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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= an--0285
         (792 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz...    66   7e-12
SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz...    44   2e-05
SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac...    37   0.004
SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces p...    31   0.14 
SPCC1322.16 |phb2||prohibitin Phb2|Schizosaccharomyces pombe|chr...    27   4.1  
SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr...    27   4.1  
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p...    26   7.1  
SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10 |Schizos...    25   9.4  

>SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 216

 Score = 65.7 bits (153), Expect = 7e-12
 Identities = 45/152 (29%), Positives = 72/152 (47%), Gaps = 6/152 (3%)
 Frame = +1

Query: 223 CLKEELHANVLVAGEYDVTEIQG--QRVDYIIKDSKGHILSQKDTVTKGK-FSFVTENYD 393
           CL+E +    LV      T   G  Q +  +I DS G+  S    V   K  +F  +   
Sbjct: 36  CLREYVGEKNLVIVNLKTTGNMGDGQTLSMMITDSSGNTHSSIQNVLGEKSVAFDVDASA 95

Query: 394 MFEVCFISKVPSERRGIPHQ---VSLDIKIGIEAKTYEGIGEAAKLKPMEVELKRLEDLS 564
           M ++CF++ +        H+   V L+  +G +A  Y  + +A  L+P+E +++R  D  
Sbjct: 96  MLDICFLNTLTPGAIESEHKKRSVKLEFTVGADADDYSSLQKANNLEPVEADIRRARDFI 155

Query: 565 EAIVQDFTLMRKREEEMRDTNESTNNRVLFFS 660
           E I      ++ RE   R+TNESTN RV  F+
Sbjct: 156 EEIKGKIYYLQAREARFRNTNESTNERVKNFA 187


>SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 199

 Score = 44.4 bits (100), Expect = 2e-05
 Identities = 36/164 (21%), Positives = 67/164 (40%)
 Frame = +1

Query: 199 SLAPNTHKCLKEELHANVLVAGEYDVTEIQGQRVDYIIKDSKGHILSQKDTVTKGKFSFV 378
           +L P+  +C  E L  N  ++  Y       Q V   I +  G I+ Q+   +  ++SF 
Sbjct: 25  TLKPHQRECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVPNSMAQYSFT 84

Query: 379 TENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYEGIGEAAKLKPMEVELKRLED 558
            +N   +  CF +             S ++   +    Y    +     P+  ++++L D
Sbjct: 85  VKNPGKYMYCFYNDALDGE-------SKEVLFNVHGVIYISDEDLDANNPLLGKVRQLHD 137

Query: 559 LSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSIFSDGVPLGV 690
               +  +      RE   R+T ESTN+RV ++SI    + + V
Sbjct: 138 TISKVKHEQEYFVARERIHRNTAESTNDRVKWWSILQTVILVSV 181


>SPBC16E9.09c |||COPII vesicle coat component
           Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 215

 Score = 36.7 bits (81), Expect = 0.004
 Identities = 26/115 (22%), Positives = 49/115 (42%)
 Frame = +1

Query: 319 SKGHILSQKDTVTKGKFSFVTENYDMFEVCFISKVPSERRGIPHQVSLDIKIGIEAKTYE 498
           +  H++ + D  +     F T N     +C++S           +  L + +  + K   
Sbjct: 75  ANNHLIYKNDLRSNEAVRFTTTNEGTHRICYLSTSDGAWFSST-KTRLHVDLASDDKYNL 133

Query: 499 GIGEAAKLKPMEVELKRLEDLSEAIVQDFTLMRKREEEMRDTNESTNNRVLFFSI 663
                 + K +   +  L    E+I+ +  L R RE   RDT+ES N+RV+ ++I
Sbjct: 134 IASSEDEAKDLTDRVAALNTRLESILGEQMLQRSREMTFRDTSESANSRVVRWTI 188


>SPCC11E10.08 |rik1||silencing protein Rik1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1040

 Score = 31.5 bits (68), Expect = 0.14
 Identities = 16/57 (28%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
 Frame = +1

Query: 277 TEIQGQRVDYIIKDSKGHILSQKDTVT-KGKFSFVTENYDMFEVCFISKVPSERRGI 444
           T ++GQ++ YI+ D+ G++     ++  K K+S V E   +    FI+ + S   G+
Sbjct: 259 TPLKGQKMHYIVCDTNGYLFGVYSSILGKNKWSLVMERLPIPPFDFITSLNSIHEGL 315


>SPCC1322.16 |phb2||prohibitin Phb2|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 279

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 13/29 (44%), Positives = 18/29 (62%)
 Frame = +1

Query: 481 EAKTYEGIGEAAKLKPMEVELKRLEDLSE 567
           E +  + IGEA K KP  +EL++LE   E
Sbjct: 226 EGRAAQLIGEAIKNKPGFIELRKLETARE 254


>SPBC2D10.14c |myo51||myosin type V|Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1471

 Score = 26.6 bits (56), Expect = 4.1
 Identities = 18/73 (24%), Positives = 32/73 (43%), Gaps = 4/73 (5%)
 Frame = -2

Query: 446  GIPLLSDGTLDIKQTSNIS*FSVTNENFPFVTVSFCDKMCPFESLII*STLCPCISVT-- 273
            GI  ++   L + Q+  +      + N   V +S C+ + PFE + + +   PC      
Sbjct: 1344 GISYVNSSLLPLMQSPLVFCLRKNDANDLDVILSVCNLLSPFEVVCLLNRYQPCAGENPL 1403

Query: 272  --SYSPATNTLAC 240
              S+S A   L+C
Sbjct: 1404 PKSFSKAVEALSC 1416


>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 425

 Score = 25.8 bits (54), Expect = 7.1
 Identities = 8/20 (40%), Positives = 12/20 (60%)
 Frame = +1

Query: 211 NTHKCLKEELHANVLVAGEY 270
           NTH+C++     N  + GEY
Sbjct: 258 NTHRCIERSTDCNCPICGEY 277


>SPBC23E6.04c |utp10||U3 snoRNP-associated protein Utp10
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1649

 Score = 25.4 bits (53), Expect = 9.4
 Identities = 13/28 (46%), Positives = 17/28 (60%)
 Frame = +1

Query: 529 MEVELKRLEDLSEAIVQDFTLMRKREEE 612
           M++ L RLE LSE   + F  + KRE E
Sbjct: 654 MQILLPRLEQLSEFNSEKFKTVSKREVE 681


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,190,645
Number of Sequences: 5004
Number of extensions: 66581
Number of successful extensions: 165
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 385381248
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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