SDNKNRVKECNWSYENEVN--VSDEKLREENT 416 ++++ E MKD ++ R+KE SYENEVN ++ L+E T Sbjct: 512 SKQVEEESSMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFET 557 >SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1044 Score = 27.1 bits (57), Expect = 1.8 Identities = 17/57 (29%), Positives = 24/57 (42%), Gaps = 1/57 (1%) Frame = +3 Query: 315 KMKDSDNKNRVKEC-NWSYENEVNVSDEKLREENTGNGIISRTIRRESETASCEKDN 482 K K +N E N EN++N+ EKL ++N TIR + DN Sbjct: 737 KSKSLNNSLAALESKNKKLENDLNLLTEKLNKKNADTESFKNTIREAELSKKALNDN 793 >SPAC29A4.15 |||serine-tRNA ligase|Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 27.1 bits (57), Expect = 1.8 Identities = 23/86 (26%), Positives = 43/86 (50%), Gaps = 2/86 (2%) Frame = +3 Query: 234 RFDLDNGNESTESIYQNRSAQELWPEGKMKDSDNKNRVKECNWSYENEVNVSDEKLREEN 413 RF+LDN N+S NR +E+ + K K+ D ++E N E + N+ +++ + Sbjct: 46 RFELDNTNKSI-----NRVQKEIGLKMKAKE-DASELLEEKNSLTERKKNLIEQETAKNK 99 Query: 414 TGNGIISR--TIRRESETASCEKDND 485 ++S I +S S ++DN+ Sbjct: 100 EMLNVVSSIGNIVHDSVPVSMDEDNN 125 >SPAC1250.01 |snf21|SPAC29A4.21|ATP-dependent DNA helicase Snf21|Schizosaccharomyces pombe|chr 1|||Manual Length = 1199 Score = 27.1 bits (57), Expect = 1.8 Identities = 12/19 (63%), Positives = 15/19 (78%) Frame = -3 Query: 344 AILVVRILHLTLRPQLLRR 288 ++LV+R LH LRP LLRR Sbjct: 626 SLLVIRRLHKVLRPFLLRR 644 >SPCC16A11.06c |gpi10||pig-B|Schizosaccharomyces pombe|chr 3|||Manual Length = 506 Score = 26.2 bits (55), Expect = 3.1 Identities = 13/27 (48%), Positives = 15/27 (55%) Frame = -3 Query: 434 TYDSIACVFLS*FLIGHVNFVFVAPIA 354 T +I CVF LIGH FV PI+ Sbjct: 309 TAGTILCVFFPYSLIGHKELRFVYPIS 335 >SPBC17D1.01 ||SPBC17D11.09|sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 584 Score = 25.8 bits (54), Expect = 4.1 Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 3/59 (5%) Frame = +3 Query: 237 FDLDNGNESTESIYQNRSAQELWPEGKMKDSD---NKNRVKECNWSYENEVNVSDEKLR 404 FD ++ + + I Q +A E P KM S+ ++NR ++ W +NE D LR Sbjct: 131 FDPNDPSAAAFYIAQQVAAIEHPPPRKMTSSEKTRSENRERKKRWREQNEERNKDNDLR 189 >SPBC887.10 |mcs4||two-component response regulator |Schizosaccharomyces pombe|chr 2|||Manual Length = 522 Score = 25.4 bits (53), Expect = 5.4 Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 3/50 (6%) Frame = -2 Query: 330 PNPSSYPQATALAPNDSDRWTPLIRCRYPNRNDFR---AVGGPDNEL*FR 190 PN SS Q+ L+PN+S + ++ YP+ DF V PD + R Sbjct: 56 PNASSELQSRELSPNESILF--VMETYYPHGQDFNDALLVASPDTSVALR 103 >SPAP14E8.02 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 25.0 bits (52), Expect = 7.2 Identities = 15/54 (27%), Positives = 24/54 (44%) Frame = +3 Query: 330 DNKNRVKECNWSYENEVNVSDEKLREENTGNGIISRTIRRESETASCEKDNDIK 491 DN ++E S+ENE SD+ + E G I E + +D+ I+ Sbjct: 294 DNNIVLREVKVSFENEKIESDDLDKNEEISEGEEYTPIEESKEPITVRRDSVIQ 347 >SPAC18G6.07c |mra1||ribosome biogenesis protein Mra1|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 24.6 bits (51), Expect = 9.5 Identities = 23/92 (25%), Positives = 40/92 (43%) Frame = +3 Query: 255 NESTESIYQNRSAQELWPEGKMKDSDNKNRVKECNWSYENEVNVSDEKLREENTGNGIIS 434 ++S+E+I +A EL + K + + +++ E S E L ++ Sbjct: 28 SQSSETITSGETASELMQDEKEQSNGAVGSIEDEELQRLRENQASVEALSKK-------P 80 Query: 435 RTIRRESETASCEKDNDIKPALLNIDPDSASS 530 +I RE + E DN +K DP+ ASS Sbjct: 81 ESIDRELGVEALEIDNVVKSDEEKEDPNGASS 112 >SPCC1620.12c |||GTPase activating protein |Schizosaccharomyces pombe|chr 3|||Manual Length = 595 Score = 24.6 bits (51), Expect = 9.5 Identities = 11/30 (36%), Positives = 16/30 (53%) Frame = -3 Query: 497 GRLNIVVLFTRSRLALASYCATYDSIACVF 408 G L V + R+ ++S C Y S+AC F Sbjct: 314 GDLAPSVAISLQRIKVSSLCILYPSLACCF 343 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,170,645 Number of Sequences: 5004 Number of extensions: 44222 Number of successful extensions: 134 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 133 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 134 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 221892220 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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